1UG9,1LF6,1LF6


Conserved Protein Domain Family
GH15_N

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cd07430: GH15_N 
Click on image for an interactive view with Cn3D
Glycoside hydrolase family 15, N-terminal domain
Members of this family are N-terminal domains uniquely found in bacterial and archaeal glucoamylases and glucodextranases. Glucoamylase (glucan 1,4-alpha-glucosidase; 4-alpha-D-glucan glucohydrolase; amyloglucosidase; exo-1,4-alpha-glucosidase; gamma-amylase; lysosomal alpha-glucosidase; EC 3.2.1.3) hydrolyzes beta-1,4-glucosidic linkages of starch, glycogen and malto-oligosaccharides, releasing beta-D-glucose from the non-reducing end. Glucodextranase (glucan 1,6-alpha-glucosidase; exo-1,6-alpha-glucosidase; EC 3.2.1.70) uses an inverting reaction mechanism to hydrolyze alpha-1,6-glucosidic linkages of dextran and related oligosaccharides, releasing beta-D-glucose from the non-reducing end. These N-terminal domains adopt a structure consisting of antiparallel beta-strands, divided into two beta-sheets, with one sheet wrapped by an extended polypeptide, which appears to stabilize the domain. The function of these domains in the enzymes is as yet unknown. However, it is suggested that domain N of bacterial GA is involved in folding and/or the thermostability of the A domain that forms an (alpha/alpha)6-barrel structure.
Statistics
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PSSM-Id: 143632
View PSSM: cd07430
Aligned: 42 rows
Threshold Bit Score: 227.488
Threshold Setting Gi: 222479924
Created: 4-Oct-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Domain
Conserved site includes 29 residues -Click on image for an interactive view with Cn3D
Feature 1:Domain interface
Evidence:
  • Structure:1UG9: Arthrobacter globiformis glucodextranase interface between N domain and A domain, including linker region; contacts 4.0A
    View structure with Cn3D
  • Structure:1LFG: Thermoanaerobacterium thermosaccharolyticum glucoamylase interface between N-terminal domain (all beta-strand) and C-terminal domain (all alpha-helical), including the linker region; contacts at 4.0A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                  ######## ## ##        ## # #        # #   ##                           
1UG9_A          6 PGSPGa--aATWTKGDKeGVGTSln----paSKVWYTLTEGtm-sEVYYPhADTPNTRELQFAVSd---gTSAQRese-- 73   Arthrobacter ...
YP_136535     694 TEGPSe--rPTWTTGEKyGIGTVadhdeqdpSRVWFTLTEGal-tEARYPqVDMMNLRTLDFLVRctdetDYAVRthren 770  Haloarcula ma...
Q7NSD6         30 FGSPGg--aPVRGPAAKsFVGTAvn----spSRVYFTGYRGiv-sEVYYPlLDTPESVDLQFLVGda-grTFVDEekr-- 99   Chromobacteri...
ZP_01465400    44 PGHPGa--pALWTPANKaGFGTArs----aaSRVWYTLGSRgdltEVYYPtLSTPSVRELRFVVSdg--rTFAELese-- 113  Stigmatella a...
YP_001108404   34 PGAPGt--dPGYAGADKlGFGTSrd----aqSPVWFTLGRGga-aDLYYPnLSTPASRQLQVVVTdg--aGFVQRlsd-- 102  Saccharopolys...
YP_001614668   33 AKGGGv--pSVWGPAEKsFLGTStt----atSRVYFTGHRGiv-sEVFYPvLDTVNTVDLQFLVGdt-akTFVDEekq-- 102  Sorangium cel...
ZP_02381230    15 AGSPSaqarGVWNPANKhGFGTStt----leSKVWFTLGSTgl-dEIYYPrLDTPSVRDLRFVVSdg--eTFAERvtd-- 85   Burkholderia ...
YP_001834477   27 PGAPGy--kQGWSPANKqGFGTStt----heSNVWFTLGGSgl-tDVYFPrIDTPSIRDLQFVVSdg--rTFAERese-- 95   Beijerinckia ...
YP_002566161  727 PRGPDe--aPHWTTGRKfGVGTPadhkaadpVPVWFTLTEGal-tEARFPrIDVMNVRTFDFLVAdp-etGYTVRtfdet 802  Halorubrum la...
ZP_03870886   528 IEGPTe--rPTWTTGEKfGLLTAadhyeddpSRIWCTLTRGav-tEVRFPrVDLMNLRTLDFLIRss-dgEYTVRthret 603  Halorhabdus u...
Feature 1                   # #         # #                                                       
1UG9_A         74 -----qttRTVELAdp----kaLSYRQTTTdn----agRWRLTKTYVTDPrrSTVMLGVTFEVldg---gdYQLFVLSDP 137  Arthrobacter ...
YP_136535     771 rrtddsvrRRVEPVdd----eaLLYRHVFTetgdgqghEWTLTVDYATDPehDAIVADVSFSSadd---ksYDVFSVADI 843  Haloarcula ma...
Q7NSD6        100 ------qaYSASQSdk----rtMSWVASTGna----ghNWQISKRIFADPnrDALVQRVTFTAlnghtvgdFNLYALFKP 165  Chromobacteri...
ZP_01465400   114 -----ateHRVELAdp----rsLTFRQVNTar----sgRYRLTKTYVTDPerHVVLIGVRFESlsg---epYSLHVVYDP 177  Stigmatella a...
YP_001108404  103 ------vpTRTEPVdp----ktPSYRQVSTg------qGWEATATYVTDParPSVVVDLQVRSldg---kpLQTYVLHEP 163  Saccharopolys...
YP_001614668  103 ------qsFTVTQPdk----rsMRWQATTTns----ahNWRITKTVFTDPgrHALIQRVRFDAlggrnvtqFNLYLLHNP 168  Sorangium cel...
ZP_02381230    86 -----hatHETTLIda----dgLTYRQVDVdn----agRYRLTKTYVTDParPVVLVDVTFESldg---rpYQVYALLNP 149  Burkholderia ...
YP_001834477   96 -----atrYHVELAap----hsLVYRQINTan----nkRYRLTKTYSTDPerAVLLVDVTFESldg---hpYQLYALLNP 159  Beijerinckia ...
YP_002566161  803 s--hvtatETVERTteptvddaLAYTQTICetgdghghSWTLTVEYAVDTdgDAILADVAFEGsr-----eYDVYALVDT 875  Halorubrum la...
ZP_03870886   604 rraddsveRRVEPLdd----esLRFRHVFVetgdgrghEWELTVEYAVDPahDALLADVDFEAadg---edYDLFAVADT 676  Halorhabdus u...
Feature 1         #                      #                                                        
1UG9_A        138 Sla------------------gtSGGDTGSVTd----gALLASdlad---------------aatpVATALVssv----- 175  Arthrobacter ...
YP_136535     844 AltstitedrairygqpgsyhlvGRNPRAYTDqtd-ndLLVDEngd-----------------aysIAVAMAaag----- 900  Haloarcula ma...
Q7NSD6        166 Yld------------------nsGSGNSAQTVavaggyALAASrn--------------------sRASALMaslpwktv 207  Chromobacteri...
ZP_01465400   178 Sls------------------ndGMDDVGTSQe----gALLASdg--------------------tSASALLaap----- 210  Stigmatella a...
YP_001108404  164 Tlt------------------kdGSDDRSRTEg----aTLVASdr--------------------sAASALRatp----- 196  Saccharopolys...
YP_001614668  169 Amd------------------nsGAGDTSRTVdaggrtLLVASen--------------------sRASALAasrpwkvv 210  Sorangium cel...
ZP_02381230   150 Apg------------------naLTNTTGETNg----aTLTARng--------------------nVATAFAssp----- 182  Burkholderia ...
YP_001834477  160 Apg------------------ndQMHTHGETFh----hALVASng--------------------nVASAFTadp----- 192  Beijerinckia ...
