1D8H,1D8H,2QY2


Conserved Protein Domain Family
CYTH-like_mRNA_RTPase
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cd07470: CYTH-like_mRNA_RTPase 
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CYTH-like mRNA triphosphatase (RTPase) component of the mRNA capping apparatus
This subgroup includes fungal and protozoal RTPases. RTPase catalyzes the first step in the mRNA cap formation process, the removal of the gamma-phosphate of triphosphate terminated pre-mRNA. This activity is metal-dependent. The 5'-end of the resulting mRNA diphosphate is subsequently capped with GMP by RNA guanylytransferase, and then further modified by one or more methyltransferases. The mRNA cap-forming activity is an essential step in mRNA processing. The RTPases are not conserved among eukarya. The structure and mechanism of this fungal RTPase domain group is different from that of higher eukaryotes. This subgroup belongs to the CYTH/triphosphate tunnel metalloenzyme (TTM)-like superfamily, whose enzymes have a unique active site located within an eight-stranded beta barrel. The RTPase domain of the mimivirus RTPase-GTase fusion mRNA capping enzyme also belongs to this subgroup.
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Statistics
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PSSM-Id: 143621
Aligned: 28 rows
Threshold Bit Score: 77.0631
Created: 1-Oct-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 14 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:This active site includes the metal-binding and gamma-phosphate binding sites.
  • Comment:Based on the Saccharomyces cerevisiae mRNA-RTPase structure and mutational studies.
  • Structure:1D8H_A; Saccharomyces cerevisiae mRNA-RTPase monomer bound with sulfate and manganese ions, contacts determined at 4.5A
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                        # #                                             
1D8H_A        45 KSVQDWVYATIYSiape----------lrsfIELEMKFGviidakgpdrvnppvss--------qcvfteldahltpnid 106 baker's yeast
2QY2_A        22 NEIVEMLRDYSNGd----------------nLEFEVSFKni--------------------------------------n 47  Mimivirus
XP_820839     26 KALFDAVNKHLSNp----------------fIEVEMRLGqfkveedalrentsdqng-----aqtnglrivdanftacvs 84  Trypanosoma cru...
XP_636333     53 NRIIELSKQHGGId----------------sIEVEGRIGlfsntsn----------------------------gntfkp 88  Dictyostelium d...
XP_843813     34 RTIVEELFQRLQSldh-------------thMELEARLCrraevsdksgstsserew------kgrrarktrtevmpgva 94  Trypanosoma bru...
XP_002289909  52 TQPEEAIKMFLIQarstarrrppqyggspppIEIEARLGtilspfgahdmral--------------ssgaklipikgvn 117 Thalassiosira p...
XP_001565670  69 LEVAESLLARLKPflr------------eshIEIEARICsiiqskkrthsmmkghv--------gatattissrdaphiv 128 Leishmania braz...
XP_001610428  35 GPLAAALDNGDFDstsgy---------ppceLEIEVRLGsirnvrdgqrftlpsat-------dalisdrafvrfqpsvt 98  Babesia bovis T2Bo
XP_765772     50 GPLFEALTNNHFIksgynmnsgsvfskpecqLEVEVRLGfiqnirsehrftlpvtt-------dtllasrapvkfvpgvt 122 Theileria parva...
XP_002139113  21 ITEVESIAQALVDlvegfpei--ledncnidLEIEGRIGtimsdtsreriklpcegltllapsdkdfpyrfvpgvqkwqm 98  Cryptosporidium...

Feature 1                                              #                                         
1D8H_A       107 ASLFKELSKYIRGIsevte------ntgkfSIIESQTRDSVYRVgls--------------------------------- 147 baker's yeast
2QY2_A        48 YPNFMRITEHYINItp------------enKIESNNYLDISLIFp----------------------------------- 80  Mimivirus
XP_820839     85 TEDYERIKTYLMTE----------------MENSSMTRSVTHDVs----------------------------------- 113 Trypanosoma cru...
XP_636333     89 GMVRDDWNDLYHHLk--------------lKSEPVATTETDYIY------------------------------------ 118 Dictyostelium d...
XP_843813     95 EEDYKRIEEFCLDK----------------GKEGSVTRSTTRDVs----------------------------------- 123 Trypanosoma bru...
XP_002289909 118 RVVHAFVCNISGEGnsnant----tsaaktNFEGGITRSNYLKWtnaglselgpltnafsikhknpnesesaaikfqlae 193 Thalassiosira p...
XP_001565670 129 EGEHRRIQVGVSAEdfarmkaymaekpealPFQESVTEDVNTRAg----------------------------------- 173 Leishmania braz...
XP_001610428  99 KSQFQSAFTLIKQIaeergt-----sdewdTQLGILTFDRFFKLpe---------------------------------- 139 Babesia bovis T2Bo
XP_765772    123 EDQYNAARNFLIETseeddp-----knewtTCLNSCTINRFFEIqgy--------------------------------- 164 Theileria parva...
XP_002139113  99 QAVIDELKRNLEETki-------------pYKVNTRNTSDYYVLnsens------------------------------- 134 Cryptosporidium...

