1M65,2ANU,2HNH,2HPI,2HQA,2W9M,2YB1,3DCP,3E0D,3E38,3F2B


Conserved Protein Domain Family
PHP
?
cd07309: PHP 
Click on image for an interactive view with Cn3D
Polymerase and Histidinol Phosphatase domain
The PHP (also called histidinol phosphatase-2/HIS2) domain is associated with several types of DNA polymerases, such as PolIIIA and family X DNA polymerases, stand alone histidinol phosphate phosphatases (HisPPases), and a number of uncharacterized protein families. The PHP domain has four conserved sequence motifs and contains an invariant histidine that is involved in metal ion coordination. PHP in polymerases has trinuclear zinc/magnesium dependent proofreading activity. It has also been shown that the PHP domain functions in DNA repair. The PHP structures have a distorted (beta/alpha)7 barrel fold with a trinuclear metal site on the C-terminal side of the barrel.
Links
?
Statistics
?
PSSM-Id: 213985
Aligned: 11 rows
Threshold Bit Score: 43.5696
Created: 12-Dec-2003
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
Download Cn3D
 
active site
Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:The active site of PHP is characterized by a trinuclear metal cluster liganded by histidines, aspartate and glutamate.
  • Structure:2W9M; Deinococcus radiodurans PolX PHP domain binds 3Zn2+ ions
  • Structure:1M65; Escherichia coli YcdX PHP domain binds Zn2+ and 2Na2+ ions
  • Structure:2YB1; Bifidobacterium adolescentis Amidohydrolase PHP domain binds 3Mn2+ ions
  • Structure:3DCP; Listeria monocytogenes HisK PHP domain binds Zn2+ and 2Fe3+ ions
  • Structure:2ANU; Thermotoga maritima HisPPase PHP domain binds 4Zn2+ and 2Cl- ions
  • Structure:3E38; Bacteroides vulgatus HisPPase PHP domain binds 3Zn2+ ions
  • Structure:3F2B; Geobacillus kaustophilus PolC PHP domain binds 2Mg2+ ions
  • Structure:2HPI; Thermus aquaticus PolIIIA PHP domain binds 2Zn2+ ions
Scroll to Sequence Alignment Display
Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection: 
Feature 1         # #                                 #                                        
1M65_A       4 VDLHMHTVAStha-ystLSDYIAQAKQKGi--kLFAITDHgpdmedaphhwhf--------------------inmriwp 60   Escherichia coli
2ANU_A      22 CDFHVHTNXSdgh--lpLGEVVDLFGKHGv--dVVSITDHivdrrtleqrkrngeplgaite-dkfqdylkrlwreqkra 96   Thermotoga marit...
2HNH_A       7 VHLRVHSDYSmidglakTAPLVKKAAALGm--pALAITDFtnlcglvkf----------------------------yga 56   Escherichia coli
2HPI_A       8 AHLHQHTQFSlldgaakLQDLLKWVKETTpedpALAMTDHgnlfgavef----------------------------ykk 59   Thermus aquaticus
2HQA_A       7 VHLRVHSDYSxidglakTAPLVKKAAALGx--pALAITDFtnlcglvkf----------------------------yga 56   Escherichia coli
2W9M_A     329 GMIHTHSTWSdgg--asIREMAEATLTLGh--eFLGTADHsraayyangltierl----------------reqlkeire 388  Deinococcus radi...
2YB1_A       4 IDLHFHSRTSdga--ltPTEVIDRAAARAp--aLLALTDHdctgglaea----------------------------aaa 51   Chromobacterium ...
3DCP_A       3 RDGHTHTEFCphgthddVEEXVLKAIELDf--dEYSIVEHaplssefxkntagdkeavttasxaxsdlpyyfkkxnhikk 80   Listeria monocyt...
3E0D_A       8 AHLHQHTQFSllngaakLQDLLKWVKETTpedpALAMTDHgnlfgavef----------------------------ykk 59   Thermus aquaticus
3E38_A      21 CDFHXHSVFSdgl--vwPTVRVDEAYRDGl--dAISLTEHieyrphkqdvvsdhn----------------rsfdlcreq 80   Bacteroides vulg...
3F2B_A     117 VELHLHTPMSqmdavtsVTKLIEQAKKWGh--pAIAVTDHavvqsfpea----------------------------ysa 166  Geobacillus kaus...

Feature 1                  #                                                                   
1M65_A      61 rvvdgVGILRGIEANiknvdgeidcsgkmfdsldliiagf---------------------------------------- 100  Escherichia coli
2ANU_A      97 weeygXILIPGVEITnntdlyhivavdvkeyvd----------------------------------------------- 129  Thermotoga marit...
2HNH_A      57 ghgagIKPIVGADFNvqcdll----------------------------------------------------------- 77   Escherichia coli
2HPI_A      60 atamgVKPIIGYEAYvaaesrfdrkr------------------------------------------------------ 85   Thermus aquaticus
2HQA_A      57 ghgagIKPIVGADFNvqcdll----------------------------------------------------------- 77   Escherichia coli
2W9M_A     389 lqragLPIVAGSEVDilddgsldfpddvlgeldyvvvsv----------------------------------------- 427  Deinococcus radi...
2YB1_A      52 aarrgIPFLNGVEVSvswgrhtvhivglgidpaepalaaglksiregrlerarqmgasleaagiagcfdgamrwcdnpem 131  Chromobacterium ...
3DCP_A      81 kyasdLLIHIGFEVDyligyedftrdflneygpqtddgvlslhflegqggfrsid------------------------- 135  Listeria monocyt...
3E0D_A      60 atamgVKPIIGYEAYvaaesrfdrkr------------------------------------------------------ 85   Thermus aquaticus
3E38_A      81 aeklgILLIKGSEITraxapghfnaiflsdsnp----------------------------------------------- 113  Bacteroides vulg...
3F2B_A     167 akkhgMKVIYGLEANivddgvpiaynet---------------------------------------------------- 194  Geobacillus kaus...

