Conserved Protein Domain Family
ScNTA1_like

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cd07566: ScNTA1_like 
Saccharomyces cerevisiae N-terminal amidase NTA1, and related proteins (class 3 nitrilases)
Saccharomyces cerevisiae NTA1 functions in the N-end rule protein degradation pathway. It specifically deaminates the N-terminal asparagine and glutamine residues of substrates of this pathway, to aspartate and glutamate respectively, these latter are the destabilizing residues. This subgroup belongs to a larger nitrilase superfamily comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes, which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13), this subgroup corresponds to class 3.
Statistics
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PSSM-Id: 143590
View PSSM: cd07566
Aligned: 19 rows
Threshold Bit Score: 354.337
Threshold Setting Gi: 116057596
Created: 21-Apr-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
Feature 1:putative active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                       #                                
P40354        20 KVLVIQLNPQIGQVDQTIKRTWSILDKVtksa----tyvkPDIILFPEFALTGYsFHARKDILPYVtkkdegpsFELAKS 95   baker's yeast
XP_360222      2 RIACLQFAPQVGDVDNNLNRADAIISKThpsq-----lehLDLLVLPELAFTGCnFKSLNEISPFLelsgfgisSLWART 76   Magnaporthe gr...
XP_001585699   2 RIACLQFAPQVGDVDNNFKKADAILSKAdp--------edIDLLVLPEMAFSGYnFTSLGHITPYLepstsgvtSAWART 73   Sclerotinia sc...
XP_001931433   2 KVACLQFAPEVGQVQENIQRADDILSRTrip-------ddLDWLVLPEMAFSGYnFHTLDEIKPYLeptasgvsTKWAQQ 74   Pyrenophora tr...
XP_001229151   2 KIACLQFNSQVGEVDDNLNRADAVLDRIdped-----ldgLDLLVLPELAFSGYnFKSLQHITPCLeeagsgitSLWAQT 76   Chaetomium glo...
EDK39597       4 RIAAVQTTAVVGQVKSNMANISKLVASSiar-------tpVDILLLPELALTGYnFKSPGAIEPFLeptaagtsTEFARS 76   Pichia guillie...
XP_503133     14 QIGCLQLKPEIGEVDKNMGVANELIDRYyatakkddktpkIDILMLPEMSFTGYnFESPENLKPFReetessksIEWARE 93   Yarrowia lipol...
XP_961380      2 RIGCLQFAPQVGDVSNNLSRADAVLAKAnqre-----ledLDLLVLPEMAFSEEiQPFLEPSGSGIs-------ALWART 69   Neurospora cra...
EEQ37205       4 RVACVQFNPLLGQVEANITRVKTLLSSVqk---------eIDLIVLPELSLSGYnFSSPQEIKPFLedstqgksCSLARE 74   Clavispora lus...
EER27389       2 RIATLQFSPRLRDVEGNIERGEEILNKWeqshg--dearkLDLLVLPEMAFTGYnFSSLETIKPYLeptrsgptAKWARS 79   Coccidioides p...
Feature 1                                                                    #   #  #            
P40354        96 ISEKFQCYTIIGYPEDdde--------------------qklYNSALVVNPQGeqiFNYRKTFLYdTEMNWDCEENPEGF 155  baker's yeast
XP_360222     77 TALKHNCHVVVGYPEKvdvepiw-------------ptspeyYNSCIMVDGEGetvGNYRKSFLD-VDERWALEGRDGFF 142  Magnaporthe gr...
XP_001585699  74 IALKHNCIVTVGYPEKvdhfsrv-------------sarpeyYNSAVTVNKEGepiVNYRKSFLYyTDETWAHEGSGFFS 140  Sclerotinia sc...
XP_001931433  75 VAKHYNCHVTIGYPEItdpstnp-------------tdtqtqYNSTVTVSPQGevlHNYRKSFLYyTDETWASEGPGSRS 141  Pyrenophora tr...
XP_001229151  77 TALKHDCTVVVGYPEKvdvsanw-------------paspeyYNSALAINGDGdsvGNYRKSFLFyTDETWALEGGNEFF 143  Chaetomium glo...
EDK39597      77 LSKKYNCFTLIGYPEKeks---------------------itYNAAVFTSPAGdvlHHYRKCHLYeTDEVWGCSENPGPK 135  Pichia guillie...
XP_503133     94 CAKKIDGHVLVGYPEFspqd-------------------datYNSVSMVSPKGdilFTYRKSFLYtADEAMGMSESPDGF 154  Yarrowia lipol...
XP_961380     70 NALKYDCTVAVGYPEKvgssgsp------------rgsspeyYNSLLVVNGEGetvANYRKSFLYyTDQTWALEGGGFYG 137  Neurospora cra...
EEQ37205      75 LCKTYGCTTVIGYPEThqg---------------------ttYNSALVVDEEGnvvYNYRKTHLYeTDEAWGCKENPDKS 133  Clavispora lus...
EER27389      80 TARRLGCVVCVGYPEIratksdqtsvqesseaetsnlehekrFNSLIIEDSSGntlLNYQKCFLY-YTDEPWASEGENGK 158  Coccidioides p...
Feature 1                                         ## ##                         #                
P40354       156 qtfpmdfsk--caklsnedSYNRDVtlKASIGICMDLSPYKFMAPFNHFEFSsfcVDNNVELILCpMAWLNSTSITDKQT 233  baker's yeast
XP_360222    143 -------------------DEEVDGlgVIAMGICSDINPYKMEAPWASFEFSyhaLDSEARLVIVtMSWPTQEDIGQYSC 203  Magnaporthe gr...
XP_001585699 141 -------------------GEIDGLg-KVAMGICMDLNPYKFEAPWSTCEFAshvLEKKANLVIVsMAWLTREDQISFGL 200  Sclerotinia sc...
XP_001931433 142 hklanna-----spdnpffTGQLGAlgKVTLGICMDINPYKFVSPWSDYEFCslaLSSQSSLICIsMAWLCHLTPTELAK 216  Pyrenophora tr...
XP_001229151 144 k------------------GEIAGLg-NVALGIGTDLNPYKLEAPWDAFEFGfhvAKTQANVVIVsMAWQTHQDPDVFGA 204  Chaetomium glo...
EDK39597     136 fesfkfvad-keyyhgqkdPHEKLPtiTASTGICMDLNPYKFEAPFTAFEMArscLANGVSLILCpMAWLSITAVGGTNR 214  Pichia guillie...
XP_503133    155 qtyg------------pfpSLSQDIplKFQVGICMDINPYKFESAPSEYEFGnsvKKNKVPLVLLpMAWTHSKSPSLVPV 222  Yarrowia lipol...
XP_961380    138 -------------------GRMEGFg-NVAMGICMDINPYKFEAPWHKFEFAfhvLEVRANLVILsMAWLTHEDPSTFTP 197  Neurospora cra...
EEQ37205     134 fpsip----------ltlgKDSSRLtlRTNIGICMDLNPYKFEAPFNKFEFSissWLNDSSLIIVpTAWLSSDSPSIQES 203  Clavispora lus...
EER27389     159 gyfplpvsirskpgetlsnRSQSTVhiPTAVGICMDINPYKFIAPYTAYEFAthvLDSGAKLVIVsMAWLTWLTSEELAG 238  Coccidioides p...
Feature 1                                                                                        
P40354       234 LHNN--SLLEAAKNKIafalke------------------------------qglplaGSQGIYQLk-iGDSQRTPRvps 280  baker's yeast
XP_360222    204 QPSE--PDMAMVVYWTerlepli-----------------------------raendeETIVVFCNr-tGTEGDMTYags 251  Magnaporthe gr...
XP_001585699 201 LAKE--PDMKTISYWItrlepii-----------------------------raetdeEIIIVLANr-cGTEGEATYagt 248  Sclerotinia sc...
XP_001931433 217 DPSQ--PDVATVAYWVerfqplv-----------------------------eaksdrPFYVVLANr-cGMEKGLCYags 264  Pyrenophora tr...
XP_001229151 205 NPAE--PDIETLVYWVqrlepli-----------------------------radkeeEVIVVFCNr-aGSETEATYtgt 252  Chaetomium glo...
EDK39597     215 HPNQ--PETDTVGYWIqrfspl-------------------------------vesehPVAVVTCNr-vGQEDNLLFags 260  Pichia guillie...
XP_503133    223 KSSDkiPMTEEQEKTHse--------------------------------------lrNAIVHKCTmlhGKSTFDGLite 264  Yarrowia lipol...
XP_961380    198 LAQE--PDLATMTYWLqrfepvi-----------------------------rdesdeELIVVFCNr-cGMEDDVLYagt 245  Neurospora cra...
EEQ37205     204 WTRD--EKDKKASEWQkrftensideanveskslvdywilrffpflshpanglsqkphKTTVILCNr-tGIEGDVLYggs 280  Clavispora lus...
EER27389     239 EPQT--PDTDTFQYWIqrfwplit---------------------------rdswdgeEIIIVFANr-tGEEEGMEGk-- 286  Coccidioides p...
Feature 1                                                           
P40354       281 ------ddsTSEYKDMDEPDMSNVNYWILr----------fFPFLYFKSRI 315  baker's yeast
XP_360222    252 ------savLGIKDGEISIYGILGRGVKEl----------lVVDTDDPPLA 286  Magnaporthe grisea 70-15
XP_001585699 249 ------savLGIRDGEVSVYGILGRGEEEl----------lVVDTDEIPRA 283  Sclerotinia sclerotiorum 1980
XP_001931433 265 ------stvIRIEKGVVSLYETAGKSEETc----------lVVDLDERPKF 299  Pyrenophora tritici-repentis Pt-1C-BFP
XP_001229151 253 ------savIGIKRGEVFVYGVLGRGVDDll--------ivDTEQPPMSKL 289  Chaetomium globosum CBS 148.51
EDK39597     261 s----aaftFEGKRNPTNPVKIAGALPCDq----------pAVRVFEMEVA 297  Pichia guilliermondii ATCC 6260
XP_503133    265 pk---eipkYKNEDLATFHDYTADVDNKLpl---------vDTLQYWIERL 303  Yarrowia lipolytica CLIB122
XP_961380    246 ------savIGIKNGEVSVYGLLGRGSKEl----------lVVNTDAPPYG 280  Neurospora crassa OR74A
EEQ37205     281 ssilqfdplKGSSDDLDSANPSVEVVGSAg----------wANDQVLYREI 321  Clavispora lusitaniae ATCC 42720
EER27389     287 --------dTARYAGTSCVIGIRKANADDgdnskeeerryfDVDIVVWERL 329  Coccidioides posadasii C735 delta SOWgp

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