Conserved Protein Domain Family
PX_RUN

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cd07277: PX_RUN 
The phosphoinositide binding Phox Homology domain of uncharacterized proteins containing PX and RUN domains
The PX domain is a phosphoinositide (PI) binding module involved in targeting proteins to PI-enriched membranes. Members in this subfamily are uncharacterized proteins containing an N-terminal RUN domain and a C-terminal PX domain. PX domain harboring proteins have been implicated in highly diverse functions such as cell signaling, vesicular trafficking, protein sorting, lipid modification, cell polarity and division, activation of T and B cells, and cell survival. In addition to protein-lipid interaction, the PX domain may also be involved in protein-protein interaction. The RUN domain is found in GTPases in the Rap and Rab families and may play a role in Ras-like signaling pathways.
Statistics
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PSSM-Id: 132810
View PSSM: cd07277
Aligned: 12 rows
Threshold Bit Score: 182.162
Threshold Setting Gi: 157119104
Created: 13-Jan-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
phosphoinosit..
Feature 1:phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:A majority of PX domain containing proteins binds phosphatidylinositol-3-phosphate (PI3P) at this site. In some cases, other phosphoinositides, such as PI4P or PI(3,4)P2, are the preferred substrates.
  • Comment:based on the structures of phosphatidylinositol-3-phosphate bound to other members of this superfamily
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                         ###                       ##    
EAW85124      708 INVWIPSVFLRGKa---aNAFHVYQVYIRIk---------DDEWNIYRRYTEFRSLHHKLQNKYPqvRAYNFPPKKaiGN 775  human
XP_786538     819 INLWIPSAFLRGKg---kDAFHVYQIYIRIr---------DDEWNVYRRYSQFRELHIKMKKSNAviNTFEFPPKKavGN 886  purple urchin
XP_973447     470 VNVWIPSAFLTGTg---sSAHHVYQIFLRAg---------NDEWNIYRRYAQFYALHSDLKKLDPavTSFDFPPKKsiGK 537  red flour beetle
XP_414731     864 INVWIPSVFLRGKa---aNAFHVYQVYIRIk---------DDEWNVYRRYAEFRSLHHKLQNKYQqvRTFNFPPKKaiGN 931  chicken
NP_609607     404 FLITIPHVKLAKTqr--sGSHYTYEVHITMrq-------rLEHWTFFRRYSEFYKLHKSLLKTHPvvSAVEFPPKKhfGN 474  fruit fly
CAG04491      209 INVWIPSVFLQGRa---aNAYHVYQVYIRIq---------DNEWNVYRRYTEFRELHNHLRTQFPqiDAFAFPPKKalGN 276  spotted green...
XP_001606759  546 IHIWIPSAFLTGQp---sDIHHVYQIYVRIr---------DTEWNIYRRYAQFYSLYRELKKHDAivTSFEFPPKKtiGN 613  jewel wasp
XP_001659338  286 FQISIPKAILRTPvsgrrREHYCYEIRISPngtadgghhqPESWSVFRRYSEFYRLHKRLQKEYPsvRTLDFPPKKkiGN 365  yellow fever ...
XP_002202470  753 VNIWIPSVFLRGKa---sDTHHVYQVYIRIr---------DEEWNIYRRYAQFYELHSQMKKKFPsvASLGFPPKKaiGN 820  Florida lancelet
EEC02552      626 INIWIPSAFLAGHr---sDAFHVYQVYVRIr---------DDEWNVYRRYSQFYALHKALRKSNPvvGSFDFPPKKsiGN 693  black-legged ...
XP_002130434  561 ISVWMPSVLLKGRg---pDASHFYQVFIRAg---------DDEWNVYRRYSEFHEFHLQIAKKFPvvETFNFPPKKlvGN 628  Ciona intesti...
XP_001624837  194 ISVWIPSVFMRGKg---sSGVHLYQVYIRIk---------DDEWNVYRRYSQFHDLHKKLCKKRSvvGSFKFPPKKtlGN 261  starlet sea a...
Feature 1                                                               #                         
EAW85124      776 KDAK----------------------------------------------FVEERRKQLQNYLRSVMNKVIQmvpefaas 809  human
XP_786538     887 KDTK----------------------------------------------FVEERRKRLQHYLRLVINFCVQnsleltet 920  purple urchin
XP_973447     538 KDSA----------------------------------------------LVEERRKRLQVYLRRVLAHWPElsh----c 567  red flour beetle
XP_414731     932 KDAK----------------------------------------------FVEERRKQLQNYLRNVMNKVIQtvpeftan 965  chicken
NP_609607     475 MNLV----------------------------------------------FVEERRQQLQIYLLNLVETLPQvea----c 504  fruit fly
CAG04491      277 KILEqlcgfpersslvvlrlfrssssvfaearrfsvgpnligmsqkqdakFVEERRKQLQGYLRMVMNKLIQtlpeftar 356  spotted green...
XP_001606759  614 KDAK----------------------------------------------FVEERRQKLQQWLRRIVNRLSHcspvfssr 647  jewel wasp
XP_001659338  366 MNAQ----------------------------------------------FVEQRRQRLQVYLNSLFITTLPevs---ac 396  yellow fever ...
XP_002202470  821 KDSK----------------------------------------------FVEDRRKRLQNYLRHMMNIVVQntqglvag 854  Florida lancelet
EEC02552      694 KDAK----------------------------------------------VVEERRKRLQRYLRCVLNWMAQtsselles 727  black-legged ...
XP_002130434  629 KSRQ----------------------------------------------FVEERRRSLQSYIRLMINNIIRnhekiisn 662  Ciona intesti...
XP_001624837  262 KGTK----------------------------------------------FVEERRKHLQDYIRRIVNLLTTtdeelsan 295  starlet sea a...
Feature 1                         
EAW85124      810 pKKETLIQLMPFFVDI 825  human
XP_786538     921 pSKETLISLIPFFGDT 936  purple urchin
XP_973447     568 nSRFLLEQHLAFFKDQ 583  red flour beetle
XP_414731     966 pKKETLIQLMPFFVDI 981  chicken
NP_609607     505 kSKAELQKVFPFFRDR 520  fruit fly
CAG04491      357 pTKETLLSLLPFCLCS 372  spotted green pufferfish
XP_001606759  648 pSKQTLIVLMPFFGDF 663  jewel wasp
XP_001659338  397 nTRSQLERAFPFLKDA 412  yellow fever mosquito
XP_002202470  855 aGRERLVAVLPFFGDP 870  Florida lancelet
EEC02552      728 pDKSTLVALLPFFREQ 743  black-legged tick
XP_002130434  663 pTKTTLVTLLPFFSET 678  Ciona intestinalis
XP_001624837  296 tNKAILLKIVPFFGSC 311  starlet sea anemone

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