2FBL,3BHD,2FJT,1D8H,3G3T,1D8H,2GFG,1YEM,3G3U,3G3R,3BHD


Conserved Protein Domain Family
CYTH-like_Pase

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cd07374: CYTH-like_Pase 
Click on image for an interactive view with Cn3D
CYTH-like (also known as triphosphate tunnel metalloenzyme (TTM)-like) Phosphatases
CYTH-like superfamily enzymes hydrolyze triphosphate-containing substrates and require metal cations as cofactors. They have a unique active site located at the center of an eight-stranded antiparallel beta barrel tunnel (the triphosphate tunnel). The name CYTH originated from the gene designation for bacterial class IV adenylyl cyclases (CyaB), and from thiamine triphosphatase. Class IV adenylate cyclases catalyze the conversion of ATP to 3',5'-cyclic AMP (cAMP) and PPi. Thiamine triphosphatase is a soluble cytosolic enzyme which converts thiamine triphosphate to thiamine diphosphate. This domain superfamily also contains RNA triphosphatases, membrane-associated polyphosphate polymerases, tripolyphosphatases, nucleoside triphosphatases, nucleoside tetraphosphatases and other proteins with unknown functions.
Statistics
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PSSM-Id: 143620
View PSSM: cd07374
Aligned: 38 rows
Threshold Bit Score: 62.0855
Threshold Setting Gi: 85544657
Created: 4-Oct-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 14 residues -Click on image for an interactive view with Cn3D
Feature 1:putative active site
Evidence:
  • Comment:This active site includes the putative metal binding and triphosphate binding sites.
  • Structure:3BHD_A; Human ThTPase bound to a citric acid molecule and a sulfate ion, contacts determined at 4A.
    View structure with Cn3D
  • Comment:For 3BHD_A, the citrate is positioned similarly to the triphosphate of ThTP, the sulfate ion is positioned similarly to the alpha-phosphate of the substrate.
  • Structure:1D8H_A; Saccharomyces cerevisiae mRNA-RTPase monomer bound with Mn2+; contacts determined at 4.5A.
    View structure with Cn3D
  • Structure:3G3R_A; Saccharomyces cerevisiae PolyP Polymerase bound with Mn2+; contacts determined at 4.5A.
    View structure with Cn3D
  • Structure:3G3R_A; Saccharomyces cerevisiae VTC4 polyP polymerase bound with a substrate analog (adenosine-5prime-[(beta,gamma)-imido] triphosphate) and Mn2+, contacts determined at 4.5A.
    View structure with Cn3D
  • Comment:Some of these residues were implicated from mutation analysis of Clostridium thermocellum TTM.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1        # # #                                        #                      # # #       
2FBL_A         5 EIERKFLvatfpd---------------------gelhaVPLRQGYLTtpt------------dsIELRLRQQgt----- 46  Nitrosomonas eu...
3BHD_A        26 EVERKFLpgpgteerlq-------------elggtleyrVTFRDTYYDtpels-------lxqadHWLRRREDs------ 79  human
3G3T_A        11 RQTTKYWvhpdnitelkliilkhlpvlvfntnkeferedSAITSIYFDnenldlyygrlrkdegaEAHRLRWYggms--- 87  baker's yeast
3G3U_A        11 RQTTKYWvhpdnitelkliilkhlpvlvfntnkeferedSAITSIYFDnenldlyygrlrkdegaEAHRLRWYggms--- 87  baker's yeast
3BHD_B        26 EVERKFLpgpgteerlq-------------elggtleyrVTFRDTYYDtpels-------lxqadHWLRRREDs------ 79  human
ACG39388       2 EVEIKLRlpdaaahrrls-----------aflaprlrrtHAQRNLFFDdaart-------lgaatAALRVRLYdgpddr- 62  maize
CAN72544       2 EVEVKLRlpdaashqkls-----------dllapfhvktLIQENIFFDgaaae-------lsskfAVLRLRFYdld---- 59  wine grape
XP_001778565   2 EVEVKIRlddalahqkva-----------allasnhqvtHLQENVFFDgaqqe-------lsskrAVLRLRFYngd---- 59  Physcomitrella ...
Q9SIY3         2 EVEVKLRlltaaahlrlt-----------tlltpyhlktLHQRNTFFDtpknd-------lslrrAVLRLRFLqnaavsa 63  thale cress
XP_001697877   2 EVEIKIRlpdraayeqvaaa------laaapggkgrldsHAQANYFFDgpnqe-------lnsrrVVLRVRTYdvd---- 64  Chlamydomonas r...
Feature 1                    #               #                                                   
2FBL_A        47 ------eyFMTLKSEgg-------lsRQEYEiqidvtqf----------------------------------------- 72  Nitrosomonas eu...
3BHD_A        80 --------GWELKCPgaagvlgphteYKELTaeptivaqlckvlradg-----------------------lgagdvaav 128 human
3G3T_A        88 ----tdtiFVERKTHredw---tgekSVKARfalkerhvndflkgkytvdqvfakmrkegkkpmneienlealaseiqyv 160 baker's yeast
3G3U_A        88 ----tdtiFVERKTHredw---tgekSVKARfalkerhvndflkgkytvdqvfakmrkegkkpmneienlealaseiqyv 160 baker's yeast
3BHD_B        80 --------GWELKCPgaagvlgphteYKELTaeptivaqlckvlradg-----------------------lgagdvaav 128 human
ACG39388      63 ---apsraVLALKRRariea--gvsrVEEIEeplepalavactddparlggld-------------spiirlvaaeygvg 124 maize
CAN72544      60 -----srcVLSLKSQpqisn--gisrAKEVEeeiepligracvaepwrfksm---------------ssriikrlrdefe 117 wine grape
XP_001778565  60 -----qrcVVTLKGKaviid--gisrGSEVEedidvalaracvadpsqfig-----------------sdckliqqiire 115 Physcomitrella ...
Q9SIY3        64 aspspprcIVSLKAKptlan--gisrVEEDEeeieywigkecvespaklsdig--------------srvlkrvkeeygf 127 thale cress
XP_001697877  65 -----kkaTVTLKGKqilen--gigrASEVEaevppaaaaayltqpsrmla------------------evpivkdaaek 119 Chlamydomonas r...
Feature 1                     # #                     #                                          
2FBL_A        73 emlwpateGRRVEKTRYSGKlp--------dgQLFELDVFAgh------------------------------------l 108 Nitrosomonas eu...
3BHD_A       129 lgplglqeVASFVTKRSAWKlvllgadeeepqLRVDLDTADf-------------------------------------- 170 human
3G3T_A       161 mlkkklrpVVRSFYNRTAFQlpg------darVRISLDTELtmvrednfdgvdrthknwrrtdigvdwpfkqlddkdicr 234 baker's yeast
3G3U_A       161 mlkkklrpVVRSFYNRTAFQlpg------darVRISLDTELtmvrednfdgvdrthknwrrtdigvdwpfkqlddkdicr 234 baker's yeast
3BHD_B       129 lgplglqeVASFVTKRSAWKlvllgadeeepqLRVDLDTADf-------------------------------------- 170 human
ACG39388     125 gdaapflcLGGFGNTRAVYEyeledg-gggvvLELDETRFDf-------------------------------------- 165 maize
CAN72544     118 vgdeglvcLGGFRNVRAVYAwng-------lnLELDETLFDf-------------------------------------- 152 wine grape
XP_001778565 116 ykceiflcLGGFRNVRNVYDweg-------vkLELDETHYAf-------------------------------------- 150 Physcomitrella ...
Q9SIY3       128 ndflgfvcLGGFENVRNVYEwrg-------vkLEVDETKYDf-------------------------------------- 162 thale cress
XP_001697877 120 fgvgslvaLGGFQNQRDVYEweg-------htLELDETKFEh-------------------------------------- 154 Chlamydomonas r...
Feature 1              # #                     
2FBL_A       109 sPLMLVEVEFlse---------daAQAFIP 129 Nitrosomonas europaea
3BHD_A       171 -GYAVGEVEAlvheeaevptalekIHRLSS 199 human
3G3T_A       235 fPYAVLEVKLqtqlgq---eppewVRELVG 261 baker's yeast
3G3U_A       235 fPYAVLEVKLqtqlgq---eppewVRELVG 261 baker's yeast
3BHD_B       171 -GYAVGEVEAlvheeaevptalekIHRLSS 199 human
ACG39388     166 gTRYELECETaepdr----vkavlERLLTV 191 maize
CAN72544     153 gTNYEIECESseper----akkllEGFLEE 178 wine grape
XP_001778565 151 gTTYEIECETndper----frqllGELLDK 176 Physcomitrella patens subsp. patens
Q9SIY3       163 gNCYEIECETeeper----vktmiEEFLTE 188 thale cress
XP_001697877 155 gTLYEIEVETeqpev----lrdrlEHWLSG 180 Chlamydomonas reinhardtii

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