2VHI,2VHI


Conserved Protein Domain Family
ML_beta-AS_like

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cd07568: ML_beta-AS_like 
Click on image for an interactive view with Cn3D
mammalian-like beta-alanine synthase (beta-AS) and similar proteins (class 5 nitrilases)
This family includes mammalian-like beta-AS (EC 3.5.1.6, also known as beta-ureidopropionase or N-carbamoyl-beta-alanine amidohydrolase). This enzyme catalyzes the third and final step in the catabolic pyrimidine catabolic pathway responsible for the degradation of uracil and thymine, the hydrolysis of N-carbamyl-beta-alanine and N-carbamyl-beta-aminoisobutyrate to the beta-amino acids, beta-alanine and beta-aminoisobutyrate respectively. This family belongs to a larger nitrilase superfamily comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes, which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13), this subgroup corresponds to class 5. Members of this superfamily generally form homomeric complexes, the basic building block of which is a homodimer. Beta-ASs from this subgroup are found in various oligomeric states, dimer (human), hexamer (calf liver), decamer (Arabidopsis and Zea mays), and in the case of Drosophila melanogaster beta-AS, as a homooctamer assembled as a left-handed helical turn, with the possibility of higher order oligomers formed by adding dimers at either end. Rat beta-AS changes its oligomeric state (hexamer, trimer, dodecamer) in response to allosteric effectors. Eukaryotic Saccharomyces kluyveri beta-AS belongs to a different superfamily.
Statistics
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PSSM-Id: 143592
View PSSM: cd07568
Aligned: 6 rows
Threshold Bit Score: 505.108
Threshold Setting Gi: 29828492
Created: 21-Apr-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 9 residues -Click on image for an interactive view with Cn3D
Feature 1:putative active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                          #                            
2VHI_C       70 RRIVRVGAIQNSiviptt-apiekqREAIWNKVKTMIKAAAeAGCNIVCTQEAWTMPfAFCTREKfPWCEFAEEaenGPT 148 fruit fly
2VHI_D       70 RRIVRVGAIQNSiviptt-apiekqREAIWNKVKTMIKAAAeAGCNIVCTQEAWTMPfAFCTREKfPWCEFAEEaenGPT 148 fruit fly
ZP_04357313   2 SRIIKSGLIQMSlpktegegtieeiKEAMIQKHIPLIEEAGeKGVQILCLQEIFDTP-YFCPGQDaKWYASAETv-pGPT 79  Chitinophaga pin...
YP_828847     2 SRIVRCSLIQATnaappd-asldatKKAMLDKHVAYIEQAAkAGAQIVCLQEIFYGP-YFCAEQTtKWYDFTEPipdGPT 79  Solibacter usita...
AAO66293      2 ADKVTIGLIQAKndvhgd-epvhlhKEKAIEKHVKMVREAAgKGAQIICLQEIFYGP-YFCAEQStKWYEAAEEvpnGPT 79  Brevibacillus agri
NP_823126     2 SRVIRAAVFQTAwt---------gdKESMIQVHEQAVRDAAaQGAQVLCFQELFYGP-YFCQVQDkAFYEYAEQiphGPI 71  Streptomyces ave...
Feature 1                                                       #   #            #              
2VHI_C      149 TKMLAELAKAYNMVIIHSILERDmehgETIWNTAVVISNSGRYLGKHRKNHIPRVGD------FNESTYYMEGNTGHPVF 222 fruit fly
2VHI_D      149 TKMLAELAKAYNMVIIHSILERDmehgETIWNTAVVISNSGRYLGKHRKNHIPRVGD------FNESTYYMEGNTGHPVF 222 fruit fly
ZP_04357313  80 TERMAAYAKKYNMVIIVPIYEKEq--aGVLYNTAAVIDADGTYLGKYRKNHIPHTSG------FWEKFFFKPGNLGYPVF 151 Chitinophaga pin...
YP_828847    80 IKLMQDLARRHHVVLIVPIYEIEq--eGIYYNTAAVIHNDGTYLGKYRKTHIPHVAPg-----FWEKFYFRPGNLGYPIF 152 Solibacter usita...
AAO66293     80 VQQFSALGKELGTVLILPVYEKVg--iGTYYNTAAVIDADGTYLGKYRKQHIPHVGVgssgcgFWEKYYFKPGNLGYPVF 157 Brevibacillus agri
NP_823126    72 VKRFQALARELGIVLVLPMYEEEq--pGVLYNTAAVIDADGSYLGKYRKTHIPQVEG------FWEKFYFRPGNSGWPVF 143 Streptomyces ave...
Feature 1                  ## ##                    #                                           
2VHI_C      223 ETeFGKLAVNICYGRHHPQNWMMFGLNGAEIVFNPSATIGrLSEPLWSIEARNAAIANSYFTVPINRVGTeqfpneytsg 302 fruit fly
2VHI_D      223 ETeFGKLAVNICYGRHHPQNWMMFGLNGAEIVFNPSATIGrLSEPLWSIEARNAAIANSYFTVPINRVGTeqfpneytsg 302 fruit fly
ZP_04357313 152 QTrYAKVGVYICYDRHFPDGARILGLNGAEIVYNPSATVAgLSQYLWKLEQPAHAAANGYFMGCINRVGEekpw------ 225 Chitinophaga pin...
YP_828847   153 DLgFVKIGVYICYDRHFPEGARALGLAGAEIVFNPSATVAgLSEHLWKLEQPAHAVANGYFVGAINRVGTeapw------ 226 Solibacter usita...
AAO66293    158 DTaFAKVGVYICYDRHFPEGARLLGLGGAEIVFNPSATVAgLSEYLWKLEQPAHAVANGYYVAAINRVGTeapw------ 231 Brevibacillus agri
NP_823126   144 DTaVGRIGVYICYDRHFPEGWRALGLAGAEIVFNPSATSRgLSGYLWQLEQPAAAVANEYFVGAINRVGVeelg------ 217 Streptomyces ave...
Feature 1                                                                              
2VHI_C      303 dgnkahkefgpFYGSSYVAAPDGSRTPSLSRd-KDGLLVVELDLNLCRQVKDFWGFRMTQRVPLYAESFKK 372 fruit fly
2VHI_D      303 dgnkahkefgpFYGSSYVAAPDGSRTPSLSRd-KDGLLVVELDLNLCRQVKDFWGFRMTQRVPLYAESFKK 372 fruit fly
ZP_04357313 226 -------nigkFYGSSYFVDPRGQIFASASEd-KDELLIASFDLDMIDEVRNVWQFFRDRRPETYGKLTEL 288 Chitinophaga pinensis DSM...
YP_828847   227 -------nigeFYGQSYFCDPRGRIFAQASRd-KDEVLTADLDLDLIAEVRKTWQFFRDRRPDMYESLVKA 289 Solibacter usitatus Ellin...
AAO66293    232 -------nmgeFYGQSYLVDPRGQFVAVGSRd-QDEVIVAEMDREKIREVRDTWQFYRDRRPETYDDMVKL 294 Brevibacillus agri
NP_823126   218 --------dndFYGTSYFVDPEAQFVGEVASdkETELVVRDLDLAKLREVRDRWQFYRDRAPGAYGPLTAP 280 Streptomyces avermitilis ...

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