1B4U


Conserved Protein Domain Family
Extradiol_Dioxygenase_3A_like

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cd07321: Extradiol_Dioxygenase_3A_like 
Click on image for an interactive view with Cn3D
Subunit A of Class III extradiol dioxygenases
Extradiol dioxygenases catalyze the incorporation of both atoms of molecular oxygen into substrates using a variety of reaction mechanisms, resulting in the cleavage of aromatic rings. There are two major groups of dioxygenases according to the cleavage site of the aromatic ring. Intradiol enzymes cleave the aromatic ring between two hydroxyl groups, whereas extradiol enzymes cleave the aromatic ring between a hydroxylated carbon and an adjacent non-hydroxylated carbon. Extradiol dioxygenases can be divided into three classes. Class I and II enzymes are evolutionary related and show sequence similarity, with the two domain class II enzymes evolving from the class I enzyme through gene duplication. Class III enzymes are different in sequence and structure and usually have two subunits, designated A and B, which form a tetramer composed of two copies of each subunit. This model represents subunit A of class III extradiol dioxygenase enzymes. The A subunit is the smaller, non-catalytic subunit. Enzymes that belong to this family include Protocatechuate 4,5-dioxygenase (LigAB) A subunit, 2'-aminobiphenyl-2,3-diol 1,2-dioxygenase (CarB) A subunit, Gallate Dioxygenase and proteins of unknown function.
Statistics
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PSSM-Id: 153390
View PSSM: cd07321
Aligned: 13 rows
Threshold Bit Score: 79.6605
Threshold Setting Gi: 5821792
Created: 6-Jan-2010
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
dimer interfacetetramer
Conserved site includes 18 residues -Click on image for an interactive view with Cn3D
Feature 1:dimer interface [polypeptide binding site]
Evidence:
  • Structure:1B4U; Interface between subunits A (1B4U_A) and B (1B4U_B) of Sphingomonas paucimobilis LigAB heterodimer; contacts at 4A.
    View structure with Cn3D
  • Comment:The active site is located at the intersubunit interface. Most active site residues are from the B subunit.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1        #   #     #  #  #    #                             #  #     ####  #             
1B4U_A        31 ARKGYn--LNQFAMSLMKAENRERFKADESAYLdeWNLTPAAKAAVLARDYNAMIDEGg-NVYFLSKLFstd-------- 99  Sphingomonas pa...
NP_758568      2 ARYEV---DRLIQDMSKKEGLIGRVIDTPSDVFeeYGLTPPERTALLEGTPQALASIGv-HPILQMHYLmyk-------- 69  Pseudomonas res...
YP_001264788   2 SLYQL---SKLLYELNRDDALKARFREAPESVAdgYALTGEERTALLQPDIGLLYVLGv-NGQILMHYAa---------- 67  Sphingomonas wi...
YP_001561135   2 SLYTM---QKFLFQLNREPEVQRRFAEDRAALLaeYALEPEEYEAMFHGDIGKLYVLGc-NGQLLMHFAp---------- 67  Delftia acidovo...
YP_002801324   2 SRNVL---ERVLWQLSVERAAKERFREEPRKFLgrFALSPEEVDMVVDFDVAALQRLGv-SPMLTMGFWqelspsrdmrl 77  Azotobacter vin...
YP_002546766   2 SRYEL---NKALWNYAREPDFKSRFDADAASAVatRSLSDAERSALTDRDIRAIFELGa-HPFLLYSFAia--------- 68  Agrobacterium r...
BAE79500       2 SVPEL---NRLLEDALVREAAWDAVSRLDPQHLsqYDLDFSDIAVLETPDPGKLAAFGv-HPMLAMWGSfm--------- 68  Nocardioides ar...
YP_001152767   3 SRNEKfkiESLLRDFHLKPEVRERFKKEPDKVMqeYGIDPKYWPLLKGGKLRTLYQMGi-HQLLLYHLAh---------- 71  Pyrobaculum ars...
ZP_05743776    2 MSQEF---KKLIEQLQDDPKIISKFLADPVAFLekLHITSEERKALLSRDVEALNVLGl-NDSQAVGAL----------- 66  Lactobacillus i...
YP_618469      1 MSIEF---QKLIQKLQSDPITIGNFLADPEEFLksTKLTNQEKKTLLARDVEALNDLGl-TIDQAVGAL----------- 65  Lactobacillus d...
YP_286449      2 SRNVL---EKLMHQLCVDRSVKQRFKEDLEGLLsrYVLTEEEKAMVRNFDVAGMQKHGv-NAMLTLGFWsen-------- 69  Dechloromonas a...
ZP_01012706   11 TRAYLm--DKVLYELHHKPDHLEAYNADADAYLarFGLPEELATAIKSNDVAGMYLSGv-NPYLLRAHCi---------- 77  Rhodobacterales...
Q5NTE5       315 AVKGFr--INHFLHRLIEPDFRKRFVEDPEGLFaeSDLTEEEKSLIRNRDWIGMIHYGviFFMLEKMAAv---------- 382 Sphingomonas pa...
Feature 1             ##  #  ##
1B4U_A       100 --gkSFQFAAGSMT 111 Sphingomonas paucimobilis
NP_758568     70 --npEMATHVSIKD 81  Pseudomonas resinovorans
YP_001264788  68 ---fCGIEWSDYLQ 78  Sphingomonas wittichii RW1
YP_001561135  68 ---lLGMPWADYLQ 78  Delftia acidovorans SPH-1
YP_002801324  78 ykerLGATDNEYAG 91  Azotobacter vinelandii AvOP
YP_002546766  69 ---sNGGWCMEMMK 79  Agrobacterium radiobacter K84
BAE79500      69 ---rNPDFSAGMSA 79  Nocardioides aromaticivorans
YP_001152767  72 ---vLGIKRDEYVR 82  Pyrobaculum arsenaticum DSM 13514
ZP_05743776   67 ----SGAHSQRCST 76  Lactobacillus iners DSM 13335
YP_618469     66 ----SGAHSQRCST 75  Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842
YP_286449     70 --aaNRHPAAYMKA 81  Dechloromonas aromatica RCB
ZP_01012706   78 ---gVKIPEAESLA 88  Rhodobacterales bacterium HTCC2654
Q5NTE5       383 ----LGIGNIDVYA 392 Sphingomonas paucimobilis

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