4OVW,1Q9H,2OVW,1EG1,1DYM,1H46,1CEL,1EGN,2RFW,2RG0


Conserved Protein Domain Family
GH7_CBH_EG

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cd07999: GH7_CBH_EG 
Click on image for an interactive view with Cn3D
Glycosyl hydrolase family 7
Glycosyl hydrolase family 7 contains eukaryotic endoglucanases (EGs) and cellobiohydrolases (CBHs) that hydrolyze glycosidic bonds using a double-displacement mechanism. This leads to a net retention of the conformation at the anomeric carbon. Both enzymes work synergistically in the degradation of cellulose,which is the main component of plant cell wall, and is composed of beta-1,4 linked glycosyl units. EG cleaves the beta-1,4 linkages of cellulose and CBH cleaves off cellobiose disaccharide units from the reducing end of the chain. In general, the O-glycosyl hydrolases are a widespread group of enzymes that hydrolyze the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycoside hydrolase family 7.
Statistics
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PSSM-Id: 153432
View PSSM: cd07999
Aligned: 18 rows
Threshold Bit Score: 417.211
Threshold Setting Gi: 16117340
Created: 30-Sep-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
active site
Conserved site includes 16 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:2RG0; Melanocarpus albomyces Cellobiohydrolase with bound cellobiose
    View structure with Cn3D
  • Structure:4OVW; Fusarium oxysporum endoglucanase I with bound 3,4-epoxybutyl beta-D-cellobioside-inhibitor; contacts 4A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                   # ##                                                 
4OVW_A         7 KEQHPKLETYRCTKaSGCKKQTnYIVADAGIHGIRQKNgagcgdwgqkpna----tacpdeascakncILSGMdsnaykn 82  Fusarium oxysporum
2OVW_A         7 KEQHPKLETYRCTKaSGCKKQTnYIVADAGIHGIRQKNgagcgdwgqkpna----tacpdeascakncILSGMdsnaykn 82  Fusarium oxysporum
P07981        30 PEVHPKLTTYKCTKsGGCVAQDtSVVLDWNYRWMHDANynsctvnggvntt-----lcpdeatcgkncFIEGVdya---a 101 Hypocrea jecorina
XP_370166     31 PEIHPELPTQFCTRkDGCVVRKtKIVADVMFRPMHVKDnvsmpctaafgta-----lcpdaatctrncVLEGVdyd---s 102 Magnaporthe gri...
XP_001906344  30 PELHPRFPTQQCTSsGICTTKQtSLVTDALSRHFHSVSdpsvscasvdflsnpslcdpddpascaancALEGIeyg---g 106 Podospora anser...
1EG1_A         8 PEVHPKLTTYKCTKsGGCVAQDtSVVLDWNYRWMHDANynsctvnggvntt-----lcpdeatcgkncFIEGVdya---a 79  Hypocrea jecorina
1DYM_A         7 KEVHPQLTTFRCTKrGGCKPATnFIVLDSLSHPIHRAEglgpggcgdwgnppp-kdvcpdvescakncIMEGIpdy--sq 83  Humicola insolens
XP_647587     27 PEVHPPMTFQKCSEgGSCETIQgEVVVDANWRWVHSAQgqncytgntwnpt-----icpddetcaencYLDGAnye--sv 99  Dictyostelium d...
BAB69425      20 AENHPSLSWQNCRSgGSCTQTSgSVVLDSNWRWTHDSSltncydgnewsss-----lcpdpktcsdncLIDGAdys--gt 92  Pseudotrichonym...
2RFW_A         8 PENHPPLTWQRCTApGNCQTVNaEVVIDANWRWLHDDNmqncydgnqwtn------acstatdcaekcMIEGAgdyl-gt 80  Melanocarpus al...
Feature 1                                    #                                  #   #            
4OVW_A        83 aGITTSgnKLRLQQLinn-----qlVSPRVYLLeenkkkYEMLHLTgTEFSFDVEmEKLPCGMNGALYLSEMPqdggkst 157 Fusarium oxysporum
2OVW_A        83 aGITTSgnKLRLQQLinn-----qlVSPRVYLLeenkkkYEMLHLTgTEFSFDVEmEKLPCGMNGALYLSEMPqdggkst 157 Fusarium oxysporum
P07981       102 sGVTTSgsSLTMNQYmpsssggyssVSPRLYLLdsd-geYVMLKLNgQELSFDVDlSALPCGENGSLYLSQMDengga-- 178 Hypocrea jecorina
XP_370166    103 lGVATKgnALKLNQYlfnga-eykkVAPRVYLLgeddrnYELMKLVnMELAFDVDvSQLGCGMNGALYFSEMDvsgsr-- 179 Magnaporthe gri...
