3HKX


Conserved Protein Domain Family
R-amidase_like

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cd07576: R-amidase_like 
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Pseudomonas sp. MCI3434 R-amidase and related proteins (putative class 13 nitrilases)
Pseudomonas sp. MCI3434 R-amidase hydrolyzes (R,S)-piperazine-2-tert-butylcarboxamide to form (R)-piperazine-2-carboxylic acid. It does so with strict R-stereoselectively. Its preferred substrates are carboxamide compounds which have the amino or imino group connected to their beta- or gamma-carbon. This subgroup belongs to a larger nitrilase superfamily comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes, which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13), class 13 represents proteins that at the time were difficult to place in a distinct similarity group. It has been suggested that this subgroup represents a new class. Members of the nitrilase superfamily generally form homomeric complexes, the basic building block of which is a homodimer. Native R-amidase however appears to be a monomer.
Statistics
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PSSM-Id: 143600
View PSSM: cd07576
Aligned: 37 rows
Threshold Bit Score: 265.983
Threshold Setting Gi: 118472869
Created: 21-Apr-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 9 residues -Click on image for an interactive view with Cn3D
Feature 1:putative active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                   #                                    
3HKX_A        22 RIALMQHTar----pLDPQHNLDLIDDAAARASEqgAQLLLTPELFGFGYvpsqicaqv-saeqvdaaRSRLRGIARDRG 96  Nesterenkonia s...
ZP_01086170   28 RLGLWQGAgsa-gtpEAVIENLERLEAVTALAASnqVQLLAFPELYLSGYalsheaawrlaephdgpsLRRVAAAARRHG 106 Synechococcus s...
ZP_00960698    4 TLAIAQPPgd----lGLPEERLGWLRDHLATLQA--TDLLLLPELFHCGYnigdalper-aeprdgpfARAMADLARQHG 76  Roseovarius nub...
ZP_01752930    6 NIVVGQSPad----lDGPQARLTWLRECLSQLDGqhVDLLLLPELFLTGYnigsrvtdr-aepadgpsAQAIAELARAHR 80  Roseobacter sp....
YP_001777942   5 RLRLIQSTvk----dGAHASNLAQALAHIAAARGn-ADLVIFSETYVSGFptaenvahl-aepldgpsVSAIRAAARDAH 78  Burkholderia ce...
ZP_03493080   13 RVAVAQIAia----dGDVEANLDKVAQVLCEASArrADFLLLPELCLTGLvagdalvrl-aepphgpmQQAMRDLARHHG 87  Alicyclobacillu...
YP_001358191  64 RLGIYQAQavs--geGATAKNLKRMEHAIRLAKEkhIQLLSFPELYIPGYtlspamvkkvaqfkdgpaVTKARELARRNN 141 Sulfurovum sp. ...
YP_890941      5 DVAVVQEPav----aGDVAANVRRAVAALAKHPG--ADLVVFPELFLCGYrldvvadaaiemipepgpVADLCAAAAAHD 78  Mycobacterium s...
NP_440387     19 RLAIYQNQggpigntEAIAYYLAKMEEALQGAQRfeAQLISFAELYLTGYalspqevhqlaiardgevMTQVGQLAHKYQ 98  Synechocystis s...
YP_001584980   5 VVRLFQSTcv----dGDVAHNLDAALRAIADSTGr-ADLIVFPETFIPGFptpenvakl-aepidgtsISAIRAAARAAR 78  Burkholderia mu...
Feature 1                                              #   #   #                                #
3HKX_A        97 IALVWSLPGPegp----eqRGITAELADEHGevLASYQKVQLYgpEEKAAFVPGEq-------pPPVLSWgGRQLSLLVC 165 Nesterenkonia s...
ZP_01086170  107 VAIACPYPERavva-gcecLYDAIALFDQDGtlLRNYRKTHLWgpDEALLWTAGYrepeegpayTVQRVN-GLPLGLLNC 184 Synechococcus s...
ZP_00960698   77 VALAYGYAEAae-----grLYNSAQCLDKSGqpIGHHRKLTLPpgYEADHFTRGAd-------lGQFTLG-GLTCGFLIC 143 Roseovarius nub...
ZP_01752930   81 IAIHYGFAERqd-----gqIFNSASCISKDGtlLATHRKLLLPpgFEGDHFCPGIg-------yTQFELN-GFNVATLIC 147 Roseobacter sp....
YP_001777942  79 VAVVIGVAEQdd-----grYFNTAILVDEFGelRLRYRKSHLYe-SDVGVFEAGGt-------fDVCEWR-GVKVGMLIC 144 Burkholderia ce...
ZP_03493080   88 VATAFSYPEQred----grLHIATQVVDRDGrsLHVYRKTHLFa-EEKVSYQPGDa-------iAPFSLW-DWPSGLLTC 154 Alicyclobacillu...
YP_001358191 142 IAILLPYAEKakhsdgtlaYYDSIAVIDEHGklLNSYRKTHLYgqQERDNWSFGNgd------yQVYHFF-GFPVGVLNC 214 Sulfurovum sp. ...
YP_890941     79 TAVVTGFAERsg-----dlVYNSLLCIDRTGavAGVYRKTHLFg-AECEAFATGDr-------lEVIEVD-GLRVGPMIC 144 Mycobacterium s...
NP_440387     99 MAIICPYPEKaain-geihYYDSINLFDDQGklVKTYRKTHLWgpDESKIYSRGHrhkeegkafTVHKVN-GFPIGLLNC 176 Synechocystis s...
YP_001584980  79 VSVAIGFAESdg-----ghYYNTALLIDEHGhiLLKYRKTHLYa-SDIGVFERGQt-------fPVCEWH-GIRVGMLIC 144 Burkholderia mu...
Feature 1        # ##                    #                                                       
3HKX_A       166 YDVEFPEmVRAAAaRGAQLVLVPTALAGDet------------sVPGILLPARAVENGITLAYANHCGPEGGLv------ 227 Nesterenkonia s...
ZP_01086170  185 YEGEFPElTRLLVlAGARLVLIPTAADTWmllsdgrrtdrpypdVSRTLLPAHAFSSQCFVAYANRCGEESVGeqv---- 260 Synechococcus s...
ZP_00960698  144 YDVEFPEnTRAQAmAGCDLILVPTALGADwg------------vVSRHLIPTRAFENGAYVAYADHAGAQNGHg------ 205 Roseovarius nub...
ZP_01752930  148 YDAEFPEtFRAVAqAGAELVLVPTALGAQwg------------vVANTVIPARAFENGIYVCYANSCGHENGMd------ 209 Roseobacter sp....
YP_001777942 145 FDLEFPEtARALArAGAELIVIPDGMMQPhg------------hVHRKMIPVRALENQVFVAMANRVGPGDRYt------ 206 Burkholderia ce...
ZP_03493080  155 YDVEFPEpARQLAlAGCRLLFVNAANMEPye------------gVHRTFVAARAMENQIFVVYCNRVGANARYr------ 216 Alicyclobacillu...
YP_001358191 215 YECEFPElSRILAlKGAKLIVGPTAADNYytlpdgkrsnvpypdISKTLIPAYAYANNIFFAYSNRAGYERRGkdq---- 290 Sulfurovum sp. ...
YP_890941    145 FDVEFPEiARTLAlSGVDLFVVSSANMFPyh------------hDHRVACLSRALENRTPLVYSNLIGAEAGLt------ 206 Mycobacterium s...
NP_440387    177 YEAEFAElTRILAlRGAKLVVIPTAADIWtllstgertkipypdVSHNVIPVRALENHIFVAYCNRAGAETRLnaqgktv 256 Synechocystis s...
YP_001584980 145 FDIEFPEtARILArNGAELMLVLDGLMCPyg------------nVHRTMLPVRAMENQAYMVLVNRAGPGDRYt------ 206 Burkholderia mu...
Feature 1                                                                   
3HKX_A       228 ----FDGGSVVVGPAGQPLGELGve-pGLLVVDLPDQ--SQDAGSds--adYLQDRRAE 277 Nesterenkonia sp. 10004
ZP_01086170  261 -rgaYLGNSVVAAPDGSLLLAASde-pTLLIADCRPG--DYGPSHpeg-ttYLSDRRTD 314 Synechococcus sp. WH 5701
ZP_00960698  206 ----YFGGSVIADPWGTPLARAAqt-pDIIRATLDPA--QVAEARar--lpYLRDRAAL 255 Roseovarius nubinhibens ISM
ZP_01752930  210 ----FYGGSCVIAPDGQELARAGdg-eELLRAQANLEavAVAQARl----pYLADRLNL 259 Roseobacter sp. SK209-2-6
YP_001777942 207 ----FSGESIVASPEGDVIAAAPs--dRDAVVDVDID--MDAVSRgraafrYLDRVHPA 257 Burkholderia cenocepacia MC0-3
ZP_03493080  217 ----YRGNSSVVAPDGTVLLDLGldqeAVQCVDLEWQ--AIVQARva--ydYLAERRFL 267 Alicyclobacillus acidocaldarius LAA1
YP_001358191 291 --whYRGNSIITGPHGDIIVAANheqdTMLIADCIPG--FYGMTHpapkynYLRDRRPE 345 Sulfurovum sp. NBC37-1
YP_890941    207 ----FVGGSRVVDAGGELLVECGg---EVSVARCRVT--PATATSteddvdYLRHRRPE 256 Mycobacterium smegmatis str. MC2 155
NP_440387    257 lvgeYLGNSAIAGPHGDLLLYPRne-eTLLIADCVSA--DYGSLHpeq-tnYLVDRQSN 311 Synechocystis sp. PCC 6803
YP_001584980 207 ----FAGQSHVADPFGCSVFVADaeraDPQDVRLDMS--AVARSRea--fsYVELTAIT 257 Burkholderia multivorans ATCC 17616

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