1I49,1I4T,1I4L,1I4D


Conserved Protein Domain Family
BAR_Arfaptin

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cd07660: BAR_Arfaptin 
Click on image for an interactive view with Cn3D
The Bin/Amphiphysin/Rvs (BAR) domain of Arfaptin
The BAR domain of Arfaptin-like proteins, also called the Arfaptin domain, is a dimerization and lipid binding module that can detect and drive membrane curvature. Arfaptins are ubiquitously expressed proteins implicated in mediating cross-talk between Rac, a member of the Rho family GTPases, and Arf (ADP-ribosylation factor) small GTPases. Arfaptins bind to GTP-bound Arf1, Arf5, and Arf6, with strongest binding to GTP-Arf1. Arfaptins also bind to Rac-GTP and Rac-GDP with similar affinities. The Arfs are thought to bind to the same surface as Rac, and their binding is mutually exclusive. Mammals contain at least two isoforms of Arfaptin. Arfaptin 1 has been shown to inhibit the activation of Arf-dependent phospholipase D (PLD) and the secretion of matrix metalloproteinase-9 (MMP-9), an enzyme implicated in cancer invasiveness and metastasis. Arfaptin 2 regulates the aggregation of the protein huntingtin, which is implicated in Huntington disease. Arfaptins are single-domain proteins with a BAR-like structure. BAR domains form dimers that bind to membranes, induce membrane bending and curvature, and may also be involved in protein-protein interactions.
Statistics
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PSSM-Id: 153344
View PSSM: cd07660
Aligned: 21 rows
Threshold Bit Score: 274.2
Threshold Setting Gi: 170591258
Created: 20-May-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
dimer interfaceRac binding
Conserved site includes 33 residues -Click on image for an interactive view with Cn3D
Feature 1:dimer interface [polypeptide binding site]
Evidence:
  • Structure:1I49; interface between monomers of human Arfaptin dimer; defined at 4A contacts.
    View structure with Cn3D
  • Structure:1I4D; interface between monomers of human Arfaptin dimer-Rac complex; defined at 4A contacts.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                        ##     #   #  #  ##  #  ##   # ##  #  ##  ## ##  #   #          
1I49_A         6 DLELELQIELLRETKRKYESVLQLGRALTAHLYSLLQTQHALGDAFADLSQKSPELqEEFGYNAETQKLLCKNGETLLGA 85  human
XP_002159700 159 DTELEKHIQALRETQLRYANLLKLSKQLTSQFSNLLVTQKLLGEALTDLSIKSPDLqDEFNQNAELQKIVSKSGDTLLSA 238 green hydra
NP_650058    140 DSELEAQIEQLRETQRKYLSILRLTRAFSSHFQHVVVTQHALADSFADLAQKNPELqKEFTCNSETQRNLTKNGELLLNA 219 fruit fly
EEC17508     165 DTELEAQIELLRDTQAKYLHVLRLARALASHFHQVVQTQGLLAEAFQELAHRSPPLqEEFRYNAEAQRSLGRNGETLLGA 244 black-legged tick
XP_001900387  35 DKELEEQIEQLKILHKYYNQVLTMSKLFMSNFHQMNEAQKSLAESLYQLSLKEMSLkAECSSNCDSLRSVAHNGELLERA 114 agent of lympha...
XP_002112284  27 DAQLEGQIQGLRDTQFKYTSLLRLIKQYIIQFRAVLITQASMGDTFADLAIRSVDLeSEFTSNAKTQKAILENGETMMAA 106 Trichoplax adha...
XP_001641868  33 DTELEAQILLLRETQAKYALMLRQSRQLMLQFQAMLQTQKSLGDTFSDLGMKSPELqDEFNYNAETQKSLIKNGEVLLGA 112 starlet sea ane...
CAF92520      16 DLDLESRLELLRHERQRYEQVGRLARTLAAQLAQFAATQRSLGDAFAELGLRTPPLhVEFSVNADAQKFLSKSGKTLADA 95  spotted green p...
AAW26366     105 DEELEDRISVFRNMHQQYRILHNLAEKLANSFRETIRHQRAFTNHMAALTVQQPELsSEFTYNTETQRIVISNGEKLLAS 184 Schistosoma jap...
XP_002234427 106 DLQLEAQIEVLRDTQRKYQQILRLARGMASHFYNVVQTQRALGDTFSDLAQKSPELqEEFTYNAETQKALCKNGETLLGA 185 Florida lancelet
Feature 1                                                                                        
1I49_A        86 VNFFVSSINTLVTKTMEDTLMTVKQYEAARLEYDAYRTDLEELSLGprd---------------------------agTR 138 human
XP_002159700 239 LNFFTENLQTLSGKTIDDTIMTIRDYESARIEYDAYRTDLEAYEAQg-------------------------------QS 287 green hydra
NP_650058    220 LNFFISSVNTLCNKTIDDTLLTIRQYETARIEFDAYRMDLENTKPElt-----------------------------pSA 270 fruit fly
EEC17508     245 LNFFVSSLATLCHKTIDDTLLTVRLYESARLEYDAYRTDLEELTQGtqg---------------------------naIS 297 black-legged tick
XP_001900387 115 LSFFLSSIKTLSEKTFEDTIETIHNYDQARLEYDVHRNEIVALQHSn------------------------------aSP 164 agent of lympha...
XP_002112284 107 MNFVVSSLTTLCDKTMADTLETVKVYENARIEYDAIRNEVESYPETn-------------------------------QS 155 Trichoplax adha...
XP_001641868 113 LSFFNTNLETLVHKTMEDSVTTVKAYESARIEYDAYRSDLEAAQAGprt---------------------------paTA 165 starlet sea ane...
CAF92520      96 IGAFTADMDTLINKTMEDTLVNARQYQAARVEYDAFRVDLEELNLRprd---------------------------agTL 148 spotted green p...
AAW26366     185 FDHFVKTLNTLTNHAFPDTWTTVASMYSARIEYDAYRNELNQVKNKkptsdniatttttttttnsgcstvlgtpvttdPI 264 Schistosoma jap...
XP_002234427 186 INFFTSNVNTLVNKTMEDTLLTIRQYEQARVEYDAYRTDLEQLQLGpkd---------------------------aaVQ 238 Florida lancelet
Feature 1                                          #   ##  ## ##      ## ##          
1I49_A       139 GRLESAQATFQAHRDKYEKLRGDVAIKLKFLEENKIkvMHKQLLLFHNAVSAYFAGNQKQLEQTLQQF 206 human
XP_002159700 288 FKTEEARKEFYIQRDKFESLRNNLQIKMKFLEENKVkvMRKQLLILHNAQAAYFSGNKEELEKTMKEF 355 green hydra
NP_650058    271 VALEETQRSYAQHKEQYEKLRSDVAVKMQFLDENRIkvMHKQLILLHNAIAAYFSGNAMALESTLKQF 338 fruit fly
EEC17508     298 SKMDEAQRNFALHKDKYEKLKADVTIKMKFLEENKVkvMHKQLLLFHNAVSAYFSGNQASLEATLKQF 365 black-legged tick
XP_001900387 165 EAIAGADFRCNQHRRKYEQLKADVKVKLRLLEENRWkvMRKQLLLLHNALIAYSSVAASVLKLSEKLP 232 agent of lymphatic filariasis
XP_002112284 156 PRAIALRHEFGSAKEKFEKLRNDVSVKLKFLEENKAkvMHKQLVLFHDAFCAYFSGNQEALQTIVRDF 223 Trichoplax adhaerens
XP_001641868 166 ARAEETREQFEKQKAKFDRLRNDLTIKLRFLDENKVkvMHKQLLLFNNAITAYFTGNKQALETCIKEF 233 starlet sea anemone
CAF92520     149 ARLERAQRDFQDQRDRYQRVRRDLSVKVRLLEHNKVkvLHQQLWLLHGAMAAHSLSCHAHLEQSLQQA 216 spotted green pufferfish
AAW26366     265 HDKHNLQSRLDAASERYSQLKKVVSCKLEFLQENRLrvMQHQLVLLQNATSAFFSGNNQQLDQILNQY 332 Schistosoma japonicum
XP_002234427 239 ARMKDADSNFQAHKDKFDRLRGDVAVKLKFLEENKVkvMHKQLLLFHNAISAYFAGNQTALEATLKQF 306 Florida lancelet

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