1THM,1DBI


Conserved Protein Domain Family
Peptidases_S8_Thermitase_like

?
cd07484: Peptidases_S8_Thermitase_like 
Click on image for an interactive view with Cn3D
Peptidase S8 family domain in Thermitase-like proteins
Thermitase is a non-specific, trypsin-related serine protease with a very high specific activity. It contains a subtilisin like domain. The tertiary structure of thermitase is similar to that of subtilisin BPN'. It contains a Asp/His/Ser catalytic triad. Members of the peptidases S8 (subtilisin and kexin) and S53 (sedolisin) clan include endopeptidases and exopeptidases. The S8 family has an Asp/His/Ser catalytic triad similar to that found in trypsin-like proteases, but do not share their three-dimensional structure and are not homologous to trypsin. Serine acts as a nucleophile, aspartate as an electrophile, and histidine as a base. The S53 family contains a catalytic triad Glu/Asp/Ser with an additional acidic residue Asp in the oxyanion hole, similar to that of subtilisin. The serine residue here is the nucleophilic equivalent of the serine residue in the S8 family, while glutamic acid has the same role here as the histidine base. However, the aspartic acid residue that acts as an electrophile is quite different. In S53 the it follows glutamic acid, while in S8 it precedes histidine. The stability of these enzymes may be enhanced by calcium, some members have been shown to bind up to 4 ions via binding sites with different affinity. There is a great diversity in the characteristics of their members: some contain disulfide bonds, some are intracellular while others are extracellular, some function at extreme temperatures, and others at high or low pH values.
Statistics
?
PSSM-Id: 173810
View PSSM: cd07484
Aligned: 18 rows
Threshold Bit Score: 286.08
Threshold Setting Gi: 119717547
Created: 29-May-2009
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
 
active sitecatalytic triad
Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1                                                             #                          
1THM_A         2 TPNDPYFss---rQYGPQKIQAPQAWDIa--------------eGSGAKIAIVDTGVqsnhpd---------------la 49  Thermoactinomyc...
NP_242357    303 TPNDEFFke---yQWNLQQIDIEEGWSLa--------------sGENVKIAILDTGVdpnhpd---------------ik 350 Bacillus halodu...
CAD62181     413 LSHDIQYp----yQWPLKNNGENGGVKNadvkyepastllskrkLNDTLIAVVDTGVdstladl-------------kgk 475 Bacillus subtil...
YP_175254    306 EPNDEYFkp---yQWNLKQIHADDGWNFa--------------nGTDTTIAIIDTGVdpnhpd---------------lk 353 Bacillus clausi...
YP_924512      1 MTNDPLRs----qQWGLDQVRAEAAWATt--------------tGSGVVVAVVDSGVdlnhpdlqgqlvpgittvgcgpk 62  Nocardioides sp...
ZP_01910349  174 EPNDPMYp----lQWHLDAINTPEAWTHt--------------rGKGATVAVIDTGVawkdlrwknvda---ravpdlag 232 Plesiocystis pa...
YP_001306108 156 VPNDTYYe----yQWHYEAINLPKAWDIi--------------kSANVVVAVVDTGVsfthpd---------------lq 202 Thermosipho mel...
YP_001430374  20 SPTDPRFd----eQWALYKVGATCAWERtr-------------gSADVIVAVVDSGVdpthpdl--------------aa 68  Roseiflexus cas...
YP_001543964  28 PPNDPDAn----kQWVIRQLGLACAWEHlt-------------gNSDVTVAVIDSGVdmnhpdl--------------vd 76  Herpetosiphon a...
YP_001815109 236 LNNTGQRggvkgaDINFNAMQKRIAGKK----------------QSTVLVAVVDTGInptyadf-------------agr 286 Exiguobacterium...
Feature 1                                #                                                       
1THM_A        50 gkvvGGWDFVdndst---pqngNGHGTHCAGIAAAVTNnstGIAGTAPKA---SILAVRVLDNsGSGTWTAVANGITYAA 123 Thermoactinomyc...
NP_242357    351 dkivNGYNAVegnnn---fadkHGHGTHVAGVAAAVTNnvtGIAGISWKS---EILPVKVLNDnGEGSSFEVAKGIYWAT 424 Bacillus halodu...
CAD62181     476 vrtdLGRNFVgrnnn---amddQGHGTHVAGIIAAQSDngfSMTGLNAKA---KIIPVKVLDStGYGDTEQIALGIKYAA 549 Bacillus subtil...
YP_175254    354 gkltEGYNAIddssd---ykdtNGHGSHVAGIAAAVTNnidGVAGVSWQS---KIMPIKVLDEnGEGSSYSVARGIYWAV 427 Bacillus clausi...
YP_924512     63 qsycGDGSWVgmdga---aqpaDSHGTHVSGIVAAAADnglGVAGVARDA---KVMPIKALED-GSGSFGDIANGIRYAV 135 Nocardioides sp...
ZP_01910349  233 iefvHGETFVnnglp--eglddHAHGTHVAGTIAQATDnriGVAGVAHQA---KIMPLKVLGAsGGGSVASIANAIRYAA 307 Plesiocystis pa...
YP_001306108 203 gifvQGYDFVdndtdptdpaqdVSHGTHVTGTIAALSNngkGVAGVNWGGygiKIMPIRVLGAdGSGTLDAVAQGVRYAA 282 Thermosipho mel...
YP_001430374  69 rlrsDGYDFVdndad---prdeNGHGTHVAGIIAAILDnneGIAGLAPEV---TILPVRVMNArGKGSDRAIARGIRFAA 142 Roseiflexus cas...
YP_001543964  77 vlrtDGFDAVdgddd---psdeNGHGTHVSGIIAATINnskGIAGVAGGGt--RILPIRVMAAdGSGTNQDIIAGIRYAV 151 Herpetosiphon a...
YP_001815109 287 vrmdLGYDFVnrrqm---aiddHGHGSHVAGIIAANSNntyGMSGINAKA---SIIPIKVLGEdNYGLNSDIAKGILHGV 360 Exiguobacterium...
Feature 1                  ##                                                                    
1THM_A       124 DQGAKVISLSLGGtv---------gNSGLQQAVNYAWNKGSVVVAAAGNAGntapnYPAYYSN----AIAVASTDQNdnk 190 Thermoactinomyc...
NP_242357    425 DHGAKVINMSLGDyy---------hSDALRDAVKYAYDHDVVLIAASGNDNvedplYPAIYEE----VLTVAAVDDTrnr 491 Bacillus halodu...
CAD62181     550 DKGAKVINLSLGGg----------ySRVLEFALKYAADKNVLIAAASGNDGanslsYPASSKY----VMSVGATNRMdmt 615 Bacillus subtil...
YP_175254    428 DHGADVINMSLGDyy---------hSDLLYEAIQYADKHDVVIVSASGNDNseepmYPAVYPE----VITVAAVDQDrnr 494 Bacillus clausi...
YP_924512    136 DHGASVVNLSLGAvpggqalsltglDTAVTEAIAYAAAQGVLVVAAAGNESfpvcdTPSFEAG----ALCVTSTTRDetp 211 Nocardioides sp...
ZP_01910349  308 DNDADVINMSLGGpl---------pSRVMAKAVLYAHEKGVTVVCAAGNEKrsrvsYPAAYEG----SVAVAATDWSgkr 374 Plesiocystis pa...
YP_001306108 283 DHGAKIINMSLGGgg---------dSQILREAIQYAYNKGVTIVCAAGNENgp-vsYPAKYPE----TIAVASVRYDlqr 348 Thermosipho mel...
YP_001430374 143 DRGAKVINLSLGAtltln---adepSALVNDAIIYAQQQGALVVVAAGNDAv---pLPNAIAVdnpdVLVVAATDQRdhk 216 Roseiflexus cas...
YP_001543964 152 SKNVQIINMSLGSmlp-------ldSEDIVEAIKEADAAGVLVIIAAGNSFv---pLPNFAFGveefAMVVAATDPDdrk 221 Herpetosiphon a...
YP_001815109 361 KKGAKVINVSIGGaf---------sSRAIEDALAYAKKKGVLVVVASGNEGqnklsYPARSQY----AFSVGSTNRFdkr 427 Exiguobacterium...
Feature 1                                                                        #  #            
1THM_A       191 SSFSTYG---SWVDVAAPGs-------------SIYSTYpt-----------------stYASLSGTSMATPHVAGVAGL 237 Thermoactinomyc...
NP_242357    492 AFFSNFG---KHIDVTAPGe-------------HIPSLFpd-----------------nqYTVMSGTSMASPHAAGLAGL 538 Bacillus halodu...
CAD62181     616 ADFSNYG---KGLDISAPGs-------------DIPSLVpn-----------------gnVTYMSGTSMATPYAAAAAGL 662 Bacillus subtil...
YP_175254    495 SFFSNFG---DHVDLAAPGe-------------NIPSTYkd-----------------nqYAVLSGTSMASPHVAGLAAL 541 Bacillus clausi...
YP_924512    212 AWYSNGAikpDVDAVAAPGgaglv-----acadDIVSTVpvgtgsa--------acgqqdYDYYAGTSMATPHVAGVAAL 278 Nocardioides sp...
ZP_01910349  375 TFYSNWG---KQLDISAPGgdtrsdkngdghpdGVLQNTiriqd-----------psrndYLWFQGTSMASPHAAGVAGL 440 Plesiocystis pa...
YP_001306108 349 APYSNYG---PEVDVAAPGgdtsvdqngdgyadGVLSTAwtpn-------------ngdtYMFLQGTSMAAPHVAGVAAL 412 Thermosipho mel...
YP_001430374 217 APFSNSG---PWIAVSAPGv-------------NILSTMptyevfltsdqvprderfrrdYDYMSGTSQATPYVAALAAL 280 Roseiflexus cas...
YP_001543964 222 TDFSNYG---KWISVSAPGa-------------GIYSTMptydvymtsq-lpaeerfnknYDQMSGTSQATPVVAGLAAL 284 Herpetosiphon a...
YP_001815109 428 SSFSNYG---KGLDLVAPGq-------------DIPSYLsd-----------------gvSAFWSGTSMAAPHVVGVASL 474 Exiguobacterium...
Feature 1                                 
1THM_A       238 LASQG--RSASNIRAAIENTADKIS 260 Thermoactinomyces vulgaris
NP_242357    539 IRSLRpdLSNQEVMDIMKKTAKDLG 563 Bacillus halodurans C-125
CAD62181     663 LFAQNpkLKRTEAEDILKKTADDIS 687 Bacillus subtilis subsp. spizizenii ATCC 6633
YP_175254    542 LRSANpdLNNDQIGDIILGACDQLG 566 Bacillus clausii KSM-K16
YP_924512    279 LYAQG--RTAANVHDALVDTARTPG 301 Nocardioides sp. JS614
ZP_01910349  441 VVASGv-TNPKEVERIMKNTAVHPN 464 Plesiocystis pacifica SIR-1
YP_001306108 413 LYASGk-TTPEEIRAALKNTAKDLG 436 Thermosipho melanesiensis BI429
YP_001430374 281 LFSANpdWSAAQVAETIRTNATDIR 305 Roseiflexus castenholzii DSM 13941
YP_001543964 285 LFAQHpdWNADQVRAEIEKTADDIS 309 Herpetosiphon aurantiacus ATCC 23779
YP_001815109 475 LYSLKptIKASEVEAILKKSAQDLG 499 Exiguobacterium sibiricum 255-15

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap