3G3R,3G3O,3G3Q,3G3U,3G3T


Conserved Protein Domain Family
PolyPPase_VTC_like
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cd07750: PolyPPase_VTC_like 
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Polyphosphate(polyP) polymerase domain of yeast vacuolar transport chaperone (VTC) proteins VTC-2, -3 and- 4, and similar proteins.
Saccharomyces cerevisiae VTC-1, -2, -3, and -4 comprise the membrane-integral VTC complex. VTC-2, -3, and -4 contain polyP polymerase domains. For S. cerevisiae VTC4 it has been shown that this domain generates polyP from ATP by a phosphotransfer reaction releasing ADP. This activity is metal ion-dependent. The ATP gamma phosphate may be cleaved and then transferred to an acceptor phosphate to form polyP. PolyP is ubiquitous. In prokaryotes, it is a store of phosphate and energy. In eukaryotes, polyPs have roles in bone calcification, and osmoregulation, and in phosphate transport in the symbiosis of mycorrhizal fungi and plants. This subgroup belongs to the CYTH/triphosphate tunnel metalloenzyme (TTM)-like superfamily, whose enzymes have a unique active site located within an eight-stranded beta barrel.
Links
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Statistics
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PSSM-Id: 143622
Aligned: 73 rows
Threshold Bit Score: 115.875
Created: 16-Jun-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 14 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:based on Saccharomyces cerevisiae VTC4 polyP polymerase
  • Comment:This active site includes the substrate binding and acceptor pockets, and putative metal binding residues.
  • Structure:3G3R_A; Saccharomyces cerevisiae VTC4 polyP polymerase bound with a substrate analog (adenosine-5prime-[(beta,gamma)-imido] triphosphate) and Mn2+, contacts determined at 4.5A.
  • Structure:3G3T_A; Saccharomyces cerevisiae VTC4 polyP polymerase bound with orthophosphate, contacts determined at 4A.
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1           #                                        #                       # #        
3G3R_A       11 RQTTKYWVHPDNITELKLIILKHLPVLVFntnkeferedSAITSIYFDNEnlDLYYGRLRKd-EGAEAHRLRWYggm--- 86  baker's yeast
3G3Q_B       11 RQTTKYWVHPDNITELKLIILKHLPVLVFntnkeferedSAITSIYFDNEnlDLYYGRLRKd-EGAEAHRLRWYggm--- 86  baker's yeast
3G3U_A       11 RQTTKYWVHPDNITELKLIILKHLPVLVFntnkeferedSAITSIYFDNEnlDLYYGRLRKd-EGAEAHRLRWYggm--- 86  baker's yeast
3G3T_A       11 RQTTKYWVHPDNITELKLIILKHLPVLVFntnkeferedSAITSIYFDNEnlDLYYGRLRKd-EGAEAHRLRWYggm--- 86  baker's yeast
ZP_04450316  12 RIESKYLVDAKTMTALEKEFSQHLVPDDYp--------vSTISNLYFDNAdyDLVEDSLARl-HGREKVRMRTYdakp-- 80  Catonella morbi ...
ZP_03943437  46 RREYKYLLNAEEYITIRKLLMGHMSEDQYg--------lTTILSLYFDTPqyYFINQSMKKv-PYKSKFRVRSYgtp--- 113 Lactobacillus bu...
ZP_04061577  11 RIETKYVVSKENLEDLLTDLKEYLVEDDYp--------tSTISNIYFDTDnfDVIQDALDGn-HRQEKIRMRTYlanp-- 79  Streptococcus sa...
Q8DS84       12 RKEIKYLIEADTFEQLQKEFKDYLTPDRFt--------hSSITNIYFDNPdfQLIKDSINHr-HASEKIRMRTYdpkp-- 80  Streptococcus mu...
ZP_04355952  21 RIEKKYLVKRAIWPALSQALHEHLQEGPFg--------rGSVSSLYYDTPrnDMITASMGKp-FYKEKLRIRAYgep--- 88  Cryptobacterium ...
ZP_04499540   8 RKEVKYLLTLEQYETIRQGLVGHMQPDMYg--------rSLIQSVYLDTPd-RSIAVRYDGkqVYKEKLRVRRYvpansn 78  Slackia heliotri...

Feature 1                     #            #                                                    
3G3R_A       87 -----stDTIFVERKTHRedwtgekSVKARFALkerhvndFLKGkytvdqvfakmrkegkkpmn-----------eienl 150 baker's yeast
3G3Q_B       87 -----stDTIFVERKTHRedwtgekSVKARFALkerhvndFLKGkytvdqvfakmrkegkkpmn-----------eienl 150 baker's yeast
3G3U_A       87 -----stDTIFVERKTHRedwtgekSVKARFALkerhvndFLKGkytvdqvfakmrkegkkpmn-----------eienl 150 baker's yeast
3G3T_A       87 -----stDTIFVERKTHRedwtgekSVKARFALkerhvndFLKGkytvdqvfakmrkegkkpmn-----------eienl 150 baker's yeast
ZP_04450316  81 ----kadSFAFLEIKKKIle----vGHKYRFVAdmadiirYVAHptnarpdanpel------------------------ 128 Catonella morbi ...
ZP_03943437 114 ----lmdEPVFLELKKKIqg----vVYKRRLQIpydalenTLQTglscldhqqlvsne---------------------- 163 Lactobacillus bu...
ZP_04061577  80 ----kidSKVFLEVKAKDde---giGHKFRLVStplvitnLMTDgknhgqitdrqll----------------------- 129 Streptococcus sa...
Q8DS84       81 ----tqkSQAFLEIKKKEyqgeqeiGYKYRLTStplslcnYVQQgiidnnikqddra----------------------- 133 Streptococcus mu...
ZP_04355952  89 ----aadEAVFVELKTKVkg----sSYKRRVATscgaaqaWLAGmpyaeacarfplrstihrtassssmpshhpsesvsl 160 Cryptobacterium ...
ZP_04499540  79 vvpaadcDAVFVELKKKFkg----iTYKRRVHVnegvagaLMLAdksyldaldafmparsvlgep--------artaksv 146 Slackia heliotri...

Feature 1                              # #                            #                         
3G3R_A      151 ealaseiqyvmlkkKLRPVVRSFYNRTAFQlpg-------------daRVRISLDTELTMVREDnfdgvdrthknwrrtd 217 baker's yeast
3G3Q_B      151 ealaseiqyvmlkkKLRPVVRSFYNRTAFQlpg-------------daRVRISLDTELTMVREDnfdgvdrthknwrrtd 217 baker's yeast
3G3U_A      151 ealaseiqyvmlkkKLRPVVRSFYNRTAFQlpg-------------daRVRISLDTELTMVREDnfdgvdrthknwrrtd 217 baker's yeast
3G3T_A      151 ealaseiqyvmlkkKLRPVVRSFYNRTAFQlpg-------------daRVRISLDTELTMVREDnfdgvdrthknwrrtd 217 baker's yeast
ZP_04450316 129 --qltllelqrryvALKPMMFIGYKRHSYRgie-------------dpKVRVTIDREVVYRNYDldltagry-------- 185 Catonella morbi ...
ZP_03943437 164 nqikqeikwlfdryDIKPKVLISDDRVALStde-------------dpDFRVTFDFNIRFRDEDlslhhggy-------- 222 Lactobacillus bu...
ZP_04061577 130 --qdihhlrqrydeGLKPRMYIYYDRFSMKeketie-------gyaynKVRVTLDQNLTYRDEDvslfkgnh-------- 192 Streptococcus sa...
Q8DS84      134 --nhelailrerykKLVPKMFIHYERFSLRgie-------------npKLRVTFDRNVIYRHENvnltsgfn-------- 190 Streptococcus mu...
ZP_04355952 161 qivreidaarkrhaPLSPLIMTIVERVALTsas-------------rsDVRVTFDFDARWRMKDlsfdtgls-------- 219 Cryptobacterium ...
ZP_04499540 147 qiaselvatrdryeGLCPSMLINAVRVAWEpvgasdanihseetagigDVRITFDESIEYADLQhisreaaaqdfgs--- 223 Slackia heliotri...

Feature 1                              #                             #### 
3G3R_A      218 igvdwpfkqlddkdicrfPYAVLEVKLQtqlgqeppEWVRELVGSHlvepvpkFSKFI 275 baker's yeast
3G3Q_B      218 igvdwpfkqlddkdicrfPYAVLEVKLQtqlgqeppEWVRELVGSHlvepvpkFSKFI 275 baker's yeast
3G3U_A      218 igvdwpfkqlddkdicrfPYAVLEVKLQtqlgqeppEWVRELVGSHlvepvpkFSKFI 275 baker's yeast
3G3T_A      218 igvdwpfkqlddkdicrfPYAVLEVKLQtqlgqeppEWVRELVGSHlvepvpkFSKFI 275 baker's yeast
ZP_04450316 186 -----------geplvddDQVILEIKVNgd----qpDWMVEILNRYqve-eisFSKYG 227 Catonella morbi ATCC 51271
ZP_03943437 223 -----------gtkitpdFETIMEVKATga----ypVWFTDIIANLhlv-kgrFSKYG 264 Lactobacillus buchneri ATCC 11577
ZP_04061577 193 -----------gfplldeDTIIMEIKAPgk----kpDWLQTILDKHgle-eqkFSKYS 234 Streptococcus salivarius SK126
Q8DS84      191 -----------gypllknNQIVMEIKVKng----lpAWMEKIFHKHqli-pqsFSKYT 232 Streptococcus mutans
ZP_04355952 220 -----------gtplfadGCIIMEVKCQga----ypLWLAQLLSRSclr-pqpCSKVC 261 Cryptobacterium curtum DSM 15641
ZP_04499540 224 ------cfeapshrlledGLVLMEVKVLga----ypMWLSELLSSAkav-kssYTKYG 270 Slackia heliotrinireducens DSM 20476

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