2ITB


Conserved Protein Domain Family
MiaE

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cd07910: MiaE 
Click on image for an interactive view with Cn3D
MiaE tRNA-modifying nonheme diiron monooxygenase, ferritin-like diiron-binding domain
MiaE is a nonheme diiron monooxygenase that catalyzes the posttranscriptional allylic hydroxylation of a modified nucleoside in tRNA called 2-methylthio-N-6-isopentenyl adenosine (ms2i6A). ms2i6A is found at position 37, next to the anticodon at the 3' position in almost all eukaryotic and bacterial tRNA's that read codons beginning with uridine. The miaE gene is absent in Escherichia coli, a finding consistent with the absence of the hydroxylated derivative of ms2i6A in this species.
Statistics
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PSSM-Id: 153119
View PSSM: cd07910
Aligned: 35 rows
Threshold Bit Score: 202.405
Threshold Setting Gi: 162457652
Created: 3-Oct-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 10 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                    #                              #  #                
2ITB_A       10 AFLGCPTPDAWIEAAladqETLLIDHKNCEFKAASTALSLIAKYnthldLINXXSRLAREELVHHEQVLRLXKRRGvpLR 89  Pseudomonas putida
NP_898028    14 RWLKAPTSWSWVEQAnacpVEVLIDHAHCERKAAGSAVQMMFRYlcepgLGEVLSPLAREELEHFEQVLVVIKARGryLE 93  Synechococcus sp...
Q8YT06       10 NVLKQTTSEAWIEQAianlDIILLDHSHCERKAAGVALNFMFRYpsntkMVRELTAIAREELEHFELVNQWLERRNipLA 89  Nostoc sp. PCC 7120
ZP_01305721  14 GYLHCRTPKEWIDAAlqypETILIDHAANELKAAQSAMTLMSKNphkldILDKMSKLAREELLHFEQVMKILKKRGikYQ 93  Oceanobacter sp....
YP_475588    12 LQLASPTSAAWIDQAirhlDLILLDHANCEKKAAGNALSLLFRYpangrLVEALSPLAREELQHFERVHRHLQRLGipIR 91  Synechococcus sp...
ZP_05112001  14 EFLQVKTPQLWLDHAaqqiPLLLIDHAHCERKAAATAINFMSKYpekkeLIDVMSPLAREELLHFEKVVNVMTKRGieYG 93  Legionella dranc...
YP_126822    14 DFLKVKTPETWLHYAaaniPLLLLDHAHCERKAAGTAINFISKYpekaeLIAIMAPLAREELLHFEKVIDIMKQKGifYA 93  Legionella pneum...
NP_865939     2 LHLHSESTKRWLVQVdnhlDELLLDHAHCERKAASTAMNLMNAYtenldICREMATIVEEELEHFFMVIDILKKRDipFR 81  Rhodopirellula b...
ZP_01090136   6 LHLQSTSSERWLTQVtenlDLLLIDHAHCEKKAAGVAMNMIFAYvenedLCRAMTEIVNEELEHFHLVLELLHERGvrFC 85  Blastopirellula ...
ZP_03631354   2 LLFREKVSADWLPMVkanlPAILVDHAHLERKAATSALNLEKYRdl-ydRVDELNAIAIEELQHFQLVLNLLKKRGipFS 80  bacterium Ellin514
Feature 1                  #              #  #    #                               #  #   #      
2ITB_A       90 PVSAGRYASGLRRLVRah-ePVKLVDTLVVGAFIEARSCERFAALVPh---lDEELGRFYHGLLKSEARHYQGYLKLAHN 165 Pseudomonas putida
NP_898028    94 PLPSPGYGAELAKHIRka-ePHRMLDSFLVAGLIEARSHERMALLAEhs--pDPELRQLYGDLLTSEARHFGLYWTLAES 170 Synechococcus sp...
Q8YT06       90 PLPPPPYGAGLKAAARtn-ePERFLDSLLVTGLIEARSHERLGLLAThc--pEPELAKFYKGLMASEARHFGTYWVLADT 166 Nostoc sp. PCC 7120
ZP_01305721  94 ALKASPYAGKMAKFIRnt-nDGILVDSLIIGSIIEARSCERFHALAPy---lDEELEKFYISLLKSESRHFKDYLGLAQK 169 Oceanobacter sp....
YP_475588    92 PLSAAPYASRLNQHLRrs-ePEHLLDALLVAAIIEARSHERLGLLGIha--pDPELRQFYLWLAAAEERHWQLYLDLALE 168 Synechococcus sp...
ZP_05112001  94 PLQPSGYAMQMHKRVTrrdgVERLCDQLIIGAIIEARSCERFHAIIPyi--eDEELAKFYSTLIKSEARHFEDYLRLAKL 171 Legionella dranc...
YP_126822    94 PLQPSDYASNLHKHVTnkdgIERLCDQLIIGAIIEARSCERFNSIIPyi--eDQDLAKFYKTLVKSECRHFEEYLHLAKL 171 Legionella pneum...
NP_865939    82 RIAPGPYGRKLTALIRqn-dPNRAVDRLLVASLIEARSCERFRLLADhvadrEPELSAFYAGLFESEARHHTTYVKLAEH 160 Rhodopirellula b...
ZP_01090136  86 RIKPSKYGEQLHALVRks-ePERAVDRLLVAGLIEARSCERFGILRErl--eDQRLANFYDQLFESEARHHSAYVRLAKQ 162 Blastopirellula ...
ZP_03631354  81 QPYPSPWITGLMRAVRng-nRYQAIDHLVSFALIEGRSCEKFQFLAEglrsiDVELADFYGGLVESEGNHYATYLLMAKA 159 bacterium Ellin514
Feature 1                               
2ITB_A      166 ygd-eaDIARCVELVrAAEXELIQ 188 Pseudomonas putida
NP_898028   171 rwsrevIKPRLEELA-EHESRALD 193 Synechococcus sp. WH 8102
Q8YT06      167 yfdreiVSQRLDELA-IVESELLE 189 Nostoc sp. PCC 7120
ZP_01305721 170 ysk-epIEERVQFFL-QKEQQAIL 191 Oceanobacter sp. RED65
YP_475588   169 qfpadeVQERLDTLL-QVEAEILS 191 Synechococcus sp. JA-3-3Ab
ZP_05112001 172 yg--gdIKQRTTMFL-ALENEFIL 192 Legionella drancourtii LLAP12
YP_126822   172 yg--gsVDERISEFL-TIENSFIL 192 Legionella pneumophila str. Lens
NP_865939   161 ftsremVRERLTELS-KLESEIIA 183 Rhodopirellula baltica SH 1
ZP_01090136 163 yaddatVMRRLEELA-AMEAEIIA 185 Blastopirellula marina DSM 3645
ZP_03631354 160 ide-eeTDRRLDFYL-DLDAKLIR 181 bacterium Ellin514

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