Conserved Protein Domain Family
CPA

?
cd07573: CPA 
N-carbamoylputrescine amidohydrolase (CPA) (class 11 nitrilases)
CPA (EC 3.5.1.53, also known as N-carbamoylputrescine amidase and carbamoylputrescine hydrolase) converts N-carbamoylputrescine to putrescine, a step in polyamine biosynthesis in plants and bacteria. This subgroup includes Arabidopsis thaliana CPA, also known as nitrilase-like 1 (NLP1), and Pseudomonas aeruginosa AguB. This subgroup belongs to a larger nitrilase superfamily comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes, which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13), this subgroup corresponds to class 11. Members of this superfamily generally form homomeric complexes, the basic building block of which is a homodimer; P. aeruginosa AugB is a homohexamer, Arabidopsis thaliana NLP1 is a homooctomer.
Statistics
?
PSSM-Id: 143597
View PSSM: cd07573
Aligned: 47 rows
Threshold Bit Score: 377.673
Threshold Setting Gi: 219677880
Created: 21-Apr-2009
Updated: 2-Oct-2020
Structure
?
Aligned Rows:
 
Feature 1: putative active site
Evidence:

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1                                               #                                        
XP_001755668   7 VGVAALQFAcsddVESNVSKAETLVRDAhaqGANIVLIQELFEGYYFCQAQREDYFarshp--reghPTIERMQRLAKEL 84  Physcomitrella ...
YP_001825000  24 LRVAAVQQRwhrdPAEHRAALLEGIRLAaaeGARVVCLQELTLSPYFAVVRRADHPepaapeelltgPTFTFAAGAAREH 103 Streptomyces gr...
EED34229      22 LTIALYQMRwypdPEAHKRRIEEGVAAAamaGAQIVFLPELTLSRYPADTLPEGRAdttae-pladgPTFLFAAELAKRY 100 gamma proteobac...
ACH68605       1 MTVAATQMScg-nPEENIKKAESLVRIAssrGAQVTLLQELFQFSYFPIELNAGNFqlatt--leesALVQGMALLAKEL 77  Phytophthora sojae
YP_331750      4 TTVAVTQMAcdwnVDANLSRAERLVRDAaarGAQIVLLQELFETPYFCIDQHPAHLalaqp--yeghPWLMRFASLAREL 81  Burkholderia ps...
XP_001690094  10 VVLAVTQFGmsedRAANADKAEQYVRRAaaaGGQIVVLPELFESMYFPMVHSEEYFqlaap--feghPLVERFARLAAEL 87  Chlamydomonas r...
NP_345406      4 VRVATIQMQcakdVATNIQTAERLVRQAaeqGAQIILLPELFEHPYFCQERQYDYYqyaqs--vaenTAIQHFKVIAKEL 81  Streptococcus p...
ZP_03461426    4 VCVGAVQMRcskdTAANIEKADSLVREAaagGAQIILLPELFENLYFCQERNYDYYslatt--pdenPAVAHFKKVAEEL 81  Bacteroides pec...
ZP_01078488    4 IKVAATQMVctwdTANNIQRAEEQVRKAhqqGAQIILLQELFETPYFCQEVDAKHFklakp--lngnKFIEHFQGLAREL 81  Marinomonas sp....
XP_001692986   5 VMLAATQFEctadKAANADKAEELVRKAaaaGANIILLQELFHGLYWCAVQDPAFLswaap--leghPLLARFGALAAEL 82  Chlamydomonas r...
Feature 1                                                 #   #      #                           
XP_001755668  85 GVVIPVSFFEEAnn-------aHYNSIVVIDADGTdLGVYRKSHIPDGPGYQEKFYFNPGDt---GFKVFKTk----FAT 150 Physcomitrella ...
YP_001825000 104 GVYVHASLYEKApapggeddglGYNTAILVAPDGTlAQRTRKTHIPVTEGYYEDDWFRAGPagddAFPLVEVd----EAR 179 Streptomyces gr...
EED34229     101 DLHVHASLFQLSgad----dgrGLNTAIVVSPNGElIAHTHKLHIPVTTGYYEDHYFAPGPne-aPYPVHHMafdsgDAA 175 gamma proteobac...
ACH68605      78 HVVIPISFFERYkn-------sYYNSCAVIDADGTvLGVTRKMHIGDRLGYNEKYYFTPSDd---SFKVWNTr----YGK 143 Phytophthora sojae
YP_331750     82 GVVLPVSFFERAgq-------tQFNSVAIFDADGRaLGIYRKTHIPDGPGYTEKYYFTPGDt---GFRVWDTa----YGR 147 Burkholderia ps...
XP_001690094  88 RVVLTVSFFEVAnt-------tYFNSCALVDADGTvLGRYRKSHIPDGPGIRWKFYMSPGDt---GLDVYDTa----YGR 153 Chlamydomonas r...
NP_345406     82 QVVLPISFYEKDgn-------vLYNSIAVIDADGEvLGVYRKTHIPDDHYYQEKFYFTPGNt---GFKVWNTr----YAK 147 Streptococcus p...
ZP_03461426   82 RVVLPVSFYEKAgn-------tAFNSIAIIDADGSiLGVYRKTHIPDDHYYQEKFYFSPGDt---GFKVWDTa----YAR 147 Bacteroides pec...
ZP_01078488   82 EVALPISFFEIDgn-------nYFNSIVVLDADGAiVSHYRKVHIPDAVGYYEKYYFSPGDt---GFQVAKTr----YAD 147 Marinomonas sp....
XP_001692986  83 GVVLPVPFFERHnn-------aHFNSVAVMDADGScKGVYRKSHIPDGPGYTEKFYFNPGDt---GFRVFDTk----YGR 148 Chlamydomonas r...
Feature 1             ## ##                    #                                                 
XP_001755668 151 IGVGICWDQWFPEAARAMALMGAEVLFYPTAIGSEPqdgdldssEHWRRVMQGHAGANLVPLVASNRIGQEiiete---- 226 Physcomitrella ...
YP_001825000 180 LGLPTCWDQWFPELARAYSLAGADVLVYPTAIGSEPgfpgfdsqPLWQKVITGNAIANATFMVVPNRIGAEg-------- 251 Streptomyces gr...
EED34229     176 LGLPTCWDEWFPEVARNYALRGAEMLAYPTAIGSEPdfpdfdtePLWRQTIVGHAIANGLFIAVPNRFGGEg-------- 247 gamma proteobac...
ACH68605     144 IGVAIGSDQWYPEVARSLVVQGAELLLYPSAMGSNQydpnfdprDQWQRVMQGHAAANMVPVICSNRVGSEvvd------ 217 Phytophthora sojae
YP_331750    148 IGVGICWDQWFPECARAMALAGAELLLYPSAIGSEPhdasidsrAHWHNAQRGHAAANLMPVAASNRVGVErga------ 221 Burkholderia ps...
XP_001690094 154 VGLAICWDQWFPEAARALVLQGAEVLVYPSCIGDEPhdpandsyPHWMRVQQGHAAANMVPLLAANRVGREqlqg----- 228 Chlamydomonas r...
NP_345406    148 IGIGICWDQWFPETARCLALNGAELLFYPTAIGSEPildt-dscGHWQRTMQGHAAANIVPVIAANRYGLEevtpseeng 226 Streptococcus p...
ZP_03461426  148 IGVGICWDQWFPESARCMALDGAELLFYPTAIGSEPildc-dsmPHWRRVMQGSSAANIMPLIAANRIGTEtvkpseang 226 Bacteroides pec...
ZP_01078488  148 IGVAICWDQWFPESARSMVLKGAEMLFYPTAIGSEPsdanldsrEHWQQVMQGHAAANMVPVIASNRIGEEtts------ 221 Marinomonas sp....
XP_001692986 149 IGIAICWDQWFPEAARALALQGAEVVLFPTAIGSEPqdpaldsyGHWVRVQQGHAGSNLVPIIVSNRIGAErlppp---- 224 Chlamydomonas r...
Feature 1                                                                          
XP_001755668 227 rgpsKIAFYGTSFIAGPtGEIVASADnk-sEKVLVAKFDLNKIKVKRhsWGIFRDRRPELYKVLLT 291 Physcomitrella patens subsp. ...
YP_001825000 252 ----GLTFYGSSFIVDPyGRVLAQAPrd-ePAVLVADLDLDARRDWLelFPFLTTRRPDAYGPLTV 312 Streptomyces griseus subsp. g...
EED34229     248 ----TVEFYGSSFIVDPfGRLLAQAPrd-kEVTLVADIDLGWREDWLtlFPFLATRRPDTYAALCD 308 gamma proteobacterium NOR51-B
ACH68605     218 --giQVTFVGSSFITGQtGEMLKIADre-sEGVLVESFDLEKFHVRRasWGLIRDRRPNMYGALMT 280 Phytophthora sojae
YP_331750    222 --sgEIVFYGSSFIAGAdGEMIVECDrh-gEAIATAEFDLDALAYRRrgWGVFRDRRPECYRALSE 284 Burkholderia pseudomallei 1710b
XP_001690094 229 -stgPVTYYGGSFIAGPqGQILAQLA----EGLVTAEVDLAGLATARaqWGLFRDRRPDLYGALMT 289 Chlamydomonas reinhardtii
NP_345406    227 gqssSLDFYGSSFMTDEtGAILERAErq-eEAVLLATYDLDKGASERlnWGLFRDRRPEMYRQITD 291 Streptococcus pneumoniae TIGR4
ZP_03461426  227 gqssSLRFYGSSFITDEtGGVVESIDmrtkEGVIIHTFDLDAIREMRlsWGVFRDRRPEMYGAIMH 292 Bacteroides pectinophilus ATC...
ZP_01078488  222 --dsSMTFYGSSFIADYtGKKIEEADqt-sEAVICASFDLDEAQEYRrtWGIFRDRRPSQYTAINR 284 Marinomonas sp. MED121
XP_001692986 225 hqaaGNTFYGGSFIAGPqGQVLAQVRcs-aRGFCVAEVDLGAAAAARaaWGVYRDRRPELYGALAT 289 Chlamydomonas reinhardtii

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap