2EWR


Conserved Protein Domain Family
NT_Pol-beta-like_1

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cd07749: NT_Pol-beta-like_1 
Click on image for an interactive view with Cn3D
Nucleotidyltransferase (NT) domain of an uncharacterized subgroup of the Pol beta-like NT superfamily.
The Pol beta-like NT superfamily includes DNA polymerase beta and other family X DNA Polymerases, as well as Class I and Class II CCA-adding enzymes, RelA- and SpoT-like ppGpp synthetases and hydrolases, 2'5'-oligoadenylate (2-5A)synthetases, Escherichia coli adenylyltransferase (GlnE), Escherichia coli uridylyl transferase (GlnD), poly(A) polymerases, terminal uridylyl transferases, Staphylococcus aureus kanamycin nucleotidyltransferase, and similar proteins. Proteins belonging to this subgroup are uncharacterized. In the majority of the Pol beta-like superfamily NTs, two carboxylates, Dx[D/E], together with a third more distal carboxylate, coordinate two divalent metal cations essential for catalysis. These divalent metal ions are involved in a two-metal ion mechanism of nucleotide addition. These carboxylate residues are conserved in this subgroup.
Statistics
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PSSM-Id: 143394
View PSSM: cd07749
Aligned: 4 rows
Threshold Bit Score: 207.414
Threshold Setting Gi: 91214520
Created: 13-Jul-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
metal binding
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1:metal binding triad [ion binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                # #                                      
2EWR_A         17 EYLRVLRKIYDRLKnekVNWVVTGSLSFALQGVp-VEVHDIDIQTDeEGAYEIERIFSefVSKKVRFSSTe--kICSHFG 93  Thermotoga mar...
NP_126101       5 AHLKVLRKLYERLKdsnVNWVVTGSLGFALQGVp-VEPHDIDIQTDkEGAYEIERLFSefVVEPVRFKESe--iIRSHFG 81  Pyrococcus aby...
jgi:Rxyl_0626   5 RHLRALRAVYEALSgreVRWALTGSLSFALQGAp-VEPDDIDIQTDrEGAYEIQRALHehVTRRVEFSSSaaggIRSHFG 83  Rubrobacter xy...
ZP_01251493     5 IYEEAFRYFLNKIIninIDWALTGSYRLYLENIhnFNPSDIDIITDnKGIELISSIFHknINTEFKYSESn--gIYSYFG 82  Psychroflexus ...
Feature 1                  #                                                                      
2EWR_A         94 ELIIDGIKVEIXGDIRKRLedGTWEDPVDLNk-YKRFVEthgXKIPVLSl--EYEYQAYLKLGR----VEKAETLRKWLN 166 Thermotoga mar...
NP_126101      82 VLTIDGIKVEIMGDIQKKVe-GKWEPPVDINr-YKRFVQiegMKIPVLDl--EYEYQAYLKLGR----IKKAEMLKKFLE 153 Pyrococcus aby...
jgi:Rxyl_0626  84 ELRIGGVRVEVMGDVQKRLpdGSWEAPVALEr-HTRRVEvegMSVPVLAl--EYEAAAYRRLGRldtaRMLEEMLEKENR 160 Rubrobacter xy...
ZP_01251493    83 QLQINNTTFDIMSDVKNRIegEWVETPNLSNieYIKFDN---YEIPVLSllsELELCNHLGLNQkknqIEEIIKIKKYAQ 159 Psychroflexus ...
Feature 1           
2EWR_A        167 ER 168 Thermotoga maritima
NP_126101     154 QR 155 Pyrococcus abyssi GE5
jgi:Rxyl_0626 161 ER 162 Rubrobacter xylanophilus DSM 9941
ZP_01251493   160 QS 161 Psychroflexus torquis ATCC 700755

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