2V9Q,2V9T


Conserved Protein Domain Family
IgC_1_Robo

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cd07693: IgC_1_Robo 
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First immunoglobulin (Ig)-like constant domain in Robo (roundabout) receptors, and similar domains
The members here are composed of the first immunoglobulin (Ig)-like domain in Roundabout (Robo) receptors. Robo receptors play a role in the development of the central nervous system (CNS), and are receptors of Slit protein. Slit is a repellant secreted by the neural cells in the midline. Slit acts through Robo to prevent most neurons from crossing the midline from either side. Three mammalian Robo homologs (Robo1, Robo2, and Robo3), and three mammalian Slit homologs (Slit1, Slit2, Slit3), have been identified. Commissural axons, which cross the midline, express low levels of Robo; longitudinal axons, which avoid the midline, express high levels of Robo. Robo1, Robo2, and Robo3 are expressed by commissural neurons in the vertebrate spinal cord and Slit1, Slit2,and Slit3 are expressed at the ventral midline. Robo3 is a divergent member of the Robo family which instead of being a positive regulator of Slit responsiveness, antagonizes Slit responsiveness in precrossing axons. The Slit-Robo interaction is mediated by the second leucine-rich repeat (LRR) domain of Slit and the two N-terminal Ig domains of Robo, Ig1 and Ig2. The primary Robo binding site for Slit2 has been shown by surface plasmon resonance experiments and mutational analysis to be is the Ig1 domain, while the Ig2 domain has been proposed to harbor a weak secondary binding site.
Statistics
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PSSM-Id: 409490
View PSSM: cd07693
Aligned: 6 rows
Threshold Bit Score: 173.507
Threshold Setting Gi: 115640628
Created: 7-Mar-2009
Updated: 17-Aug-2020
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 8 residues -Click on image for an interactive view with Cn3D
Feature 1:Slit binding interface [polypeptide binding site]
Evidence:
  • Structure:2V9T; human Robo domain Ig1 forms an interface with Slit protein second LRR (leucine rich repeat) domain, defined using 3.5 A contacts
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1          #  #        # # # #                                       ##                
2V9Q_A      10 PRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVetdkddprshRMLLPSGSLFFLRIVHGrksrpDEGVYVC 89   human
2V9T_A      10 PRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVetdkddprshRMLLPSGSLFFLRIVHGrksrpDEGVYVC 89   human
AAK94294    29 PRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVetdkddprshRMLLPSGSLFFLRIVHGrrskpDEGSYVC 108  chicken
NP_536792   91 PRIIEHPMDTTVPKNDPFTFNCQAEGNPTPTIQWFKDGRELktdt---gshRIMLPAGGLFFLKVIHSrr-esDAGTYWC 166  fruit fly
AAQ10890    71 PRIVHHPSDVVVRVGSPATLSCRAEGNPEPTIQWLRNGQPLdtdkmdaqsqPIVLPDGSLFFFSVVPGrkgqsHEAVYAC 150  zebrafish
XP_792790   22 PTITEQPKSLIVTRGDPASFNCKAEGRPEPTISWLKNGQPLvir----dtrHTVLDSGSLFFLQVVGNrqggqDGGVYQC 97   purple urchin
Feature 1                         
2V9Q_A      90 VARNYLGEAVSHDASLEVA 108  human
2V9T_A      90 VARNYLGEAVSHDASLEVA 108  human
AAK94294   109 VARNYLGEAVSRNASLEVA 127  chicken
NP_536792  167 EAKNEFGVARSRNATLQVA 185  fruit fly
AAQ10890   151 IAHNSIGNATSRNASLHIA 169  zebrafish
XP_792790   98 MAHNSLGTAYSANATLEIA 116  purple urchin

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