Conserved Protein Domain Family
PX_SNX30

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cd07283: PX_SNX30 
The phosphoinositide binding Phox Homology domain of Sorting Nexin 30
The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. Some SNXs are localized in early endosome structures such as clathrin-coated pits, while others are located in late structures of the endocytic pathway. SNX30 harbors a Bin/Amphiphysin/Rvs (BAR) domain, which detects membrane curvature, C-terminal to the PX domain, similar to the sorting nexins SNX1-2, SNX4-8, and SNX32. Both domains have been shown to determine the specific membrane-targeting of SNX1. The specific function of SNX30 has yet to be elucidated.
Statistics
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PSSM-Id: 132816
View PSSM: cd07283
Aligned: 4 rows
Threshold Bit Score: 225.35
Threshold Setting Gi: 148227022
Created: 26-Jan-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
phosphoinosit..
Feature 1:phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:A majority of PX domain containing proteins binds phosphatidylinositol-3-phosphate (PI3P) at this site. In some cases, other phosphoinositides, such as PI4P or PI(3,4)P2, are the preferred substrates.
  • Comment:based on the structures of phosphatidylinositol-3-phosphate bound to other members of this superfamily
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                 ###                       ##           
XP_424910     92 LFVTVDDPKKHVCTMETYITYRVTTKTTRAEFDLPEYSVRRRYQDFDWLRNKLEESQPTHLIPPLPEKFVVKGVVDRFSE 171 chicken
NP_001089520 106 LFVTVDDPKKHVCTMETYITYSVSTKTTRTEFDLPEYSVRRRYQDFDWLRNKLEETQPTHFIPPLPEKFVVKGVVDRFSE 185 African clawed ...
NP_001017798  82 LFVTVDDPKKHVSTMETYITYRVCTKTTRTEFDLPEYSVRRRYQDFDWLRIKLEDSQPTHLIPPLPEKFVMKGVVDRFSE 161 zebrafish
NP_001013012  91 LFVIVDDPKKHVCTMETYITYRITTKSTRVEFDLPEYSVRRRYQDFDWLRSKLEESQPTHLIPPLPEKFVVKGVVDRFSE 170 human
Feature 1             #                              
XP_424910    172 EFVETRRKALDKFLKRITDHPVLSFNEHFNVFLTAK 207 chicken
NP_001089520 186 EFVETRRKALDKFLKRIADHPVLSFNEHFNVFLTAK 221 African clawed frog
NP_001017798 162 EFVETRRKALDKFLKRVADHPVLSFNEHFNAFLSAK 197 zebrafish
NP_001013012 171 EFVETRRKALDKFLKRITDHPVLSFNEHFNIFLTAK 206 human

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