3BHD,2JMU,3BHD


Conserved Protein Domain Family
ThTPase

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cd07758: ThTPase 
Click on image for an interactive view with Cn3D
Thiamine Triphosphatase
ThTPase is a soluble cytosolic enzyme which converts thiamine triphosphate (ThTP) to thiamine diphosphate. This catalytic activity depends on a divalent metal cofactor, for example Mg++. ThTPase regulates the intracellular concentration of ThTP, maintaining it at a low concentration in vivo. ThTP acts as a messenger in cell signaling in response to cellular stress, and in addition, can phosphorylate proteins in certain tissues. There is another class of membrane-associated enzymes in animal tissues which also convert ThTP to thiamine diphosphate, however they do not belong to this subgroup. This subgroup belongs to the CYTH/triphosphate tunnel metalloenzyme (TTM)-like superfamily, whose enzymes have a unique active site located within an eight-stranded beta barrel.
Statistics
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PSSM-Id: 143625
View PSSM: cd07758
Aligned: 19 rows
Threshold Bit Score: 168.324
Threshold Setting Gi: 167537795
Created: 5-Jun-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 29 residues -Click on image for an interactive view with Cn3D
Feature 1:putative active site pocket [active site]
Evidence:
  • Comment:The active site pocket based on NMR titration experiments of mouse ThTPase. It includes the Mg2+ and ThTP binding sites, and key hydrophobic residues.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1         #####                                   #             # # #        # #         
3BHD_A        25 IEVERKFLpg----pgTEERLQELGg----TLEYRVTFRDTYYDtpelsLXQADHWLRRREDs---GWELKCPGaagv-- 91  human
2JMU_A         6 IEVERKFApg----pdTEERLQELGa----TLEHRVTFRDTYYDtselsLMLSDHWLRQREGs---GWELKCPGvtgv-- 72  house mouse
3BHD_B        25 IEVERKFLpg----pgTEERLQELGg----TLEYRVTFRDTYYDtpelsLXQADHWLRRREDs---GWELKCPGaagv-- 91  human
NP_001089682  11 IEVERKFVpg----pqVEDKLHSLGa----KLQNELTFRDSYYDtpdcrLTLGDVWLRRRED----SWELKYPPqrgtrg 78  African clawed ...
AAI33924       9 VEVERKFVcd----aeIMRKLQDIGa----ECVGEREFGDQYFDspdflLTLKDFWLRCRDG----QWELKSPAvseap- 75  zebrafish
EEC03674      52 CEVERKFRap----cdIEDRLVRIGa----VLGSRTQFTDRYYDtqdnvLALNDHWLRIRGGas-dHWELKYRKhndtps 122 black-legged tick
EEH60910      63 IEVERKYGa------pADLKAAITTae--gTLTKSIIFGDTYYDtpncdLAAKDVWLRRREK----QWEIKIPVegeggr 130 Micromonas pusi...
XP_001627536   9 LEIEKKFQip----vrFEETLLSNGg----KLTERREFQDVYYDlpdnsLTLKGLWLRKRNT----KWELKHLNarcdp- 75  starlet sea ane...
ACO14570      92 IEVERKFTvp----ecFEALLDKHGfknmsGFEQDEEICDTYYDsqgfdLMIEDYWLRSRNG----DWELKYPVgvhp-- 161 Caligus clemensi
XP_002181211  41 FEVEQKFPlsldslpiLEERLRILGf----EQITTKELTDWYFDnvcsdLVQQDCWLRYREDenqgEWQLKKGVrgqyn- 115 Phaeodactylum t...
Feature 1               # #                                                        # #           
3BHD_A        92 -lgphTEYKELTAEPTIVAQLCKVlradglgagd---------------vaavlgplgLQEVASFVTKRSAWKlvllgad 155 human
2JMU_A        73 -sgphNEYVEVTSEAAIVAQLFELlgsgeqkpag---------------vaavlgslkLQEVASFITTRSSWKlalsgah 136 house mouse
3BHD_B        92 -lgphTEYKELTAEPTIVAQLCKVlradglgagd---------------vaavlgplgLQEVASFVTKRSAWKlvllgad 155 human
NP_001089682  79 lngasTQYLELNCERDIAHRVSEElnipcph---------------------sldlleLQEFASFVTKRRRFElpprn-- 135 African clawed ...
AAI33924      76 aetlcTRYREITSLPVIQSEVSKIiresncsqrkqidqted-tpicstgdtkwlnelgLVCFAEFRTQRCSYVlere--- 151 zebrafish
EEC03674     123 ndsavTIYNEETGEANILTIVKKLlperspvgeav-------------tlkdfvseglLREFACIQTWRSSYKts----- 184 black-legged tick
EEH60910     131 sggerSVFREVEGARSVADELAAAlglpapsdggdggga-----dgeeiltrvmadaaARPFAEFVTERVSWSld----- 200 Micromonas pusi...
XP_001627536  76 tisitDKYHETSDETKIMTELSKHvpmatsl--------------------delvskhCREFASFTTQRTSYQlp----- 130 starlet sea ane...
ACO14570     162 --tgsTLYHETSDVHEISAKLQILvpkknngskrnsm--------ifnnigdficnqiLHKFAELITRRKHYQkd----- 226 Caligus clemensi
XP_002181211 116 gantsTVYEEIEGAKALNAAVDLLakverkadvplpfefagfapfhipklleeisllqLHPFARILTKRASWVstdqcn- 194 Phaeodactylum t...
Feature 1               ## #  #  # # # ##                                                       #
3BHD_A       156 eeepqLRVDLDTADFGYAVGEVEALVhe---eaEVPTALEKIHRLSSXLGVpa---------------------qETAPA 211 human
2JMU_A       137 gqepqLTIDLDSADFGYAVGEVEAMVhe---kaEVPAALEKIITVSSMLGVpa---------------------qEEAPA 192 house mouse
3BHD_B       156 eeepqLRVDLDTADFGYAVGEVEALVhe---eaEVPTALEKIHRLSSXLGVpa---------------------qETAPA 211 human
NP_001089682 136 gskhkVVVDLDTADFGFSVGEVEVLVet---qeEVQSALKEVEEICKKLGVls---------------------dSPVPG 191 African clawed ...
AAI33924     152 --dgaVRVDLDRADFGYCVGEIEVLVpe---ggDVNAALQSITSIAEQLGLns---------------------eKKVKG 205 zebrafish
EEC03674     185 ---grSVVDLDQTDWGYRVAEIEVLLqeadcdaQGKDAMEKATEEISRLAAqldgkplfinpkhaarelgraqfeTRIPC 261 black-legged tick
EEH60910     201 ----gASIDFDHASFGHSVVEVEVMCer---aeQVEDAEKKVEEVAAKLGLep---------------------lTETGG 252 Micromonas pusi...
XP_001627536 131 ---ndVRVDLDVASFGYSVGEIEIVIdd---etGVSEAEQRLEGLAEKLGInw---------------------qKKFPG 183 starlet sea ane...
ACO14570     227 ----nVSIVVDETNWGYKVGEIEMVVkd---klEVPEASKKIDAIARQLNFkkl-------------------klPLIAS 280 Caligus clemensi
XP_002181211 195 mmfwaLTVDLDITDFGYAVGEVEAMIkd---rrDVPRARAAINGLVKRLKPvgt-------------------hsTIAIG 252 Phaeodactylum t...
Feature 1        ####  
3BHD_A       212 KLIVYL 217 human
2JMU_A       193 KLMVYL 198 house mouse
3BHD_B       212 KLIVYL 217 human
NP_001089682 192 KMSTFL 197 African clawed frog
AAI33924     206 KMDVFL 211 zebrafish
EEC03674     262 PRTAFR 267 black-legged tick
EEH60910     253 KLETFI 258 Micromonas pusilla CCMP1545
XP_001627536 184 KVEEFL 189 starlet sea anemone
ACO14570     281 KISTRE 286 Caligus clemensi
XP_002181211 253 KLEYYL 258 Phaeodactylum tricornutum CCAP 1055/1

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