Conserved Protein Domain Family
3MGA_Dioxygenase

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cd07366: 3MGA_Dioxygenase 
Subunit B of the Class III Extradiol ring-cleavage dioxygenase, 3-O-Methylgallate Dioxygenase, which catalyzes the oxidization and subsequent ring-opening of 3-O-Methylgallate.
3-O-Methylgallate Dioxygenase catalyzes the oxidization and subsequent ring-opening of 3-O-Methylgallate (3MGA) between carbons 2 and 3. 3-O-Methylgallate Dioxygenase is a key enzyme in the syringate degradation pathway, in which the syringate is first converted to 3-O-Methylgallate by O-demethylase. This enzyme is a member of the class III extradiol dioxygenase family, a group of enzymes which uses a non-heme Fe(II) to cleave aromatic rings between a hydroxylated carbon and an adjacent non-hydroxylated carbon. LigAB-like enzymes are usually composed of two subunits, designated A and B, which form a tetramer composed of two copies of each subunit. This model represents the catalytic subunit, B.
Statistics
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PSSM-Id: 153378
View PSSM: cd07366
Aligned: 10 rows
Threshold Bit Score: 389.828
Threshold Setting Gi: 90787428
Created: 20-Apr-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
Feature 1:putative Fe(II) binding site [ion binding site]
Evidence:
  • Comment:based on similarity to other members of the same protein family

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                  #                                                                     
BAC79261       2 AEIVLGIGTSHGPMLVTQTEQWrsrl---afdqSVNHAWRGGSWSYDQLVAEradqnfaaqitPEAMTAHNARCQASLDq 78  Sphingomonas pa...
YP_298976      2 ARLVAAFGSSHSIMLVCQREDWqhgfrqvdpknPHYFDRAGNPTTYEALLAIapdda-esmvtPERMGERYDQAEAAMDq 80  Ralstonia eutro...
ZP_03265562    2 AKIVSGVATSHTPLLTLGAAQWihrak-vdyenPELNTSDGRLMTYPQLLAEvgpcy-enevdVPTLAQKEQQCNDALDk 79  Burkholderia sp...
YP_001263552   2 AEIVLGIWTTHGPTLSTTPEQWllrlp--adraRGNHHFRGATYDFDALVALrdderlaeqasLVERTRRAEACQAAIRt 79  Sphingomonas wi...
YP_299962      2 AEIVLGMGTSHGPMLVTPPHTWgsrl---pddrRSLHHYQGRTWTFDELVQHrasenlaaqitPEVWEARHTRCMQAIEr 78  Ralstonia eutro...
ABD97974       2 GEIVAGYASSHAFTFIPPPRWEgfri----knrQSYTLRRGKTPPRLRKDEEp----------FDDAAARYAHIENGLQr 67  Mycobacterium s...
ZP_02885051    2 AKIVFGMAVPHSGMLGQAPEDWlknge--rdrsNPELWYRNRTWTYAELEAHreaaf-ekfltLEERTARAARCRAALDv 78  Burkholderia gr...
BAA31862       2 AEIVLGMWTSHGPTLSTTPEQWtlrv---sadlKRKHPFRGEEYSFEELVSCa----------AARSWRRPVPFPSASDa 68  Sphingomonas pa...
YP_003060980   2 AEIVFGMAQPHSGMLGQPPEDWltnge-rdrnnKKMLWYRNKRWDYEDLEAErgpenfepfltLEERTQRQARNHAAIQk 80  Hirschia baltic...
ZP_05482306    2 TEIVAGIGTSHGPQLKAPPPHAwdtrg-tadraSKALKFRGETYTYEELRELredfs--neiaPEVMARRWESCQRSMDr 78  Streptomyces sp...
Feature 1                                    #                                                   
BAC79261      79 ---------LAEIFSEAKIDVAVILGNDQMEIFDErLVPAFSVFYGDTITNYEFppermaalppginlsvagyipSGGAE 149 Sphingomonas pa...
YP_298976     81 ---------LRDRIRAARLDVLLVVGDDQTELFRTtNNPAFAIYYGDTIRNAKReespsdgwyk--karmarqepDADRD 149 Ralstonia eutro...
ZP_03265562   80 ---------LAAFVAQVKPDVAIIIGDDQAELFNSaNQPAFAIYHGQTAYTIDKlqepttpdwl--rtvrkgymlDQVHE 148 Burkholderia sp...
YP_001263552  80 ---------LADIYERARIDVAVIFGNDQHELFTEaLMPAFTIFNGPTIWNEPAsaeqarkmapgiheaeaghnpEVYTE 150 Sphingomonas wi...
YP_299962     79 ---------MSRVLAEARPDVAVIVGNDQMEIFDDmLVPAFSVMWGETIVNNMIsdeklsrlpp-gvahampgymPPEAT 148 Ralstonia eutro...
ABD97974      68 ---------LRDSIRQDRLDCLIIIGDDQNENFDGsALPQIAIHTGDGFTVSDRf-------------------lPEARF 119 Mycobacterium s...
ZP_02885051   79 ---------MAAAYRDANIDVAIILGKDQKEIFTH-CSPSIAIYSGEQVHNGPPqrsv--------------yapDHHVT 134 Burkholderia gr...
BAA31862      69 rpgakvpspTWPTFAAAKIDVAVIMGNDQRELFLEdVTPAITVYLGETIWDQPAtpeqaarmppgiheaewgiarPSAGT 148 Sphingomonas pa...
YP_003060980  81 ---------MKEAYEAANVDVAIILGKDQQEIFTH-MSPSIAIYSGEEVHNGPPqrpv--------------fapDKPVT 136 Hirschia baltic...
ZP_05482306   79 ---------LADFLRAQEVDVLVIVSSDHKETYGDeWLAPFSVFWGDEVAHVPFtraqldamapglaeaaagdvpDRTIM 149 Streptomyces sp...
Feature 1                                                                                        
BAC79261     150 YAGQPELARSIIAQAMADE----FDVAAMKALPkp----------etPHAFGFVYRRIMRDn-PVPSVPVLVNTfYPPNQ 214 Sphingomonas pa...
YP_298976    150 YPVNADMGRWMIRELCDRD----FDIAAMDGLErgq---------feGHAFSFIHRRYLQEl-DLPVVPVIVNTfDPPNQ 215 Ralstonia eutro...
ZP_03265562  149 LPCDPDMALTLIKGLIDEE----VDVATSDHVEdpln-------kgfGHAYGFVVKRLFAGl-QIPIVPVLLNTyFSPNV 216 Burkholderia sp...
YP_001263552 151 YPGAPELADHIIRQAVSDQ----FDVTRCARLPeqpgh----wnsgiGHAFGFVYRRIMRDr-VAPNVPIVTNTfFPPNQ 221 Sphingomonas wi...
YP_299962    149 YAGVPALGRHLIERTVADG----FDITALKHLSat----------etPHAFGFVFRQLMKDq-VIPTVPVLINTfYPPNQ 213 Ralstonia eutro...
ABD97974     120 WKSSPELSKDLSEHTVEAG----FDVATVVDFRett---------lhSHAHGEIVANILGDh-QIPVVLVFLNA-VHVPS 184 Mycobacterium s...
ZP_02885051  135 HQCHPKLASYLIGHFQREG----FDLTDLFAWPdnvwmkplpaypvvPHAYSFVYHQIMGDe-PPPHVPIIMNCfYPPTQ 209 Burkholderia gr...
BAA31862     149 IPASPSSACMCAKRWCRRLrsggLQDTARARRPlve---------rrAHSLGFIYRQIIRDq-VVPNLPIIINTfFPPNQ 218 Sphingomonas pa...
YP_003060980 137 HNCHPELANYLIKNFEKDG----FDITDLFSWPdnvwmk--paapivPHAFSFIYHQIMSDn-PPPHVPILMNCfYEPTQ 209 Hirschia baltic...
ZP_05482306  150 RPTHRALGKHIIQSTQADD----FDTGATEVIPagrye-----nhtiPHGFGFIYQRFLGQesTLPMVPIFINTfWEPNP 220 Streptomyces sp...