XP_001906344 107 iGVSAVgsAVTLRQYlfdga-eyraVSPRVYLLaedgenYEPLTLLgQELAVDVDvSGLPCGMNSAVYLSEMDlsgsr-- 183 Podospora anser...
1EG1_A        80 sGVTTSgsSLTMNQYmpsssggyssVSPRLYLLdsd-geYVMLKLNgQELSFDVDlSALPCGENGSLYLSQMDengga-- 156 Hypocrea jecorina
1DYM_A        84 yGVTTNgtSLRLQHIlpd----grvPSPRVYLLdktkrrYEMLHLTgFEFTFDVDaTKLPCGMNSALYLSEMHptgak-- 157 Humicola insolens
XP_647587    100 yGVTTSedSVRLNFVtqsq---gknIGSRLFLMsne-snYQLFHVLgQEFTFDVDvSNLDCGLNGALYLVSMDsdggsar 175 Dictyostelium d...
BAB69425      93 yGITSSgnSLKLVFVtngp--ystnIGSRVYLLkde-shYQIFDLKnKEFTFTVDdSNLDCGLNGALYFVSMDedggtsr 169 Pseudotrichonym...
2RFW_A        81 yGASTSgdALTLKFVtkhe--ygtnVGSRFYLMngp-dkYQMFNLMgNELAFDVDlSTVECGINSALYFVAMEedggmas 157 Melanocarpus al...
Feature 1                     # # #                                    # #  #          #         
4OVW_A       158 srnskAGAYYGAGYCDAQCyvtpfingvg---------------nikgqgvccnELDIWEANSrATHIAPHPCskpglyg 222 Fusarium oxysporum
2OVW_A       158 srnskAGAYYGAGYCDAQCyvtpfingvg---------------nikgqgvccnELDIWEANSrATHIAPHPCskpglyg 222 Fusarium oxysporum
P07981       179 nqyntAGANYGSGYCDAQCpvqtwrngtl---------------ntshqgfccnEMDILEGNSrANALTPHSCta----- 238 Hypocrea jecorina
XP_370166    180 sdlnpAGAAYGTGECDAQCf----------------------------------QDQNFTANRmATALTPHTCskpssfr 225 Magnaporthe gri...
XP_001906344 184 sesnpAGAGYGTGYCDAQCfrtapwinglp--------------nlnssgaccnEMDIWEANSrANSFTPHTCsspgsfl 249 Podospora anser...
1EG1_A       157 nqyntAGANYGSGYCDAQCpvqtwrngtl---------------ntshqgfccnEMDILEGNSrANALTPHSCta----- 216 Hypocrea jecorina
1DYM_A       158 skynpGGAYYGTGYCDAQCfvtpfinglg---------------niegkgsccnAMDIWEANSrASHVAPHTCnkkglyl 222 Humicola insolens
XP_647587    176 fptneAGAKYGTGYCDAQCprdlkfisgsanvdgwipstnnpntgygnlgsccaEMDLWEANNmATAVTPHPCdtssqsv 255 Dictyostelium d...
BAB69425     170 fssnkAGAKYGTGYCDAQCphdikfingeanvenwkpqtndenagngrygacctEMDIWEANKyATAYTPHICtvngeyr 249 Pseudotrichonym...
2RFW_A       158 ypsnqAGARYGTGYCDAQCardlkfvggkaniegwksstsdpnagvgpygsccaEIDVWESNAyAFAFTPHACttneyhv 237 Melanocarpus al...
Feature 1                                                                                        
4OVW_A       223 ct-------gdecgssgiCDKAGCGWNHNRinvTDFYGrgkqykVDSTRKFTVTSQFvankq---------gdliELHRH 286 Fusarium oxysporum
2OVW_A       223 ct-------gdecgssgiCDKAGCGWNHNRinvTDFYGrgkqykVDSTRKFTVTSQFvankq---------gdliELHRH 286 Fusarium oxysporum
P07981       239 ----------------taCDSAGCGFNPYGsgyKSYYGpg--dtVDTSKTFTIITQFntdngs------psgnlvSITRK 294 Hypocrea jecorina
XP_370166    226 ctg-----eecgkgkagvCDKSGCQINPYKlgdKKFYGpg--gtIDTNKRFTVLTQFvhehpeagidisragplkEIRRK 298 Magnaporthe gri...
XP_001906344 250 csse----aecgkgapgvCDKDGCGLNTFNlgsPSFYGlg--ldIDSSNPFTIVTQFltnda---------gelsEIKRL 314 Podospora anser...
