3G3O


Conserved Protein Domain Family
PolyPPase_VTC2-3_like

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cd07892: PolyPPase_VTC2-3_like 
Click on image for an interactive view with Cn3D
Polyphosphate(polyP) polymerase domain of yeast vacuolar transport chaperone (VTC) proteins VTC-2, and -3 , and similar proteins.
Saccharomyces cerevisiae VTC-1, -2, -3, and -4 comprise the membrane-integral VTC complex. VTC-2, -3, and -4 contain polyP polymerase domains. S. cerevisiae VTC-2,and -3 belong to this subgroup. For VTC4 it has been shown that this domain generates polyP from ATP by a phosphotransfer reaction releasing ADP. This activity is metal ion-dependent. The ATP gamma phosphate may be cleaved and then transferred to an acceptor phosphate to form polyP. PolyP is ubiquitous. In prokaryotes, it is a store of phosphate and energy. In eukaryotes, polyPs have roles in bone calcification, and osmoregulation, and in phosphate transport in the symbiosis of mycorrhizal fungi and plants. This subgroup belongs to the CYTH/triphosphate tunnel metalloenzyme (TTM)-like superfamily, whose enzymes have a unique active site located within an eight-stranded beta barrel.
Statistics
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PSSM-Id: 143630
View PSSM: cd07892
Aligned: 11 rows
Threshold Bit Score: 471.079
Threshold Setting Gi: 223645268
Created: 7-Aug-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 15 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:based on similarity to Saccharomyces cerevisiae VTC4
  • Comment:This active site includes the substrate binding and acceptor pockets, and putative metal binding residues.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                  #                                                                     
3G3O_A        36 NDIDXNFRSFKFWVHNDNLXEVKTRILRHLPVLVYANVPSenddlvnrfesdisnndei----------------vgsss 99  baker's yeast
Q96U59       206 THNGEKYTAYKFWVHPDNLLEVKTYILRRLPALVYSHQSSkew------------------------------------- 248 Neurospora crassa
O13718       195 NSSMYRRRTFRFWVHPDNVMEVKTYIMRHLPVLYYSGKQGfdkdqn---------------------------------- 240 fission yeast
Q6CCD8       187 NDSDLNYTSYKFWVHSDNLMEVKTRILRHLPILVYNNQNDsq-------------------------------------- 228 Yarrowia lipoly...
EDK36197     182 EDTTQDVKSFKFWIHRDNLMEVKATILRHLPVLVYNNDNYeddddd---------------------------------- 227 Pichia guillier...
EDP56059     210 RSGQDAYSSYKFWVHEDNILEVKTYILRRLPVLIYNPKSSrdfe------------------------------------ 253 Aspergillus fum...
CAX39923     207 GATNGEFQSFKFWIHPDNLMEVKTTILRHLPVLIYNSTNNddddddddde------------------------------ 256 Candida dublini...
XP_001934375 218 NAVGEDFTSYKFWVHMDNLFEVKTVILRRLPVLVYNPQTSkvae------------------------------------ 261 Pyrenophora tri...
CAY69314     215 DSGSDNFKVLKFWVHPENLMEIKTTILRHLPALIFTDESGddd------------------------------------- 257 Pichia pastoris...
NP_116651    197 NDIDMNFRSFKFWVHNDNLMEVKTRILRHLPVLVYANVPSenddlvnrfesdisnndei----------------vgsss 260 baker's yeast
NP_015306    199 NLEDASFKSYKFWVHDDNIMEVKARILRHLPALVYASVPNenddfvdnlesdvrvqpearlnigsksnslssdgnsnqdv 278 baker's yeast
Feature 1                             #                       # #                #              #
3G3O_A       100 stssvehglgarsFDPLINTLYFDNEHFELYNDKLLKl-NSAPTLRLRWTGQLs-DKPDIFLEKKTLIedeatgkseFDL 177 baker's yeast
Q96U59       249 ----------dgdEDPTITSLYFDNANFQLYYQKVERe-AEASSLRLRWYGQLs-QNPEIVFEQKIIHdn-----gtSEE 311 Neurospora crassa
O13718       241 --------gvsgiLDPISTCLYLDNSNFDLYSQNLERs-EQAYSLRLHWYGKLt-PKTDIIVERMVRQgst----lsHSE 306 fission yeast
Q6CCD8       229 -----------dsVDPTLTSLYFDNSDFELYEGKLQKn-DTSPSLRLKWAGKTsdEDSQIYIEKRTVDhs-----iaGGD 291 Yarrowia lipoly...
