1BOU,1B4U


Conserved Protein Domain Family
PCA_45_Doxase_B_like

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cd07359: PCA_45_Doxase_B_like 
Click on image for an interactive view with Cn3D
Subunit B of the Class III Extradiol dioxygenase, Protocatechuate 4,5-dioxygenase, and simlar enzymes.
This subfamily of class III extradiol dioxygenases consists of a number of proteins with known enzymatic activities: Protocatechuate (PCA) 4,5-dioxygenase (LigAB), 2,3-dihydroxyphenylpropionate 1,2-dioxygenase (MhpB), 3-O-Methylgallate Dioxygenase, 2-aminophenol 1,6-dioxygenase, as well as proteins without any known enzymatic activity. These proteins play essential roles in the degradation of aromatic compounds by catalyzing the incorporation of both atoms of molecular oxygen into their preferred substrates. As members of the Class III extradiol dioxygenase family, the enzymes use a non-heme Fe(II) to cleave aromatic rings between a hydroxylated carbon and an adjacent non-hydroxylated carbon. LigAB-like class III enzymes are usually composed of two subunits, designated A and B, which form a tetramer composed of two copies of each subunit. This model represents the catalytic subunit, B.
Statistics
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PSSM-Id: 153372
View PSSM: cd07359
Aligned: 22 rows
Threshold Bit Score: 237.945
Threshold Setting Gi: 67154469
Created: 17-Apr-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1:Fe(II) binding site [ion binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                 #                                                                      
1BOU_B         3 RVTTGITSSHIPa-------------------------LGAAIQTGtsd----------------ndyWGPVFKGYQPIR 41  Sphingomonas pa...
ZP_00416214    1 MTIKLKCLSHTP--------------------------LRGLNDPGad-------------------vVAEVDAVLARSR 35  Azotobacter vin...
BAC79261       3 EIVLGIGTSHGPmlvtqteqw---------rsrlafdqSVNHAWRGgswsydqlvaeradqnfaaqitPEAMTAHNARCQ 73  Sphingomonas pa...
ZP_01012705    3 EITGIYTASHTPv-------------------------MLNFPDSIpa------------------alKDEIFAAYKAMG 39  Rhodobacterales...
ZP_05520124    3 DIVLGVGASHST--------------------------LMNTHWEEtfh----------------kdrAERFRDALREAR 40  Streptomyces hy...
ABC33900       2 GDFLALGMTHYPllagtdehmagllrwtltdpdipgeeKDPAHWPDrmrrewsdd----ggtaaaaahRAELVANLARVR 77  Rhodococcus sp....
Q8L178         3 RLVGAYATSHTAm-------------------------MIRKLQPDnd-------------------vHAAVHQAFAQVR 38  Rhodococcus sp....
YP_003203382   3 EIVMAYSASHAPm-------------------------MTADPGSApr------------------eqADSFFGALEKVR 39  Nakamurella mul...
ZP_04690630    3 QLVLGIGASHST--------------------------LMNTHWAEvdh----------------lpaAHRFRDGLAQAR 40  Streptomyces gh...
YP_923338      1 MDAIGIGMTHYPmlagtdehmadllkwtlndpgipagyKDPANWPPlmqkewadd----egvaaaaghRADLLEHLRRCR 76  Nocardioides sp...
Feature 1                                #                                                       
1BOU_B        42 DWIKqp-----gNMPDVVILVYNDHASAFDMnIIPTFAIGCAETFKPADEgw-----------------gpRPVPDVKGH 99  Sphingomonas pa...
ZP_00416214   36 AEVEa-------FDPELIVIFAPDHYNGLFYdLMPPFVIATAAESVADYG---------------------TLPGPLSIP 87  Azotobacter vin...
BAC79261      74 ASLDqlaeifseAKIDVAVILGNDQMEIFDErLVPAFSVFYGDTITNYEFppermaalppginlsvagyipSGGAEYAGQ 153 Sphingomonas pa...
ZP_01012705   40 DDFMa-------GKPDALIVLSNDHLHNFFLdNFPAYCIGCAESYESPIEhw-----------------lhATRRTFAGD 95  Rhodobacterales...
ZP_05520124   41 DEIAa-------ARPDTVILVGSNHFRGFWLdLIPSFTIGVGECVASGESg--------------------TPEGPQPVD 93  Streptomyces hy...
ABC33900      78 EAVDe-------FAPDVVVVWGDDQYENFREeVVPPFCVLAYGDLEVPAFevmnergs-----pnfwgkpdDFQIVMHGD 145 Rhodococcus sp....
Q8L178        39 AEIDr-------LSPDVLVVVSSEHLTSFSYdSFPQICVGIGEIATGWGDg-------------------gVASAEVPLA 92  Rhodococcus sp....
YP_003203382  40 DKCRe-------LDVQAIVMMTGEHFTNFFLdGLPQIAIGLDDEHLGPPEvw-----------------lqVPKKMVPSN 95  Nakamurella mul...
ZP_04690630   41 DALAa-------ARPDAVVVIGSNHFRGMFLdLMPAFTVGVGEVNGAGEAg--------------------TPGGPLPVD 93  Streptomyces gh...
YP_923338     77 DYLDd-------FQPDVVVVWGDDQYENFREeVVPPFCVLAYPDLEVAPFehmnefgv-----pnawgwpdDFTIPVRGD 144 Nocardioides sp...
Feature 1                                                                                        
1BOU_B       100 PDLAWHIAQSLILDEf-DMTIMNQMd----vDHGCTVPLSMIFGEpe--ewPCKVIPFPVNv------------------ 154 Sphingomonas pa...
ZP_00416214   88 RDLALDLTRHILDSGl-DIALSHRLq----vDHGCTQTLEELTGSl----tRYPVIPIIINs------------------ 140 Azotobacter vin...
BAC79261     154 PELARSIIAQAMADEf-DVAAMKALpkp-etPHAFGFVYRRIMRDn-----PVPSVPVLVNt------------------ 208 Sphingomonas pa...
ZP_01012705   96 QAFGSYLLKNALEAEf-DPSFSMEMv----lDHGSLTPLELAGVDp-----DLPIVPVLINc------------------ 147 Rhodobacterales...
ZP_05520124   94 VSLARHIANTLVEGGrfDLAYSAKLq----iDHGQSHAIQYLLDGl-----DVEIVPLVVNv------------------ 146 Streptomyces hy...
ABC33900     146 ASGARQLTDGLIERGf-DMAYSYKKragahfPHAILNTQLFLDYAnagaemSYPFIPMTVNcygqhaiarkgglarfaei 224 Rhodococcus sp....
Q8L178        93 GAFAAQLLSEGVAAGf-DLAFSANPk----iDHAFMAPLTLIRPEm-----DIPVVPVFQNa------------------ 144 Rhodococcus sp....
YP_003203382  96 AGLAAHITASLIEQDv-QPALSYGMk----iDHGFMTVYYWLDNSm-----ELPMVPIVLNc------------------ 147 Nakamurella mul...
ZP_04690630   94 TDLARGLVDGLVDDGf-DPAFSLRLt----vDHGITHSLQHLVPDl-----DVPVVPVVINm------------------ 145 Streptomyces gh...
YP_923338    145 AARARQLTNDLVRAGf-DVAYSYEKransrfPHAILNTQLFLDYDnagskfPYALVPMTVNcygqhaivrrggmarfadi 223 Nocardioides sp...
Feature 1                                                                                        
1BOU_B       155 ----VTYPPPSGKRCFALGDSIRAAVESfpedLNVHVWGTGGMSHqlqg------------------------------- 199 Sphingomonas pa...
ZP_00416214  141 ----VAPPFAPYRRIRKLGEAVGRFVASl--gKRVLILGTGGLSHeppvpllsgaaeeiaefliagrnptpearaarqar 214 Azotobacter vin...
BAC79261     209 ---fYPPNQPTVRRCYEFGKSVLRGIQAwesdARVAVLASGGLTHf---------------------------------- 251 Sphingomonas pa...
ZP_01012705  148 ----VQPPMPTMRRSYNLGKFLGQAIANydgvDRVAILATGGISHdiat------------------------------- 192 Rhodobacterales...
ZP_05520124  147 ----FAHPLPTLERCERLGRAIREAVLAfpegRRVAVIGSGGLSHrlpwpdwrdphgddeefmvgawl------ngrdhw 216 Streptomyces hy...
ABC33900     225 esevLDPSGPTPARCFQVGRAVAQHFRDh--dLRVAFVASSSWSHaflad------------------------------ 272 Rhodococcus sp....
Q8L178       145 ----NTEPLPPLWRSAQLGELLRDVITRrpaaETVVVLGTGGLSHwvgt------------------------------- 189 Rhodococcus sp....
YP_003203382 148 ----TTPPLMTIRQFHEFGKKLGKAIRSyeglERVALVAGGGLSHfvge------------------------------- 192 Nakamurella mul...
ZP_04690630  146 ----FAPPLPSLRRCHAFGAALRKAVHGdgrdKRIAVIASGGLSHrlpwpkwfaalsdddrflvrawl------ngrtsw 215 Streptomyces gh...
YP_923338    224 enedVDPVGPSPQRCFELGRAMARSFRDr--dLRVAFVASSSWSHaflnd------------------------------ 271 Nocardioides sp...
Feature 1                                                                #                       
1BOU_B       200 ------------pragLINKEFDLNFIDKLISDPeel-skmPHIQYLREs-GSEGVELVMWLIMRGALPEkv-------- 257 Sphingomonas pa...
ZP_00416214  215 tiaagkiygtaespltPLNTDWDLAFIDLLVQGRlaeiddfVVEEISTTa-GRSTHEIRTWVAAFAALAAgga------- 286 Azotobacter vin...
BAC79261     252 ----------------VIDEEIDRLFFQAMEDRDi-----aRLADLGEAifQDGTSELKNWIPLAGMMAElgl------- 303 Sphingomonas pa...
ZP_01012705  193 ------------prmgMVNEEFDQTFLAHMESGDvda-tiqYATDFVHTa-GNGCEEIRMWMAAMGAANGat-------- 250 Rhodobacterales...
ZP_05520124  217 kdydvkrreiiraaesAINEDFDEEFLSLVENGRthe-vtrYTTEELEKvaGNGAQEVRTWLLMAALLGHap-------- 287 Streptomyces hy...
ABC33900     273 -----------klwhlRPDTEADLRIYKALLEDDfa----lMREYTSTEvvDAGQHEILNWFCMLGAVEE---------- 327 Rhodococcus sp....
Q8L178       190 ------------pemgQINSAFDERFLQHVRAGDlgailamKTADVLAEa-GNGAPEICNWVTAMAAASSqegwgvsepe 256 Rhodococcus sp....
YP_003203382 193 ------------prigDIDEEFDNWFLDILAKGDltd-iidIPNDELMEa-GNGTGEIRAWVALAGAMNDap-------- 250 Nakamurella mul...
ZP_04690630  216 geyevrrrqiiraaapDINEAFDQWFLHLLETGDlselldrTDEQIEKEa-GNGAHELRAWIAMTAALTSpegl------ 288 Streptomyces gh...
YP_923338    272 -----------kswhiVPDTPADEKLYEAFQAEDwa----aFTAPTVEHivGAGQHEMLNWFCLAGACAE---------- 326 Nocardioides sp...
Feature 1                                          
1BOU_B       258 ------rdLYTFYHIPa------sNTALGAMILQ 279 Sphingomonas paucimobilis
ZP_00416214  287 -----yraRQDYYRPIne-----wIAGYGVVSAE 310 Azotobacter vinelandii
BAC79261     304 ------dhEILDYVPCyrse-agtGNAMGFVCWR 330 Sphingomonas paucimobilis
ZP_01012705  251 -------yERTYYKAVnd-----wYTGIGIGRFK 272 Rhodobacterales bacterium HTCC2654
ZP_05520124  288 -------gRRLSYESLpe-----wLTGMGVAVLD 309 Streptomyces hygroscopicus ATCC 53653
ABC33900     328 --------LGLRREWSdlvvtdvfNSNKAFAVYS 353 Rhodococcus sp. T104
Q8L178       257 rtargprgVVLAYESVpd-----wATGIALARLA 285 Rhodococcus sp. YK2
YP_003203382 251 -------gEVLSYVPVye-----wINGMGVVLYK 272 Nakamurella multipartita DSM 44233
ZP_04690630  289 -----ptgRTLAYGAVpd-----wLTGMGVSLLT 312 Streptomyces ghanaensis ATCC 14672
YP_923338    327 --------SGLELNWSdlvvtdlfNSNKCFALFS 352 Nocardioides sp. JS614

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