1R6V


Conserved Protein Domain Family
Peptidases_S8_Fervidolysin_like

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cd07485: Peptidases_S8_Fervidolysin_like 
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Peptidase S8 family domain in Fervidolysin
Fervidolysin found in Fervidobacterium pennivorans is an extracellular subtilisin-like keratinase. It is contains a signal peptide, a propeptide, and a catalytic region. The tertiary structure of fervidolysin is similar to that of subtilisin. It contains a Asp/His/Ser catalytic triad and is a member of the peptidase S8 (subtilisin and kexin) family. The catalytic triad is similar to that found in trypsin-like proteases, but it does not share their three-dimensional structure and are not homologous to trypsin. Serine acts as a nucleophile, aspartate as an electrophile, and histidine as a base. The S53 family contains a catalytic triad Glu/Asp/Ser with an additional acidic residue Asp in the oxyanion hole, similar to that of subtilisin. The serine residue here is the nucleophilic equivalent of the serine residue in the S8 family, while glutamic acid has the same role here as the histidine base. However, the aspartic acid residue that acts as an electrophile is quite different. In S53, it follows glutamic acid, while in S8 it precedes histidine. The stability of these enzymes may be enhanced by calcium; some members have been shown to bind up to 4 ions via binding sites with different affinity. There is a great diversity in the characteristics of their members: some contain disulfide bonds, some are intracellular while others are extracellular, some function at extreme temperatures, and others at high or low pH values.
Statistics
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PSSM-Id: 173811
View PSSM: cd07485
Aligned: 8 rows
Threshold Bit Score: 320.586
Threshold Setting Gi: 56553650
Created: 1-Jun-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
active sitecatalytic triad
Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                 #                                       
1R6V_A        146 QLWEEasg------------------------tnIIVAVVDTGVDGTHPDLegq---------------viAGYRpafde 186  Fervidobacter...
YP_001471655  170 EAWQMgyt-----------------------gegVIVAVVDTGVDGTHPDLqgq---------------lvEGYRpltge 211  Thermotoga le...
YP_001567802  181 AAWATatg------------------------agVIVGLLDTGTDGTHPDLvnq---------------lkEGYDpywnk 221  Petrotoga mob...
ZP_03300901   172 KQWYFdnpgteswqregadirlidvwkqyngnptIIVAVVDGGINQDHPDLqdnlwtnpgeipengidddgNGYIddvhg 251  Bacteroides d...
NP_809125     220 EAWKKctg-----------------------dpsIIVAVLDEGVMYTHPDLkgnmwinekeelyadkdadgNGYKddkyg 276  Bacteroides t...
YP_446403     176 DQYGFqnie---------------------aeggWSTRTGDTTVVVSIHDSgit--------------gnfDGNEttvnh 220  Salinibacter ...
ZP_02424566   229 PAWEFctg-----------------------dpsIIVAVMDEGVMYKHEDLaanmwvnqae-lngqkgvddDGNGyvddv 284  Alistipes put...
Q9RYM8        171 AAWDAgft-----------------------gkgVKVGVIDDPSDVSHPDLrpn--------------wagKAYDpatnt 213  Deinococcus r...
Feature 1                                             #                                           
1R6V_A        187 elpa-----------------------gtdssyggSAGTHVAGTIAAKKDgkgivg------------vapgaKIMPIVi 231  Fervidobacter...
YP_001471655  212 tlpa-----------------------asdssfggAHGTHVAGTIAASDNsigvig------------vapnaKIMPIVi 256  Thermotoga le...
YP_001567802  222 kid------------------------psensdtdGHGTHTAGIIAAKDDgqgvvg------------lapdaKIMPIRi 265  Petrotoga mob...
ZP_03300901   252 ynfv----------------------ddnailvphRHGTHVAGTIGATNNngtgissiag----gngtpgsgvKLMSCQi 305  Bacteroides d...
NP_809125     277 ynfvsns-----------------giiswmdavdtGHGTHVAGTIAAMNNngegvcgiag----gdgsknsgvKIMSCQv 335  Bacteroides t...
YP_446403     221 pdlqpnvwyggdpsdgsvhgknfagdpanlvdntnGHGTHVTGTVAAVTNnafgvagtaggngefangrgtgiRYMVIP- 299  Salinibacter ...
ZP_02424566   285 ygynfakd---------------qgditwtdpedsGHGTHVAGTISAVNNngigvcgia-----ggsgnndgvKIMSIQi 344  Alistipes put...
Q9RYM8        214 tyttvqgwida--------idgfdgkvdnkvdpgiEHGTAVASTIAAAKNgqgivg------------vapdsKFYTAAi 273  Deinococcus r...
Feature 1                                                 ##                                      
1R6V_A        232 fddpalv--ggngyvgDDYVAAGIIWATdh--gaKVMNHSWGgwg---------------------ysYTMKEAFDYAMe 286  Fervidobacter...
YP_001471655  257 fdtgspetggdgsyigDDYVAQGFMWAFen--gaMIFSNSWGgkg---------------------ysITLAHTIAQIMs 313  Thermotoga le...
YP_001567802  266 fnp---------nyvgDYAVAAGIVWAVen--gaDILSNSWGggg---------------------ysNILKDAFDYALt 313  Petrotoga mob...
ZP_03300901   306 lkslts---tggevasDPFIAAAIKYGAdn--gaVISQNSWGyavgtgr------------ntssyinPVHKEAIDYFIk 368  Bacteroides d...
NP_809125     336 fage--------agvtLDAEARAIKYAAdn--gaVILQCSWGynsslanliegytpgpgseeeweklyPLEKDALDYFIn 405  Bacteroides t...
YP_446403     300 ----------------NLGEPDAYVYAAdn--gaVVSQNSWGytsp------------------gifpQSLQDAIDYFId 343  Salinibacter ...
ZP_02424566   345 fagr--------yqstISRNVDAIYYATsm--gaSILQCSWGlmsgains----------deqylderSIEANAFAHFIn 404  Alistipes put...
Q9RYM8        274 fq-----------pgfIGDYLVARSVIWtvnqgaQVINNSWGgtg---------------------ysPLLKQAFDYALe 321  Deinococcus r...
Feature 1                                                                                         
1R6V_A        287 h----------------gVVMVVSAGNNtsdshhq--ypagypgVIQVAALdyyggtfrvagfssrsDGVSVGAPGv--- 345  Fervidobacter...
YP_001471655  314 y----------------gGIFVASAGNShtdeihy---pscypgVINVAAStaqdgit---nfstrgRWVTVAAPGd--- 368  Thermotoga le...
YP_001567802  314 h----------------nVVVVASSGNFttdqhwf--ypsgfpgVIAVAASddkdnia---yfssrgDYVSVSAPGv--- 369  Petrotoga mob...
ZP_03300901   369 yagcdnngeqldgspmkgGIVLFASGNAntsdpria-apadydkVVAVAAIeadyrka---sysnygTYMDISAPGgaln 444  Bacteroides d...
NP_809125     406 nagsp-------ngvidgGLAIFASGNEyagmaafpaayskcisVSAVAADftpas------ysnygKEVTISAPGgdte 472  Bacteroides t...
YP_446403     344 nggg---------dhldgGIVVFSAGNDsdngdwf---paaydpIVSVASTdqndnvs---sfsnygEWVDIAAPGt--- 405  Salinibacter ...
ZP_02424566   405 tkrpg--------splngGIIIFAAGNEagacgy----paaypsVVCVTSLstdftps---vftnygMPADIAAPGgdly 469  Alistipes put...
Q9RYM8        322 r----------------dITVVVSAGNSyreewrn---paqlpgVIASAALdinndka---gfstygRHVSVAAPGv--- 376  Deinococcus r...
Feature 1                                                             #  #                        
1R6V_A        346 --------------------TILSTvpgedsigyeghnenvpatnggtYDYYQGTSMAAPHVTGVVAVLLQkfpn----a 401  Fervidobacter...
YP_001471655  369 -------------------fTILSTvplwdtye--------ffdqenpYALYGGTSMATPHVSGVIALLLEklgst--ea 419  Thermotoga le...
YP_001567802  370 --------------------DIISTipveslde-------yegidgnpYIRWSGTSMSAPYVSALAALIKEkcpd----a 418  Petrotoga mob...
ZP_03300901   445 -----------------gdgRIWSTttkl----------------agnYEYLAGTSMACPLVSGVAALVIEkygvgkkgf 491  Bacteroides d...
NP_809125     473 yynpvgqddpegweegihsgSILSTwiqn---------------gnatYGFMDGTSMACPHVSGVAALGLSyavkqrrhf 537  Bacteroides t...
YP_446403     406 --------------------AILSTwiagqg------------sveenFEFLSGTSMAAPHVSGAAALVVSefsd---vs 450  Salinibacter ...
ZP_02424566   470 yhk-----------nlsdagQVLSTvlkl----------------dgmYAYMSGTSMSCPHVSGVAALGLSyakqlgktf 522  Alistipes put...
Q9RYM8        377 -------------------dVMLASplfinadgtrk-tggytkdggsgYQLISGTSFSGPYTSGVAAVILGakpd----l 432  Deinococcus r...
Feature 1                     
1R6V_A        402 kpWQIRKLLENT 413  Fervidobacterium pennivorans
YP_001471655  420 tpYQIRKHIAAT 431  Thermotoga lettingae TMO
YP_001567802  419 nvYQIRKMIEVG 430  Petrotoga mobilis SJ95
ZP_03300901   492 tpETLKEILYQS 503  Bacteroides dorei DSM 17855
NP_809125     538 kaSEFVELLKSS 549  Bacteroides thetaiotaomicron VPI-5482
YP_446403     451 spEQVESILEES 462  Salinibacter ruber DSM 13855
ZP_02424566   523 epDEFRDMVLAS 534  Alistipes putredinis DSM 17216
Q9RYM8        433 dpHQVRRLMEET 444  Deinococcus radiodurans

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