Conserved Protein Domain Family
Peptidases_S8_CspA-like

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cd07478: Peptidases_S8_CspA-like 
Peptidase S8 family domain in CspA-like proteins
GSP (germination-specific protease) converts the spore peptidoglycan hydrolase (SleC) precursor to an active enzyme during germination of Clostridium perfringens S40 spores. Analysis of an enzyme fraction of GSP showed that it was composed of a gene cluster containing the processed forms of products of cspA, cspB, and cspC which are positioned in a tandem array just upstream of the 5' end of sleC. The amino acid sequences deduced from the nucleotide sequences of the csp genes showed significant similarity and showed a high degree of homology with those of the catalytic domain and the oxyanion binding region of subtilisin-like serine proteases. Members of the peptidases S8 and S35 clan include endopeptidases, exopeptidases and also a tripeptidyl-peptidase. The S8 family has an Asp/His/Ser catalytic triad similar to that found in trypsin-like proteases, but do not share their three-dimensional structure and are not homologous to trypsin. The S53 family contains a catalytic triad Glu/Asp/Ser. The stability of these enzymes may be enhanced by calcium, some members have been shown to bind up to 4 ions via binding sites with different affinity. Some members of this clan contain disulfide bonds. These enzymes can be intra- and extracellular, some function at extreme temperatures and pH values.
Statistics
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PSSM-Id: 173804
View PSSM: cd07478
Aligned: 59 rows
Threshold Bit Score: 323.03
Threshold Setting Gi: 162450862
Created: 29-Jul-2008
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
active sitecatalytic triad
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                    #                                                                    
BAB60711       97 LRGSGVVVAIVDTGIDYLNNEfmr--edETTRILrIWDQTIDSGKTpdgfisgseyteedin------kaiqaqkqgqnp 168  Clostridium p...
YP_001996901  158 YTGRNTIVAVVDFGFSALTRNgdfldadSNTRMLyVWDQNGSSPNPtepaftygrey---------------dstdians 222  Chloroherpeto...
YP_001613229  125 ADGEGVVVGVVDTGLDVTHPDfrd--kdGKTRIRwMLQAGSPKGLHaalehtygcddpdqsac---aifsdadidamlaq 199  Sorangium cel...
ZP_01906327    72 ERGAGCVVAVLDWGLDLLHPNfrr--pdGGTRLLaLWDQRAPRRAAaptspnpygrgrvfdrrtldaalqtadpfaaldl 149  Plesiocystis ...
YP_001378998  126 AQGQGVVIGVIDFGCDFVHRNfrn--pdGSTRLLaLWDQDGTPRAKgragygrvheradi----------dkalrakdpy 193  Anaeromyxobac...
ZP_01545838   137 RMGAGVLVGIIDYGMDFTLDDfrn--pdGTTRLAfLWDQRLDARQGerapddfaygveygad------qinqalnatdpf 208  Labrenzia agg...
NP_563478     115 LTGEGILVGFLDTGIDYTHNAfkd--deGNTRIEyIYDLENGVVYDknkineal---------------------ksedp 171  Clostridium p...
YP_001919516  107 LSGKGVLVGFIDSGIAYTHPAfmd--sdGNTRIEyIYDLSLGRKVYdketinkai--------------------ksndp 164  Clostridium b...
YP_001516005   98 QTGKDVLVGIVDSGFDLSHPMfrd--vsGNLRVVaLLDQTNGNQEYntsqlqt-----------------------qwga 152  Acaryochloris...
NP_349839      75 LTGKGVLVGFIDSGIDYTHPTfmn--kdGTSRILyIYDLSMGGKVWnnndinrai--------------------ksnnp 132  Clostridium a...
Feature 1                    #                                                              #     
BAB60711      169 ydiVPSKDDYGHGTKMAGIVGargin-------reivGVAPDCEFIIIKLQeaskeyvdfyyakgdkakyrntdIIMALK 241  Clostridium p...
YP_001996901  223 agnLSFGSGYTHGTNCVGIAAgdgsa-------sgqkGMAPDAKIILVALKsggg----------------sseLVDAFN 279  Chloroherpeto...
YP_001613229  200 gkpGILRDIAGHGTHVASIAAgnggpmv--serpryvGVAPAATLIIAAPArpgeg-------------fddpdILKAVQ 264  Sorangium cel...
ZP_01906327   150 dprRVAPRGGSHGTHVLDIAAgrpv--------vgpgGVAPGAELVFVEMGpsntagvy--------nfgdelaVFEAVH 213  Plesiocystis ...
YP_001378998  194 aalRYEPEPGSHGTHVTDIAAgngrg-------tgtpGVAPNADLVFVELSagdlpwdgpdv--vgrtfgdsvhLVEALK 264  Anaeromyxobac...
ZP_01545838   209 svvRHRAEAGSHGTHVAGIAAgngrshddnfaegthlGAAPEATLIFVQPDtrdgsnt----------ftdsshVVDAVA 278  Labrenzia agg...
NP_563478     172 fsiVPEIDLSGHGTHVAGIACaggni------nfdnyGVAYKSSIAMVKITgenslr-----------aalstqLMRGLK 234  Clostridium p...
YP_001919516  165 ysiVPVLDNTGHGTHVAGIACaggki------nsvykGAAPEASIAMVKAArgswi--------------lssqIMQGIK 224  Clostridium b...
YP_001516005  153 ggsRPGEDKDGHGTHVASIAGgskl--------dqyeGIAPDAKFLLVKTDft--------------------nVDLAVS 204  Acaryochloris...
NP_349839     133 asiVPERDQLGHGTHVAGIACgggni------drryfGPAYESNIAMVKMTgegkta-----------ygkstqLMRGVK 195  Clostridium a...
Feature 1                           #                                   #                         
BAB60711      242 YLYELSFtlnkpMIIYLPLGsnlgdHAGASILERYVDTKISgr-nsLFVVTSTGNQgnt--------dtHTSGEIKSNGD 312  Clostridium p...
YP_001996901  280 YITAKAAlhnlpVSVNYSAGsnfgaHDGSRDSERAISGYAGp---gKLFAVAAGNNrg---------qnVHVEGDVPNGG 347  Chloroherpeto...
YP_001613229  265 FIFDRAEelnmpAVVNLSVGsdfgpHDGTSPLEAGLAAMVGsahpgRAIVVAAGNSgatyeidgegplgIHTEAHVSAHA 344  Sorangium cel...
ZP_01906327   214 FAIAAAGsr--pLVINMSMGrqagpHDARTLVEMALDAAVRsr-pgLVIVNSAGNYada--------aaHGSGRFEPGEL 282  Plesiocystis ...
YP_001378998  265 FIFDRAGer--pCAVNISLGtnggpHDGSTLVEQAIDALVAar-pdRAVVIAASNAya---------dgIHASGVVRAGK 332  Anaeromyxobac...
ZP_01545838   279 YIMGKADaldmpCVINMSLGqnggsHDGESLVERAIDRLLEpl--gRAFVSAAGNEhv---------whGHAAGRIKTGE 347  Labrenzia agg...
NP_563478     235 FLMDKSNeinkpLVVNISLStndgsHNGSSLLEKYIQTFTQlq--kAVIVVAAGNEg-----------nSAHHVGGKMKK 301  Clostridium p...
YP_001919516  225 FLIDKSKeinmpLVINMSLStnngaHNGSSLLEQYISTVSNle--rATIVIAAGNEgd---------aaHHTGGELDQTQ 293  Clostridium b...
YP_001516005  205 WIYDQADgn--pCVVNLSLGyhfgaHDGTSNEERLYDTLVGp---gKILVVSAGNErnd--------siHLNGMFVPNQL 271  Acaryochloris...
NP_349839     196 FLVDRANsmnmpLVINLSFStndgaHDGTSLLEQYIENICLle--kISFVISAGNEgd---------mgHHVGGVLRESQ 264  Clostridium a...
Feature 1                                                                                         
BAB60711      313 SQIIELNCGKEQQGLvl--------------qiYAQRPSKi--kLGILSPSGERFENTNprktkhilindaptwkfiyeg 376  Clostridium p...
YP_001996901  348 QDEVSFNTGSGQFYAh----------------iWYDGSDSl--lAYLIAPGGSPIYGPFslsenv----------ysedg 399  Chloroherpeto...
YP_001613229  345 ETRVPIRTPRAERGEgy---------------vWITFRPGdevsVGLDGPGGEAWIGLIepgddag-------ytgddge 402  Sorangium cel...