EDK36197     228 --------ddassNDPVINTLYFDNPNFDLYNDKLIKk-ANAATLRIKWIGKLl-DNPKISVEKKSFDpts---dtfDVN 294 Pichia guillier...
EDP56059     254 ----------slpEDPTITSLYFDNPQFDLYNQKVAKa-PEAGSLRLRWTGDLk-DTPAIFLEKKIVTsd-----dySKE 316 Aspergillus fum...
CAX39923     257 ---deddsnkhitGGQTINCLYLDNDHFDLYNHKLTKl-NNSSTVRIRWVGKLa-DKPKISMERKSFDvns----nfFVD 327 Candida dublini...
XP_001934375 262 ----------gtqRDPTITSIYFDNPNFSLYTDKVNGk-PDAASLRLRWYGHLn-EKPEIVFEKKVIKeg-----daAEE 324 Pyrenophora tri...
CAY69314     258 -----------dlSDPVISTLYFDNRGFELYNNKLLKnlKETPTLRIRWTSKLt-PGKDVTVEKRTFLnet----gvDDD 321 Pichia pastoris...
NP_116651    261 stssvehglgarsFDPLINTLYFDNEHFELYNDKLLKl-NSAPTLRLRWTGQLs-DKPDIFLEKKTLIedeatgkseFDL 338 baker's yeast
NP_015306    279 eigksksvifpqsYDPTITTLYFDNDFFDLYNNRLLKi-SGAPTLRLRWIGKLl-DKPDIFLEKRTFTentetgnssFEE 356 baker's yeast
Feature 1                                                                                        
3G3O_A       178 TKLQLKQKFINGFIFEgd----------------------kKFKEQTLKklkesgtagrdlERLEEDFSEIQNFIIKNEL 235 baker's yeast
Q96U59       312 RKFPIKEKYIKPFLDGe-----------------------yKMEKSIQKmerqgq-spadvEEFRSTAEAIQDYIRSNKL 367 Neurospora crassa
O13718       307 DRFTIREKKVRELLSGr------------------------YDFRKVEDdhst-----tasDQKKKLIEDVEQLIVDNHL 357 fission yeast
Q6CCD8       292 ERLPIKEKYIAGFINGt-----------------------yHMEKTVQKmksshv-pdyqvEGYEKSVENIQDFIRENSL 347 Yarrowia lipoly...
EDK36197     295 EKVVLKEKYLDTFVKGn-----------------------fSPEKLIKKmekr----gensKDFADTVQNLESFITEHHL 347 Pichia guillier...
EDP56059     317 VKVQLKKKHVQEFLEGk-----------------------yTFEKQLHRmeglnngesaeaETLKKDVEELQSFIKENNL 373 Aspergillus fum...
CAX39923     328 DKIELRQKYINQYVIQk------------------------EIPKKLVK------------LNDANSVKKLLDFIKEYNL 371 Candida dublini...
XP_001934375 325 VRFPIKAKYVQSFLEDh-----------------------yHMEKSIEKmeyrpnkdqkklDNFKKAVTDIQGFIKEQKL 381 Pyrenophora tri...
CAY69314     322 IKLKLKEKYINDFIKLqsdeddeesldhstdyasskefnvnKFTSKLTKrnlp----qltiQDERQKFISLQKFIREQRL 397 Pichia pastoris...
NP_116651    339 TKLQLKQKFINGFIFEgd----------------------kKFKEQTLKklkesgtagrdlERLEEDFSEIQNFIIKNEL 396 baker's yeast
NP_015306    357 IRLQMKAKFINNFIFKndpsyk-------------nylinqLRERGTQKee---------lEKLSRDFDNIQNFIVEEKL 414 baker's yeast
Feature 1               # #                #                                                     
3G3O_A       236 QPVFRTVYTRTAFQI-PGDDKIRVTIDSNIVFIKEDSFDRERPIRDPNTWHRTDIDanv-anPLKFLRGGEYAKFPYSVX 313 baker's yeast
Q96U59       368 EPVVRANYVRTAFQN-PGDDRIRLSIDTDIAFIREDTLDRDRPCRDPNNWHRTDIDdsnmtyPFKNINQSEVSLFPYAVL 446 Neurospora crassa
O13718       358 QPVLRSVYTRTAFQI-PGDDEVRINLDSDWVMIREDSLDIERPCRDPEDWHRHDIDda--dfPYKHLRKGEYSRFPYSVL 434 fission yeast
Q6CCD8       348 QPVCRAQYTRTAFQI-PGDNRVRVIMDNDILFIKEDAFDKDRPIRDPNNWHRTDIDav--sdPKKVLRKGEYSKFPYAVL 424 Yarrowia lipoly...
EDK36197     348 QPAVRSVYRRTAFQI-PGDDKIRVVIDSDLMFIREDSFDEQRPIRDPKKWHRTDIDski-snPYSLLRSGEYSKFPYSVM 425 Pichia guillier...
EDP56059     374 QPMLRANYTRTAFQI-PGDDRIRISLDTNLALIREDALDEERPCRDPTDWHRRDIDdsgmqyPFDGIRTGEVNRFPHALL 452 Aspergillus fum...
CAX39923     372 QPILRTTYNRTAFQI-PGDDRIRIIIDSNLTFIREDSFDPQLPIRDPQQWHRLDLDns--ksPQQFLRKGEFVKFPFSTM 448 Candida dublini...
XP_001934375 382 QPMVRANYTRTAFQI-PGDDRVRISLDTNLALIREDALDLDRPCRDPDDWHRRDIDnaqmefPFKAIKKGEIHKFPYALL 460 Pyrenophora tri...
CAY69314     398 EPVLRTVFTRTVFQVpGDEDKIRVVIDSDMLYIREDSFDKDRPIRDPKSWHRRDLDqpnlvnPFDVLREGEYAKFPYSIM 477 Pichia pastoris...
NP_116651    397 QPVFRTVYTRTAFQI-PGDDKIRVTIDSNIVFIKEDSFDRERPIRDPNTWHRTDIDanv-anPLKFLRGGEYAKFPYSVM 474 baker's yeast
NP_015306    415 QPVLRATYNRTAFQI-PGDQSIRVTIDSNIMYIREDSLDKNRPIRNPENWHRDDIDsni-pnPLRFLRAGEYSKFPYSVM 492 baker's yeast
Feature 1        # #                                             ####                         
3G3O_A       314 EIKVKssldssxsas--sxisnvklpKKHGQWLNDLTNSHL-VKEIPKFSIFVQGVASLYgdde-kldiLPFWLPDL 386 baker's yeast
Q96U59       447 EIKLKed-----------------qnRKRPVWVTDLMASHL-VHPCPRFSKFMQGVASLFedy---vnrLPFWLPDL 502 Neurospora crassa
O13718       435 EIRECvr-----------------ydEDEPLWISELRNSHL-ISEIDGFSKYEHGVAILFeky---vslLPMWVFSM 490 fission yeast
Q6CCD8       425 EFRVQnrpgvdtadtgnsiitsssvyRKHGKWIDELTSSHL-VKEVPKFSKFIQGIASLYgedd-klnfLPLWLSVL 499 Yarrowia lipolytica
EDK36197     426 EIKIKts-----------------knNKRLLWVDELVHSHL-VKEVPNFSKFVHGVSVLFledd-klqnIPMWYHDL 483 Pichia guilliermon...
EDP56059     453 EIKLRg-------------------sAHHIEWVNELMVSHL-VKEAPRFSKFVHGVAQLFedh---vnsFPFWLSEL 506 Aspergillus fumiga...
CAX39923     449 EIKIKkss---------------aknFKKLQWVNELINSSYlVKEIPNFSKFIHGVASLFledd-kldnIPMWFNEL 509 Candida dubliniens...
XP_001934375 461 EIKVKg--------------------MKKYEWIEDLMHSHL-VKESPRFSKFVHGVAKLFedn---vntFPFWHQRG 513 Pyrenophora tritic...
CAY69314     478 EIRVNeriy-------------snpnSKTYKWVRDLVNSHL-VKEVPNFSKFIQGISSLFledd-nldiLPFWLPDL 539 Pichia pastoris GS115
NP_116651    475 EIKVKssldssmsas--smisnvklpKKHGQWLNDLTNSHL-VKEIPKFSIFVQGVASLYgdde-kldiLPFWLPDL 547 baker's yeast
NP_015306    493 EIKVInqd---------------nsqMPNYEWIKDLTNSHL-VNEVPKFSLYLQGVASLFgeddkyvniLPFWLPDL 553 baker's yeast

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