Conserved Protein Domain Family
DRE_TIM_HCS

?
cd07948: DRE_TIM_HCS 
Saccharomyces cerevisiae homocitrate synthase and related proteins, catalytic TIM barrel domain
Homocitrate synthase (HCS) catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, the first step in the lysine biosynthesis pathway. This family includes the Yarrowia lipolytica LYS1 protein as well as the Saccharomyces cerevisiae LYS20 and LYS21 proteins. This family belongs to the DRE-TIM metallolyase superfamily. DRE-TIM metallolyases include 2-isopropylmalate synthase (IPMS), alpha-isopropylmalate synthase (LeuA), 3-hydroxy-3-methylglutaryl-CoA lyase, homocitrate synthase, citramalate synthase, 4-hydroxy-2-oxovalerate aldolase, re-citrate synthase, transcarboxylase 5S, pyruvate carboxylase, AksA, and FrbC. These members all share a conserved triose-phosphate isomerase (TIM) barrel domain consisting of a core beta(8)-alpha(8) motif with the eight parallel beta strands forming an enclosed barrel surrounded by eight alpha helices. The domain has a catalytic center containing a divalent cation-binding site formed by a cluster of invariant residues that cap the core of the barrel. In addition, the catalytic site includes three invariant residues - an aspartate (D), an arginine (R), and a glutamate (E) - which is the basis for the domain name "DRE-TIM".
Statistics
?
PSSM-Id: 163685
View PSSM: cd07948
Aligned: 18 rows
Threshold Bit Score: 361.264
Threshold Setting Gi: 149917685
Created: 17-Sep-2009
Updated: 2-Oct-2020
Structure
?
Aligned Rows:
 
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the active sites of other DRE-TIM superfamily members such as 3-hydroxy-3-methylglutaryl-CoA lyase.

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1                ##  #                          #                             # #        
CAA98757      23 FQLIDSTLREGEQFANAFFDTEKKIEIARALDDFGVDYIELTSPVASEQSRKDCEAICKLGLk-aKILTHIRCHMDDAKV 101 baker's yeast
YP_001633968   7 LFFVDTTLREGEQFASARFTTAQRIAIAEMLDAFGVEYIELTSPAASPQSARDLATIARRGLr-aRILTHIRCHMADARL 85  Chloroflexus au...
YP_001546065   5 FAIVDTTLREGEQWANAHFSSEDRLAIANLLVQFGVEYIEMTSPCASPQSTADLRSIAALPLgntKLLTHTRCNLDDVRL 84  Herpetosiphon a...
XP_001749906  38 FEIIDTTLREGEQFSSTDFTSHDRVYIAKLLDTFGVDYIELVNPFASHQAREDCRAIANLGLk-aKIVAHTRCHMDDVRA 116 Monosiga brevic...
NP_294962     19 WAIIDSTLREGEQFARGNFGTDDKVEIARALDAFGAEYIEVTTPMVSEQTRQDIRKLTGLGLr-aKFLTHVRCHMEDVQR 97  Deinococcus rad...
ZP_03858077    6 FYIVDSTLREGEQFVHAHFTTEQKIEIARALDAFGVEYIELTNPAASPASFEDARRIANLGLr-aRILVHCRCTMADVQQ 84  Thermobaculum t...
YP_002376271   6 FSIIDSTLREGEQFYGANFSSEDRLEIAQSLNNFGVEYIEVTSPCVSPQSRQDCQQIARLGLh-sKILTHIRCNLEDAKI 84  Cyanothece sp. ...
BAA33785       4 WKIIDSTLREGEQFEKANFSTQDKVEIAKALDEFGIEYIEVTTPVASPQSRKDAEVLASLGLk-aKVVTHIQCRLDAAKV 82  Thermus thermop...
CAX65450      68 FKIIESTLREGEQFANAYFSTETKIKIAKMLDAFGVDYLELTNPAASPQSRDDCEAICKLGLk-aKILTHTRCTLEDAKL 146 Gibberella fuji...
YP_001379579   6 WKLIDTTLREGEQFAKSSFRTEDKLEIARALDTFGVDYIEVTTPAASPQSQRDLAQIVKLGLa-aRIITHTRCILDDVKA 84  Anaeromyxobacte...
Feature 1                                                                #                       
CAA98757     102 AVETGVDGVDVVIGTSKFLRqy-sHGKDMNYIAKSAVEVIEFVKSKg--iEIRFSSEDSFRSDLVDLLNIYKTVDki-gV 177 baker's yeast
YP_001633968  86 AVEHGAQGANLLFATSEPLRtv-sHGRSLSEILAEAKRVICYLRDHd--iEVRFSCEDSFRTDLADLIRIYRAVEtm-gV 161 Chloroflexus au...
YP_001546065  85 AAECGVAGVNILFGTSPYLRqw-sHGRSIEAILEEVGPVVRFLQERn--iEVRFSCEDSFRTPLPDLLRVYQAVDll-gV 160 Herpetosiphon a...
XP_001749906 117 CVETGIDGVSMYMATSEALRrh-sHGKSVQAVIDSAREVIEFVKDNg--mEVRFSCEDTFRSDRTELLDIYAAIDel-gV 192 Monosiga brevic...
NP_294962     98 AVDTGVDGLDLLFGTSSFLRef-sHGKSIAQIIDTAGEVIGWIKTHhpelEIRFSAEDTFRSEEADLMAVYSAVSel-gV 175 Deinococcus rad...
ZP_03858077   85 AVETGVDGVNLLFATSEQLRth-sHGKSIDEIIESARGVISFLQDHg--lEVRFSCEDAFRTPIDILLRVYAAVDam-gV 160 Thermobaculum t...
YP_002376271  85 AVDTGVDGINLFFGTSSLLRqf-gHGKSIPQILEIATEVITFIHEQspatELRFSTEDSFRSELSDLLRVYLTIDslgiI 163 Cyanothece sp. ...
BAA33785      83 AVETGVQGIDLLFGTSKYLRa--pHGRDIPRIIEEAKEVIAYIREAaphvEVRFSAEDTFRSEEQDLLAVYEAVAp--yV 158 Thermus thermop...
CAX65450     147 ACDCGVDGVDVVIGTSQFLRehslGGKNMTAITKQAIEVIEYIKSRg--cEVRFSSEDSFRSDLVDLLSIYRAVNnv-gV 223 Gibberella fuji...
YP_001379579  85 ALDAGVRGIGIFFATSRILRea-sHAKSLQQIVDAMGPPIEAALAAg--lEVRFSAEDAFRSDVHDLLAVYRAAEkl-gV 160 Anaeromyxobacte...
Feature 1             #                              # #                                         
CAA98757     178 NRVGIADTVGCANPRQVYELIRTLKSVvs--cDIECHFHNDTGCAIANAYTALEGGARLIDVSVLGIGERNGITPLGGLM 255 baker's yeast
YP_001633968 162 QRVGLADTVGIATPRQVYDVVRAVRNEvq--cDIEFHGHNDSGCAIANTFSAYEAGATHLDVTVLGIGERNGIASLSGMI 239 Chloroflexus au...
YP_001546065 161 QRVGIADTVGIATPRDVERVVGAVRNAvr--cDIEFHGHNDGGCAIANAYAALEAGATHIDTTILGIGERNGIAALSGLI 238 Herpetosiphon a...
XP_001749906 193 NRVGLADTVGIATPFQVAETVAAVRSIikpetGIEFHTHNDTGCCIANALVALQAGATHINTCVLGVGERNGITPLGGFL 272 Monosiga brevic...
NP_294962    176 HRVGLADTVGVATPRQVYTLVREVRKVihegcGIEFHGHNDTGCAVSNAYEAIEAGATHIDTTILGIGERNGITPLGGLL 255 Deinococcus rad...
ZP_03858077  161 DRVGIADTVGVADPLGVHELVSRVRGVvs--aDIEFHGHNDSGCAIANAYAALQAGATHIDTTVLGIGERNGITPLGGFI 238 Thermobaculum t...
YP_002376271 164 KRFGIADTVGIATPNQVFNLVQILRQStd--kDIEFHAHNDTGCAIANSYSALEAGATHIDTTVLGIGERNGITPLAGLI 241 Cyanothece sp. ...
BAA33785     159 DRVGLADTVGVATPRQVYALVREVRRVvgprvDIEFHGHNDTGCAIANAYEAIEAGATHVDTTILGIGERNGITPLGGFL 238 Thermus thermop...
CAX65450     224 NRVGVADTVGLASPRQVYDLVRTLRGCvs--tDIEVHFHDDTGCAVANAFTALEAGATHVDTSVLGLGERNGITPLGALM 301 Gibberella fuji...
YP_001379579 161 HRVGVADTVGIATPRQVYALFREVRSAvk--cDLGFHGHNDTGCAIANAYEAISAGATHVDVSVLGIGERNGITPLGGIV 238 Anaeromyxobacte...
Feature 1                                     
CAA98757     256 ARMIvaapdyvkSKYKLHKIRDIENLVAD 284 baker's yeast
YP_001633968 240 ARIAsidp-srvQRYRLDLLPKIDETVAT 267 Chloroflexus aurantiacus J-10-fl
YP_001546065 239 ARLYlsep-esvAGYALPMLAQLDHTVAA 266 Herpetosiphon aurantiacus ATCC 23779
XP_001749906 273 ARMYtldresvkNKYDLKLLRHLEKYVAA 301 Monosiga brevicollis MX1
NP_294962    256 ARMFtfdpqgliDKYNLELLPELDRMIAR 284 Deinococcus radiodurans R1
ZP_03858077  239 ARLYatdr-glvSRYNLRMLPQLDSLISQ 266 Thermobaculum terrenum ATCC BAA-798
YP_002376271 242 ARLYtldpetlsRKYHLHELLNLHQLVAN 270 Cyanothece sp. PCC 7424
BAA33785     239 ARMYtlqpeyvrRKYKLEMLPELDRMVAR 267 Thermus thermophilus HB27
CAX65450     302 ARMMptsrdyilSKYKLQELKKIEDFVAE 330 Gibberella fujikuroi
YP_001379579 239 ARMFalepqavaERYRLGQLRELERLVAR 267 Anaeromyxobacter sp. Fw109-5

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap