2FBL


Conserved Protein Domain Family
CYTH-like_CthTTM-like

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cd07761: CYTH-like_CthTTM-like 
Click on image for an interactive view with Cn3D
Clostridium thermocellum (Cth)TTM and similar proteins, a subgroup of the CYTH-like superfamily
CthTTM is a metal dependent tripolyphosphatase, nucleoside triphosphatase, and nucleoside tetraphosphatase. It hydrolyzes the beta-gamma phosphoanhydride linkage of triphosphate-containing substrates including tripolyphosphate, nucleoside triphosphates and nucleoside tetraphosphates. These substrates are hydrolyzed, releasing Pi. Mg++ or Mn++ are required for the enzyme's activity. CthTTM appears to have no adenylate cyclase activity. This subgroup consists chiefly of bacterial sequences. Members of the CYTH-like (also known as triphosphate tunnel metalloenzyme (TTM)-like) superfamily have a unique active site located within an eight-stranded beta barrel.
Statistics
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PSSM-Id: 143626
View PSSM: cd07761
Aligned: 25 rows
Threshold Bit Score: 163.556
Threshold Setting Gi: 227991152
Created: 28-May-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:putative active site [active site]
Evidence:
  • Comment:Based on mutational analysis of Clostridium thermocellum TTM. These residues are important for tripolyphosphatase and ATPase activity.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1         # #                                            # #                #         # #
2FBL_A         4 TEIERKFLV------ATFPDg------eLHAVPLRQGYLTtptdsIELRLRQQg------tEYFMTLKSEGg-lSRQEYE 64  Nitrosomonas eu...
ZP_04038218    3 REIERKFLVr----sPEWKTg-------AAGVLYRQGYLCrq-eaRTVRVRIAg------gQAYLTIKGQAqgvTRLEYE 64  Meiothermus rub...
ACR11977       3 EEIERKFLL------NELPAdia---twPQPAAILQGYLQkd-qhKDLRIRKKg------nKYTLTAKHGTg-lVREENE 65  Teredinibacter ...
ZP_03472821    1 MEIERQFLV------DIIPAl------pDTFDSILQGYVSl---fPEIRIRSVrple-gpeKFYLTVKRGEg-lVRDEWE 63  Mitsuokella mul...
ZP_02092578    1 MEIERKWMV------TGWPEgl----plEEEFTMRQGYISv---qPTVRIREEaltg-gstDYILCFKSAGg-lAREEIE 65  Faecalibacteriu...
ZP_03608948   31 IEIERKFILrdaaviSELKArd----igTEQKEVTQIYVKit-plEEIRFREAs------gVFTITQKSGIg-lAREENE 98  Campylobacter r...
ZP_02080611    1 MEIERKFLL------SGFPDlp-----eLERAEVFQGYLGi---sPEVRIRSKrvg--eaeTYRLCFKGEGs-lVREEIE 63  Clostridium lep...
ZP_04454444    1 MEIERKWIV------TDWPRgl----slLYEQTMRQGYLSv---rPTVRIREEkmsd-getHDILCFKSGSg-iVRREIE 65  Shuttleworthia ...
ZP_02948570    1 MEIERKWLF------KSIAApkg--tkiLGEYFYKQAYISi---nPEVRIRSRrkaeddnfTYALCIKSKGt-lERIEVQ 68  Clostridium but...
YP_001408871   1 MEIERKFLLk----nSQILEflsaesvsFKRLEILQFYTKit-dnEEVRYRSEd------aKFIKTIKIGKd-lVREENE 68  Campylobacter c...
Feature 1                                # #              #            # #                       
2FBL_A        65 IQIDVTQFEMLWp---ATEGRRVEKTRYSGKlp-dGQLFELDVFAGHLSPLMLVEVEFLsedaAQAFIPP-----PWFGE 135 Nitrosomonas eu...
ZP_04038218   65 YPIPLAEAQELLe--hLCLKPLIEKTRYCIEf--rGRVWEVDEFAGENQGLVVAEVELEs--pDQPLELP-----DWVGE 133 Meiothermus rub...
ACR11977      66 QEIDKTLFDILWp---FTAEARIEKLRYTRVi--dEGEMVIDKYLGDLSGLLLMEVEFNdeqlARTYSPP-----LNFAK 135 Teredinibacter ...
ZP_03472821   64 TEISSREFSHLVec-lEKGTWFIEKRRYRLPla-gDHVAEYHRHSGHLKGFDYVEVEFRseeeARAFEPP-----YWFGR 136 Mitsuokella mul...
ZP_02092578   66 RSIDKDLFVQLEe--kIIGKPLIKKVRRSYRlpdgAVLEVNHVDEGLPTAFWYAEVEYPtveaALSWQPAacglvAYLND 143 Faecalibacteriu...
ZP_03608948   99 SETDAKSFKKALk---NVVAAPIKKTRFLFRl--dGAACNVDIFHGALEGLVTLEAEFAsereAAEFNPPef-iaAHIAS 172 Campylobacter r...
ZP_02080611   64 LDLDKATFESLKk---LLPKPMIRKEYRIYRlengLRLECSRVDPGTDTEFYYAEVEFQdqqqAEIFSPP-----DFLGK 135 Clostridium lep...
ZP_04454444   66 IEISPDQFQELEe---MIALPLIPKIRRTYAlsdgLKLEVNHVDCGSDTEFWYAEIEYPteeaARSWQPSpl-laSYLGK 141 Shuttleworthia ...
ZP_02948570   69 KELTEKEFNELMvvgnIKEEDFIEKHFYKYSvn-nHELTLGVTDIGRSTEFFYGEIEFNssedAISFTPP-----KWFGQ 142 Clostridium but...
YP_001408871  69 CECDKKEFKNAFk---SHIGAPIYKDRYLFKl--nNNPCNIDVFKGAQEGLCTLEVEFKdeaeAVYFNLPkf-lrNFIEA 142 Campylobacter c...
Feature 1                       
2FBL_A       136 EVTEDKRYKNKALAL 150 Nitrosomonas europaea
ZP_04038218  134 EVTHDPRYLNASLVE 148 Meiothermus ruber DSM 1279
ACR11977     136 EVTDDPRYRNAALAA 150 Teredinibacter turnerae T7902
ZP_03472821  137 EVTEDPRFSYGKLAR 151 Mitsuokella multacida DSM 20544
ZP_02092578  144 EVTNQPGQSMGAYWV 158 Faecalibacterium prausnitzii M21/2
ZP_03608948  173 EVTEDERYKNKNLAL 187 Campylobacter rectus RM3267
ZP_02080611  136 EVTYDGSYRMKAYYQ 150 Clostridium leptum DSM 753
ZP_04454444  142 DVSEEPGQSMGAYWL 156 Shuttleworthia satelles DSM 14600
ZP_02948570  143 EVTDDPQYKMSNFWQ 157 Clostridium butyricum 5521
YP_001408871 143 DVTCDQRYKNKYIAL 157 Campylobacter curvus 525.92

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