ZP_01906327   283 GARRELTWTLRPGDAtgde-----------lelWYPGADRl--rVQLVDPHGHVHGPWRlgergaivd----prrhglar 345  Plesiocystis ...
YP_001378998  333 TADVSWEISRGDDTSnev-------------elWYDGDDRf--dVELIGPDGESAGRVAlgesat---------arskgr 388  Anaeromyxobac...
ZP_01545838   348 SRELRWKTGGGMPLPdgsvtqtgtdrtsseleiWYSSVDRf--aVTLIAPDGTQYGPVNpgks--------------llq 411  Labrenzia agg...
NP_563478     302 EEDLDLNIGDGEKGIild-------------ffKPVLVDVs---VEVISPTGISTGPMGlsesyk---------erfvgr 356  Clostridium p...
YP_001919516  294 TKAFNVASDESTVVInl---------------yKSILPDVs---LNIISPTGQSSGNINiqegyfh-------ggigrdr 348  Clostridium b...
YP_001516005  272 EEVVFDILPRNSAGVppfv----------attfWYPDNDDy--eLDLITPSGQALPTPTlgntdsysa---gnlsieisr 336  Acaryochloris...
NP_349839     265 TISMNIAEGERSIILq----------------lYKSFTQDi--aVEVKNPANVSTGVINikegyn---------egriqn 317  Clostridium a...
Feature 1                                                                                         
BAB60711      377 TTVQVTYDSPDEFTGddkfvik------megiteGVWRFILTGNni-vdGKYYAWILQREllae-------ntrFLNPSP 442  Clostridium p...
YP_001996901  400 GNILVYHNNYEPNGDreiqfll------qnhngeSGWKLRLADVgg-deVHFDGWKLSYGs------------wASHTTS 460  Chloroherpeto...
YP_001613229  403 TTGAVINRLANGKSPitaatns------avvafsGAWKAGEFAIrlrgrGDAQLWVTGLGdvspsh---dlgllFPRAIK 473  Sorangium cel...
ZP_01906327   346 TVWGRAYHRADDPNNgdhlfnlf----ldpqapaGPWTLELEAEhi-vdGRYHAWIERDAggae------qqsrFVPEDV 414  Plesiocystis ...
YP_001378998  389 TVLFAAHRAKDPNNGdnvvnvf-----ldvgappGPWTLRLHGAdv-kdGRFHAWIERDDagp---------ssFAARPL 453  Anaeromyxobac...
ZP_01545838   412 DEVFIDSERFSSLNGqariymqleapfglgmlrsGVWTVKLEALeg-enGQFDAWIERDLrdknnnyadqsffqGGDFDP 490  Labrenzia agg...
NP_563478     357 EKIVVYSTGPKPFDIqgqttisil--plgdtitsGGWRIIVRKLnn-ydGYFDVWLPIAEglne-------rtrFLQPSV 426  Clostridium p...
YP_001919516  349 YDIYVSGPKPFELNSeiqiiiss---isseylieGVWKLQITTLnn-ykGSFSIWLPISEglnp-------ktkFLEPIN 417  Clostridium b...
YP_001516005  337 TAYTISDLTQIQISIsfsnfs-------ippgslQNWRFRIRCVsa-ttGRFDAWFHNSGfar--------frsHPLVQA 400  Acaryochloris...
NP_349839     318 DRYFIYYSGPKPFSLngeilinlv--sdneilttGTWMITITKRsk-tvGNYDIWMPVTEglnp-------ktrFLRPNV 387  Clostridium a...
Feature 1                                                                                         
BAB60711      443 YTTLTIPgta--ktIINTSYYnqnng-----------------------aivseSGRGYTmk-dyIQPIITAGGINAItt 496  Clostridium p...
YP_001996901  461 YKNITTPsta--dsAISVAAYsvgsg-----------------------tiysgSSVGPTrt-nlPKPEITAPTDVSTt- 513  Chloroherpeto...
YP_001613229  474 QGTINVPash--pgLLAVGCTinrvrwqpqgsstsvrltsldgvdieedsacyfSAAGPTpf-gvAKPEISAPGGLVAaa 550  Sorangium cel...
ZP_01906327   415 DRRGTTGtianghaTLAVGAWdpktg-----------------------apatfSSGGPTrd-grQKPELLAPGVDQLaa 470  Plesiocystis ...
YP_001378998  454 DNSHTLGsiscghhSIVVGSFdahraa---------------------apisffSSAGPTrd-grHKPEVSAPGHQVVaa 511  Anaeromyxobac...
ZP_01545838   491 VRTLGTPatt--rrAIAVANYshhsl-----------------------apaasSSRGPTrd-nrYKPEVAAPGTNIVas 544  Labrenzia agg...
NP_563478     427 YNTLGIPatv--egVISVGSYnflnn-----------------------nlsafSGRGVVrpewlIKPDLVAPGENILst 481  Clostridium p...
YP_001919516  418 YNTLGIPatv--dnIIAVGSYnsltn-----------------------tlssfSGRGPQiqsniIRPDLVAPGVGILgp 472  Clostridium b...
YP_001516005  401 QGTIGIAata--rgCIAVASHvsknswdsddgpq-------qdlmavmgrsssfSSLGPTrd-grLKPEISAPGQYITaa 470  Acaryochloris...
NP_349839     388 YDTLGIPgtv--rdAVTVGSYnyltg-----------------------aissfSGRGREtg-npIKPDVIAPGENIEss 441  Clostridium a...
Feature 1                                                #                                        
BAB60711      497 kp---------------------------gggtITMSGASVAGAILAGCCALIIQWavvdgndpqmyatkLQAYIIRGAR 549  Clostridium p...
YP_001996901  514 --------------------------------aGSFTGTSAAAPHIAGLGAILLQArpalsv---sefmaAITDSARTDG 558  Chloroherpeto...
YP_001613229  551 masgvdprddpgglfdtpgcpgdepcfvvddhhAIASGSSMAAPQVAGAIALLFQVnpnl------tqaeVTEALQAGAR 624  Sorangium cel...
ZP_01906327   471 asftg----------------------gaaprlTRKSGTSMAAPHVAGTVALLLARaphl------dvetVRALLRRSAR 522  Plesiocystis ...
YP_001378998  512 sar-------------------------ssdgaIAMSGTSMASPAVTGLAALVLAEaraagr--slsieeLRAIVLSAVT 564  Anaeromyxobac...
ZP_01545838   545 gaqggrsn----------------gnggvhpvrVPKSGTSMAAPHVAGIVALLFQEarsl------sapqVANILMATSD 602  Labrenzia agg...
NP_563478     482 ve---------------------------eqgfDTKSGTSMAAPQVSGICALLFEWgiirnndpflygerIKYYLIKGAK 534  Clostridium p...
YP_001919516  473 ip---------------------------nggyDSKTGTSMAAPQVAGICALIMQFgivngndpylfgqrLKYYLVRGAN 525  Clostridium b...
YP_001516005  471 laensdlsd-------------vsvradetnslLTIEGTSMSAPVVTGAIALMLQKnpsl------tpeqVKEILKESAK 531  Acaryochloris...
NP_349839     442 tp---------------------------gggyDALSGTSMASPTVSGICALLMQWgivlkkdiylygdrLKYYLLNGAK 494  Clostridium a...
Feature 1                          
BAB60711      550 kre--gdtYPNRQWGYG 564  Clostridium perfringens
YP_001996901  559 atg--nipPHNNSWGFG 573  Chloroherpeton thalassium ATCC 35110
YP_001613229  625 ypr--gavPIEAQLGPG 639  Sorangium cellulosum 'So ce 56'
ZP_01906327   523 pv-----pGPRAFVGWG 534  Plesiocystis pacifica SIR-1
YP_001378998  565 pag---aaGWDARYGNG 578  Anaeromyxobacter sp. Fw109-5
ZP_01545838   603 npd--gtpGFDVAWGFG 617  Labrenzia aggregata IAM 12614
NP_563478     535 rtif-geaYPNPDLGYG 550  Clostridium perfringens str. 13
YP_001919516  526 rart-dieYPNPLWGYG 541  Clostridium botulinum E3 str. Alaska E43
YP_001516005  532 kdahtqdtAWTPAYGYG 548  Acaryochloris marina MBIC11017
NP_349839     495 kdre-ditYPNPSFGYG 510  Clostridium acetobutylicum ATCC 824

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