1EG1_A       217 ----------------taCDSAGCGFNPYGsgyKSYYGpg--dtVDTSKTFTIITQFntdngs------psgnlvSITRK 272 Hypocrea jecorina
1DYM_A       223 ce-------geecafegvCDKNGCGWNNYRvnvTDYYGrgeefkVNTLKPFTVVTQFlanrr---------gkleKIHRF 286 Humicola insolens
XP_647587    256 cksdscggaassnryggiCDPDGCDYNPYRmgnTSFFGpn--kmIDTNSVITVVTQFitddgs------sdgkltSIKRL 327 Dictyostelium d...
BAB69425     250 cdgsecgdtdsgnryggvCDKDGCDFNSYRmgnTSFWGpg--liIDTGKPVTVVTQFvtkdgt------dngqlsEIRRK 321 Pseudotrichonym...
2RFW_A       238 cettncggtysedrfagkCDANGCDYNPYRmgnPDFYGkg--ktLDTSRKFTVVSRFee---------------nKLSQY 300 Melanocarpus al...
Feature 1                                                                           # #        # 
4OVW_A       287 YIQDnKVIESAvvnisg----ppkinFINDkyCAatg-----aneYMRLGGTKQMGDAMSrGMVLAMSVWWSegDFMAWL 357 Fusarium oxysporum
2OVW_A       287 YIQDnKVIESAvvnisg----ppkinFINDkyCAatg-----aneYMRLGGTKQMGDAMSrGMVLAMSVWWSegDFMAWL 357 Fusarium oxysporum
P07981       295 YQQNgVDIPSAqpg----------gdTISS--CPs----------ASAYGGLATMGKALSsGMVLVFSIWNDnsQYMNWL 352 Hypocrea jecorina
XP_370166    299 YIQDgRVIEDVhtsnas----fsplkGMDQrfCDtyg-----seaYTRLGGIVGTGQSMArGMVLIFSIWNSegDFMKWL 369 Magnaporthe gri...
XP_001906344 315 YIQNgQVTPNTaettdsrfdgisyegSITEdfCAakn-----ssdYLRLGGMKGMGEALArGMVLVFSLWNSegDFMSWL 389 Podospora anser...
1EG1_A       273 YQQNgVDIPSAqpg----------gdTISS--CPs----------ASAYGGLATMGKALSsGMVLVFSIWNDnsQYMNWL 330 Hypocrea jecorina
1DYM_A       287 YVQDgKVIESFytnkeg----vpytnMIDDefCEatg-----srkYMELGATQGMGEALTrGMVLAMSIWWDqgGNMEWL 357 Humicola insolens
XP_647587    328 YVQDgNVISQSvstid-----gvegnEVNEefCTnqkkvfgdedsFTKHGGLAKMGEALKdGMVLVLSLWDDyqANMLWL 402 Dictyostelium d...
BAB69425     322 YVQGgKVIENTvvniag----mssgnSITDdfCNeqksafgdtndFEKKGGLSGLGKAFDyGMVLVLSLWDDhqVNMLWL 397 Pseudotrichonym...
2RFW_A       301 FIQDgRKIEIPpptweg----mpnssEITPelCStmfdvfndrnrFEEVGGFEQLNNALRvPMVLVMSIWDDhyANMLWL 376 Melanocarpus al...
Feature 1                                                       
4OVW_A       358 Dqg------------vAGPCDATegdpknivkvqPNPEVTFSNIRIG 392 Fusarium oxysporum
2OVW_A       358 Dqg------------vAGPCDATegdpknivkvqPNPEVTFSNIRIG 392 Fusarium oxysporum
P07981       353 Dsg------------nAGPCSSTegnpsnilannPNTHVVFSNIRWG 387 Hypocrea jecorina
XP_370166    370 Dag------------dRGPCGPTdgdpkkikqetPNVSVTYSNIKWG 404 Magnaporthe grisea 70-15
XP_001906344 390 Dgl-----------psNGPCNATegdpaliraqvPDVSVTFSNVRWG 425 Podospora anserina DSM 980
1EG1_A       331 Dsg------------nAGPCSSTegnpsnilannPNTHVVFSNIRWG 365 Hypocrea jecorina
1DYM_A       358 Dhg------------eAGPCAKGegapsnivqvePFPEVTYTNLRWG 392 Humicola insolens
XP_647587    403 DssypttssptdpgvaRGSCPTTsgvpskveqnyPNAYVVYSNIKVG 449 Dictyostelium discoideum AX4
BAB69425     398 DsiyptdqpasqpgvkRGPCATSsgapsdvesqhPDSSVTFSDIRFG 444 Pseudotrichonympha grassii
2RFW_A       377 Dsiyppek-egqpgaaRGDCPTDsgvpaeveaqfPDAQVVWSNIRFG 422 Melanocarpus albomyces

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