1EMS,1F89,1F89,2W1V


Conserved Protein Domain Family
nit

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cd07572: nit 
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Nit1, Nit 2, and related proteins, and the Nit1-like domain of NitFhit (class 10 nitrilases)
This subgroup includes mammalian Nit1 and Nit2, the Nit1-like domain of the invertebrate NitFhit, and various uncharacterized bacterial and archaeal Nit-like proteins. Nit1 and Nit2 are candidate tumor suppressor proteins. In NitFhit, the Nit1-like domain is encoded as a fusion protein with the non-homologous tumor suppressor, fragile histidine triad (Fhit). Mammalian Nit1 and Fhit may affect distinct signal pathways, and both may participate in DNA damage-induced apoptosis. Nit1 is a negative regulator in T cells. Overexpression of Nit2 in HeLa cells leads to a suppression of cell growth through cell cycle arrest in G2. These Nit proteins and the Nit1-like domain of NitFhit belong to a larger nitrilase superfamily comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes, which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13), this subgroup corresponds to class 10.
Statistics
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PSSM-Id: 143596
View PSSM: cd07572
Aligned: 214 rows
Threshold Bit Score: 299.342
Threshold Setting Gi: 93005053
Created: 21-Apr-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 10 residues -Click on image for an interactive view with Cn3D
Feature 1:putative active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                               #                                        
1EMS_B        16 FIAVCQMTsd-nDLEKNFQAAKNMIERAGEkKCEMVFLPECFDFIGlnkneqid---lamatdceYMEKYRELARKHNIW 91  Caenorhabditis ...
YP_001086961   5 KIAVCQMItt-eNKIENINHAVDMVTEAAInGAKIVVLPEMFNCPYenkyfpkf----aeeypgeTTTILSKLAEKHGIY 79  Clostridium dif...
YP_001273073   4 KIALCQINvv-dNKEKNIENATSMILKAVKqNADFIVLPEMFNCPYsnekfiey---ceeethspTLSKIAKLANENNTY 79  Methanobrevibac...
YP_002471916  19 KIGLCQMMvlksSKSDNVKRAKEAIYKAANkGTDIVALPEMFNCYYntkyfreya--ekddckgeTLSMLSSAAKEMGIY 96  Clostridium klu...
ZP_03783709   39 KVALLQTHvs-eKKEENLSVVREKLRALRAeKPDLVTLPEMFNCPYqtdqfpvy----aepqggeSWQALSCMAKEEGIY 113 Blautia hydroge...
ZP_01964326    5 KIAAIQMStv-aDKMENVRTVKTYLEKIKDeNPDFVILPEMFCCPYqtenfpiy----aekeggpVWQQLSGYAKQYGIY 79  Ruminococcus ob...
ZP_02996058    5 KIALCQMQvq-kEKKKNIEKAIEMLTKAKEeNCNIAILPEMFNCPYenkcfkpygeiineenggeTVKAIKKAANDLNLY 83  Clostridium spo...
YP_001716733   5 KLGICQMPvt-aSKEQNLSRARQMAAEAARaGARVVVLPEMFNCPYqhefftrf---aetcpdgdTFRMLTSTARELGVY 80  Candidatus Desu...
ZP_03759054    5 RAAMLQTRvf-aDKMENIREAGRKLEALEAeQVDLVTLPEMFNCPYqtpnfpvy----aepqggpAWTACADLARKHKIY 79  Clostridium asp...
YP_002506052   6 KLGLCQMAvt-dSKNENVKKAVFMLEECCKrGADIAVLPEMFNCPYdtklfply---aenfenskTLSVISDSAKYNNMY 81  Clostridium cel...
Feature 1                          #                #   #           #                         ## 
1EMS_B        92 LSlGGLHHKdpsdaaHPWNTHLIIDSDGVTrAEYNKLHLFDLEIPGkvrlMESEFSKAGTemiPPVDTpigRLGLSICYD 171 Caenorhabditis ...
YP_001086961  80 LVsGSIPELed---gKIYNTCYVFDKNGTLiGKHRKMHLFDIEVTGkvsfKESDTLTAGNd-vTVIDTeygKVGIAICYD 155 Clostridium dif...
YP_001273073  80 ILaGSIPEKeg---sKIFNTSYLFDKNGEIiAKHQKMHLFDIDVKGkiyfKESDTLSSGNk-vTIAKTdfgKVGIGICYD 155 Methanobrevibac...
YP_002471916  97 IVgGSIPEIdd--kgNIYNTSFVFNEKGELiGKHRKMHLFDIDIKDkitfKESNVLTPGDk-vTVIDTkwgKIGVAICYD 173 Clostridium klu...
ZP_03783709  114 LAaGSVPEVde--egKVYNTAYVFDRQGRQiAKHRKMHLFDINVTGgqyfKESDTLTAGDa-iTVFDTefgRMGICICFD 190 Blautia hydroge...
ZP_01964326   80 LIgGSMPEKda--egNVYNTSYIFDREGKQiGKHRKVHLFDIDVKGgqtfKESDMLTAGDs-dTVFDTefgKIGVMLCFD 156 Ruminococcus ob...
ZP_02996058   84 IVaGSIPEIeg---dKVYNTSMIVDNKGALiTKHRKIHLFDIDVKGgvtfKESDTLTAGNk-iTLFDTpwgKLGVMICYD 159 Clostridium spo...
YP_001716733  81 LIgGSIPEAed---gRTYNTCFVYGPDGRMlGRQRKLHLFNIETDDlv-fRESDTLSPGTgppTVFVTplvTFGVAICFD 156 Candidatus Desu...
ZP_03759054   80 LSaGSMPEVde--tgRVYNTAYVFDRQGRQiAKHRKAHLFDIDVKGgqcfKESDTLTAGDq-vTVFDTefgTMGLCICYD 156 Clostridium asp...
YP_002506052  82 IVaGTIPEFsn---gCIYNTSIMFDRQGKIiAKHRKIHLFDVNIKDgvsfRESDVLAAGRs-vTVAQTefgRIGLAICFD 157 Clostridium cel...
Feature 1        ##                    #                                                         
1EMS_B       172 VRFPELSLWNRkrgAQLLSFPSAFTlNTGLAHWETLLRARAIENQCYVVAAAQTGAHnpkrqsYGHSMVVDPWGAVVAQC 251 Caenorhabditis ...
YP_001086961 156 IRFPELSRLMAlkgAEIVILPAAFNmTTGPAHWELSIRMRALDNQIFYVGAAPARNMnasyiaFGNSRISDPWGRIIAQA 235 Clostridium dif...
YP_001273073 156 IRFPELAKLMAeagAQILFYPGAFNmTTGPAHWELTFRSRALDNQVYCVGVAPALNKdasyhsYGHSIIANPWGEVIVQL 235 Methanobrevibac...
YP_002471916 174 VRFPELMRLMAlqgAKIIFIPASFNtTTGPSHWELLFRSAAVENQLYTVGISPARNInysyvaYGNSLVVDPWGKILNIL 253 Clostridium klu...
ZP_03783709  191 IRFPELSRLMAqegARLILIPGAFNmTTGPAHWELSFRARALDNQVYMLGTAPARDEkssytsWGHSIVVNPWGEVQAQL 270 Blautia hydroge...
ZP_01964326  157 IRFPELSRMMVndgAKVIFVPAAFNmTTGPAHWELSFRTRALDNQIYMVGCAPARDVsagyisWGHSIVTDPWGRVTGML 236 Ruminococcus ob...
ZP_02996058  160 IRFPELSRIMAlkgAKIIFTPAAFNmTTGPAHWDTLFKSRALDNQIYMVGVAPARNEnsnyisYGNSLIASPWGNIVGRL 239 Clostridium spo...
YP_001716733 157 LRFPELFRDLAargAELIVAPAAFNtFTGPPHWELLLRARAVDNQVFVAGAGPAWTEdspypyYGHSLVVNPWAEVIACA 236 Candidatus Desu...
ZP_03759054  157 FRFPELARLMVekgAKVILVPAAFNmTTGPAHWEILFRCRALDNQAFVLGTAPARDPssgytsWGHSIAVSPWGDILGQM 236 Clostridium asp...
YP_002506052 158 MRFTELYSQMSeagAKIIITPASFNmTTGPVHWELLVRARALDNQIFHAAVSSARNTsdtyisYGNSMVCDPWGRVISKA 237 Clostridium cel...
Feature 1                                      
1EMS_B       252 SErvDMCFAEIDLSYVDTLREMQPVFSHRR 281 Caenorhabditis elegans
YP_001086961 236 DEkeCIIYADIDRDLIPDIRQQLPLLKHRR 265 Clostridium difficile 630
YP_001273073 236 DEqeNMEIAEIDLEEIKKVRTELPLLKNKR 265 Methanobrevibacter smithii ATCC 35061
YP_002471916 254 DEkeGILLSEIDLEYIYEVRKSIPVFEHRR 283 Clostridium kluyveri NBRC 12016
ZP_03783709  271 DEkeGCLVQELNLDEIDRIRRDLPLLSSRR 300 Blautia hydrogenotrophica DSM 10507
ZP_01964326  237 DEneGILLAELDMDYEEQVREELPLLKSRR 266 Ruminococcus obeum ATCC 29174
ZP_02996058  240 GAeeNILFSEIDLDYENKIREELPLLRHIR 269 Clostridium sporogenes ATCC 15579
YP_001716733 237 GEreTVLLAEIDLGQVAEVRKKLPLHRTEK 266 Candidatus Desulforudis audaxviator MP104C
ZP_03759054  237 DEreGCMIHTLDLERAGEVRNQLPLLKHRR 266 Clostridium asparagiforme DSM 15981
YP_002506052 238 DEkeGILIADIDLNMVNSVRSQIPVNKIID 267 Clostridium cellulolyticum H10

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