1Z2W,1W24,2A22,2R17


Conserved Protein Domain Family
MPP_Vps29

?
cd07394: MPP_Vps29 
Click on image for an interactive view with Cn3D
Homo sapiens Vps29 and related proteins, metallophosphatase domain
Vps29 (vacuolar sorting protein 29), also known as vacuolar membrane protein Pep11, is a subunit of the retromer complex which is responsible for the retrieval of mannose-6-phosphate receptors (MPRs) from the endosomes for retrograde transport back to the Golgi. Vps29 has a phosphoesterase fold that acts as a protein interaction scaffold for retromer complex assembly as well as a phosphatase with specificity for the cytoplasmic tail of the MPR. The retromer includes the following 5 subunits: Vps35, Vps26, Vps29, and a dimer of the sorting nexins Vps5 (Snx1), and Vps17 (Snx2). Vps29 belongs to the metallophosphatase (MPP) superfamily. MPPs are functionally diverse, but all share a conserved domain with an active site consisting of two metal ions (usually manganese, iron, or zinc) coordinated with octahedral geometry by a cage of histidine, aspartate, and asparagine residues. The MPP superfamily includes: Mre11/SbcD-like exonucleases, Dbr1-like RNA lariat debranching enzymes, YfcE-like phosphodiesterases, purple acid phosphatases (PAPs), YbbF-like UDP-2,3-diacylglucosamine hydrolases, and acid sphingomyelinases (ASMases). The conserved domain is a double beta-sheet sandwich with a di-metal active site made up of residues located at the C-terminal side of the sheets. This domain is thought to allow for productive metal coordination.
Statistics
?
PSSM-Id: 163637
View PSSM: cd07394
Aligned: 38 rows
Threshold Bit Score: 239.418
Threshold Setting Gi: 731636
Created: 18-Mar-2009
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
 
