2ZNR,1OI0,2QLC,2ZNV,3JCK,4WSN,5LD9,6GVW


Conserved Protein Domain Family
MPN_mb

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cd08058: MPN_mb (This model is not part of the current CDD release)
MPN domain: metal-binding family
This family contains MPN (also known as Mov34, PAD-1, JAMM, JAB, MPN+) domains found in proteins with a variety of functions, including AMSH (associated molecule with the Src homology 3 domain (SH3) of STAM), 2A-DUB (histone H2A deubiquitinase), BRCC36 (BRCA1/BRCA2-containing complex subunit 36), as well as Rpn11 (regulatory particle number 11) and CSN5 (COP9 signalosome complex subunit 5). These domains contain the signature JAB1/MPN/Mov34 metalloenzyme (JAMM) motif, EXnHS/THX7SXXD, which is involved in zinc ion coordination and provides the active site for isopeptidase activity. Rpn11 is responsible for substrate deubiquitination during proteasomal degradation. It is essential for maintaining a correct cell cycle and normal mitochondrial morphology and physiology. CSN5 is critical for nuclear export and the degradation of several tumor suppressor proteins, including p53, p27, and Smad4. Over-expression of CSN5 has been implicated in cancer initiation and progression. AMSH specifically cleaves Lys 63 and not Lys48-linked polyubiquitin (poly-Ub) chains, thus facilitating the recycling and subsequent trafficking of receptors to the cell surface. It is involved in the degradation of EGF receptor (EGFR) and possibly other ubiquitinated endocytosed proteins. BRCC36 is part of the BRCA1/BRCA2/BARD1-containing nuclear complex that displays an E3 ubiquitin ligase activity; it is targeted to DNA damage foci after irradiation. 2A-DUB is specific for monoubiquitinated H2A (uH2A), regulating transcription by coordinating histone acetylation and deubiquitination, and destabilizing the association of linker histone H1 with nucleosomes. It is a positive regulator of androgen receptor (AR) transactivation activity on a reporter gene and serves as a marker in prostate tumors.
Statistics
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PSSM-Id: 501528
Aligned: 10 rows
Threshold Bit Score: 96.7412
Created: 30-Nov-2009
Updated: 30-Oct-2024
Structure
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Program:
Drawing:
Aligned Rows:
 
Zn binding siteMPN+ (JAMM)
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1: Zn binding site [ion binding site], 3 residue positions
Conserved feature residue pattern:H H DClick to see conserved feature residue pattern help
Evidence:
  • Structure:2ZNR: Human DUB domain of AMSH-like protein binds Zn ion

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                     
2ZNR_A     16 DLCHKFLQLAEsntvrgiETCGILCGKlthn-------------------------eftITHVIVPKqsagpdycdmenv 70  human
1OI0_A      9 GLLKTILEAAKsah--pdEFIALLSGSkd-----------------------------vMDELIFLPfvsgsvsavi--- 54  Archaeoglobus fulg...
2QLC_A     10 DVFEYMKGRIPde---tkEHLFVLFLStk----------------------------nqILRHETITigtltaslihpr- 57  Chlorobium tepidum...
2ZNV_A     16 DLCHKFLQLAEsntvrgiATCGILCGKlthn-------------------------eftITHVIVPKqsagpdycdmenv 70  human
3JCK_G     32 IALLKMLKHGRagv--pmEVMGLMLGEfvdd------------------------ytvnVVDVFAMPqsgtgvsveavdd 85  Saccharomyces cere...
4WSN_E     51 LALLKMVMHARsgg--nlEVMGLMLGKvdge-------------------------tmiIMDSFALPvegtetrvnaqaa 103 human
5LD9_A     18 QHYLRAILKVVsss--svEVCGFLFGKen-----------------------------rVLKVRFIRnrlnspvefemdp 66  Pyrococcus furiosu...
6GVW_B     21 DAFLVCLNHALste--keEVMGLCIGElnddirsdskftytgtemrtvqekmdtirivhIHSVIILRrsdkrkdrveisp 98  house mouse
NP_287597  49 QTESAILVHARrca--paESCGFVIGTpege------------------------ryqpCVNISAEPeayfriaped--- 99  Escherichia coli O...
Q5VVJ2    582 EALLIMDLHAHvs---maEVIGLLGGRyse--------------------------vdkVVEVCAAEpcnslstglqcem 632 human
Feature 1                                # #          #                                       
2ZNR_A     71 ---------eelfnvqdqhdllTLGWIHTHPtqTAFLSSVDLHTHCSYqlml-----peaIAIVCSpk---------hkD 127 human
1OI0_A     55 ------------hldmlpigmkVFGTVHSHPspSCRPSEEDLSLFTRFg----------kYHIIVCypy-------denS 105 Archaeoglobus fulg...
2QLC_A     58 ----------eifkaairesahSIILVHNHPsgDVQPSNADKQVTSILkkagdllqiellDHVIVGn-----------nD 116 Chlorobium tepidum...
2ZNV_A     71 ---------eelfnvqdqhdllTLGWIHTHPtqTAFLSSVDLHTHCSYqlml-----peaIAIVCSpk---------hkD 127 human
3JCK_G     86 vfq----akmmdmlkqtgrdqmVVGWYHSHPgfGCWLSSVDVNTQKSFeqln-----sraVAVVVDpiqsv----kgkvV 152 Saccharomyces cere...
4WSN_E    104 ayey--maayienakqvgrlenAIGWYHSHPgyGCWLSGIDVSTQMLNqqfq-----epfVAVVIDptrti---sagkvN 173 human
5LD9_A     67 ee------mlkaleeaeqenleVVGIFHSHIacPPIPSGKDLEGMKRWp----------vIWLIVNek----------gE 120 Pyrococcus furiosu...
6GVW_B     99 eqlsaasteaerlaeltgrpmrVVGWYHSHPhiTVWPSHVDVRTQAMYqmmd-----qgfVGLIFScfiedkntktgrvL 173 house mouse
NP_287597 100 -------------wlqaemqgeIVALVHSHPggLPWLSEADRRLQIKSa----------lPWWLVCrge--------ihK 148 Escherichia coli O...
Q5VVJ2    633 dpv----sqtqasetlavrgfsVIGWYHSHPafDPNPSLRDIDTQAKYqsyfsr-ggakfIGMIVSpyn-------rnnP 700 human
Feature 1         
2ZNR_A    128 TGIF 131 human
1OI0_A    106 WKCY 109 Archaeoglobus fulgidus
2QLC_A    117 WFSF 120 Chlorobium tepidum TLS
2ZNV_A    128 TGIF 131 human
3JCK_G    153 IDAF 156 Saccharomyces cerevisiae S288C
4WSN_E    174 LGAF 177 human
5LD9_A    121 YKAW 124 Pyrococcus furiosus DSM 3638
6GVW_B    174 YTCF 177 house mouse
NP_287597 149 FRCV 152 Escherichia coli O157:H7 EDL933
Q5VVJ2    701 LPYS 704 human

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