Conserved Protein Domain Family
CYTH-like_Pase_CHAD

?
cd07756: CYTH-like_Pase_CHAD 
Uncharacterized subgroup of the CYTH-like superfamily having an associated CHAD domain
This subgroup belongs to the CYTH-like (also known as triphosphate tunnel metalloenzyme (TTM)-like) superfamily. Members of this superfamily hydrolyze triphosphate-containing substrates, require metal cations as cofactors, and have a unique active site located at the center of an eight-stranded antiparallel beta barrel tunnel (the triphosphate tunnel). A number of proteins in this subgroup also contain a C-terminal CHAD (Conserved Histidine Alpha-helical Domain) domain which may participate in metal chelation or act as a phosphor-acceptor. The name CYTH originated from the gene designation for bacterial class IV adenylyl cyclases (CyaB) and from thiamine triphosphatase. Class IV adenylate cyclases catalyze the conversion of ATP to 3',5'-cyclic AMP (cAMP) and PPi. Thiamine triphosphatase is a soluble cytosolic enzyme which converts thiamine triphosphate to thiamine diphosphate. This domain superfamily also contains RNA triphosphatases, membrane-associated polyphosphate polymerases, tripolyphosphatases, nucleoside triphosphatases, nucleoside tetraphosphatases and other proteins with unknown functions. Proteins of this subgroup have not been characterized.
Statistics
?
PSSM-Id: 143624
View PSSM: cd07756
Aligned: 68 rows
Threshold Bit Score: 155.469
Threshold Setting Gi: 120612667
Created: 28-May-2009
Updated: 2-Oct-2020
Structure
?
Aligned Rows:
 
Feature 1:putative active site [active site]
Evidence:
  • Comment:Based on similar proteins with structure, and on NMR titration experiments of mouse Thiamine triphosphatase, and on mutation analysis of Clostridium thermocellum TTM.
  • Comment:This active site includes the metal binding and triphosphate binding sites.

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1        # # #                                          #               # #          #   
Q8Z3N0         4 EIELKFIvnhd---avdaLRNHLHTLGGehh--------apSQLLNIYFETPDNWLRRHDMGLRIRGENGRYEMTMKIAG 72  Salmonella typhi
ZP_02002706    4 ETELKLYiape----daeKFMQHPLLQQansr------qapLKLYNTYFDTPEHTLLQHGVGLRVRQIGKKRIQTLKTAD 73  Beggiatoa sp. PS
YP_001353616   2 EVELKLLirpq----diqSFKKHVLIRKyaqg-----tpveQNQLDIYFDTDDHDLKQCGAGLRVRQVGKAWTQTLKAGG 72  Janthinobacteri...
YP_546177      4 EIELKLRiake----dipHLRRHAAVRKhvtg-----kavtRRLVSTYYDTPDLQLLDQHVSLRVRRMSGSWFQAVKAAG 74  Methylobacillus...
ZP_01549752    7 EIELKLEl---------eTSAQDALKRAgsidgftasravnKTLQSIYFDTPDQALRKAKISLRVRKSGNSWVQTVKLGT 77  Labrenzia aggre...
YP_411083      4 EIELKLRlspe----dvgRLRGDPLLTSltvs-----rpvsQKLFTAYYDTPDLYLQRHQMALRLRHIGRKWIQSIKGGG 74  Nitrosospira mu...
YP_002670417   6 EIKLKVMq---------eAELDLSALTWltalla--tqpykQHLVSTYFDTADRALMQFGVGLRLRQVDSQWLQTVKCRG 74  Methylophaga th...
ZP_03699028    4 ETELKLRlppnrnaafrrWMAQHAEAAApa----------tEQLLSIYYDTPTLALAQRDIGLRLRRVGDRWLQTVKLGE 73  Chromobacterium...
ZP_02061845    4 EIELKLHiape----qvhLLLEHPLLKPnptq-----pilvERLISRYFDSADLDLWQHGLSMRIREAGGRTIQTLKTAG 74  Rickettsiella g...
YP_002680577   3 EIELKLSvd--------eATLAQLIKAKppegfa-vseqstKKLRSVYYDTPDHTLKKARISLRTRHNGTNWLQTIKQGG 73  Pseudovibrio sp...
Feature 1                  #                                                   # #               
Q8Z3N0        73 rvtggLHQRPEYNVALSEPVLdltql---paevWPDGNLPDGLas--------sVQPLFSTDFYREKWCLdvd--gsrIE 139 Salmonella typhi
ZP_02002706   74 eglggLHQRQEWENEISGDKPnynqf---pkkaLPKWCADKKNlq--------kIKPLFTTDFSRTIWDLildd-gtqIE 141 Beggiatoa sp. PS
YP_001353616  73 svangLHQRHEWESQVHKPEPdlyal---rkliDVHGAWDELIaspr---iaarLHPIFTTMVHRTIWHLqlps-gdeIE 145 Janthinobacteri...
YP_546177     75 hslagLHQRLEWEDIISKGEPdytki--tdpslTPIFDNPALWq---------aLLPIFTTDMQRTEWQLdyq--gshIE 141 Methylobacillus...
ZP_01549752   78 gvmggLSSPVEAEHPVKGRALd-----------FSVIEDPCVHerlleiigdapLSECFETVMKRTVRNLtrdsdgtfIE 146 Labrenzia aggre...
YP_411083     75 eaalgLHQRHEWESMVPTGRPd-----------FSQITDPSLIrlfdgaeshgqLCVLFTTDFQRSKRVLrlvd-gseIE 142 Nitrosospira mu...
YP_002670417  75 eaqdgLHQRKEWEHKLAGPAFde--------glLAETDLAPLIdnka---vwqaIKPVFTTDFERVVMPLqled-gtqVE 142 Methylophaga th...
ZP_03699028   74 qagggLHRRQELETEVSGEALeperiaertirqLLTREDVAGA-----------LAPLFRTDIKRTRWMLrdag-gniLE 141 Chromobacterium...
ZP_02061845   75 kqigdLQHRHEWDQLVTHNKPni--------eqFTDVHLKTKLksil---ghkpLFELFHTDFERRSWNLften-ethVE 142 Rickettsiella g...
YP_002680577  74 smqsgLSKRIELELPIGGIEPd-----------FDAITDEEVRqkllslledkdWGILFETAISRTLTYYtdka-gakIE 141 Pseudovibrio sp...
Feature 1           #                 # #                                    
Q8Z3N0       140 IALDLGDVkage-faePICELELELLRGdtRAVLKLAKQLLSqtgLRQGSLSKAARGYHL 198 Salmonella typhi
ZP_02002706  142 VALDKGEIktet-hscPLSEVELELKSGspDKLYQVALTLQNdlpLIIENKSKAARGYAL 200 Beggiatoa sp. PS
YP_001353616 146 CVLDEGSInkln-ysePICEIELELKSGkaLHLFDFALELLKklpLRIGNENKAERGYAL 204 Janthinobacterium sp. Marseille
YP_546177    142 LSLDDGLLiage-hsqPMIEIELELKGGkvEHLFALALDMQSdiaLTIENVSKAQRGYAY 200 Methylobacillus flagellatus KT
ZP_01549752  147 LAFDSGNIlags-gsqPLTELEMELKSGpsLALFEAARALLKdipFRFSPYSKAERGYRF 205 Labrenzia aggregata IAM 12614
YP_411083    143 FSLDRGVIrtpadftsEICEIELELKSGsaQSLYQFALDILHivpFRLENTSKAERGYRL 202 Nitrosospira multiformis ATCC 25196
YP_002670417 143 MAYDRGVVkagd-kqqNIHEIELELKQGdiHKCQQLAASLKRslaLEYSDISKAEQGYAV 201 Methylophaga thiooxidans DMS010
ZP_03699028  142 VCLDQGTIqgqa-rsmKLNEVEIELKQGhvQAVFDLALQMAStlpLIPDPQSKAERGYAL 200 Chromobacterium sp. 2002
ZP_02061845  143 CVLDQGHVktat-hqtALHEIELELKQGdsQQLYKIAEQLKStipLTVETRSKAMRGYLL 201 Rickettsiella grylli
YP_002680577 142 VAFDSGIAktkt-dehPIHEVELELMEGpvESLYKLAGALMKdrpILFSNSNKAGIGYRL 200 Pseudovibrio sp. JE062

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap