Conserved Protein Domain Family
DRE_TIM_LeuA3

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cd07941: DRE_TIM_LeuA3 
Desulfobacterium autotrophicum LeuA3 and related proteins, N-terminal catalytic TIM barrel domain
Desulfobacterium autotrophicum LeuA3 is sequence-similar to alpha-isopropylmalate synthase (LeuA) but its exact function is unknown. Members of this family have an N-terminal TIM barrel domain that belongs to the DRE-TIM metallolyase superfamily. DRE-TIM metallolyases include 2-isopropylmalate synthase (IPMS), alpha-isopropylmalate synthase (LeuA), 3-hydroxy-3-methylglutaryl-CoA lyase, homocitrate synthase, citramalate synthase, 4-hydroxy-2-oxovalerate aldolase, re-citrate synthase, transcarboxylase 5S, pyruvate carboxylase, AksA, and FrbC. These members all share a conserved triose-phosphate isomerase (TIM) barrel domain consisting of a core beta(8)-alpha(8) motif with the eight parallel beta strands forming an enclosed barrel surrounded by eight alpha helices. The domain has a catalytic center containing a divalent cation-binding site formed by a cluster of invariant residues that cap the core of the barrel. In addition, the catalytic site includes three invariant residues - an aspartate (D), an arginine (R), and a glutamate (E) - which is the basis for the domain name "DRE-TIM".
Statistics
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PSSM-Id: 163679
View PSSM: cd07941
Aligned: 86 rows
Threshold Bit Score: 401.062
Threshold Setting Gi: 227495707
Created: 14-Sep-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the active sites of other DRE-TIM superfamily members such as 3-hydroxy-3-methylglutaryl-CoA lyase.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1              ##  #                          #                                   # #    
YP_002603359   6 IYDTTLRDGMQGENICFSPEEKVKIAQKLDDMGIHYIEGGWPGSNPSAVRFFDLIKDvkf------kcaKIAAFGSTRRA 79  Desulfobacteriu...
YP_003110134  11 IYDTTLRDGSQQEGISLSVDDKLKVARQLDALGVHYIEGGWPGANPKDAAFFARARDelal-----htaQLVAFGSTRRA 85  Acidimicrobium ...
ZP_03924037   10 IYDTTLRDGAQMEGISLSVADKLNIAQALDDLGVSFIEGGWPGAIPKDTEFFALALSklql-----rhaTLAAFGATRKV 84  Mobiluncus curt...
YP_003155281  16 LYDTTLRDGSQQEGLTLSVADKLAVARHLDELGVTYIEGGWPGAVPRDTEFFARAAGelel-----hhaRLAAFGATRKA 90  Brachybacterium...
ZP_03926018    9 TYDTTLRDGAQQQSVSFTVEDKLAVARILDHLGVTYIEAGWPGAIPKDTEFFARAASelsl-----ataRLVAFGSTCHP 83  Actinomyces uro...
XP_002508861  66 CYDTTLRDGSQQVGISLTCDDKLAVAEHLVGLGVAYIEGGYPGSNPKDVEFFSRWTSsgladraaaggtKLAAFGMTRRR 145 Micromonas sp. ...
YP_872471     19 VYDTTLRDGAQGEGMALTVADKLAIARHLDDLGVGFIEGGWPGALPKDTEFFRRARTeltl-----rnaVLVAFGATRKP 93  Acidothermus ce...
ZP_01452432   12 LYDTTLRDGAQSAGIHFSAEDKKRIARRLASFGMDWIEGGWPGASPTDDCFFELMRDraw------shsRLVAFGSTARP 85  Mariprofundus f...
ZP_04485404   11 VFDTTLRDGAQREGISYSVADKLAVTKLLDELGVGFIEGGWPGALPSDTEFFARAAEeltl-----khaTLVAFGATRKA 85  Stackebrandtia ...
NP_823886     13 VFDTTLRDGAQREGINLTVADKLAIARHLDDFGVGFIEGGWPGANPRDTEFFARAQQeidf-----khaQLVAFGATRRA 87  Streptomyces av...
Feature 1                                                                         #              
YP_002603359  80 NTRAREDRNLQALVKCGAPVVTIFGKSWNLHVEKIMNNTAEENLAMIEESIVFLIGEkrEVIYDAEHFFDGFIADPDYAI 159 Desulfobacteriu...
YP_003110134  86 STTAESDPNLAALTNAGVGLACIVAKAWDVHVIHALRTSLPEAVAMVADSVRWLVDHgiRVMLDAEHFFDGWRHDRSFAL 165 Acidimicrobium ...
ZP_03924037   85 GTKASEDPQVLALLESGAPVITLVAKSDLRHVTDALKTTGQENLAMVRDTVQFLVKEgrRVFLDAEHYFDGLTHDAEYGL 164 Mobiluncus curt...
YP_003155281  91 GVAVQEDPQVAALVDSGAPTITLVAKSDLRHVTGALRTTGEENLAMVGETVAHLVSLgrEVFVDAEHFFDGFLHDPDYTT 170 Brachybacterium...
ZP_03926018   84 GTAAEKDPQLRGLLDSGAPVITIVAKSDPAHVERALRTSGEENLRMVADSVGLLVAAgrEVMVDLEHYVDGVARDPRYGI 163 Actinomyces uro...
XP_002508861 146 GVTADDDEGLKALTDCPAPCVCIVAKAWDEQCEKVLGVSPEENIAMIEESVAHLVAAgkEVLVDCEHYFDGRKANPEFAV 225 Micromonas sp. ...
YP_872471     94 ESRVEADPQVLALLEAETPAVCLVAKSHVRHVSEALRTSLDENLAMIRDTVAFFRREgrRVFLDAEHFFDGYAADPQYAV 173 Acidothermus ce...
ZP_01452432   86 GHPASEDPGLARLVACGADAVCIFGKSWDLHVTTGLGIDLQTNLDLVRESVAWLKLHvdTVLFDAEHFFDGYAANRDYAM 165 Mariprofundus f...
ZP_04485404   86 GLAVEDDPQVAALLDSRASAWCLVAKSDVRHVKRALRTSPEENLAMVADTVAYGVSCgrRVFLDCEHFFDGFATDPDYAT 165 Stackebrandtia ...
NP_823886     88 GAKASEDPQVKALLEAGAQVITLVAKSHDRHVELALRTTLDENLEMVRDTVSYLCSQgrRVFVDCEHFFDGYRANPEYAK 167 Streptomyces av...
Feature 1                        #                                  # #                          
YP_002603359 160 ATLKAAIKGGCKTLVLCDTNGGTLPHELEKITRTVTATmeqtydfpvqIGIHTHNDIGMAVANAITAVRAGAGMVQGTIN 239 Desulfobacteriu...
YP_003110134 166 DVLAAAADAGAERLVLCDTNGGTLPDDVGPIVDDVRAAva------tpLGVHFHNDSGCAVANSLLAVAHGVIQVQGCMN 239 Acidimicrobium ...
ZP_03924037  165 KVLKAAGEAGAETVVLCDTNGGNLPSSIGAKVMEARDYldkngltqvkIGIHTHNDTGCAVANAMAAIEAGAAQVQGCVN 244 Mobiluncus curt...
YP_003155281 171 SVLLAAHRAGAGVLVLCDTNGGMLPHQVTEIIEDLRARlaragaadarLGAHTHNDTGCAVANALTAVRAGITHVQGCIN 250 Brachybacterium...
ZP_03926018  164 ELALTAARAGASAVIPCDTNGGSLPGTVAAEISRLRAAldaaghedcvVGIHTHDDSGLAVAAAMAAVEAGARQVQGCVN 243 Actinomyces uro...
XP_002508861 226 KCAAAAAKSGAKFVVLCDTNGGTLPWDVEEFTREVKEAvdatg-adcsVGIHCHNDTSLAVANSIAAVRGGATMVQGCVN 304 Micromonas sp. ...
YP_872471    174 ECVRVAAESGAEVVVLCDTNGGMLPPRIADVVHEVAERtg------vpLGIHCHDDTGCAVANTLAAVDAGVVQVQGVVN 247 Acidothermus ce...
ZP_01452432  166 QVLAAAADAGADSLVLCDTNGGSLPEQIGACVRQVVARypd-----ltIGIHAHNDSEMAVANAVSAVSAGASHVQGTIN 240 Mariprofundus f...
ZP_04485404  166 AVVRTALDAGADVVVLCDTNGGMLPSGISRTITELRERlgg----nprLGIHCQDDTACAVANTITAVEAGVMHVQGTAN 241 Stackebrandtia ...
NP_823886    168 AVVRAAAEAGADVVVLCDTNGGMLPAQVQAVVATVLADtg------arLGIHAQDDTGCAVANTLAAVDAGATHVQCTAN 241 Streptomyces av...
Feature 1                                                          
YP_002603359 240 GYGERCGNADLTSIIPILALKmds------pcVSPENLKKLLPLSRFVSE 283 Desulfobacterium autotrophicum HRM2
YP_003110134 240 GYGERTGNANLVPVIAGLSLKrsv------rtIPPENLELLTTVSRHIAE 283 Acidimicrobium ferrooxidans DSM 10331
ZP_03924037  245 GYGERTGNADILTLAGNIEIKlgr-----palLNEKGLAELTRTSHRVAE 289 Mobiluncus curtisii ATCC 43063
YP_003155281 251 GYGERTGNADLVTLLADLQLKmgm------elLPAETLSETTRIAHAIGE 294 Brachybacterium faecium DSM 4810
ZP_03926018  244 GFGERTGNANLLTLIANLELKtgyqalagdsqERARRLAELSAVSRTVGE 293 Actinomyces urogenitalis DSM 15434
XP_002508861 305 GYGERTGNADLLVVAANLELKmgr------taLPEGSLQRLTQVSAAVAK 348 Micromonas sp. RCC299
YP_872471    248 GYGERCGNANLITVVANLELKmgr------rvLPPGRLAELGRVSHAIAE 291 Acidothermus cellulolyticus 11B
ZP_01452432  241 GLGERCGNTNLISVIPVLKLKmgv-----ncgVSTESLRKLRRLSTFVNE 285 Mariprofundus ferrooxydans PV-1
ZP_04485404  242 GYGERPGNADLFAVVGNLATKmgm------dvLPDGGLAKLTHVSHAIAE 285 Stackebrandtia nassauensis DSM 44728
NP_823886    242 GYGERVGNSNLFPVVAALELKygk------kvLPEGALREMTRISHAIAE 285 Streptomyces avermitilis MA-4680

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