Conserved Protein Domain Family
PX_SNX16

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cd07276: PX_SNX16 
The phosphoinositide binding Phox Homology domain of Sorting Nexin 16
The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions. Sorting nexins (SNXs) make up the largest group among PX domain containing proteins. They are involved in regulating membrane traffic and protein sorting in the endosomal system. The PX domain of SNXs binds PIs and targets the protein to PI-enriched membranes. SNXs differ from each other in PI-binding specificity and affinity, and the presence of other protein-protein interaction domains, which help determine subcellular localization and specific function in the endocytic pathway. SNX16 contains a central PX domain followed by a coiled-coil region. SNX16 is localized in early and recycling endosomes through the binding of its PX domain to phosphatidylinositol-3-phosphate (PI3P). It plays a role in epidermal growth factor (EGF) signaling by regulating EGF receptor membrane trafficking.
Statistics
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PSSM-Id: 132809
View PSSM: cd07276
Aligned: 9 rows
Threshold Bit Score: 206.491
Threshold Setting Gi: 189235024
Created: 3-Feb-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
phosphoinosit..
Feature 1:phosphoinositide binding site [chemical binding site]
Evidence:
  • Comment:A majority of PX domain containing proteins binds phosphatidylinositol-3-phosphate (PI3P) at this site. In some cases, other phosphoinositides, such as PI4P or PI(3,4)P2, are the preferred substrates.
  • Comment:based on the structures of phosphatidylinositol-3-phosphate bound to other members of this superfamily
  • Comment:Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                ###                       ##            
P57769       105 DRPSTPTILGYEVMEERAKFTVYKILVKKSp-eESWVVFRRYTDFSRLNDKLKEMFPg-fRLALPPKRWFKdNYNADFLE 182 Norway rat
NP_611252    219 NAVLRVPIIGYEVMEERARFTAYKLRVENPetnDYWLVMRRYTDFVRLNSKLKQAFPn-lTLMLPRKKLFGdNFNAVFLD 297 fruit fly
XP_393778     70 SDDLRIPIVGYEVMEERARFTVYKLRVELKn-gDCWFVFRRYTDFVRLLSQLRRQKIpisQLSLPRKKWLGdNFAPSFLE 148 honey bee
NP_001017302  96 ERPSTPTVLGYEVMEERAKFTVYKILVRKSp-eENWVVFRRYTDFSRLNDKLKEMFPg-fRLALPPKRWFKdNYDYDFLE 173 western clawed ...
NP_991228     63 PRPATPTVLGYEVMEERAKFTVYKVLVRKNv-dESWVVFRRYTDFSRLNDKLKDMFPg-fRLSLPPKRWFKdNYETEFLE 140 zebrafish
XP_418313    104 DRPSTPTILGYEVMEERAKFTVYKILVKRSp-eESWVVFRRYTDFSRLNDKLKDMFPg-fRLALPPKRWFKdNYNPDFLE 181 chicken
XP_001639412  60 DNGIRAPITGYEVVERREKFTVYKIQVLCQg--RSWFVFRRYTDFVRLNNKLRQAFSg-fIIKMPGKRFLRdNYDPEFLE 136 starlet sea ane...
XP_971845     91 LNNIQIPIVGYEIMEERARFTVYKLRIENKvsgDCWYVFRRYTDFVRLCNRIRNSHTh-iVQLLPRKRWLKnNFDPLFLE 169 red flour beetle
EEC05154      79 VPEYNTPIIGYEVMEERARFTVFKIRVEHVesgRYWFVFRRYTDFGRLSKKLKPLFPg-lQLSLPPKRWFGnNFDPMFLE 157 black-legged tick
Feature 1         #                              
P57769       183 DRQLGLQAFLQNLVAHkDIANCLAVREFLCLD 214 Norway rat
NP_611252    298 NRVQGLQIFVNSVMAKeELRKCKLVREFFCLD 329 fruit fly
XP_393778    149 GRIRGLQAFVNGILSSpLLIGTACVREFFCLD 180 honey bee
NP_001017302 174 ERQLGLQAFLQNLVAHkDIANCAPVRLFLCLD 205 western clawed frog
NP_991228    141 ERQLGLQTFLQNLVAHkDIASCVAVREFLCLD 172 zebrafish
XP_418313    182 DRQLGLQAFLQNLVAHkDIANCIAVREFLCLD 213 chicken
XP_001639412 137 ERAHGLQMFMNSIMRHsKISRSKLLREFLCID 168 starlet sea anemone
XP_971845    170 ERVSGLQTLVNAILAEpSLITSQEIQDFFCLN 201 red flour beetle
EEC05154     158 DRLLGLQAFVKNIMGHrDISKSSPVREFFCLD 189 black-legged tick

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