Conserved Protein Domain Family
HPCD_like

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cd07362: HPCD_like 
Class III extradiol dioxygenases with similarity to homoprotocatechuate 2,3-dioxygenase, which catalyzes the key ring cleavage step in the metabolism of homoprotocatechuate.
This subfamily of class III extradiol dioxygenases consists of two types of proteins with known enzymatic activities; 3,4-dihydroxyphenylacetate (homoprotocatechuate) 2,3-dioxygenase (HPCD) and 2-amino-5-chlorophenol 1,6-dioxygenase. HPCD catalyzes the key ring cleavage step in the metabolism of homoprotocatechuate (hpca), a central intermediate in the bacterial degradation of aromatic compounds. The enzyme incorporates both atoms of molecular oxygen into hpca, resulting in aromatic ring-opening to yield the product alpha-hydroxy-delta-carboxymethyl cis-muconic semialdehyde. 2-amino-5-chlorophenol 1,6-dioxygenase catalyzes the oxidization and subsequent ring-opening of 2-amino-5-chlorophenol, which is an intermediate during p-chloronitrobenzene degradation. The enzyme is probably a heterotetramer composed of two alpha and two beta subunits. Alpha and beta subunits share significant sequence similarity and both belong to this family. Like all Class III extradiol dioxygenases, these enzymes use a non-heme Fe(II) to cleave aromatic rings between a hydroxylated carbon and an adjacent non-hydroxylated carbon.
Statistics
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PSSM-Id: 153374
View PSSM: cd07362
Aligned: 5 rows
Threshold Bit Score: 414.609
Threshold Setting Gi: 258511590
Created: 10-Apr-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
putative metalputative active
Feature 1:putative metal binding site [ion binding site]
Evidence:
  • Comment:based on similarity to other members of the same protein family

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1               #                                                #                       
BAG80151       5 ALAAKITHVPSMylselpgknhgcrqg----aidghkeiskrcremGVDTIIVFDTHWLVnsaYHINCADHFegv-ytSN 79  Escherichia col...
AAK26519       7 ISGFLAPHPPHMlyaenppqneprsnggweqlrwayerarasvealKPDVLLVHSPHWITsvgHHFIGVPELsg--rsVD 84  Pseudomonas putida
ZP_04392152    4 ELAMLVPHTPRMcfedrtpefqhelv-------kgmhevakiieqvKPDTLVLISCHWMSs-fDHFVDATPRhkgvltAV 75  Geobacillus sp....
BAH79099       4 EMALLAAHVPSIchesnvpdfqqdlv-------kglkqmrdrinelQTDVILLMSCHFPAt-fHHYVDATPRhtgiltAM 75  Paenibacillus s...
YP_003185024   4 ELAMITPHTPRIchrdrvpefqkpmv-------eamlkcadvidqiRPDVVVIISCHWMSs-fMHYVDVTPRhkgiltAV 75  Alicyclobacillu...
Feature 1                                                                                        
BAG80151      80 ELPHFIRDMTYNYEGNPELGQLIADEALKLGVRAKahnIPSLKLEYGTLVPMRYMNEDkhFKVVSISAFct--vHDFADS 157 Escherichia col...
AAK26519      85 PIFPNLFRFDYSMKVDVDLAEACYEEGRNVGLETKmmrNPRFRVDYGTITTLHMIRPQwdIPVVSISANntpyyLSMEEG 164 Pseudomonas putida
ZP_04392152   76 ECPDLIADVPYDYPGDEELGKQLVKAGKEAGLRVVevnDPTYIWDYGTVVPLRYLAPNedIAVISLSVTw---aANLEET 152 Geobacillus sp....
BAH79099      76 ECPDLISDVPYDYPGDEELARKLVTAGQEAGLPIVeinDPTYIWDYGTVVPLRYLVPNqdKSVISLSVCw---aSSLEES 152 Paenibacillus s...
YP_003185024  76 ECPDLISDVPYDHPGHPELGRRLVEAGQRNGLQVVavdDPTYVWDYGTVVPLRYLLKRp-TPVVALSVCw---aASLEES 151 Alicyclobacillu...
Feature 1                                                                                        
BAG80151     158 RKLGEAILKAIEQy----dGTVAVLASGSLSHRFiddqraee---gmnsytrEFDRQMDERVVKLWREGQFKEFCNMLPe 230 Escherichia col...
AAK26519     165 LTEMDLLGKATLEavrksgKRAVLLASNSLSHWHfhqepeppedmtkehpesLAGYQWDMRMIDLMRRGQMQEVFRLLPq 244 Pseudomonas putida
ZP_04392152  153 YTWGQVIGKVLREs----kKKTVFVCSGALAHNLvrrpe---------alptLAEQALDRQFLQYLQSNNVQAAWNMLPq 219 Geobacillus sp....
BAH79099     153 YQWGVQIGKVLREs----eKRAVFISSGALSHNLvrgrh---------hmpsRSEQAMDNQFIEYLLNGDYNAAREMLNq 219 Paenibacillus s...
YP_003185024 152 MRWGEVMGDVFLEs----pERVVFLASGALAHNLgrgpe---------kwpnLTEQALDREFCQYLVKGDKASAVSMLPs 218 Alicyclobacillu...
Feature 1                                                          
BAG80151     231 yady-cygeGNMHDTVMLLGMLGwdkydgKVEFITELFPSSGTGQVNAVF 279 Escherichia coli SE11
AAK26519     245 fieeafaevKSGAFTWMHAAMQYp----eLAAELHGYGTVIGTGNAVMEW 290 Pseudomonas putida
ZP_04392152  220 yaraagvesGGRHLAALLGVIQEn-----YQSRYYGYGQSSGSGNVVMTF 264 Geobacillus sp. Y412MC52
BAH79099     220 yariagvesGGRHLAALLGVLDDk-----QRAEFWGYGQSSGSGNAIISF 264 Paenibacillus sp. JJ-1b
YP_003185024 219 yvraagvesGGRHVAVLLGVLRKq-----FEGELHGYGPSSGSGNPVLTI 263 Alicyclobacillus acidocaldarius subsp. acidoc...

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