2I7T,3IE0,2I7X,3AF6


Conserved Protein Domain Family
Int9-11_CPSF2-3-like_MBL-fold
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cd07734: Int9-11_CPSF2-3-like_MBL-fold 
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Int9, Int11, CPSF2, CPSF3 and related cleavage and polyadenylation specificity factors; MBL-fold metallo-hydrolase domain
CPSF3 (cleavage and polyadenylation specificity factor subunit 3; also known as cleavage and polyadenylation specificity factor 73 kDa subunit, CPSF-73) and CPSF2 (also known as cleavage and polyadenylation specificity factor 100 kDa subunit /CPSF-100) are components of the CPSF complex, which plays a role in 3' end processing of pre-mRNAs during cleavage/polyadenylation, and during processing of metazoan histone pre-mRNAs. CPSF3 functions as a 3' endonuclease. Int11 (also known as cleavage and polyadenylation-specific factor (CPSF) 3-like protein, and protein related to CPSF subunits of 68 kDa (RC-68)), and Int9, also known as protein related to CPSF subunits of 74 kDa (RC-74) are subunits of Integrator, a metazoan-specific multifunctional protein complex composed of 14 subunits. Integrator has been implicated in a variety of Pol II transcription events including 3' end processing of snRNA, transcription initiation, promoter-proximal pausing, termination of protein-coding transcripts, and in HVS pre-miRNA 3' end processing. Members of this subgroup belong to the MBL-fold metallo-hydrolase superfamily which is comprised mainly of hydrolytic enzymes which carry out a variety of biological functions.
Links
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Statistics
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PSSM-Id: 293820
Aligned: 8 rows
Threshold Bit Score: 213.347
Created: 9-Jun-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 7 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:based on Pyrococcus horikoshii CPSF Ph1404 and human CPSF3 (CPSF-73) the active site is located at the interface of the MBL-fold metallo-hydrolase and beta-CASP domains
  • Structure:3AF6: Pyrococcus horikoshii CPSF Ph1404 binds two zinc ions and a sulfate
  • Structure:2I7T: human CPSF3 binds two zinc ions and a sulfate
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1             #                                                                       
2I7T_A     15 RPLGAGQEVGRSCIILEfk-grKIMLDCGIHpgleg-------------------------------------------- 49  human
3IE0_A      4 VPFGAAREVTGSAHLLLag-grRVLLDCGMFqgkee-------------------------------------------- 38  Thermus thermophil...
Q5TA45      7 TPLGAGQDVGRSCILVSia-gkNVMLDCGMHmgfnddrr---------------------------------------fp 46  human
CBK22135    6 TVLGGGQSIGKSCFLLSvg-dqHVLLDCGSFvgkdt-------------------------------------------- 40  Blastocystis hominis
ELP93800    4 HVFGGGQSVGCSCFVVEgr-edAVVLDCGYNhvrsd-------------------------------------------- 38  Entamoeba invadens...
Q9NV88      2 KLYCLSGHPTLPCNVLKfk-stTIMLDCGLDmtstlnflplplvqsprlsnlpgwslkdgnafldkelkecsghvfvdsv 80  human
2I7X_A      4 KYNCCDDGSGTTVGSVVrfdnvTLLIDPGWNpskvs-------------------------------------------- 39  baker's yeast
3AF6_A    192 TGLGGFREVGRSALLVQtd-esFVLVDFGVNvamlndpyk------------------------------------afph 234 Pyrococcus horikos...

Feature 1                               # # ##                                                
2I7T_A     50 mdalpYIDLId-----pAEIDLLLISHFHLDHCGALPWFLqkt----sfkGRTFMThaTKAIYRWLLSDYVKvsnisadd 120 human
3IE0_A     39 -arnhAPFGFd-----pKEVDAVLLTHAHLDHVGRLPKLFreg-----yrGPVYATraTVLLMEIVLEDALKvmdepff- 106 Thermus thermophil...
Q5TA45     47 dfsyiTQNGRl-----tDFLDCVIISHFHLDHCGALPYFSemv----gydGPIYMThpTQAICPILLEDYRKiavdkkge 117 human
CBK22135   41 -kkalPDFSKlpksmtvNDITAVLISHFHMDHIGGLLYLTeql----kykGDIYASspTRAVLPLLLRNNRVlltnqaqv 115 Blastocystis hominis
ELP93800   39 igidfEKMNLl-----aPKIRCCCITHYHVDHVGMLPWLCkf------lsIPIFMAqpTLSVLPMILSDTHSsssfil-- 105 Entamoeba invadens...
Q9NV88     81 pefclPETELid----lSTVDVILISNYHCMMALPYITEHtg------ftGTVYATepTVQIGRLLMEELVNfiervpka 150 human
2I7X_A     40 yeqciKYWEKv-----iPEIDVIILSQPTIECLGAHSLLYynftshfisrIQVYATlpVINLGRVSTIDSYAsagvigpy 114 baker's yeast
3AF6_A    235 fdapeFQYVLr-----eGLLDAIIITHAHLDHCGMLPYLFryn----lfdGPIYTTppTRDLMVLLQKDFIEiqqsngqd 305 Pyrococcus horikos...

Feature 1                                                                           #         
2I7T_A    121 m-----------------------------lytetdleeSMDKIETINFHEVKEVa----gIKFWCYHAGHVLGAAMFMI 167 human
3IE0_A    107 --------------------------------gpedveeALGHLRPLEYGEWLRLg----aLSLAFGQAGHLPGSAFVVA 150 Thermus thermophil...
Q5TA45    118 an----------------------------fftsqmikdCMKKVVAVHLHQTVQVdd---eLEIKAYYAGHVLGAAMFQI 166 human
CBK22135  116 qs----------------------------tvtmetfakLTKHIREVTVNESIQLnp---nLRITAHIAGHVLGAVMWEI 164 Blastocystis hominis
ELP93800  106 ---------------------------------psdiekCVNQCSVIQPGQSIRItk---nVSLRCFYAGHALGAVCFEI 149 Entamoeba invadens...
Q9NV88    151 qsaslwknkdiqrllpsplkdavevstwrrcytmqevnsALSKIQLVGYSQKIELfg---aVQVTPLSSGYALGSSNWII 227 human
2I7X_A    115 dt---------------------------nkldledieiSFDHIVPLKYSQLVDLrsrydgLTLLAYNAGVCPGGSIWCI 167 baker's yeast
3AF6_A    306 p-----------------------------lyrprdikeVIKHTITLDYGEVRDIsp---dIRLTLHNAGHILGSAIVHL 353 Pyrococcus horikos...

Feature 1                  #                                       
2I7T_A    168 Eia--gVKLLYTGDFSRQEDRHl----mAAEIPNIk---------PDILIIES 205 human
3IE0_A    151 Qge--gRTLVYSGDLGNREKDVl----pDPSLPPL----------ADLVLAEG 187 Thermus thermophilus HB8
Q5TA45    167 Kvg--sESVVYTGDYNMTPDRHl----gAAWIDKCr---------PNLLITES 204 human
CBK22135  165 Eay--gRSILYTGDFSDEPGGFipsyqlPARFLRPg--------nLDMLIMEC 207 Blastocystis hominis
ELP93800  150 Lfd--gKTIVYTGDIDNQESFLi----rSAEITPHs---------INYLISES 187 Entamoeba invadens IP1
Q9NV88    228 Qsh--yEKVSYVSGSSLLTTHPq----pMDQASLKn---------SDVLVLTG 265 human
2I7X_A    168 Sty--sEKLVYAKRWNHTRDNIl----nAASILDAtgkplstlmrPSAIITTL 214 baker's yeast
3AF6_A    354 HignglHNIAITGDFKFIPTRLl----ePANAKFPr---------LETLVMES 393 Pyrococcus horikoshii OT3

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