YP_002566161  876 Tit------------------nvGSNDRGDRVddsdgyHLLARnndaaerstgk--lvdddgdsfeVALAIDsdd----- 930  Halorubrum la...
ZP_03870886   677 Slt------------------ntGTAERGLRLgepgahHLVARdptaytgetddpllvdeegeaysVAMAMTatt----- 733  Halorhabdus u...
Feature 1                                                #                                        
1UG9_A        176 -gfgAVANGYvgtSDGWTDLaadgr---ldnaSATAGpGNISQTGQIPlaag--gkteFSLALGFGad--taEALATAKA 247  Arthrobacter ...
YP_136535     901 -rfdWATVGAa-gSDRLDGLysdgd---lpdpVESIDgTNLVLIGRLDsgt----rvrDTVALGFArqadtaAALGEADG 971  Haloarcula ma...
Q7NSD6        208 ngqaMLSSGFvgqSDGWTDLigsgdn-tmnwtYGSATnGNVAQTGWLDlgdptatsvsFDMVLAFGkn--qgDAVNAAAA 284  Chromobacteri...
ZP_01465400   211 -altRTSSGYlgvSDGWSDLqdnfq---qdwaYASAApGNVVQTAQLEvdgr--drqqVTLALGFGtt--pdEARVAARD 282  Stigmatella a...
YP_001108404  197 -aftETSSGYlgaSDGWTDLsrhkr---ltahHPEAGaGHVGQVGRLPvdgv--gtshAVLTLGYGkh--asEAVATAEE 268  Saccharopolys...
YP_001614668  211 sgapMVSSGFvgeSDGWTDLlggnvdrtmtwrNDLASgGNVAQMGWIDlgasavtsvsFDVVLGFGat--eiEAAATANA 288  Sorangium cel...
ZP_02381230   183 -pftRLSNGHagaSDGWQDIgrhfr---mtwtYACAPrGNVVQTGHTPldgv--trthVTLAIGFGad--taQAASAAQC 254  Burkholderia ...
YP_001834477  193 -pftRTSNGYlgvSDGWQDLsshhh---mewtYVVADqGNVVQTGQTTldgv--trkhLTIAIGFGst--ttQAASQAQD 264  Beijerinckia ...
YP_002566161  931 -gfeWASVLAa-gSDKAGALfadgd---rgegTETAT-GNVVLAGLVGsgt----evsDAVALGFAenadtaAALGEARG 1000 Halorubrum la...
ZP_03870886   734 -rfdWATVAAa-gSDHLKDLfadge---lpppKDDVDnENIVLLGRIGsge----rtvETLALGFAryadtaAALGEATG 804  Halorhabdus u...
Feature 1                         #  #
1UG9_A        248 SLgtgykkVSKSYTGEWKKY 267  Arthrobacter globiformis
YP_136535     972 ALdrgyetVSAEYADSWGDF 991  Haloarcula marismortui ATCC 43049
Q7NSD6        285 ALgsdlsaAQQQYDNAWHAY 304  Chromobacterium violaceum
ZP_01465400   283 SLasgfeqVAERYAAGWHGY 302  Stigmatella aurantiaca DW4/3-1
YP_001108404  269 TErtgfeaAARAYGEGWHRY 288  Saccharopolyspora erythraea NRRL 2338
YP_001614668  289 TLgsnlstLQTTYDNGWKSY 308  Sorangium cellulosum 'So ce 56'
ZP_02381230   255 TLadgfasTARLYAEGWHRY 274  Burkholderia ubonensis Bu
YP_001834477  265 ALrtgfdrIAQAYAYGWRRY 284  Beijerinckia indica subsp. indica ATCC 9039
YP_002566161 1001 AIsrgfadISESYVETWREW 1020 Halorubrum lacusprofundi ATCC 49239
ZP_03870886   805 ALdrgygmAEAAYDDTWAAF 824  Halorhabdus utahensis DSM 12940

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