2PW6


Conserved Protein Domain Family
45_DOPA_Dioxygenase

?
cd07363: 45_DOPA_Dioxygenase 
Click on image for an interactive view with Cn3D
The Class III extradiol dioxygenase, 4,5-DOPA Dioxygenase, catalyzes the incorporation of both atoms of molecular oxygen into 4,5-dihydroxy-phenylalanine.
This subfamily is composed of plant 4,5-DOPA Dioxygenase, the uncharacterized Escherichia coli protein Jw3007, and similar proteins. 4,5-DOPA Dioxygenase catalyzes the incorporation of both atoms of molecular oxygen into 4,5-dihydroxy-phenylalanine (4,5-DOPA). The reaction results in the opening of the cyclic ring between carbons 4 and 5 and producing an unstable seco-DOPA that rearranges to betalamic acid. 4,5-DOPA Dioxygenase is a key enzyme in the biosynthetic pathway of the plant pigment betalain. Homologs of DODA are present not only in betalain-producing plants but also in bacteria and archaea. This enzyme is a member of the class III extradiol dioxygenase family, a group of enzymes which use a non-heme Fe(II) to cleave aromatic rings between a hydroxylated carbon and an adjacent non-hydroxylated carbon.
Statistics
?
PSSM-Id: 153375
View PSSM: cd07363
Aligned: 206 rows
Threshold Bit Score: 116.472
Threshold Setting Gi: 124515331
Created: 10-Apr-2009
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
 
metal bindingputative active
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1:metal binding site [ion binding site]
Evidence:
  • Structure:2PW6_A; Escherichia coli Uncharacterized Protein Jw3007 coordinates Zn.
    View structure with Cn3D

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1              #                                              #                          
2PW6_A        16 PALFLGHGSPXNVLEDNlyt-------rsWQKLGXTLp-----RPQAIVVVSAHWFTr--gTGVTAXETP-PTIHDFGg- 79  Escherichia coli
XP_001018757  10 PSVFISHGLVGETLRPGtdl------feqLNKFSKSQsy--lpNVETVIIVSGHWEC----DEITVNLNP-QPELDHDh- 75  Tetrahymena the...
NP_376674     11 KGIFVSHGSPMILVENDpwk-------dlLREWGKRLg-----KFDSVIIISPHFFSwsgtFLVETQPKL-ECIQDYYg- 76  Sulfolobus toko...
YP_001190550   2 IGLFVSHGSPTILVEDIpwk-------klMREVGEKIrs--evNPETVVVISPHFISwsgkHLVEVQEKL-ECIQDYYg- 70  Metallosphaera ...
XP_001015177   9 PSLFISHGDVGQTLNPEtql------ykcLDKYAKDNkl--rsRIKTAIIISGHWEEk--qVTINLNPHP-KLVHDHPg- 76  Tetrahymena the...
XP_001390106   7 PTYFIGHGGVSILFKPGygpv-----qqdLREIGKEIka---lKPEAIVITCGHFQGe--gDAIEVNLKE-PTKVWHDl- 74  Aspergillus nig...
NP_343447      8 IGLFISHGSPTILIDENkwk-------dlLRRVGKEIee--kyKPETIIVSSPHFVSwtetFYIESSEKL-ECIQDYYd- 76  Sulfolobus solf...
YP_256820      4 VGYFISHGSPMILVEENpwk-------ghLADLGKEIkn--kyDPETVIISSPHFFSwgegNYTVISKDL-KCIQDYFg- 72  Sulfolobus acid...
NP_394576      8 MTLNAMVYAPNTPTLIGdlgvkhteteraLNEIGRSCa----dRIDTVIVVSPHFITsg-gFGIVASERP-KQLYDFYg- 80  Thermoplasma ac...
EES51550       3 RIWPVMAPHSPDLLSSWsrgdrr-emtaaFVRFRQEIftlsgePFDAVIAYTPAWQV----RHVTLMDLSsEHRSSRDfs 77  Leptospirillum ...
Feature 1                                                                                        
2PW6_A        80 FPQALYDthYPAPGSPALAQRLVELlapIPVTLDkea-wgFDHGSWGVLIKXYPDa-------------dIPXVQLSIDs 145 Escherichia coli
XP_001018757  76 PSEWLYKfdFRLETNVPLANKIKDKlkkSGMTIKttttasADHGAWGGLFALYDIntqqkvyhsqnkqyvPKVVLVSLDt 155 Tetrahymena the...
NP_376674     77 FPDELYKfcYSADNDVDLANKIVEEgqkEGLPIKqdnkwgLDHGAWIPLMYMYPEg--------------VKAVTISITd 142 Sulfolobus toko...
YP_001190550  71 FPDELYKfcYSADNDVELARQIVSAgkkAGLEVEedsswgLDHGAWIPLSYMFPG---------------LRVVSISITd 135 Metallosphaera ...
XP_001015177  77 RWLYKYN--FSLDTNVELAKQIHQKlldNKIQAKtttsedIDHGGWGGLFCLYDIgeqqkaf-qsntkyaPQIVQVSLHs 153 Tetrahymena the...
XP_001390106  75 GPSWHTT--FPHVFEYQYAHKSSRA---LGEKTSg-----LIETRWVPFKLMFPEged---------eldIPILQISTYa 135 Aspergillus nig...
NP_343447     77 FPDETYKycYNALNDTELVEEIVKNa--EGKVVKddk-wgLDHGAWIPLFYMFPEy-------------kPKVVTISITd 140 Sulfolobus solf...
YP_256820     73 FPKELYEfcYQAENDVELASELIKEs-nGMLKVEek--wgLDHGAWIPLYYMFPEd-------------kPKVVPISISs 136 Sulfolobus acid...
NP_394576     81 FPDSFYLvkYDPPGNPEMGEMLIREllkQGIPAGnatswgLDHGAWSPLRLMFPDa-------------dKRVLPVSISp 147 Thermoplasma ac...
EES51550      78 GFGHHYQ--YDPPGSPELGERLMAAgraKGLAMAsgv-hgVDHAISIPLFFLLPEg-------------tTPVLPVSQSl 141 Leptospirillum ...
Feature 1                                                                                        
2PW6_A       146 skpAAWHFEXGRKLAALRDe---GIXLVASGNVVHNLRTVkwhgdsspyPWATSFNEYVKANLtwqgpveq-hplvnyld 221 Escherichia coli
XP_001018757 156 kmdPQKHYDLGKILGEFRDe---KTMIICSGASTHNFNYNrkp---gftEQSKIWEKWLQSIVtdqnveir-hsivnfkq 228 Tetrahymena the...
NP_376674    143 l-sAEIHYKLGEVIGRVVGd--rNVLIIGTGSPTHRLELMyln----akPRQTKFDQILIQKLkdgdfts----lfkleg 211 Sulfolobus toko...
YP_001190550 136 g-tPQEHYKLGEVIAQASSg--rRVLILGTGSPTHRLDGLylg----ikPKQSRFDQVLMGLLkegkfde----imelpk 204 Metallosphaera ...
XP_001015177 154 gmdTAFHYKIGQILEQFRDe---NTIIINSGQSTHSFTYWhhp---qftEWSKEWEKCLLQIItdkdekkrkdaiiaikq 227 Tetrahymena the...
XP_001390106 136 gndLNAHVRLGEALSSLPG----RILLIGSGMIVHNLRATregt--tdtTVARSIEDAALKALsaptpserdaqflafqe 209 Aspergillus nig...
NP_343447    141 n-sPESHYEIGEKIRKAVEklgrKAVFLATGSPTHRLDLFyfk----itPKPTKFDAILMDLIksgefeki--lkisely 213 Sulfolobus solf...
YP_256820    137 a-sPRRHYELGSIIRKVSEklgrRTLFIATGSPTHRLDLLyfn----lkPRATKFDVLLIKLInegkfedi--ldmdsky 209 Sulfolobus acid...
NP_394576    148 algADSHLKLGMAIRKVSSe--hDAMLLVTGSIIHRLDLYskgt-gempEEALKYLELAAKYMvagswe-----aiwnis 219 Thermoplasma ac...
EES51550     142 s-gVLHAHRLGEAIRALGPkrreKILLVVSGVLSFNAMEMarg---hedPAIPRWTEKIIESLvgsdlpd---wsllpsl 214 Leptospirillum ...
Feature 1                      #                                     
2PW6_A       222 HEGGTLSNPTPe--HYLPLLYVLGAWDgqepitIPVEGIEXg-SLSXLSVQI 270 Escherichia coli
XP_001018757 229 NKLYHYMHHSDd--HFMPLVVASGSSRep--gqLICLELQF--TSMCSCYLF 274 Tetrahymena thermophila
NP_376674    212 TKEWEIASPEG---MLRPLFVVLGAVKpkk-aeIIGYEVPYg-GVSMLAVEF 258 Sulfolobus tokodaii str. 7
YP_001190550 205 TKMWVEAQPEG---LLNPLYTVLGFVKprk-aeILGYDVPWg-GVSMLAVRF 251 Metallosphaera sedula DSM 5348
XP_001015177 228 HKLYNYMHHSDd--HFMPLLVSLGTSQyp--gnLICLEQQH--TFMSSCYQF 273 Tetrahymena thermophila
XP_001390106 210 REDWIDAHPTEe--HVLPLYMTLGVGRnws-eyKVWNQDRYivGQSYLSFRL 258 Aspergillus niger CBS 513.88
NP_343447    214 PKEYQTAMPEG---NLNTLHMLLGYIKpkk-aeILGYDTPWp-GVSMLAASF 260 Sulfolobus solfataricus P2
YP_256820    210 PKEYSSATPEG---DLKTLHTLLGFVRptk-akVLGYDIPWa-GVSMLSALF 256 Sulfolobus acidocaldarius DSM 639
NP_394576    220 HDLFIAASPEG---GEYPSRVLAGSASnrmrgrIMAQELEFn-AVSLTTILF 267 Thermoplasma acidophilum DSM 1728
EES51550     215 GKDVLDVLDPPgrlRELHLLKGLGVSGg-----RLWAHERIe-GLVQTLISF 260 Leptospirillum ferrodiazotrophum

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap