2P87,2P8R,2OG4


Conserved Protein Domain Family
MPN_PRP8

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cd08056: MPN_PRP8 
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Mpr1p, Pad1p N-terminal (MPN) domains without isopeptidase activity found in splicing factor Prp8
Members of this family are found in pre-mRNA-processing factor 8 (Prp8) which is a critical splicing factor, interacting with several other spliceosomal proteins, snRNAs, and the pre-mRNA, thus organizing and stabilizing the spliceosome catalytic core. Prp8 is one of the largest and most highly conserved of nuclear proteins, occupying a central position in the catalytic core of the spliceosome. Its C-terminal domain exhibits a JAB1/MPN-like core similar to deubiquitinating enzymes, but does not show catalytic isopeptidase activity, possibly because the putative isopeptidase center is covered by insertions and terminal appendices that are grafted onto this core, thus impairing the metal binding site. It is proposed that this domain is a protein interaction domain instead of a Zn(2+)-dependent metalloenzyme as proposed for some MPN proteins. The DEAD-box protein Brr2 and the GTPase Snu114 bind to the Prp8 C-terminus, a region where mutations in human Prp8 (hPrp8) cause a severe form of the genetic disorder retinitis pigmentosa, RP13, which leads to progressive photoreceptor degeneration in the retina and eventual blindness. At the N-terminus of Prp8, there are several domains, including a highly variable nuclear localization signal (NLS) motif rich in prolines, a conserved RNA recognition motif (RRM), and U5 and U6 snRNA binding sites.
Statistics
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PSSM-Id: 163687
View PSSM: cd08056
Aligned: 45 rows
Threshold Bit Score: 279.512
Threshold Setting Gi: 67475030
Created: 21-Jul-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
2P87_A          4 FASRTEWRVRAISSTNLHLRt------qHIYV-------NSDDVKDTGY----TYILPKNILKKFITISDLRTQIAGFMY 66   nematode
NP_983156    2137 FSSKNEWRKRAVASSLLYLRl------kNIYV-------SSEDFIEEKS----IIVLPRNLLKKFVGIADVKIQIAGFLY 2199 Ashbya gossyp...
XP_451233    2146 FSSKNAWRSNAISNSLLHLKl------kNIYV-------TAEDFVEDKD----VLVFPRNLLKKFVTIADVDVQIAGYMY 2208 Kluyveromyces...
XP_954981    2529 VTTQSPHEQQVFASKTDWRNrcl--asgTLHL-------RAKHIYVVPVeseqVIVLPNNLIKKLISIADLRTQVGAYLY 2599 Theileria ann...
XP_447350    2147 FNSKSEWKKSALANSLLHIRl------kNIYV-------NAGEFIEEKP----VYIFPKNILEKLVQISDVKLQVAAYIY 2209 Candida glabr...
XP_001610701 2074 VTTQTPHEQKVFASKTDWRNrclaagllHRRLe-----gLSVESVDSDD----TLVIPLNLIRKLVMVSDLRTTVAVYLY 2144 Babesia bovis...
CAA73186     2129 STSASNWRARSLANATVFGEg------tTVEI-------DHSGVTGSSD----QLIFPQELLKILFPCFDVQAQFCAYLF 2191 Trypanosoma b...
XP_001712807 2001 FKSKKNWKSRLQKKINTFAIv------rNLFIn------KNEKFNANSR----IFLISKHLVLKFASISDTCSETFGYIY 2064 Bigelowiella ...
XP_653245    1998 VTTSTPYEQKVFAATQNWRQr-------AIEAsklldkmNNLFVENEGEg--nPIVIPKNIIKGIIQIADTKIEIGGILF 2068 Entamoeba his...
XP_001469202 2145 TALNNNWRPRSLADAAFLDE--------NTPI-------TFDAAGATGAs--dQLIFSEDAIQKLLACCDVKVQCCAYMF 2207 Leishmania in...
2P87_A         67 GVSPpd--------npQVKEIRCIVLVPQTGSHQQVNLPtql--pdHELLRDFE--PLGWMHTQPNElpqLSPQDVTTHA 134  nematode
NP_983156    2200 GQSPpe--------hpNIKEIRKIVLCPQLGSNQTVQLSnlp--gqSEYLEDLE--LLGWINTFAQDkeyMLPADITTHV 2267 Ashbya gossyp...
XP_451233    2209 GSPVkd--------hpNVKEIKTIALVPQLGSSATVRISppp--ssSKYLNDME--LLGWIHTQKVDskfMSPSEVITHS 2276 Kluyveromyces...
XP_954981    2600 SKVEkn--------ehTVYSIVCMVLVPQVGTHKTIVLPklk--peHEALSELV--PIGWIFTRPNEg-eIEQQTLEMHQ 2666 Theileria ann...
XP_447350    2210 GSSPkd--------hpNVNEIQTVVLVPQISTDRTVKFIghpnhddYEELEGLK--LLGWVNIQHEDfkfMGPSALMNHA 2279 Candida glabr...
XP_001610701 2145 GKISdvesgdprgassNVREVACMVFVPQVGNAFTADLPkqa--pqHESLDGLE--PLGWLITRPTEg-pVPKEAVESHK 2219 Babesia bovis...
CAA73186     2192 GQTLpd--------spNVKEVLCIMVPPQKSSAVEYTTPsci-phdHPILTENHlsLLGVLRCSGGEp-sIHSRDVAIHG 2261 Trypanosoma b...
XP_001712807 2065 GKDSie--------fnNISEIICISIPPQLVSGLSLAMAikh--ipHKSTDDFE--ILGWIHLTAYTenqILSRNLRYHA 2132 Bigelowiella ...
XP_653245    2069 GQEStk--------nkGIMEVQCCVLVPQYATNKYIVFAqhc--pqHEELHNMK--AIGWIHTCSGEp-kLTVYDAVMNS 2135 Entamoeba his...
XP_001469202 2208 GHALpd--------spNIKEVLCVMIPPQFGTAVEARTPpri-pfdAAALQEANlsFLGLMRIGESEa-qLTSHDLALQA 2277 Leishmania in...
2P87_A        135 KLltdnis--wdgektVMITCSFTpgSVSLTAYKLTpSGYEWGKantdkgnn--pkgymptHYEKVQMLLSDRFLGYFMV 210  nematode
NP_983156    2268 RTfgra------ntevINLTVGLIpgAISLSAFTVTkEGLDWGMenkdifdp-spegfdpsFSEHAQLLLSERIMGNFLV 2340 Ashbya gossyp...
XP_451233    2277 KMfsdh------nesaVDLCINLIpgAVSITGYTLStTGFNWGLqskditdp-spegfeasFSETAQILLSERIMGNFLV 2349 Kluyveromyces...
XP_954981    2667 KMindfg----wdptaVMTTCTFTpgSCAISARRLV-NALEKPQn---------------lQLEKVQVLVSDTFKGFFLV 2726 Theileria ann...
XP_447350    2280 SHfsnsk-----hdpiMISICSVPg-SISISGFTASiKGLAWAMqnkdtvse-ipegfepnYSENAQLMLSDRISGNFFV 2352 Candida glabr...
XP_001610701 2220 RLqsdms----wdvvaVLCTCTFVrgSCSFLAYSLTpDALVSKDisn-----------vssLLTPVQVLVSDKYKGFFLV 2284 Babesia bovis...
CAA73186     2262 RLlacneglqteglttVVVGVSQD--GIGIRCYTTTrEGISWALeeyshalqreptevpplHVIPARVTLSTELQGFFLV 2339 Trypanosoma b...
XP_001712807 2133 KLitnidglngnksifVILFVSNS--YSTLKAFSLTaEGFRNSLstniekl---dkdnikkYLRKVSISLSNKFQGFFLV 2207 Bigelowiella ...
XP_653245    2136 LIqehyn----idgqcICIGIGEG--AIGIEGYSFNeIGREFGKqnsdslit-lqnncteeLGVKQPLIVSEKINGFFLI 2208 Entamoeba his...
XP_001469202 2278 RAlianegmvpqgfvtAVLEMSEE--GVMARCYSTTaDGIAWAQseherllkrtpeatdpsFSSPCRGTLSSEARSFFLV 2355 Leishmania in...
2P87_A        211 PsNGVWNYNFQGqrwspamkFDVClSNPKEYYHEDHRPVHFHNF 254  nematode
NP_983156    2341 PkSGIWNYAFMGagfnrelrYELSlDIPLGFYDEQHRATHFLQF 2384 Ashbya gossypii ATCC 10895
XP_451233    2350 PsSSIWNYAFMGtafsedlpYELKlDIPLEFYNELHRAPHFIQF 2393 Kluyveromyces lactis NRRL Y-1140
XP_954981    2727 PvDGAWNYNFMGakhsphmnFQLQiEVPKPFYDPIHRPLHFIQF 2770 Theileria annulata strain Ankara
XP_447350    2353 PsTGVWNYYFMPtnlp-dtlDDMSiRIPVEFYNDIHRPNHFLQF 2395 Candida glabrata CBS 138
XP_001610701 2285 PtDDAWNYNFMGakhslhmqYQLQvEVPRPFYDPVHRPLHFIQF 2328 Babesia bovis T2Bo
CAA73186     2340 PtDNGWNHTFRGatwredttFDVRvDTPQFFFFATHRPDHFLNF 2383 Trypanosoma brucei brucei
XP_001712807 2208 PkNNLWNYHFFEsafnaemnYTLKvGIPIDYFEDYHRPFHFRDF 2251 Bigelowiella natans
XP_653245    2209 PeKGSWNYSLSTskftvnieYTLKaGIPLEFYNEAHRITHFLCW 2252 Entamoeba histolytica HM-1:IMSS
XP_001469202 2356 PaDRVWNYFFKGalwrenteYDVVvDVPLPFFHALHRPDHFLNF 2399 Leishmania infantum JPCM5
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