Feature 1                                                                                      
1M65_A     101 -----------------------------hepvfaphdkatntqamiatiasgnVHIISHPgnpkyeidvkavaeaaakh 151  Escherichia coli
2ANU_A     130 -------------------------------------pslpveeiveklkeqnaLVIAAHPdrkkqdeehlswylwanxe 172  Thermotoga marit...
2HNH_A      78 ------------------------------------------------gdelthLTVLAANntgyqnltlliskayqrgy 109  Escherichia coli
2HPI_A      86 --------------------------------------------gkgldggyfhLTLLAKDftgyqnlvrlasraylegf 121  Thermus aquaticus
2HQA_A      78 ------------------------------------------------gdelthLTVLAANntgyqnltlliskayqrgy 109  Escherichia coli
2W9M_A     428 -------------------------------hsnftldaarqterliravshplVTVLGHAtgrlllrrpgyaldldavl 476  Deinococcus radi...
2YB1_A     132 isrthfarhlvdsgavkdmrtvfrkyltpgkpgyvshqwasledavgwivgaggMAVIAHPgrydmgrtlierlildfqa 211  Chromobacterium ...
3DCP_A     136 --------------fsaedynegivqfyggfeqaqlaylegvkqsieadlglfkPRRXGHIslcqkfqqffgedtsdfse 201  Listeria monocyt...
3E0D_A      86 --------------------------------------------gkgldggyfhLTLLAKDftgyqnlvrlasraylegf 121  Thermus aquaticus
3E38_A     114 ------------------------------------leqkdykdafreakkqgaFXFWNHPgwdsqqpdttkwwpehtal 157  Bacteroides vulg...
3F2B_A     195 ------------------------------------------hrrlgsgsgpfhVTLLAQNetglknlfklvslshiqyf 232  Geobacillus kaus...

Feature 1                                                                                      
1M65_A     152 qvaleinn--------------------------------------------------------ssflhsrkgsedncre 175  Escherichia coli
2ANU_A     173 rfkd----------------------------------------------------------------tfdaweianrdd 188  Thermotoga marit...
2HNH_A     110 gaagpiidrdwlielneglillsggrmgdvgrsl-----lrgnsalvdecvafyeehfpdryflelirtgrpdeesylha 184  Escherichia coli
2HPI_A     122 yekpridreilrehaqglialsgclgaeipqfi------lqdrldlaearlnedlsifgdrffieiqnhglpeqkkvnqv 195  Thermus aquaticus
2HQA_A     110 gaagpiidrdwlielneglillsggrxgdvgrsl-----lrgnsalvdecvafyeehfpdryflelirtgrpdeesylha 184  Escherichia coli
2W9M_A     477 gaceangt---------------------------------------------------------vveinanaarldldw 499  Deinococcus radi...
2YB1_A     212 aggqgi------------------------------------------------------------evasgshslddmhk 231  Chromobacterium ...
3DCP_A     202 evxekfrvilalvkkr----------------------------------------dyeldfntaglfkplcgetyppkk 241  Listeria monocyt...
3E0D_A     122 yekpridreilrehaqglialsgclgaeipqfi------lqdrldlaearlnedlsifgdrffieiqnhglpeqkkvnqv 195  Thermus aquaticus
3E38_A     158 yqegcx------------------------------------------------------------hgievanghlyxpe 177  Bacteroides vulg...
3F2B_A     233 hrvpriprsvlvkhrdgllvgsgcdkgelfdnliqkapeevediarfydflevhppdvykpliemdyvkdeemikniirs 312  Geobacillus kaus...

Feature 1                      # # 
1M65_A     176 vaaavrdaggwVALGSDSHT 195  Escherichia coli
2ANU_A     189 lfnsvgvkkyrYVANSDFHE 208  Thermotoga maritima MSB8
2HNH_A     185 avelaearglpVVATNDVRF 204  Escherichia coli
2HPI_A     196 lkefarkyglgMVATNDGHY 215  Thermus aquaticus
2HQA_A     185 avelaearglpVVATNDVRF 204  Escherichia coli
2W9M_A     500 realrwrerlkFAINTDAHV 519  Deinococcus radiodurans
2YB1_A     232 falhadrhglyASSGSDFHA 251  Chromobacterium violaceum
3DCP_A     242 ivtlaselqipFVYGSDSHG 261  Listeria monocytogenes str. 4b H7858
3E0D_A     196 lkefarkyglgMVATNNGHY 215  Thermus aquaticus
3E38_A     178 aiqwcldknltXIGTSDIHQ 197  Bacteroides vulgatus ATCC 8482
3F2B_A     313 ivalgekldipVVATGNVHY 332  Geobacillus kaustophilus

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap