3DLA,3DLA,3DLA


Conserved Protein Domain Family
GAT_Gln-NAD-synth

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cd07570: GAT_Gln-NAD-synth 
Click on image for an interactive view with Cn3D
Glutamine aminotransferase (GAT, glutaminase) domain of glutamine-dependent NAD synthetases (class 7 and 8 nitrilases)
Glutamine-dependent NAD synthetases are bifunctional enzymes, which have an N-terminal GAT domain and a C-terminal NAD+ synthetase domain. The GAT domain is a glutaminase (EC 3.5.1.2) which hydrolyses L-glutamine to L-glutamate and ammonia. The ammonia is used by the NAD+ synthetase domain in the ATP-dependent amidation of nicotinic acid adenine dinucleotide. Glutamine aminotransferases are categorized depending on their active site residues into different unrelated classes. This class of GAT domain belongs to a larger nitrilase superfamily comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes, which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13), this subgroup corresponds to classes 7 and 8. Members of this superfamily generally form homomeric complexes, the basic building block of which is a homodimer. Mycobacterium tuberculosis glutamine-dependent NAD+ synthetase forms a homooctamer.
Statistics
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PSSM-Id: 143594
View PSSM: cd07570
Aligned: 296 rows
Threshold Bit Score: 155.32
Threshold Setting Gi: 227486404
Created: 21-Apr-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 10 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:glutaminase active site
  • Structure:3DLA_C; Mycobacterium tuberculosis glutamine-dependent NAD+ synthetase monomer bound with DON inhibitor in the GAT domain; contacts calculated at 4A
    View structure with Cn3D
  • Comment:This mimics the gamma-glutamyl thioester intermediate.
  • Comment:GAT_Gln-NAD-synth is a bifunctional enzyme having a NAD+ synthetase domain in addition to this GAT domain.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                               #                                        
3DLA_C        14 RVAACTHHttIGDPAANAASVLDMARACHddgAALAVFPELTLSGYSIEDVLLQDSLLDAVEDALLDLVTESad-lLPVL 92  Mycobacterium t...
ZP_03898456    5 KVASGVVNqtPMAWEANTRNITEAIREARkqqVSLLCLPELCISGYGCDDYFFAPDMVEQAQRCFLEIVDETa---GMIV 81  Dyadobacter fer...
NP_501866      9 RVATCTVNnwALDFKGNYERIVKTCEEAAalgARIRLGPELEIPGYGCADHFFELDTERHSWEMLSKLVEKSkkwpNLLV 88  nematode
ZP_03128809    5 HVAAATLNqtPLDWEGNKRNILAAIAEARvrgVSVLCLPELCITGYGCEDAFHSAATHATAWEVLQEILPATr---GMIV 81  Chthoniobacter ...
ZP_01694259    5 KVAAAILNqtPLDWEQNTQNIIEAINNARnqgVSLLCLPELCITGYGCEDAFYAPNTCAQALEVLVKILPHTq---GMVV 81  Microscilla mar...
ZP_01092096    5 KVAAAVVNqtPLDWQGNCDRILAAIEEANlneASILCLPELCITGYGCEDAFLSADVQRTALAMLHMIAPRTr---DMFV 81  Blastopirellula...
ZP_01853804    5 QVAAVALNqtPLDWAGNVKNIKTAISTARdqgASLISLPELCITGYGCEDAFFSPDVQQRALHALQELLPLTe---GIVV 81  Planctomyces ma...
YP_002434090   5 RIAAASLNqtPLDWKGNMARIRQAVSLAEeagANFLLLPELCITGYGCEDAFSAHFVIDCSHRFLVALARETp---NMAV 81  Desulfatibacill...
ZP_04494093    5 KVAAGVLNqiPLAWEHNKQNIINAIEEAQrqnVSLLCLPEFCISGYGCEDAFFAQNTIDQSIASLLEIVEHTn---DIVV 81  Spirosoma lingu...
NP_713170      5 RLTSVSLKtkVFDFQGNLEKIKRVLKLEKk--SDLILFPELCISGYGCEDAFYFPRIWKRSWNSLIQLLPHTe---NKII 79  Leptospira inte...
Feature 1                                     #   # #  # #                                       
3DLA_C        93 VVGAPLRhrhrIYNTAVVIHRGAvLGVVPKSYLPTYREFYERRQMAPGDgergtirig--------gadvafgtdLLFAA 164 Mycobacterium t...
ZP_03898456   82 AVGLPLRhngsLYNAACLISNKQiAGFYCKQNLANNGIHYEARWFKPWQpgvvesievnqmfypigdvifdvaagPILGG 161 Dyadobacter fer...
NP_501866     89 VTGLPTRfrglLYNCAAALRNGKlLFIRAKMGLADDNVYRESRWFVKWTetfkhyqmplnsdihfdqetvpfgdgILESS 168 nematode
ZP_03128809   82 SLGLPIFhrnaLFNTAGLVVDGRiVGFVGKQFLAGDGIHYEPRWFKPWPsglqstiern-------grscpigdlCFDCG 154 Chthoniobacter ...
ZP_01694259   82 SVGLPLFvqnqLFNTACLIVNGKvAGFVAKKFLAGQGIHYEQRWFKAWKsgeittiqlpem---lggaevkvgdvYFDIG 158 Microscilla mar...
ZP_01092096   82 TLGLPMSyrgvLYNVVAVLADGEiVGFVPKQNLAGDGIHYEPRWFKPWPeglraevele-------grtypfgdlVFRID 154 Blastopirellula...
ZP_01853804   82 SVGLPLFyggaLYNCACLISNQRiLGFVAKNHLAGDGIHYEPRWFKAWNsphvsqveie-------gqsyplgnlVFDCG 154 Planctomyces ma...
YP_002434090  82 IVGLPVLhrkaVYNCAAVLAGGKvLGLVPKQHLAGDGLHYEPRWFRPWKpgvqdewq----------ggvpmgdiDFDVN 151 Desulfatibacill...
ZP_04494093   82 AVGLPLRhnnrTFDTACLIANKRiLGFTAKQYMANNGVHYETRWFQPWPpyirdeikig-------dftypfgdvVYDLS 154 Spirosoma lingu...
NP_713170     80 VVGLPIFqnpyLFNCAAVLCNGVvAGIVPKSNLASTGVHYENRWFTRGEesqenfiapd-------gstipfgslIFETD 152 Leptospira inte...
Feature 1                    ##  #                            #                                  
3DLA_C       165 sdlpgfVLHVEICEDMFVPMppSAEAAlaGATVLANLSGSPITIGRAEDRRLLARSASARC-LAAYVYAAagegESTTDL 243 Mycobacterium t...
ZP_03898456  162 sh--gvKIAFEICEDGWVANrpARRHYerGVDIILNPSASHFAFNKFMVREKLVVDASRSF-SCSYIYTNll-gNEAGRA 237 Dyadobacter fer...
NP_501866    169 dn---vRIGFEICEELWSARstNVRLAeqGVDIMCNGSGSHHILGKSNYRINQLILGSSAKvGGVYLYANqr-gCDGDRV 244 nematode
ZP_03128809  155 d----iRIGFEICEDAWVAArpGANLArqSVDLLLNPSASHFAFGKIEVRRRFVAEGSRAF-GLTYVYANmv-gNESGRA 228 Chthoniobacter ...
ZP_01694259  159 g----vKIGYEICEDAWVANrpGRDLYkyGIDILLNPSASHFAFGKLEIRKRFVLEGSRAF-GVSYIYANll-gNEAGRA 232 Microscilla mar...
ZP_01092096  155 d----aLIGLEICEDAWVADrpGSRQAriGVDIILNPSASHFAFGKHEIRQRFVLEGSRAF-HTSYVYANll-gNEAGRA 228 Blastopirellula...
ZP_01853804  155 g----iRIGFEICEDAWAARrpGRDLSqaAVDLILNPSASHFAFGKQDIRRRLVLESSRAY-GVSYIYSNll-gNEAGRI 228 Planctomyces ma...
YP_002434090 152 g----iRFGLEICEDAWVADrpGARLArrGADIIFNPSASHFSIGKTRIRRNFVIDGSRAF-GCAYVYANll-gNEAGRA 225 Desulfatibacill...
ZP_04494093  155 g----iRIGFEICEDAWVASrpGRLLYerGIDIILNPTASHFAFLKSQVRERFVVDASRSF-GVSYVYSNml-gNEAGRI 228 Spirosoma lingu...
NP_713170    153 h----fSFGVEICEDSWVLQkpSIPLAeaGTDLILSPGASHFAFGKQRIRRQIFQESSRRE-SNVYLFSNlc-gNESGRL 226 Leptospira inte...
Feature 1                                                                                  
3DLA_C       244 AWDGQTMIWENGALLAESERfpk-gvRRSVADVDTELLRSERlrm------------------gtfDDNRRHHR 298 Mycobacterium tubercu...
ZP_03898456  238 VYDGDAMIASNGDLLASSPRfsyedfLITTAVIDTEYTRLSQiqs------------------kitVPPKERTW 293 Dyadobacter fermentan...
NP_501866    245 YYDGASSVAQNGDLLAQIHQfdiedtSVVSAVVDLSDNQCFRhmk------------------ssdRGNASDQV 300 nematode
ZP_03128809  229 IFDGGALIATGGRLVAEGNRfsfadcEVTSAVVDLERTRMGQart------------------gsfQPDLQSSE 284 Chthoniobacter flavus...
ZP_01694259  233 IYDGGALVATNGEMIATGKRfsyanwEVTTTTIDIELTRLAQiqnqipf-----------dtaddyRHRVQCDF 295 Microscilla marina AT...
ZP_01092096  229 IYDGDAMIASGGRMLAIGSRlsfhayLVTTAVIDLDLTRMYRarsda--------------frpdyQGSLQPVV 288 Blastopirellula marin...
ZP_01853804  229 IYDGATLIASNGTLLAEGPRlsfkavVLTTAVIDIDLTRMNRarlmsfqpdqlgktenrvvapfqfPKIEPQPT 302 Planctomyces maris DS...
YP_002434090 226 VYDGGNMIAFAGELTAASPRlgfedvVLTTATVDVDLGRAKQartgsfepm------iepdgdcikAAIEWEDV 293 Desulfatibacillum alk...
ZP_04494093  229 IYDGDAMVASNGELLVSGPRlsyedfVIVPAVIDVEATRLNQtq---------------------nRANIALAY 281 Spirosoma linguale DS...
NP_713170    227 IFEGGSMIVQNGKLLSESERlffgdfSLCSNEIDFEASQADRakn------------------frpSGNRFSKT 282 Leptospira interrogan...

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