Conserved Protein Domain Family
PriL_PriS_Eukaryotic

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cd07322: PriL_PriS_Eukaryotic 
Eukaryotic core primase: Large subunit, PriL
Primases synthesize the RNA primers required for DNA replication. Primases are grouped into two classes, bacteria/bacteriophage and archaeal/eukaryotic. The proteins in the two classes differ in structure and the replication apparatus components. Archaeal/eukaryotic core primase is a heterodimeric enzyme consisting of a small catalytic subunit (PriS) and a large subunit (PriL). In eukaryotic organisms, a heterotetrameric enzyme formed by DNA polymerase alpha, the B subunit and two primase subunits has primase activity. Although the catalytic activity resides within PriS, the PriL subunit is essential for primase function as disruption of the PriL gene in yeast is lethal. PriL is composed of two structural domains. Several functions have been proposed for PriL such as stabilization of the PriS, involvement in synthesis initiation, improvement of primase processivity, determination of product size and transfer of the products to DNA polymerase alpha.
Statistics
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PSSM-Id: 143474
View PSSM: cd07322
Aligned: 48 rows
Threshold Bit Score: 306.496
Threshold Setting Gi: 215501499
Created: 3-Mar-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
PriL_PriS
Feature 1:PriL_PriS interface [polypeptide binding site]
Evidence:
  • Comment:Archaeal/eukaryotic primases form a heterodimer consisting of a small catalytic subunit (PriS) and a large subunit (PriL).

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                        
Q8NIZ4        40 FYSTPPTADITIEQ------FEQWAIDRLRILAELEACsfrnktpaetashmkpl------------------------- 88  Neurospora cras...
Q55BM5        22 LYINVPDFNCNMSQ------IENYCIDRFNLLNEVSKLkdtlsaqksntskdne-------------------------- 69  Dictyostelium d...
XP_822563     34 MYTCNPAGNKTLFE------LEAIVVKRLELLGWIDQLlnspnmknlsqlldevgahlplerrkqstqpaadhvvlgrds 107 Trypanosoma bru...
XP_001561600  34 MYEQKPHGNSTLFE------LEAMVAKRMELLAWIDQRmnspqaksfesvldtiiarlpeerrsstatdasrgkmvslgy 107 Leishmania braz...
XP_001023701  20 ILLENAIDNSNIQGfqtiqeIVQLAMQRMKMIKEIIQEeprfnlidplskpnynrirv---------------------- 77  Tetrahymena the...
XP_001300881  22 LYNVEPSANISISD------MIEFAYLRSQVHQEINDLengiekrdqfekimikygipik-------------------- 75  Trichomonas vag...
XP_001827849  15 FYDKLNIIMPGKIPye---tFKSYSIDRIKLLRKIESGtdn--------------------------------------- 52  Enterocytozoon ...
Q8SRV4        17 FYTALSKSSVDYET------FRKCAERRIEMLRCEEGDgiis-------------------------------------- 52  Encephalitozoon...
XP_001306374  22 TYETIPSMQLSFEE------ILSIAEKRFQFHRLLEFEesqetvefskirsl---------------------------- 67  Trichomonas vag...
Q517X5        56 VYQTVGTISWSNVYda---lEKRWSLYRLMYNEETNSEkitqels----------------------------------- 97  Entamoeba histo...
Feature 1                                                                                        
Q8NIZ4        89 --------------ldkylpldtnsssssqlFAQRQKDHYSHFILRLAFASt-------EDLRRRFTRVETMLFRMRLna 147 Neurospora cras...
Q55BM5        70 ----------------dfrnlssrflgstkdEQFKIKDELSHYCLRLAMCD--------STDRNWFITTESILLANRLn- 124 Dictyostelium d...
XP_822563    108 vdgstsdtpgsraprhssrsltamlstqnvfVFEGDEDLLSHRLARFVFCMs-------EKWRKWFVRTEEMLLRARLrl 180 Trypanosoma bru...
XP_001561600 108 detasgsgsgrrsssrgstggaagssqaasiVFAPEEDVTSHLLCRFAFCMs-------ERWRDWLVRTERVLLMARIkm 180 Leishmania braz...
XP_001023701  78 -----------ankfitvaipksdpeyelknKQQYAKEVLSHFLCKMAYAFya-----ePETWLEFARAEAFILMDKLk- 140 Tetrahymena the...
XP_001300881  76 ----------rprdgvfnpsnsvipnfecgqITKQFLDEMSFYAFMVISLRt-------DETQHKFVKVESEIFKARI-- 136 Trichomonas vag...
XP_001827849  53 ----------------------------iftIDSLYKDLISHFALRLIVIQs-------HVTLQWFVTMETKLFKYRInq 97  Enterocytozoon ...
Q8SRV4        53 ----------------------------kclSTSIEEDILSHFFCRVVCSQd-------AWNAVWFVNAEVNLLRLRIsk 97  Encephalitozoon...
XP_001306374  68 -----------------sleydiplkeasetDGEQTKDQISFFSLLMIYTEt-------EEQQTKLADYESRLFFYRLkl 123 Trichomonas vag...
Q517X5        98 -------------------------elvkvnENNRWDDEVSYALFFASFCDfqnnvrcnKKLENWFVLCEMRVYLARIet 152 Entamoeba histo...
Feature 1                                                                            ##  #   #   
Q8NIZ4       148 ------ddGRERAAFINSLNLDWETVsdeekrelaaelaatasfggykkgqqqqqqyeedqQTWCKVSWLRVPELVEQRr 221 Neurospora cras...
Q55BM5       125 -------qSDLTTIISSFKGFGWSPVsqeeyeqyqddlgy-------lvkkanlnlnevnnTHFYKLPFQDIPKLIDTKk 190 Dictyostelium d...
XP_822563    181 ql--akceADFLPNLMRLNGLPCDALtddqqqdprlqkyii------yhtavqgkgqlrraDEYFLVPLSLATRLIKARs 252 Trypanosoma bru...
XP_001561600 181 ev--aknpLTFLVDLMKHNGLPCAPLsdvqladpllqeyley----rrvkadsaresegraENYYAVPLSLATRLIKKRh 254 Leishmania braz...
XP_001023701 141 -------sGQHHANFFSDENLKIKTIsdelfkqafpkieatfssikikkndseveqinikkDNFKMFKFIDHPSMLSNNd 213 Tetrahymena the...
XP_001300881 137 --------RYALDQHAKLHEEDGQPFkfpqelleiphp------------klnldqhynrkTKMYTIPFELVFEFINVYt 196 Trichomonas vag...
XP_001827849  98 mntyefnhFYNTKFISKFNNLTESTItnnikkekqvqkis--------kygqydpdfdippINYEFIHFSKCSHTMSKR- 168 Enterocytozoon ...
Q8SRV4        98 -------nPGGARAFFLSEVLPHMDGartqdgvlvlgmr----------srynpdvvasaaSSEVLVHFTKVIDLIGKRa 160 Encephalitozoon...
XP_001306374 124 sy---sspKQIPDFFWPSKELKDQIIns-------------------------------qkDDYYCVPFEQIFDQIDVRt 169 Trichomonas vag...
Q517X5       153 ------lsLEALSQFLVKFGYHPQQItvdeegfl--------------------kqqnyfqGPFIKVPFFEAGKNLKNRr 206 Entamoeba histo...
Feature 1         ######                                                                         
Q8NIZ4       222 vFLRQGYAYVPARE--QQAMVVSEFSSRLERQLELTara---lpRLDEDDRLTPILAHLSKnfitpdasyvgtssaissa 296 Neurospora cras...
Q55BM5       191 aIIIKGEAYIYISN--FKDMVIHTFKNYMNFALDRIkad--ktrIKEEFPELIQFFTTLPKagdgtnkpql------gnt 260 Dictyostelium d...
XP_822563    253 vLCLRGHAILHRDQ--VQEVFVTMFLSKLNKGLHEAylarmklsSHEDHDERETVMKMLDAfleyfiedtmnev-qeasa 329 Trypanosoma bru...
XP_001561600 255 vLCRAGQAILFRDQ--VQEVFLAVFCARLNRGLHSAylarvkqqALEEETAKSTVMAMLDAflqqfvsdpvdsl-qegva 331 Leishmania braz...
XP_001023701 214 vVLHKGYIIIYKES--TSKIVQNIFIERLLDEMRLLqlkf-qnnGSKLDDDRLSFLKDLHKaeifndstqf-------ns 283 Tetrahymena the...
XP_001300881 197 cRLSDGFVEVSPKE--LDSFFTKLYQIYLEKRIARYr------aAKIHSSDLTKIAISLFDeragsfvaks------rqn 262 Trichomonas vag...
XP_001827849 169 -KIKLVQGYLPSTPetQKDFVITEFNNFIEREMHKLs------nNSIRDERLIRLHSDLFIscknssd------------ 229 Enterocytozoon ...
Q8SRV4       161 vVPEGGYLRLNDEG--IGSLLVSEFRRYLSNRMDELve----isSNEPDERMRVLSTSLLAnptgye------------- 221 Encephalitozoon...
XP_001306374 170 iDVENGIAKLTTDH--VYQIVQKNFNDFLVKKLHSAef-----sNLDFISYLRSEFKRVTKseddqi------------- 229 Trichomonas vag...
Q517X5       207 lLLYHGSAFIEKNE--VIDMVIMKNREKLQERVKNNyis---paLGKVPYELKPILEYYQTlvmkitglktat--tgfhv 279 Entamoeba histo...
Feature 1                                                                                        
Q8NIZ4       297 disarniDTLVNNHHFPACMSHLHRTLRRDAHLKHygrlqYTLFLKGIGLNLEECLLFWRQSFNkit--------ddtFN 368 Neurospora cras...
Q55BM5       261 khirkedVSPLSKTSFPMCMRVMYDSLVETSSLKYegrlqFGTFLKGIGLPYDEAINFWRIAFSkrvs-------ntdFD 333 Dictyostelium d...
XP_822563    330 gavgagdVRHLAQTHFPLCMRQVDEHLRREGHLKHqgrftYGLFLKAIGLSMQDSMVLFSSLMTlkagaagkgdpesfAK 409 Trypanosoma bru...
XP_001561600 332 gtvragdVQQLAQTHFPPCMRAVDSHLRREGHLKHhgrftYGLFLKAIGLSLEDSLELFATLMKvkggg----sveafSK 407 Leishmania braz...
XP_001023701 284 tqihhyeLDRLAKRDMPACMTYLMYGLNQKLHLKHfgrlqLGLFLKGAGLSLNEALTFWQKKFSkts--------addFK 355 Tetrahymena the...
XP_001300881 263 fsrvtlqDMDAVIRSFPPCMRSMYDALKKNHKLFHsgrlqFGLYLKGIGLTMDESLKFWRSEFTkais-------lddFE 335 Trichomonas vag...
XP_001827849 230 kgtpnisIKNIYQENFPLCMKLLIGKLEREKHLKHndrnqLSLFLKDLGVPIEDTISYMQSHLYq------------mKN 297 Enterocytozoon ...
Q8SRV4       222 --rgtnfSLESSERYFPPCMQDIMERLRKNKHLKYndrqtLCLFLKDCGMSVEDGIAFFRGSFKapr---------evFD 290 Encephalitozoon...
XP_001306374 230 --ltldnYEELCHRSAPPCIYRILDSLKKSSQLSVkgrfeLCMFLKGLGFDYFSQYIFWKKHFFspkn-------pdlFD 300 Trichomonas vag...
Q517X5       280 etcsksfLKESGIELFPPCMYSIYRKFLHDKHLKHfgllqLIMFLKGVGLSMEDCKKFFEEIEP--------------LS 345 Entamoeba histo...
Feature 1                                                                                        
Q8NIZ4       369 KEYRYNVRHTYGDVGGdsnrrGGGYSPYSCQKILTeh-ppGPGEAHGCPYRHFNMENLqtllqqgmg----------vtd 437 Neurospora cras...
Q55BM5       334 KEYLYNIRHNYGLEGK-----STNYSPYSCPRIISksphnGDKLVHGCPYIQSLERLEqklldlg--------------i 394 Dictyostelium d...
XP_822563    410 TSYGYNIRHNYGMEGK-----KTSYSSLSCTSLLGlppvvDKFDCHGCPFRFKNESAFrgvllkeqlhplgkghssirla 484 Trypanosoma bru...
XP_001561600 408 TAYGYNVRHNYGMEGK-----KTSYSSASCATILGlppvvDQHDCHGCPFRFRDEGALrtmlgketpnpkgrtypsvrpa 482 Leishmania braz...
XP_001023701 356 KKYDYNIRHNYGKLGK-----QLDYTPMSCQKIIGfq--pLKDEFHGCPYKTMESQQLkdflklsyn-----------it 417 Tetrahymena the...
XP_001300881 336 KHYAYNIRHTYGKEGA-----GKNLSAYSCAGILRqp-apASGQTHGCPFKMMQMPQLealikeinp-----------ei 398 Trichomonas vag...
XP_001827849 298 KELVYNIRHNYGLEGK-----KANYSCFSCQKIFSl---sSEPNFTGCPFINNISLAE---------------------- 347 Enterocytozoon ...
Q8SRV4       291 KEYLYSIRHNYGLEGK-----RANYSCFSCSKIANm---tNEERRSSCPFVGDPEHIRerikd----------------- 345 Encephalitozoon...
XP_001306374 301 KQIVPSLKYIYCIDGN-----KKKYQPHSCIALIGqdnpeNPYQVQGCPFRYMMRAELkmyikkmgr-----------ki 364 Trichomonas vag...
Q517X5       346 KERIYNIEHSYGRAGA-----KIDYTGYGCNNLISqi--rNAGDCNGCMMTTKSFKQEdiednllslmelk--gideikr 416 Entamoeba histo...
Feature 1                                                                   
Q8NIZ4       438 rgvlngVKEDKEKQKFHMACNRVFEHLHKeelkkakdegimtaaqletIVHPNEYFKRS 496 Neurospora crassa OR74A
Q55BM5       395 ddfqriQILEATKTSPNVACTKHFNFLHPnntl------------tklINHPNQFYDQS 441 Dictyostelium discoideum AX4
XP_822563    485 asdiedIIQDCKGQHYTRACYKYFMATHPgarr------------dtlFRSPHEYYVTS 531 Trypanosoma brucei TREU927
XP_001561600 483 pgdiedIVSDCKSQHYTRACYKYFMAIHPdarr------------dtlFRSPYEYFSVS 529 Leishmania braziliensis MHOM/BR/75/M...
XP_001023701 418 deqfvqVNIFKNQKQYQLACKEVFKVLNDtrdktkeq---fypyfdkvGNHPNAYFEQS 473 Tetrahymena thermophila SB210
XP_001300881 399 agkymaEINAKARENPGSACAILFNSLHPdhel-----------detgVFHPNVYFNES 446 Trichomonas vaginalis G3
XP_001827849 348 ------QFINIESADPLTNCKKYFVTQLQdknis---------snnllIRSPAEFYKAL 391 Enterocytozoon bieneusi H348
Q8SRV4       346 --vgadIEDIMGEGPFNGKCTRLLEKLTCrkq-------------srlVATPVRYYLEC 389 Encephalitozoon cuniculi
XP_001306374 365 knevisKICEQVPEHPQIACKLFFDGMFEedpl-----------dyagMSHPIQFFKEA 412 Trichomonas vaginalis G3
Q517X5       417 eeilqsVIDLIISYQPTHACTKFLYLSQNkpfdd----------eiqdIIHPNTYFLRA 465 Entamoeba histolytica HM-1:IMSS

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