Conserved site includes 7 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1              # #                            #                           #              
1Z2W_A        12 LVLVLGDLHIPHRCNSLPAKFKKLLVpgKIQHILCTGNLCTKESYDYLKT-----LAGDVHIVRGDFDenl--------- 77  house mouse
2A22_A        27 LVLLIGDLKIPYGAKELPSNFRELLAtdKINYVLCTGNVCSQEYVEMLKN-----ITKNVYIVSGDLDsaifnpd----- 96  Cryptosporidium...
CAC34071       2 LVLVIGDFHVPHRSAAIPQVFLDRLNtgRIQTVLCTGNLCGKETYDILRT-----LAREVHVVKGDFDemq--------- 67  Entamoeba histo...
XP_764436     11 LLMVLGDLHVPQRSLFLPPCFKRLLKtdKIKRVICTGNVGSNEMLEVLND-----ISPSLHIVQGDYDddf--------- 76  Theileria parva...
CAJ08625       3 LVLVVGDTWVPQRASGVPEVFCKMFSpgRIHKLLITGGVGSKEMYDYLRT-----IAPEVHCVTSSVDrqwa-------- 69  Leishmania major
XP_829485      3 LVLVVGDLHVPQRAASIPKVFTQMFTpgRIQLVLITGNVGCREMYDYFRS-----IVPDVYCAKGEFDscwwpnvnskha 77  Trypanosoma bru...
XP_001348237  11 LVLLIGDFHSPIRNLGLPDCFKELLKtdKIKHVLCTGNVGCNENLELLKN-----IADSVHITKGDMDdnf--------- 76  Plasmodium falc...
XP_001583400   2 LILVIGDLHIPSRSYSIPAVFKESLStgKIHQILCTGNLCTRSEIEMLRK-----FCSDVQIVRGEFDedd--------- 67  Trichomonas vag...
XP_002294753   8 LVLLLGDHHIPSRSVSIPEPFQRMLIpnKMQHIVCTGNIGSVEEYQRLRElvggtAASNVHCVSGEYDsinssda----- 82  Thalassiosira p...
XP_001454897  12 LGLVIGDLNIPSRASDLPPQFKDLLVpnKVQYVFCTGNVGNRETTDWLKT-----LSGNTHFVKGDFDeak--------- 77  Paramecium tetr...
Feature 1                               #                            # #                         
1Z2W_A        78 -----nyPEQKVVTVGQFKIGLIHGHQVIPWgDMASLALLQRQFDVDILISGHTHKFEAFEHENKFYINPGSATGAYnal 152 house mouse
2A22_A        97 pesngvfPEYVVVQIGEFKIGLMHGNQVLPWdDPGSLEQWQRRLDCDILVTGHTHKLRVFEKNGKLFLNPGTATGAFsal 176 Cryptosporidium...
CAC34071      68 -----glNETEVIKIGNFKIGLMHGHQVIPWgDREALAIYQRQLDVDILITGHTHKLETKEVGGKYFLNPGSATGAYspl 142 Entamoeba histo...
XP_764436     77 -----nhPETLTINVGDLKIGVINGYQIPTWnNKDLLLKVAVDMNVDILVYGHSHVSDISKHGGKIFVNPGSATGCYqpw 151 Theileria parva...
CAJ08625      70 ----dhmPESVVLTVEGLKLGLIHGHQVPVG-DKDSLAAVQRELDVDVLVSGSTHQSKYFEFDSHLFVNPGSLSGADtey 144 Leishmania major
XP_829485     78 sdtdkllQDTHVINVESLRIGLIHGHQAIPCgDRDMLAMLQRKLDVDVLVSGATHNNKVFEFGGHLFVNPGSITGAFttr 157 Trypanosoma bru...
XP_001348237  77 -----dfPEDITLCIGDFKISLIHGHQIIPWgDMNALLQWQKKYDSDIIISGHTHKNSIVQYEGKYFINPGSVTGAFqpw 151 Plasmodium falc...
XP_001583400  68 ----vteCEQLSVTVGSFKIGLVSSYTLIPSnDKARLAAKARELDADILAFGGGHQAGMYQKDGKLYINPGSATGAFcae 143 Trichomonas vag...
XP_002294753  83 tqhnatsVKTKVIQLGSFRVGIIGGHQVVPWgDMSALSMVRRRLNVDVLICGWRRKNGVVEHEGGYYIFPGSITGAYssh 162 Thalassiosira p...
XP_001454897  78 -----diPETKIIQIGAWKLALVHGHQIIPAgDDESLYTFLKEMEADVLITGYTGVAKVSAVEKKYIINPGSVTGGFng- 151 Paramecium tetr...
Feature 1                                              
1Z2W_A       153 etNIIPSFVLMDIQASTVVTYVYQLi-GDDVKVERIEY 189 house mouse
2A22_A       177 tpDAPPSFMLMALQGNKVVLYVYDLr-DGKTNVAMSEF 213 Cryptosporidium parvum
CAC34071     143 vdNPVPSFMLLEINDSELTIYEYTLv-DGSVKCERVDF 179 Entamoeba histolytica
XP_764436    152 qpKSIPTFMLMAIQGSKVVIYVYEEh-DGEAQVIMTEL 188 Theileria parva strain Muguga
CAJ08625     145 dvNVVPSFMLLDIQDKSVVTFIYQFh-PEDETGGGGTA 181 Leishmania major
XP_829485    158 rlDVVPTFVLLDIQDKKVTSFSYAY--APGEGVGGEDF 193 Trypanosoma brucei TREU927
XP_001348237 152 lsEPTPTFILMAVAKSNIVLYVYEEk-NGKTNVEMSEL 188 Plasmodium falciparum 3D7
XP_001583400 144 npEPRPSFILINIQGNSAITYIYTLeaDGTMKVDKDVF 181 Trichomonas vaginalis G3
XP_002294753 163 taDVHPSFILLAVQGNKVVCYVYELi-NGEVDVSKTEF 199 Thalassiosira pseudonana CCMP1335
XP_001454897 152 qqSSIPSFLILEFKKEKIQVFIYTL--DGDVKIDKQEL 187 Paramecium tetraurelia strain d4-2

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap