2VHI,2VHI


Conserved Protein Domain Family
ML_beta-AS

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cd07587: ML_beta-AS 
Click on image for an interactive view with Cn3D
mammalian-like beta-alanine synthase (beta-AS) and similar proteins (class 5 nitrilases)
This subgroup includes mammalian-like beta-AS (EC 3.5.1.6, also known as beta-ureidopropionase or N-carbamoyl-beta-alanine amidohydrolase). This enzyme catalyzes the third and final step in the catabolic pyrimidine catabolic pathway responsible for the degradation of uracil and thymine, the hydrolysis of N-carbamyl-beta-alanine and N-carbamyl-beta-aminoisobutyrate to the beta-amino acids, beta-alanine and beta-aminoisobutyrate respectively. This subgroup belongs to a larger nitrilase superfamily comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes, which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13), this subgroup corresponds to class 5. Members of this superfamily generally form homomeric complexes, the basic building block of which is a homodimer. Beta-ASs from this subgroup are found in various oligomeric states, dimer (human), hexamer (calf liver), decamer (Arabidopsis and Zea mays), and in the case of Drosophila melanogaster beta-AS, as a homooctamer assembled as a left-handed helical turn, with the possibility of higher order oligomers formed by adding dimers at either end. Rat beta-AS changes its oligomeric state (hexamer, trimer, dodecamer) in response to allosteric effectors. Eukaryotic Saccharomyces kluyveri beta-AS belongs to a different superfamily.
Statistics
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PSSM-Id: 143611
View PSSM: cd07587
Aligned: 18 rows
Threshold Bit Score: 749.194
Threshold Setting Gi: 30698009
Created: 21-Apr-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 9 residues -Click on image for an interactive view with Cn3D
Feature 1:putative active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                         
2VHI_C          9 LNDCLEKHLPPDELKEVKRILYg---vEEDQTLELPTSAKDIAEQNGFDIKGYRFTAREeqtrKRRIVRVGAIQNSIVIP 85   fruit fly
CAQ13724        9 LEKVLEAHLPEAELKEARRLLFg----KELKKLDLPRSAIDAAGQQDFDLKGYVFEASPeqlrPPRMVRVGLIQNKIVLP 84   zebrafish
EEC14098       10 VEKILKEHLPAEEHEKVRAVLYg----RDCSPLEFSSDALGQAKQHKFELKGYKMAAEReelrPARVVRVGLVQNKIVLP 85   black-legged ...
XP_002113272    8 LESLLEKNLQGRDLDEAKRILYg----SGFSALTLPETASREATDLDFELKGYKISAQAeetrPPRIVRIGLVQNKIHLP 83   Trichoplax ad...
Q964D8          8 VQATLEKYIPAEELSEVKRILYgynrgHHVKSLPICQEALDLANKNNFEIVASKVEADPeqlrKPRIVRLGIIQNSIGAE 87   Dictyostelium...
ABR17023       43 LHDLLQSNLPPSLYKEVSQLLLgvncgKVVSPLSLPETVLARSKESSFDLQAYRFNAESemmrAPRIVRVGLIQNSIVLP 122  Sitka spruce
NP_201242      23 LHQLLSANLKPELYQEVNRLLLgrncgRSLEQIVLPESAKALSSKHDFDLQAASFSADKeqmrNPRVVRVGLIQNSIALP 102  thale cress
XP_001767736   20 LHRLLSSELPPHLFQEVSRVLYgcnrgSAVTPVPQTEDLSAAAKEGNFDVQTFEFTADLeqmrAPRNVCVGLIQNAIVLP 99   Physcomitrell...
XP_001014048  623 VEQAFDTYIPEKVRSEVYRILYg----QNYKPLDLALRTHEMALEHNFEIKGFRVQANKeqtrPPRLVRIGAVQNIYKAS 698  Tetrahymena t...
XP_002238142    8 VDDVLREHIPPEKLGEVQRVLYg----SSFSKLNLPAEAVKLANERKFELAGYGMQAVPeqlrPARLVRVGAVQNKIVLP 83   Florida lancelet
Feature 1                                           #                                             
2VHI_C         86 TTaPIEKQREAIWNKVKTMIKAAAeAGCNIVCTQEAWTMPFAFCTREKfPWCEFAEEaenGPTTKMLAELAKAYNMVIIH 165  fruit fly
CAQ13724       85 TDaPVLEQITALHKRVGEMVEVAAmCGVNIVCFQEAWTMPFAFCTRERePWTEFAESaedGLTTRFCIQLAKKHNMVVVS 164  zebrafish
EEC14098       86 TTeTVTAQREALHKRIETIVDTAAlCGVNIICFQETWHMPFAFCTREKtPWCEFAESaehGPTVQLCQQMAKKHNMVVVC 165  black-legged ...
XP_002113272   84 TTaSIVEQREALHQRISQITKVAAdCGVNVICYQEAWNMPFAFCTREKhPWCQFAESaedGPSAKLCCQLAERYNMVILS 163  Trichoplax ad...
Q964D8         88 TTaPIQDQYLAIEAKIEKMIDAAGaMGVNVLCLQETWHMPFAFCTREKyPWVEFAESastGQSIKFIQRMARKYNMVIIS 167  Dictyostelium...
ABR17023      123 TTaPFSEQRKAIMQKVKCMIEAAGvAGVNIVCLQEAWTMPFAFCTREK-RWCEFAESv-dGESTQFLKELAQKYNMVIIS 200  Sitka spruce
NP_201242     103 TTaPFSDQTRGIFDKLKPIIDAAGvAGVNILCLQEAWTMPFAFCTRER-RWCEFAEPv-dGESTKFLQELAKKYNMVIVS 180  thale cress
XP_001767736  100 TTePFLKQKHAIMERVTSLIESAAkAGVNILCLQEAWTMPFGFCTREK-RWCEFAESaeaGESTKLLQGLAQRYNMVIVS 178  Physcomitrell...
XP_001014048  699 PSqPIQEQRQAIHDYNKKVVEAAYhSGVNVICFQELWTCPFFVATREKyPWIELAESaehGPTTQMLQELARKYNMVIVN 778  Tetrahymena t...
XP_002238142   84 TSaPIAEQVAALHSRIAEIVEVAAmCGVNIICFQETWTMPFAFCTREKqPWCEFSESaedGPTITFCQELAKKYNMVIIS 163  Florida lancelet
Feature 1                                        #   #      #                         ## ##       
2VHI_C        166 SILERDmehgETIWNTAVVISNSGRYLGKHRKNHIPRVGDFNESTYYMEGNTGHPVFETeFGKLAVNICYGRHHPQNWMM 245  fruit fly
CAQ13724      165 PILERDeihgGTLWNTAVVVSNNGNVLGKTRKNHIPRVGDFNESTYYMEGNTGHRVFQTqFGKIAVNICYGRHHPLNWLM 244  zebrafish
EEC14098      166 PILERDe--nDVMWNTAVVVSNSGAILGKSRKNHIPRVGDFNESTYYMESKLGHPVFQTqFGKIAINICYGRHHPLNWFM 243  black-legged ...
XP_002113272  164 PILERDslhgDTIFNTTTVISNHGKVIGKTRKNHIPRVGAFNESTYYMEGDTGHRVFETdFGKIAINICYGRHHPLNWLM 243  Trichoplax ad...
Q964D8        168 PMLERDdvhaSTIHNTAVVVGNNGNIIGKSRKNHIPRTGDFNESTYYMESTLGHPVFETiYGKIAINICYGRHHNLNWLA 247  Dictyostelium...
ABR17023      201 PILERDmthgEVIWNTAIVIGNHGNIIGKHRKNHIPRVGDFNESTYYMEGNTGHPVFETcYGKIGINICYGRHHPLNWLA 280  Sitka spruce
NP_201242     181 PILERDidhgEVLWNTAVIIGNNGNIIGKHRKNHIPRVGDFNESTYYMEGDTGHPVFETvFGKIAVNICYGRHHPLNWLA 260  thale cress
XP_001767736  179 PILERDeshgSTIWNTAVVIGNHGNVIGKHRKNHIPRVGDFNESTYYMEGNTGHPVFETeFGKIAVNICYGRHHPMNWQA 258  Physcomitrell...
XP_001014048  779 SILERDde-rGTIYNTTVVISSKGKFLGKHRKNHIPRVGDFNESTYYMEGNTGHPVFETeFGKIGVNICYDRHHPLSWQQ 857  Tetrahymena t...
XP_002238142  164 PILERDaahgDIIANTAVVISNTGTVLGKSRKNHIPRVGDFNESTYYMEGNTGHRVFQTqFGKIAINICFGRHHPLNWMM 243  Florida lancelet
Feature 1                      #                                                                  
2VHI_C        246 FGLNGAEIVFNPSATIGrLSEPLWSIEARNAAIANSYFTVPINRVGTEQFPNEYTSGDGNKAHKEFGPFYGSSYVAAPDG 325  fruit fly
CAQ13724      245 YSVNGAEIIFNPSATVGlLSEPMWPIEARNAAIANHCFTCAINRVGTEYFKNEFTSGDGKKAHHDFGHFYGSSYMAAPDG 324  zebrafish
EEC14098      244 YAANGADIVFNPSATVAgLSEPLWHVEARNAAIANSYFTCAINRVGTEVFPNEFTSGNKKGAHKDFGHFYGSSYVAAPDG 323  black-legged ...
XP_002113272  244 YGVNGAEIVFNPSATVGaLSEPMWSIEARNAAIANTYFTAGINRVGTESYPNEFTDGDGKPAHHDFGHFYGSSYVAAPNG 323  Trichoplax ad...
Q964D8        248 YGLNGAEIVFNPSATVGeLSEPMWGVEARNAAMTNNYFVGSINRVGTEHFPNEFTSGNGKPAHKDFGHFYGSSYFSSPDN 327  Dictyostelium...
ABR17023      281 FGLNGAEIVFNPSATVGgLSEPMWSIEARNAAIANTFFVGSINRVGTEVFPNPFTSGDGKPQHADFGHFYGSSYFSAPDA 360  Sitka spruce
NP_201242     261 FGLNGAEIVFNPSATVGeLSEPMWPIEARNAAIANSYFVGSINRVGTEVFPNPFTSGDGKPQHNDFGHFYGSSHFSAPDA 340  thale cress
XP_001767736  259 FGMNGAEIVFNPSATVGdLSEPMWPIEARNAAIANSYFVGAINRVGTEVFPNEFTSGDGKPAHKDFGHFYGSSFVAAPDA 338  Physcomitrell...
XP_001014048  858 LGLNGAEIVFNPSATVGgLSEPMWPIEARNAAIANHYFSVGINRVGTEIFPNKFTSGDGGQARNDFGHFYGSSYVAAPDG 937  Tetrahymena t...
XP_002238142  244 YGINGAEIVFNPSATVGaLSEPMWPIEARNAAIANSYFSVAINRVGTESFQSEFTSGDGKKAHHDFGHFYGSSYLAAPDG 323  Florida lancelet
Feature 1                                                        
2VHI_C        326 SRTPSLSRdKDGLLVVELDLNLCRQVKDFWGFRMTQRVPLYAESFKK 372  fruit fly
CAQ13724      325 SRTPGLSRtRDGLLVAELDLNLNRQVADKWNFKMTGRYEMYAEELKK 371  zebrafish
EEC14098      324 SRTPGLSRtQDGLLVTELDFNLCRQVRDSWGFRMTQRTEIYADALYW 370  black-legged tick
XP_002113272  324 GRTPGLSRvNEGLLVVELDLNLIRQIKDKWGFQMTARLDMYAKELAN 370  Trichoplax adhaerens
Q964D8        328 CCTPSLSRvSDGLNISEVDLNLCQQVKDKWNFQMTARYELYAKFLTD 374  Dictyostelium discoideum AX4
ABR17023      361 SCTPSLSRyQDGLIIADMDLNLCRQVNDKWAFRMTARYDMYAELFAS 407  Sitka spruce
NP_201242     341 SCTPSLSRyKDGLLISDMDLNLCRQYKDKWGFRMTARYEVYADLLAK 387  thale cress
XP_001767736  339 SCSPSLSRcRDGLLVAKLDLNLCQQLKDKWGFRMTSRIDMYADFFRE 385  Physcomitrella patens subsp. patens
XP_001014048  938 SRTPGLSRtRDGLLVVEVDLNLCQQIKDKWGFTMTGRHEYYAEKLTD 984  Tetrahymena thermophila SB210
XP_002238142  324 SRTPGLSRvDDGLLVTEVDLNLCRQVKDKWCFRMTARLDMYAESLAY 370  Florida lancelet

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