Feature 1                      #                                     #   #                       
1D8H_A       148 -------tqrPRFLRMSTDIktg--------------------------rvgQFIEKRHV--AQLLLYSpkd-------- 184 baker's yeast
2QY2_A        81 ---------dKNVYRVSLFNqeqigefitkfskassndisryivsldpsddiEIVYKNRGsgKLIGIDNw---------- 141 Mimivirus
XP_820839    114 ----------LRGWRHTYATdedgn----------------------ptrcvSIVRKKRL--FVKNIVVplg-------- 151 Trypanosoma cru...
XP_636333    119 ----------SDSIRVAYDEhs-----------------------------kKCLRKDKKt-DKTSFDQstnl------- 151 Dictyostelium d...
XP_843813    124 ----------FGQWRYTYTSgkps-------------------------eciACIRKERL--FVLDVPVpsg-------- 158 Trypanosoma bru...
XP_002289909 194 vesvetvfcyPNSTRINFPHnppqsn---------------------vltkgHQEKKEKL--SVIDIALpaa-------- 242 Thalassiosira p...
XP_001565670 174 ----------RYTYAIANDGses---------------------------fiGCIEKRRL--CNVEVRVpgc-------- 206 Leishmania braz...
XP_001610428 140 ---------yEEPVRISVPQnqtkg-----------------------aksfEAIRKVKL--LTWNVSTgssmrdtqmnd 185 Babesia bovis T2Bo
XP_765772    165 --------deLVRISVSNDSnspvnr--------------------qqkepvDAIRKVNL--LTWNISSgsteepseese 214 Theileria parva...
XP_002139113 135 ------shsdKSLIRVSFISpktat-----------------------dkpvDAIWKEKI--AIFDFYSpgynndidgh- 182 Cryptosporidium...

Feature 1                           #                       # # #              #                 
1D8H_A       185 ---------sydVKISLNLELPvpdndppe---kyksqspISERTKDRVSYIHnd--scTRIDITKVENhnqns------ 244 baker's yeast
2QY2_A       142 -----------aITIKSTEEIPlvagkskiskpkitgserIMYRYKTRYSFTInk---nSRIDITDVKSspiiw------ 201 Mimivirus
XP_820839    152 ---------aynLRFAVSTETPgdlrfs------gagpraGHTRLKDRLSITDg----mFRYDMTQVTEk---------- 202 Trypanosoma cru...
XP_636333    152 ---------iydFRISTSIEEKfpppls-------lppgyIIRREKQRYTFTEd----qWKIDLTKVIVrpdfn------ 205 Dictyostelium d...
XP_843813    159 ---------aydIRFSACTEITgelpsp------nfaptrGYTRHKDRLSVTDg----lFRYDLTRVRDk---------- 209 Trypanosoma bru...
XP_002289909 243 ---------pydLRLQLATEVPmgegnnipn-vksiphgwNKKRVKRRRSYSRldnsfaWKIDVTEVTStdapaaggasa 312 Thalassiosira p...
XP_001565670 207 ---------pydIRVSVSTEVPkpaida------pavkphGCVRRKRRWTATEk----tFEYAFTRVSTvd--------- 258 Leishmania braz...
XP_001610428 186 dnddvgdqeildYRIAVNLEYRvpirdl------pntsaaTTRRKKNRNTYINkk--ghLRFDLTQVQTvesdss----- 252 Babesia bovis T2Bo
XP_765772    215 --efstglegldYRIAVNLEYKvpltnl------pttsnaKYCRRRLRDSFIHte--skMRFDLSKVEDvten------- 277 Theileria parva...
XP_002139113 183 --cnytdqtfkdFRIAINIEHKenptlimnslvgqienvcVLERRRNRETLDVs----tFILDATNVTQiesrr------ 250 Cryptosporidium...

Feature 1                        # # #                                      
1D8H_A       245 -------ksrqsETTHEVELEINtpallnafdni----tndskeyaslIRTFLNNGTII 292 baker's yeast
2QY2_A       202 -------klmtvPSNYELELELInk---------------------idINTLESELLNV 232 Mimivirus
XP_820839    203 -----------gVLMHEVEIEGVfsshek-------------qlteswLEELLRRAMRL 237 Trypanosoma cruzi strain CL Brener
XP_636333    206 --------atveQELYEVEIELFpeaiqacq---------ektsltelLNDFLNAIKGL 247 Dictyostelium discoideum AX4
XP_843813    210 -----------hTTGYEVEVEFLlkdndek------------kitdshVEELVGRALSL 245 Trypanosoma brucei TREU927
XP_002289909 313 aaasmkgsgqhaDIGYEVEMELSssmt------------------qklIQTQDEAAARK 353 Thalassiosira pseudonana CCMP1335
XP_001565670 259 ----------hkYPVFEVEVEGIhascqa-------------gvtkawLADLLGRLVAL 294 Leishmania braziliensis MHOM/BR/75/M...
XP_001610428 253 -------vtddnNAQYEVEAELNggviinllkstglsydkkiqllkhhCSTLIRSVRRL 304 Babesia bovis T2Bo
XP_765772    278 --------kdptTSKFEIEIELLgeeivkslmdkslssdqrmmnlkyhCSTLIRSSRYI 328 Theileria parva strain Muguga
XP_002139113 251 --------gyrhNETYELELELKsshflp-------------llksyvKELNNGNLKII 288 Cryptosporidium muris RN66

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