Conserved Protein Domain Family

cd08059: MPN_prok_mb 
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Mpr1p, Pad1p N-terminal (MPN) domains with catalytic isopeptidase activity (metal-binding); prokaryotic
This family contains bacterial and archaeal MPN (also known as Mov34, PAD-1, JAMM, JAB, MPN+)-like domains. These catalytically active domains contain the signature JAB1/MPN/Mov34 metalloenzyme (JAMM) motif, EXnHS/THX7SXXD, which is involved in zinc ion coordination and provides the active site for isopeptidase activity for the release of ubiquitin from ubiquitinated proteins (thus having deubiquitinating (DUB) activity) that are tagged for degradation. The JAMM proteins likely hydrolyze ubiquitin conjugates in a manner similar to thermolysin, in which the zinc-polarized aqua ligand serves as the nucleophile, compared with the classical DUBs that do so with a cysteine residue in the active site.
PSSM-Id: 163690
View PSSM: cd08059
Aligned: 5 rows
Threshold Bit Score: 123.441
Threshold Setting Gi: 158430296
Created: 4-Nov-2009
Updated: 2-Oct-2020
Aligned Rows:
MPN+ (JAMM)Zinc-binding
Conserved site includes 5 residues -Click on image for an interactive view with Cn3D
Feature 1:MPN+ (JAMM) motif
  • Structure:1R5X: Archaeoglobus fulgidus JAMM-like domain
    View structure with Cn3D
  • Comment:Based on literature and mutation studies

Sequence Alignment
Format: Row Display: Color Bits: Type Selection:
Feature 1                     #                                                            # #
1R5X_A     18 GLLKTILEAAksahpdEFIALLSGskd-----vmdELIFlpfvsgsvsavi-----------hldmlpigmkvFGTVHSH 81  Archaeoglobus fulg...
NP_287597  49 QTESAILVHArrcapaESCGFVIGtpegeryqpcvNISAepeayfriape------------dwlqaemqgeiVALVHSH 116 Escherichia coli O...
1OI0_A      9 GLLKTILEAAksahpdEFIALLSGskd-----vmdELIFlpfvsgsvsavi-----------hldmlpigmkvFGTVHSH 72  Archaeoglobus fulg...
2KCQ_A      6 DILDQIRVHGadaypeEGCGFLLGtvtddgdnrvaALHRatnrrseqrtrryeltaddyraadaaaqeqgldvVGVYHSH 85  Salinibacter ruber...
2QLC_A     10 DVFEYMKGRIpde-tkEHLFVLFLstkn----qilRHETitigtltaslihpr-------eifkaairesahsIILVHNH 77  Chlorobium tepidum...
Feature 1            #  #                                     
1R5X_A     82 PspscrPSEEDLSLFTrf----------gkYHIIVCypyd-ensWKCY 118 Archaeoglobus fulgidus DSM 4304
NP_287597 117 PgglpwLSEADRRLQIks----------alPWWLVCrge--ihkFRCV 152 Escherichia coli O157:H7 EDL933
1OI0_A     73 PspscrPSEEDLSLFTrf----------gkYHIIVCypyd-ensWKCY 109 Archaeoglobus fulgidus
2KCQ_A     86 PdhparPSATDLEEATfp----------gfTYVIVSvrdgapeaLTAW 123 Salinibacter ruber DSM 13855
2QLC_A     78 PsgdvqPSNADKQVTSilkkagdllqiellDHVIVGnn-----dWFSF 120 Chlorobium tepidum TLS

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