2O12,1QXO,1Q1L,1UM0,1SQ1,1R53


Conserved Protein Domain Family
Chorismate_synthase

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cd07304: Chorismate_synthase 
Click on image for an interactive view with Cn3D
Chorismase synthase, the enzyme catalyzing the final step of the shikimate pathway.
Chorismate synthase (CS; 5-enolpyruvylshikimate-3-phosphate phospholyase; 1-carboxyvinyl-3-phosphoshikimate phosphate-lyase; E.C. 4.2.3.5) catalyzes the seventh and final step in the shikimate pathway: the conversion of 5- enolpyruvylshikimate-3-phosphate (EPSP) to chorismate, a precursor for the biosynthesis of aromatic compounds. This process has an absolute requirement for reduced FMN as a co-factor which is thought to facilitate cleavage of C-O bonds by transiently donating an electron to the substrate, having no overall change its redox state. Depending on the capacity of these enzymes to regenerate the reduced form of FMN, chorismate synthases are divided into two classes: Enzymes, mostly from plants and eubacteria, that sequester CS from the cellular environment, are monofunctiona,l while those that can generate reduced FMN at the expense of NADPH, such as found in fungi and the ciliated protozoan Euglena gracilis, are bifunctional, having an additional NADPH:FMN oxidoreductase activity. Recently, bifunctionality of the Mycobacterium tuberculosis enzyme (MtCS) was determined by measurements of both chorismate synthase and NADH:FMN oxidoreductase activities. Since shikimate pathway enzymes are present in bacteria, fungi and apicomplexan parasites (such as Toxoplasma gondii, Plasmodium falciparum, and Cryptosporidium parvum) but absent in mammals, they are potentially attractive targets for the development of new therapy against infectious diseases such as tuberculosis (TB).
Statistics
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PSSM-Id: 143612
View PSSM: cd07304
Aligned: 143 rows
Threshold Bit Score: 345.172
Threshold Setting Gi: 229849049
Created: 30-Sep-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 15 residues -Click on image for an interactive view with Cn3D
Feature 1:FMN-binding site [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                        
2O12_A         2 LRWITAGESHGRALVAVVEGMVAGVHVTSADIADQLARRRLGyGRGARMTFERDAVTVlSGIRHGSTLGGPIAIEIGNTE 81  Mycobacterium t...
A4XKJ4         1 MRFLDAGETHGRCLVGIIEGFPANVKINIDNINRLLELRQRGyGRGKRMEIEKDKATIlSGVRNSYTTGAPITIMIENRD 80  Caldicellulosir...
ZP_02210436    4 LRYLTSGESHGQSLISIIDGLPSNVELNFDEINAELKKRQCGyGRGGRMKIESDKVNIlGGVRGKKTLGGPVSIEVKNRD 83  Clostridium bar...
YP_001956226   2 IRFTTAGESHGEGLFVIIEGIPAGLTINSEDIDLELARRQKGyGRGQRMCIETDTVKVfSGMRHTVSLGSPIAMYIANKD 81  uncultured Term...
Q8R9A6         1 MRYLTAGESHGEALIAIIEGLPSNLFIDAEFINKELERRQKGyGRGGRMAIEKDEIHIiSGVRDGKTTGAPLAMEIKNRD 80  Thermoanaerobac...
Q24UW1         1 MRYLTAGESHGPKLVGILEGVPSGAKIDKETIDQALQERQKGpGRGGRMKIEKDQVTIlSGVRGGLTTGAPIALEIINRD 80  Desulfitobacter...
CAI50957       5 LRYYSAGESHGRGIFAFLDGFPAGVRVQPDDINFWLAERQKGyGRGGRQRIEKDEVDVlSGIRGGVTLGSPILLAVWNRD 84  uncultured bact...
ZP_01091428    2 LRYSTAGESHGKTLLALVDGFPAGVEIETDAIDADLKLRQGGyGRGGRQRIETDKVEVlTGIWKGLSLGSPIALQVVNKD 81  Blastopirellula...
NP_782665      7 LRFLDAGESHGKAMMSIIDGIPSNFKVDIDFINNELKRRQKCyGRGGRMKIEKDKIQFlSGLRGTMTTGNPITMAIYNND 86  Clostridium tet...
Q9WYI2         1 MKLTIAGDSHGKYMVAILEGLPSGIRVDEELIRRDLFRRRNCyGRGKRMKMEEDAFEIvSGLWKGITTGAPVTILIPNRA 80  Thermotoga mari...
Feature 1                                        ###                  # #                        
2O12_A        82 WPKWETvmaadpvdpaeladvarnaplTRPRPGHADYAGMLKYGfdDARPVLERASARETAARVAAGTVARAFLRqalgV 161 Mycobacterium t...
A4XKJ4        81 YVNWQKymdpilc-------dtmtkkvTVPRPGHADLPGCLKYGfdDARPVLERASARETAMRVAIGALCEELLNvf-gI 152 Caldicellulosir...
ZP_02210436   84 YQNWTEymapmddv------dfetrkvDNVRPGHADLVGCLKYDftDARNVLERSSARETASRVAVGAICKQVLKnf-dI 156 Clostridium bar...
YP_001956226  82 FKNWTKimsptsv-------dsevtplLRPRPGHADLPGFMKYGvnDFRDILERASARETAARVAAGAVCKALLKef-eI 153 uncultured Term...
Q8R9A6        81 YKNWKDkkv---------------ppvTRPRPGHADLPGSIKYNqrDIRNILERASARETAARVAVGSVAKLLLKel-nI 144 Thermoanaerobac...
Q24UW1        81 WANWEKimawgdea------dlesrkvITPRPGHADLTGHLKYRt-EVRNVLERASARETAMRVAIGNIAVQILEal-gV 152 Desulfitobacter...
CAI50957      85 FKNWESamdpwkps-----tgerarkvVRPRPSHADLVGALKYEhdDCRNILERASARETAARVAAGALCRKLLAef-gV 158 uncultured bact...
ZP_01091428   82 YKLERLed------------------lPRPRPGHGDLTGAIKYLg-PVRGILERASARETAVRVAAGGLAKLLLKqf-gI 141 Blastopirellula...
NP_782665     87 SPNWEKilsgev---------kkdekiTIPRPGHGDLVGYFKYGtgDIRDSIERTSARETSIRTAVGALCKQILKgi-gI 156 Clostridium tet...
Q9WYI2        81 GNPVKDv-------------------rSVPRPGHIDYAAWVKYKlpDLNIYVERSSARWTVALTAAGSLLKSLLKef-gI 140 Thermotoga mari...
Feature 1                                                                                        
2O12_A       162 EVLSHVISIGasapyegppprae-dlpaidaspvrayDKAAEADMIAQIEAAKKDGDTLGGVVEAVAlGLPVGLGSFTSg 240 Mycobacterium t...
A4XKJ4       153 KLYNHVVEIGgvrikkeystddvnlfeeaensdlfciDKEAENDMKQVIDSAKEAGDSVGGVAEVICkNVPFGLGSHVHw 232 Caldicellulosir...
ZP_02210436  157 DFVSHVIQIGnvkdenvydfdyi--knnvdssevrcaNKDMEAKMISQIDEIKNERDTIGGVVEVRVkNLIPGLGSYTQf 234 Clostridium bar...
YP_001956226 154 SIVSYTSQIGdvtadissveeck-iwhdaeiscvrcpDAKASEKMIQLIKKAGEKGDTLGGKVVVVTkNVPAGLGSHTQw 232 uncultured Term...
Q8R9A6       145 SLKSRVLEIG---------------------------GAKREEKWKRLIEKAKKEGDTLGGIIEIVIeGVPVGLGSHAQw 197 Thermoanaerobac...
Q24UW1       153 EIRGQVLSVGkvhmnsedtpey---wqrvqasewkvgDPQGEEALYTQLQEARSKGESLGGVLQIQVqNLLPGLGSYVQw 229 Desulfitobacter...
CAI50957     159 DMVGHVVQIGeaaadtdriptae-lrersmasdvrcaDAEAGTRMREAIRQAKRDKDSLGGIIEVRAwGLPAGLGSHTQa 237 uncultured bact...
ZP_01091428  142 TAFGYVAELGgvqitpqagdlek-lrklrsdsivyslNPDQDAEIKQLIDATGKSGDTLGGIVEVRVdGVPFGLGTHSQw 220 Blastopirellula...
NP_782665    157 EVRSKVYSIGnlfdekvdlfdqc-kykkidnsllrcyNEEVEKSFVKKIDICREQGETIGGTVFLSVrGVPIGLGSYSQw 235 Clostridium tet...
Q9WYI2       141 EVLGFVTRLGnveardipgdfee-lkrrrdesvvfcpDPEATKEMVAEIDRAKEEGNTLGGKVKVIArGVPAGIGSYSDl 219 Thermotoga mari...
Feature 1                      ###                                                               
2O12_A       241 dHRLDSQLAAAVMGIQAIKGVEIGd-----GFQTArrrGSRAHDEmypgpd-------gvvrsTNRAGGLEGGMTNGQPL 308 Mycobacterium t...
A4XKJ4       233 dRKLDGLLAQAVMSIQSVKGVEIGm-----GFEVSrrfGSEVHDEifyddq------kgfyrkTNNAGGIEGGISNGMDI 301 Caldicellulosir...
ZP_02210436  235 dRKIDGVMAMHLMGIQAIKGVEFGi-----GFDVAklpGSKVMDEivynqe------agikrvTNRLGGIEGGMTTGEEL 303 Clostridium bar...
YP_001956226 233 dLKLDGRLAQSLISIQAVKAVEFGa-----GTKLAsllGALSHDEifydvt------rgfyrdTNRAGGIEGGMSNGEPI 301 uncultured Term...
Q8R9A6       198 dRKLDALLAYHVMSVQGIKGVEFGl-----GFEAArlpGSLVHDDiyyken------egfyrkTNNAGGIEGGMSNGNPI 266 Thermoanaerobac...
Q24UW1       230 dRKLDGRLAQAVLSVQAIKGVAFGm-----GFAAGqhfGSEVHDPigydsg------rgyyrySNNAGGIEGGMTNGEPV 298 Desulfitobacter...
CAI50957     238 dRKLDAAIAQAMMSIQAVKGVEVGi-----GFRGAhlrGSAYHDEivwseapk--ggshyvrtSNHLGGTEGGMSSGEEL 310 uncultured bact...
ZP_01091428  221 dRKLDGRLAQAVMAVQAIKGVEIGl-----GFEAArrpGSQVHDPihfdpehrdqphlgfvrpTNNAGGLEAGMTNGQPV 295 Blastopirellula...
NP_782665    236 dRKLDALLSYAIMSLQGVKAIEFGn-----GMNLNl-rGSTFNDEilyekg-------kfkrpTNNCGGIESGVSNGENI 302 Clostridium tet...
Q9WYI2       220 fKKLDSKIGSLFFAIPAVKGVVIGseemwyGFDYLd--EFELEDGkik-------------rkTNNLGGIEGGITNGEDV 284 Thermotoga mari...
Feature 1              # ###                       #  #  #                
2O12_A       309 RVRAAMKPISTVPRalatvdlatgdeaVAIHQRSDVCAVPAAGVVVETMVALVLARA 365 Mycobacterium tuberculosis H37Rv
A4XKJ4       302 VIRAAFKPIPTLYKplrsvdlqglkekEAAVERSDTCAVPAGSVVMRAAVAYVLANS 358 Caldicellulosiruptor saccharolyticus D...
ZP_02210436  304 VVRCAMKPIPTLYKplnsinvntlekyEATVERSDTCAVPACSVVCENVVAFVICQF 360 Clostridium bartlettii DSM 16795
YP_001956226 302 VVTCTMKAIPSLANplhsvnlatketaEAEAIRSDVCAVPAVGIVAEAAIAVELAKA 358 uncultured Termite group 1 bacterium p...
Q8R9A6       267 VIRAAMKPIPTLLRpldsvdiatkeetKAIYERSDVTAVEAAACVLEAVCAWVIADE 323 Thermoanaerobacter tengcongensis
Q24UW1       299 IIEAVMKPIPTLYSplstvnletkevmEASVERSDVCAVPAALVVLKHVAAWEILQA 355 Desulfitobacterium hafniense Y51
CAI50957     311 LVRIVKKPISTLMRplrtvnietrqpdAALVERSDTCAVPALALIAEHAVAIVLAQF 367 uncultured bacterium
ZP_01091428  296 IIRAAKKPISTLAKpldsinldtkekqGASYERSDVCAVSAAAIIVENVVAFEIATA 352 Blastopirellula marina DSM 3645
NP_782665    303 EMKVYIKPIPSIKKnirtvnlrnrketTTRYERSDVSAVVPASIVLENIVAFEILKE 359 Clostridium tetani E88
Q9WYI2       285 WVNVSVKPIPTTGKplksvdlrtmepaKTPYVRSDVTAVPPASVVCEAALAVVISDA 341 Thermotoga maritima

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