3B6R,1M15


Conserved Protein Domain Family
eukaryotic_phosphagen_kinases

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cd07931: eukaryotic_phosphagen_kinases 
Click on image for an interactive view with Cn3D
Phosphagen (guanidino) kinases mostly found in eukaryotes.
Phosphagen (guanidino) kinases are enzymes that transphosphorylate a high energy phosphoguanidino compound, like phosphocreatine (PCr) in the case of creatine kinase (CK) or phosphoarginine in the case of arginine kinase, which is used as an energy-storage and -transport metabolite, to ADP, thereby creating ATP. The substrate binding site is located in the cleft between the N and C-terminal domains, but most of the catalytic residues are found in the larger C-terminal domain. In higher eukaryotes, CK exists in tissue-specific (muscle, brain), as well as compartment-specific (mitochondrial and cytosolic) isoforms. They are either coupled to glycolysis (cytosolic form) or oxidative phosphorylation (mitochondrial form). Besides CK and AK, the most studied members of this family are also other phosphagen kinases with different substrate specificities, like glycocyamine kinase (GK), lombricine kinase (LK), taurocyamine kinase (TK) and hypotaurocyamine kinase (HTK).
Statistics
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PSSM-Id: 153078
View PSSM: cd07931
Aligned: 19 rows
Threshold Bit Score: 366.216
Threshold Setting Gi: 24658642
Created: 16-Sep-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 13 residues -Click on image for an interactive view with Cn3D
Feature 1:ADP binding site [chemical binding site]
Evidence:
  • Structure:1M15: a transition state analogue of Limulus polyphemus arginine kinase binds ADP-Mg++, contacts at 4A
    View structure with Cn3D
  • Citation:PMID 9041648

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                        
3B6R_B        23 LSAHNNHMAKVLT-PELYAELRAKSTP-SGFTLDDVIQTGVDNpghp----yimTVGCVAGDEESYEVFKDLFDPIIEDR 96  human
1M15_A        20 ASDCKSLLKKHLT-KDVFDSIKNKKTG-MGATLLDVIQSGVENld--------sGVGIYAPDAESYRTFGPLFDPIIDDY 89  Limulus polyphemus
NP_726252     75 QLSVTSYLSRFLK-RGVYDKIKRRQTR-LDHNLFDVLWPAMRKtskarhleediNCGIIAPDFDVFVVFQEFLVPLLKDM 152 fruit fly
XP_002293099  10 TPYHNSLLKKYLT-PSIWSHLRPPLQTpSGTTIEDVIRAGLALplga---npprRVGVLAGDQDCYEVFGELLDPIIWEY 85  Thalassiosira p...
XP_001641418  24 LSLNNTLMAKYLT-PEMYEKLKNRKTS-GKFTLEKLIQVGVDCpsvp----wgrAAGVVAGDEETYTVFSPILDSVIKDL 97  starlet sea ane...
ABN49967      35 LEVRESHPGNRMAkHFDVDYYLSLSDD-DKKALIKCCQSGIDNgd--------sGMGCYAMQPADYDRFKPFFSKVLADY 105 Monosiga brevic...
CAG35764      42 PENAAGLAKKYLS-PEILQALKGETTD-SGFTLAMAIRSGVLNpd--------sSIGIYAGDAQSYRTFAAILHPIIEEY 111 Desulfotalea ps...
ABH10974      33 EGGNMSLMCQTMT-KEMFEKLKGLKTSsGGWTVARAMNTGTLYpt--------sFVGCHAGDLESYSLYKDLFHPVIEAY 103 Suberites fuscus
XP_001450149  35 ATVKVSLGNKYFT-PEVVEQAKKLSAA-DQQRFLDVMMGGLANdd--------sSVGVYATRPEDYDVFSFYLQPLIREY 104 Paramecium tetr...
ABH10968       9 LEIKKTHPSNIMAaCFDASYYESLTPD-SQDRFLKCLNSGIENpd--------sQVGCYACQPDDYDAFRPFFLNVLQSY 79  Aphrocallistes ...
Feature 1                                                              # # #                     
3B6R_B        97 Hg-----gYKPSDEHk--TDLNpdnl---qggDDLDPn-------------yVLSSRVRTGRSIrGFCLPphcSRGERRA 153 human
1M15_A        90 Hg-----gFKLTDKHp-pKQWGdin-----tlVGLDPag-----------qfIISTRVRCGRSLqGYPFNpclTAEQYKE 147 Limulus polyphemus
NP_726252    153 Hc-----lSIDADFKp-qPRLAyf-------pMDNGErlntaafvfddesqlVTRCLVEVSRNLdQLELPlnlTIGQLEQ 219 fruit fly
XP_002293099  86 HgirqggvFEWEDPEl--PREGyf------gvEGGGEtkg----------kyIVSTRIRVARSLdGVRFPtmmSRSDRRR 147 Thalassiosira p...
XP_001641418  98 Hd------YGPEEKQk--RDVDckg-----lrDATIPra------------kWKATRITAWRSLkGYRFPaacGRLDRRQ 152 starlet sea ane...
ABN49967     106 Hk------VGEDAKHt--NNWDlsgveglpadGRLDLaal---------glpALSMRVRVGRNLaDFPLPgamTKDDRVN 168 Monosiga brevic...
CAG35764     112 Hg------VSGEVRQe--SDLAav------tlANLDPeg-----------ryIRSSRVRVARNLrGFPFTnhlKLEERRR 166 Desulfotalea ps...
ABH10974     104 Hkg---ykMDGSMKHv--TDMDvkk-----itEDLSTstk----------skIISTRIRVARNLsFFPLNpggSRTTREK 163 Suberites fuscus
XP_001450149 105 Hk------IEGETKQe--HDWNipvd--eyvlTKIDPkl------------eKVSMRARVARNVkGWNLPplmSKEERIN 162 Paramecium tetr...
ABH10968      80 Hk-----vDLLKTKHvneWSLDsepd--lpenAQLDLskfg--------lppDISIRMRTGRNLnQFPLPgsmTKQDRIN 144 Aphrocallistes ...
Feature 1                                                        #                               
3B6R_B       154 IEKLAVEALSSLDG------DLAGRYYALKSMt-----eAEQQQLIDDHFLFDKp-vSPLLLASGMARd-----WPDARG 216 human
1M15_A       148 MEEKVSSTLSSMED------ELKGTYYPLTGMs-----kATQQQLIDDHFLFKEg--DRFLQTANACRy-----WPTGRG 209 Limulus polyphemus
NP_726252    220 AERLMMSKIFTTHFadaigeTDSGNYYTMTELlep--dsEVTMMLSSLGLMIPLl-dTKDLVQAAESTafngalWPYGRG 296 fruit fly
XP_002293099 148 VQRLIEECTESFESp----kLSNGVFLPVLSMt-----nDQNLDLIERHLLFDNp--NEWTIAAGLGRd-----WPDGRA 211 Thalassiosira p...
XP_001641418 153 IEQAIQSALKRLKG------EFKGKYYSIVDLp-----eSDQKHLTANNLMLVHn--TPEMTCSERSRd-----WPDARG 214 starlet sea ane...
ABN49967     169 LEKKMCEAFDKLKAm----pEYGGGYNSLTPDhpdhiseEAYRQLVKDHIMFKDmaaDPYLASAGIAAd-----WPYGRG 239 Monosiga brevic...
CAG35764     167 LEEKIVAALSVLAD------DLRGEYHSFELLg-----aEKMAALRAEKLIFSKg--DRFQEAAGFNAd-----FPKSRG 228 Desulfotalea ps...
ABH10974     164 IAEHMDKVFADLPD------DLKGDFFRHTTMs-----dQQRQQLIDDHFLFRGk--DKMQAASGYHQe-----WPHGRG 225 Suberites fuscus
XP_001450149 163 FESKMVQAFENFGIk----gKYHSLTPGHKNQit----qEQADELIKRHFLFNDmttDNHLTSSGVASd-----WPFGRG 229 Paramecium tetr...
ABH10968     145 LELEMGGTFKKLISn----pKYGGQYYSLTPGhpsfienDQYLKLVEDHLMFKDmssDTYLVSAGISQd-----WPFGRG 215 Aphrocallistes ...
Feature 1                         #       #                                                      
3B6R_B       217 IWHNDn------kTFLVWVNEEDHLRVISMQKG---GNMKEVFTRFCTGLTQIETLFksk---dyeFMWNPHLGYILTCP 284 human
1M15_A       210 IFHNDa------kTFLVWVNEEDHLRIISMQKG---GDLKTVYKRLVTAVDNIESKLp--------FSHDDRFGFLTFCP 272 Limulus polyphemus
NP_726252    297 AFVNSa------nNMAVWLNCQEHLRIISTTSSkepADMGAAYTRVGRAITYLETQLh--------FKESYLLGYLQSRP 362 fruit fly
XP_002293099 212 LFANVpdvqtetpDFMIWVNEEDHLRICCLRDG---GDIQGVFTTLMTGVRELESELrkr---gwqFAYDKRLGFLTTCP 285 Thalassiosira p...
XP_001641418 215 IFFTSd------kTFVVHVNEADHLKVICWSQG---SDLFDTYDRFQRGLSQLEEELkqn---deeFALSDHLGYIVSDP 282 starlet sea ane...
ABN49967     240 CYVSEd------rGFIIWVGEEDHLRIMCMRKG---TVLNEVFDRLKTALDVVNGIEgp------sFAMSPDYGVVTSCP 304 Monosiga brevic...
CAG35764     229 IFFSAd------kGLRIWLGEEDHMRIISQEGS---ADLAAVFNRLGRALTTLEASLd--------FVRDESYGYLSSCP 291 Desulfotalea ps...
ABH10974     226 IFVSKd------kTFIVWVNEGDHLHIISMEQG---GDVRSVFSRLSRGIEAIEKGLkrvtgraevFMTDPILGVITCCP 296 Suberites fuscus
XP_001450149 230 IWISEd------eTKMIWVGEEDQLRIISIVQG---NDLGKVDQSLHQLLTGIEKSGl-------eFAEHPVYGVITTCP 293 Paramecium tetr...
ABH10968     216 CYVSEd------rSTIIWVGEEDHLRIISMKKG---TLLNNAYNNLKEALDITEPLIng------gFAHSKEFGVVTSCP 280 Aphrocallistes ...
Feature 1               # ###                           # #             #                        
3B6R_B       285 SNLGTGLRAGVHIKLPNLGkh----eKFSEVLKRLRLQKRGTGGVDtaa-vgGVFDVSNADRLGfsEVELVQMVVDGVKL 359 human
1M15_A       273 TNLGTTMRASVHIQLPKLAkd---rkVLEDIASKFNLQVRGTRGEHtes-egGVYDISNKRRLGltEYQAVREMQDGILE 348 Limulus polyphemus
NP_726252    363 SYLGTGLKMTTIVKLTNLMke---mdNLRHLCSVRGLSMVTNRLSK------LTVRLVNMQSMGvvEYVLFQDYCTAVTN 433 fruit fly
XP_002293099 286 TNVGTTMRASVHVRLVNLGrl----pGFAELVERLKLEVRGKYGETdkq-ttGVFDISNAERLGksEVHLINVMVEGVAK 360 Thalassiosira p...
XP_001641418 283 RHLGTAMEVRMRVQFRHLAtd----nRLHFALKCLNMKRCRQGKILavqllqGMVEGINKLSGLeaLLDQGHEIHEALND 358 starlet sea ane...
ABN49967     305 TNLGTGMRASVHIGLPNLTadg-tdaKAKEVCRPLGLSVRGIGGEHtpigegGVCDISPSARFCitEAQIITALYTGIKL 383 Monosiga brevic...
CAG35764     292 TNIGTTMRAGVHIYLEKLNcn---rqLLDALTEKHDLQIRGTGGEKtev-dgAVFDISNRRRLGisERQIITGLHAGLQE 367 Desulfotalea ps...
ABH10974     297 SNLGTAMRGSVHIRVPKLIasw-gfeKLDTLARSKDCQARGSSGEHse--vkDRIDVSNWRRLGfsESSLVQDMIRFANS 373 Suberites fuscus
XP_001450149 294 TNMGTGKRQSILGKFPNITnggkdekKLKDIARQFGLQARGMGGEHsamdheGTADISPSARFGvtEAVVTKRLYEGLIK 373 Paramecium tetr...
ABH10968     281 TNIGTALRASIHLKIPKLMen---ekDAKAFIKSLGMSVRGKGGEHtamgadGLVDISPSSRFCitEARIVATLYKGIKT 357 Aphrocallistes ...
Feature 1               
3B6R_B       360 LIEMEQR 366 human
1M15_A       349 MIKMEKA 355 Limulus polyphemus
NP_726252    434 ILSLEKD 440 fruit fly
XP_002293099 361 LIELERR 367 Thalassiosira pseudonana CCMP1335
XP_001641418 359 LQDAPVV 365 starlet sea anemone
ABN49967     384 LKEEEDK 390 Monosiga brevicollis
CAG35764     368 IIEAEKS 374 Desulfotalea psychrophila LSv54
ABH10974     374 ISDEEDK 380 Suberites fuscus
XP_001450149 374 LYEIEAQ 380 Paramecium tetraurelia strain d4-2
ABH10968     358 ILEKEGE 364 Aphrocallistes beatrix

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