3G3R,3G3Q,3G3U,3G3T


Conserved Protein Domain Family
PolyPPase_VTC4_like

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cd07751: PolyPPase_VTC4_like 
Click on image for an interactive view with Cn3D
Polyphosphate(polyP) polymerase domain of yeast vacuolar transport chaperone (VTC) protein VTC4, and similar proteins.
Saccharomyces cerevisiae VTC-1, -2, -3, and -4 comprise the membrane-integral VTC complex. VTC-2,-3, and -4 contain polyP polymerase domains. S. cerevisiae VTC4 belongs to this subgroup. For VTC4 it has been shown that this domain generates polyP from ATP by a phosphotransfer reaction releasing ADP. This activity is metal ion-dependent. The ATP gamma phosphate may be cleaved and then transferred to an acceptor phosphate to form polyP. PolyP is ubiquitous. In prokaryotes, it is a store of phosphate and energy. In eukaryotes, polyPs have roles in bone calcification, and osmoregulation, and in phosphate transport in the symbiosis of mycorrhizal fungi and plants. This subgroup belongs to the CYTH/triphosphate tunnel metalloenzyme (TTM)-like superfamily, whose enzymes have a unique active site located within an eight-stranded beta barrel.
Statistics
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PSSM-Id: 143623
View PSSM: cd07751
Aligned: 19 rows
Threshold Bit Score: 324.314
Threshold Setting Gi: 226460254
Created: 19-May-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 16 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:based on Saccharomyces cerevisiae VTC4 polyP polymerase
  • Comment:The active site includes the substrate binding and acceptor pockets, and the putative metal binding residues.
  • Structure:3G3R_A; Saccharomyces cerevisiae VTC4 polyP polymerase bound with a substrate analog (adenosine-5prime-[(beta,gamma)-imido] triphosphate) and Mn2+, contacts determined at 4.5A.
    View structure with Cn3D
  • Structure:3G3T_A; Saccharomyces cerevisiae VTC4 polyP polymerase bound with orthophosphate, contacts determined at 4A.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1              #   #                                                                     
3G3R_A         5 KQQNFVRQTTKYWVHPDNITELKLIILKHLPVLVFNTNKEfe---------------------------------rEDSA 51  baker's yeast
EEH57548     231 PIRPFTRKTVKYWVDVRDVLRVKLAVARHLPLLVYDGKKGkkkgkkkgkkngttrgde-ggdgdgdgdgdeifpprDGGK 309 Micromonas pusi...
XP_002261454 218 PPETFERTSTKFLMKYEDIVYTKVKIVKHLPYLIFGLSNQdleehfrnfgqkknkklaaqmdgelddtheknkplnESQQ 297 Plasmodium know...
Q5CS43       271 PPETFLRNTTKYWVHPNNVVSAKVGIIRYVPYLIFGISNSeletlldpymcv------------eghnvvdpktieESQA 338 Cryptosporidium...
Q8SRN4       161 SNCMFIRMTDKYWVHKENVVPVKFYITQHLPIYVFSKGNEekspys------------------------swdknsHDTC 216 Encephalitozoon...
XP_001827737 154 TNLVFVRKTNKYWVHIDNLMALKLSIVKNLPLYVFTNEKGqtpysa-------------------------wnhktHDTC 208 Enterocytozoon ...
EEH60426     288 GAQDFVRTTRKYWVSPEDVSGVKQNIAQHLPVFLVERERVkglveaaagesgdg--------vapgfagltlpshaDSQM 359 Micromonas pusi...
CAL54750     216 PPSTFERRTVKYWVHPRDVVPLQLEILKRLPILDFRDQRVgkddrek-----------------------dqrdvsDRSM 272 Ostreococcus tauri
ACO63196     278 PPTTFERRTRKYWIRPRDVLRVKLATCKHLPVLVFGGRDGraasapsrgak---------------nlalgdvgpvDGCL 342 Micromonas sp. ...
XP_001419043 114 PPSTFERRTVKYWVDPRDAIPVQLAILKHLPILEFSGGEDaeprae-------------------------ddedqDRSL 168 Ostreococcus lu...
Feature 1            #                      # #                                            #     
3G3R_A        52 ITSIYFDNENLDLYYGRLRKdEGAEAHRLRWYGGm------------------------------STDTIFVERKTHRED 101 baker's yeast
EEH57548     310 ITSAYFDSETLDVYKIRLAReQGATLVRARWYGDdadvsgvygdaa-------aardddddddddDDDDVFLERKSHHES 382 Micromonas pusi...
XP_002261454 298 ITSVYLDNEEATCYSNRILRyENAQLIRFRWYNDnd---------------------------gnPDKTIFIERKIHHEE 350 Plasmodium know...
Q5CS43       339 ITSVYLDNMNAECYYNRISRfEDAQLFRLRWYGSns---------------------------gsKEKEIFMERKTHHES 391 Cryptosporidium...
Q8SRN4       217 ISSVYFDNVAFDLYGERLKKlHHSEAIRIRWYTSe------------------------------VPDVVFVERKKHEDG 266 Encephalitozoon...
XP_001827737 209 VTSVYFDNDDFYIYDGRLKKnEGAEAIRIRWYGTis----------------------------kETDTVFIERKKHCEG 260 Enterocytozoon ...
EEH60426     360 TNSVYLDNVHLELYHARLKKaPRAIAIRLRWYGPa------------------------------PTGSVYVERKTHRES 409 Micromonas pusi...
CAL54750     273 ITSVYLDNSNCEVYRSRLVRdQGATLVRCRWYGGg------------------------------RWDDVFIERKTHHES 322 Ostreococcus tauri
ACO63196     343 VSSVYFDDDTLDVYRTRMERlEGATLVRVRWYGGgsstkdpspfaataegaegaegaegadddehGPSRVYVECKTHHES 422 Micromonas sp. ...
XP_001419043 169 ITSVYLDNPQFDVYRTRLAReQGATLARCRWYGGs-----------------------------aRSDDVFIERKTHHES 219 Ostreococcus lu...
Feature 1               #                                                                        
3G3R_A       102 WTGEKSVKARFALKERHVNDFLKGkytVDQVFAKMrkegkk------------pmneiENLEALASEIQYVMLK-KKLRP 168 baker's yeast
EEH57548     383 WSVDDSVKERFKLARRELAGFISG---VIDPSARFas--------------------dASARALATEISRDEIEaRRLTP 439 Micromonas pusi...
XP_002261454 351 WTGEISTKERFELQQKYVFKFMTGqmdVPKFFQRRrgtmgkegtqvkgtcvgnlekklKKNEKLAMEIQAMITK-NQLEP 429 Plasmodium know...
Q5CS43       392 WTGEKSAKERFSLPQKYVFGYLKGkfnVDSLIKEGifhtrkn----------qekenvEKSINLAKEIQNYIIN-NKLQP 460 Cryptosporidium...
Q8SRN4       267 WTGESSKKLRFMINEKQVLKYINGedvWEDVRRLNg---------------------dEDVRMLYEEVQSSIVN-RGLRP 324 Encephalitozoon...
XP_001827737 261 WTGEESKKLRFKIEEKYVNDFIQGkdvWKHVSRLNg----------------------KEIKYLYDEIQGAIVK-HTLKP 317 Enterocytozoon ...
EEH60426     410 WTGEESVKERFALPAAYIVPYLQCkhtWEKEESRLraqhakndr-----erptsgaelKKIKTLFTEVQRAIES-KQLQP 483 Micromonas pusi...
CAL54750     323 WSGEKSVKERVKISRATAVSIFDG---HTPLRDLP-----------------------ASDSDLINEVRSLEIDrRNIKP 376 Ostreococcus tauri
ACO63196     423 WSNESSVKERFRLDARDLKSYLAGg--VAAKSRTSdrtlh--------------qtldAKSATLAKEVAQDQLFaRGLAP 486 Micromonas sp. ...
XP_001419043 220 WSAQKSVKERCSMRREDAVVLLQGt--PPKALSMS-----------------------KKNLTLAREVLAQEIKrRGVKP 274 Ostreococcus lu...
Feature 1             # #               #                                                   # #  
3G3R_A       169 VVRSFYNRTAFQLPGDARVRISLDTELTMVREDNFDGVdrthknwrrt---digvdwpfkqLDDKDICRFPYAVLEVKLQ 245 baker's yeast
EEH57548     440 SLRVDYRRAAFQTASSNAIRVSLDTELAFTDARRRRPTrtttln------------pnisnPKAAAAVEFPHAVLEVKLH 507 Micromonas pusi...
XP_002261454 430 IIRTSYLRSAFQLSDDNSIRISIDTNVSMLNEYVRKREhwcrl--------------aeeaLRKNEIRRLNYAIIEVKLK 495 Plasmodium know...
Q5CS43       461 FVRTSYLRAAFQNQDNNQVRFSIDTNLCMVNEYISDGHqnepwc-----------rlaeevLSKNEVTRFPFAILEVKLQ 529 Cryptosporidium...
Q8SRN4       325 VVRTFYRRSAFQIPNDSSVRVSLDTDLCMIKECSDAEIvnptypitrwrrsdvncdfpfrnVPKSEIVRFPHAILEIKTQ 404 Encephalitozoon...
XP_001827737 318 KIRTFYKRISFQIPNDSTIRLSLDTNLCMIKENGENWRrtdimc-----------ewpfrqLEDTEIVRFPHAILEVKTQ 386 Enterocytozoon ...
EEH60426     484 TLRTVYMRTAFQVPYDASVRCSLDTSLAMLVENPAGGPsctalerw--------yrdpsvpLHRTEVTRFPHAVLEIKLA 555 Micromonas pusi...
CAL54750     377 VLMTKYRRTAFQRSDGNQIRISIDTELQWRDVRDLGTRais------------------ksSLHDFSPPFAYAVLEVKLS 438 Ostreococcus tauri
ACO63196     487 SVGTRYRRTAFQRADTNDVRVSLDVDLVFTDARDCSYPr---------------------dGSRRAPVRFPCAILEVKLA 545 Micromonas sp. ...
XP_001419043 275 TLLTQYRRTAFQRSDGNQLRVSFDTDLRWRDVRGEGSGvls-----------------naaFKGGEPAAFRFAILEVKLA 337 Ostreococcus lu...
Feature 1                                      ####                             
3G3R_A       246 tq----lgQEPPEWVRELVGSHL-VEPVPKFSKFIHGVATLLNdkv----------dsIPFWL 293 baker's yeast
EEH57548     508 e-------GEKCEWIDEALRAAPaSTEVYKFSKFLHGTVATRGgvndetpgggaflwrIPHWF 563 Micromonas pusilla CCMP1545
XP_002261454 496 v-------EKIPQWVQDILNSNH-FINAYKYSKYQTAMALLHPeki----------kyIPVWI 540 Plasmodium knowlesi strain H
Q5CS43       530 --------SEQPIWVSEMLKRCN-ATLVYKFSKFQHGMAILHEdkv----------siFPHWI 573 Cryptosporidium parvum
Q8SRN4       405 nf-----dETKPKWIEELINGPL-VEHVHKFSKYLHGCAVLYPfi-----------qdIPYWL 450 Encephalitozoon cuniculi
XP_001827737 387 gv-----dESKPTWLEDILNSTY-VEHVYKFSKFLHGIAILYNvk------------dIPYWL 431 Enterocytozoon bieneusi H348
EEH60426     556 lg----egVAAPDWVNELVASGA-LTEVHKFSKFMHGCAVLFPdta----------qeVPYWV 603 Micromonas pusilla CCMP1545
CAL54750     439 cs----eeEELMEWIDEVTSTFD-VIQVHKFSKFLHGCAMHFPrda---------lhtLPHWY 487 Ostreococcus tauri
ACO63196     546 radgddagGGTPDWIEDVLAAGN-ATEVKKFSKFLHATAALRVdtngg------vdaaLPHWY 601 Micromonas sp. RCC299
XP_001419043 338 te-----dAKSPEWIENIISSFN-VTHVYKFSKFLHGCAMLFYpnea--------lpkLPHWY 386 Ostreococcus lucimarinus CCE9901

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