Feature 1                                                                                        
BAC79261     215 PTVRRCYEFGKSVLRGIQAWe-sDARVAVLASGGLTHF----------------VIDEEIDRLFFQAMEDRDIaRLADLG 277 Sphingomonas pa...
YP_298976    216 PTPRRCIQLGAALRELIAAYp-eDLRVGVLASGGLSHF----------------VVDAELDNAIIDAIRRKDTaFLASLD 278 Ralstonia eutro...
ZP_03265562  217 PSSRRCYNVGRAIRRVVEAIp-gDQRVMVIASGGLSHF----------------VVDEPLDRQIIAAFENKDAdFLQSIP 279 Burkholderia sp...
YP_001263552 222 PSARRCFELGRSVARAIRSWp-gDARVAVFGSGGMSHF----------------VIDEAFDRHLFDALQARDAeALCTIG 284 Sphingomonas wi...
YP_299962    214 PTVGRCHAFGRSLARAIRSWd-sDARVAIIASGGLTHF----------------VIDETVDQLILDSMRAHDFaPVEGLG 276 Ralstonia eutro...
ABD97974     185 LSPKRCFALGRAIADAVRSRrpaDERIGLYASGGLSHFtagypwaaydgprvhgSIDDEFDRRTLKCLATGNGyELSKLT 264 Mycobacterium s...
ZP_02885051  210 PSMSRCIEFGAVLRDAIDAWp-eDVRVGIFASGGLSHF----------------VNDEEFDRRIMKMLADYDYeGLAAVD 272 Burkholderia gr...
BAA31862     219 PTARRCFELGRAVGKAIRSWk-eDLPVAVFGSGGMSHF----------------VIDEDFDRMFFEALRNRDAeTLCAIE 281 Sphingomonas pa...
YP_003060980 210 PPMWRCIDFGKSLVKAIKDWd-sDARVAIIASGGLSHF----------------VCDEEFDRKIIEMLGNYDFeGLSALP 272 Hirschia baltic...
ZP_05482306  221 PGAKRCYDFGRAVGRAIRSFp-gDLRVGVVASGGLSHF----------------VIDEKLDQEFLQALRDQDAgYLSSVP 283 Streptomyces sp...
Feature 1                  #                                           
BAC79261     278 EAi-FQDGTSELKNWIPLAGMMAELGLdhEILDYVPCyrseAGTGNAMGFVCWR 330 Sphingomonas paucimobilis
YP_298976    279 PRq-LQAGSSEIRNWLIAVEAVRDLDL--EWTSYVPGyrtpAMTGTGLCFAAWR 329 Ralstonia eutropha JMP134
ZP_03265562  280 RGa-LNSGSSEILNWVTAAGALEHLNV--QSAEYFPLrrtpAGTGVGAGFVTWA 330 Burkholderia sp. H160
YP_001263552 285 EEh-LQSGTSELKNWIAAAGCLFDTPLsgGIVDYQPCyrseAGTGTANGFMAWT 337 Sphingomonas wittichii RW1
YP_299962    277 EAi-FQAGTSEVKNWIPVAGAMDELGFgmEVIDYVPCyrseAGTGNAMGFACWR 329 Ralstonia eutropha JMP134
ABD97974     265 SEdlLNSGNIELRSWICAVGAVGGNTP--WASVYEPIp--rALMGMAVAWTNCE 314 Mycobacterium sp. CH-1
ZP_02885051  273 NRs-YQSGTSEIKLYVSVMKAVQETRAsmTLVDYVPCwrtpAGTGEGMGFMYWK 325 Burkholderia graminis C4D1M
BAA31862     282 DKh-LQSGTSELKTWIAAAGALFDTDLkgDVVGYEPCyrseAGTGTANGFVAWQ 334 Sphingomonas paucimobilis
YP_003060980 273 ETs-YQSGTSEIKLYTSVMMALKDAGAkmTLVDYVPSyrtpAGTGEGMGFMYWS 325 Hirschia baltica ATCC 49814
ZP_05482306  284 AAe-MRSGASELRNWIAVAGIADEAGLavTSVDYEPCyrteAGTGNAMGFLTWE 336 Streptomyces sp. AA4

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