1IG3,2F17,3CQ9,3IHK,2HH9,1IG0,2OMK


Conserved Protein Domain Family
TPK

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cd07995: TPK 
Click on image for an interactive view with Cn3D
Thiamine pyrophosphokinase
Thiamine pyrophosphokinase (TPK, EC:2.7.6.2, also spelled thiamin pyrophosphokinase) catalyzes the transfer of a pyrophosphate group from ATP to vitamin B1 (thiamine) to form the coenzyme thiamine pyrophosphate (TPP). TPP is required for central metabolic functions, and thiamine deficiency is associated with potentially fatal human diseases. The structure of thiamine pyrophosphokinase suggests that the enzyme may operate by a mechanism of pyrophosphoryl transfer similar to those described for pyrophosphokinases functioning in nucleotide biosynthesis.
Statistics
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PSSM-Id: 153431
View PSSM: cd07995
Aligned: 265 rows
Threshold Bit Score: 106.86
Threshold Setting Gi: 253741776
Created: 1-Oct-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 27 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:The active site is located at the dimer interface, with residues from both monomers participating
  • Structure:2F17: Mouse TPK dimer binds AMP (or EPE), Mg, and the antibacterial compound pyrithiamin pyrophosphate, a substrate analog, at each of the two binding sites; contacts at 4A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1            ###                      ###                       # ##                     
1IG3_A        40 CLVVLNQPldarfr----hlwkkaLLRACADGGANHLYDltegereSFLPEFVSGDFDSIr---PEVKEYYtkk-----g 107 house mouse
2F17_A        40 CLVVLNQPldarfr----hlwkkaLLRACADGGANHLYDltegereSFLPEFVSGDFDSIr---PEVKEYYtkk-----g 107 house mouse
ZP_03946731  245 VLIVAGSPapsslklv-qklarvnDYLIAADGGANVLYEa------GIVPQVFCGDADSVn---GDAYKWVtsc-----v 309 Atopobium vagin...
ZP_05798607   22 VLLIAGGKppsdawl---eattrcFDVWVADRGVTYCRKl------NVIPKAIVGDGDSSp---LEDWAWAeea-----g 84  Anaerobaculum s...
ZP_03567651  239 SVLVVDGSptpssaalvselsscsDYVVAADRGAYICKEa------GVVPDIACGDFDSVg---EETLSWIhaq-----k 304 Atopobium rimae...
ZP_05405112   37 CLLVVGGRapeaawl---reaakgREVWAVDHGLDACLAa------GIRPQRLIGDGDSAa---PAAWQLAkeq-----h 99  Mitsuokella mul...
YP_003179884 235 EVLIVGGSpeavskttlstcvqsaDYLIAVDHGADACHVa------GVVPQLALGDFDSAs---LETVTWLkeq-----q 300 Atopobium parvu...
ZP_05898377   24 CTLVTGGRppaadw----lqrarrGKLWVIDHGVDLCHAm------KCVPDFLLGDADSAs---PAAWSWAlae-----g 85  Selenomonas spu...
ZP_03928922   20 AGLVAGGRgpdpnwl---laamrdLPLYGADKGVEYCVRa------GLYPVLACGDRDSGh---AGSWEKIkah-----g 82  Acidaminococcus...
ZP_03297739   16 YTLVVGGSpvvldaaalallargcPRVVAVDRGLDALIDa------GVTCDLFCGDADSVgargQSLVDRAltgdsgfir 89  Collinsella ste...
Feature 1                 ######                         # ###  #   #                            
1IG3_A       108 cdLISTPd-QDHTDFTKCLQVLQRkieekelqvdvIVTLGGLGGRFDQIMASVNTLfqathitpvpIIIIQk--dSLIYL 184 house mouse
2F17_A       108 cdLISTPd-QDHTDFTKCLQVLQRkieekelqvdvIVTLGGLGGRFDQIMASVNTLfqathitpvpIIIIQk--dSLIYL 184 house mouse
ZP_03946731  310 ekKITFPreKYATDLQLALDCAVHearrr-rkllhVTLTCASGGRADHALGVVGVLaraa--sycpTIVEDsytlQIISL 386 Atopobium vagin...
ZP_05798607   85 akVYRYPkdKDYTDLQLAIMQISRsygr----svsVFVTAGLGGRLDHSLSNIFSLrlaekwgvdaAGFLDe--eESLIL 158 Anaerobaculum s...
ZP_03567651  305 vhTIAYPqdKYETDLSLALNAACHeatrq-alplsLTLTCASGGRLDHELGVVGLLarlst-aswrVRIVEd--aFEARI 380 Atopobium rimae...
ZP_05405112  100 ipIEQFPveKDDTDTQLALKLAREagf------paAIVTGAFGGRFDHALSTVTSCafa----plpCLLADe--rESLAF 167 Mitsuokella mul...
YP_003179884 301 vpCMKFNadKYDTDLALALKSAEHeairr-nsklsLTVVSTSGGHLDHQLVVLGLLaawaktgkakVRVVEn--dFEMRF 377 Atopobium parvu...
ZP_05898377   86 vpCERFDvkKDLTDTQLALVKIKEkap-----dtfLLLTGAFGGRFDHAMSTLFSCafs----gipMILADe--qEACFF 154 Selenomonas spu...
ZP_03928922   83 tkVMTYLvnKDATDLSLLLKEMPPvg--------tLLASGVWGGRADHLYANFLTLlqyqkrtktpVVLLDe--wEMLVF 152 Acidaminococcus...
ZP_03297739   90 dvERYDPr-KDFTDLALALRAVRErwg-----dvpLLCTSLSGGRTDHELAALGCLlsw----rgrIAWEEp--sFSGRL 157 Collinsella ste...
Feature 1                                                   # #           ####                   
1IG3_A       185 Lq---pGKHRLHvdtg---megSWCGLIPVGqpcnqVTTTGLKWNLTNdvlgfgtLVSTSNTYdgs-glvTVETD----H 253 house mouse
2F17_A       185 Lq---pGKHRLHvdtg---megSWCGLIPVGqpcnqVTTTGLKWNLTNdvlgfgtLVSTSNTYdgs-glvTVETD----H 253 house mouse
ZP_03946731  387 R-----GRSHIHfaqt---dikKTVSVIALTpw-tcISETGFKWNLSHydlaqfsDEGISNVVeds--gaTIVCHs---G 452 Atopobium vagin...
ZP_05798607  159 Lr----GGEELKlhfynkplaiSLLSLTPCSh---gVSIKKVKWELDNatlrldePFAVSNELind--erEVDLKvd-sG 228 Anaerobaculum s...
ZP_03567651  381 LsadtyAAWRLSekd-----rgKTLSVLPLQee-tvITEIGMQWDLASrtllllsDEGISNVVqtd--vaQIHCEk---G 449 Atopobium rimae...
ZP_05405112  168 Vr----GGETLScrpa---kapKAISLLPLAprcegVNLTGTHWPLTDatlearnMRAISNVLepgstslTLSLAsgllG 240 Mitsuokella mul...
YP_003179884 378 LaadqiDSWQLDasa-----tgKKISLVALSee-ceVSESGMRWNLNHekftllgDDGISNIVead--gaWVKCEk---G 446 Atopobium parvu...
ZP_05898377  155 Lh----EEDSLSftak---qtpKAISLLALGgecrgVSLAGTQWPLEGavlrqeePYAVSNELaegsssfRASLQa---G 224 Selenomonas spu...
ZP_03928922  153 Lek--gDRLSFEaqk-----lpKALSLLPFSpv-ceVSIDGVRWPLEHdvissenPYAVSNKMagr--ktQASCHagiaG 222 Acidaminococcus...
ZP_03297739  158 Lrs--gDAWDLTgl------agSTFSFVCLSpe-asLSERGMAWPLDHrrvrlleDLGISNVLrd---daVIACHe---G 222 Collinsella ste...
Feature 1               
1IG3_A       254 PLLWTMA 260 house mouse
2F17_A       254 PLLWTMA 260 house mouse
ZP_03946731  453 KAAVFIM 459 Atopobium vaginae DSM 15829
ZP_05798607  229 CVGVYIC 235 Anaerobaculum sp. OS1
ZP_03567651  450 KALVVLL 456 Atopobium rimae ATCC 49626
ZP_05405112  241 LYFVWRE 247 Mitsuokella multacida DSM 20544
YP_003179884 447 CLLVQLW 453 Atopobium parvulum DSM 20469
ZP_05898377  225 TLAVYLY 231 Selenomonas sputigena ATCC 35185
ZP_03928922  223 FYLTYKE 229 Acidaminococcus sp. D21
ZP_03297739  223 SVMAFCL 229 Collinsella stercoris DSM 13279

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