Conserved Protein Domain Family
Pat_TGL4-5_like

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cd07230: Pat_TGL4-5_like 
Triacylglycerol lipase 4 and 5
TGL4 and TGL5 are triacylglycerol lipases that are involved in triacylglycerol mobilization and degradation; they are found in lipid particles. Tgl4 is a functional ortholog of mammalian adipose TG lipase (ATGL) and is phosphorylated and activated by cyclin-dependent kinase 1 (Cdk1/Cdc28). TGL4 is 30% homologus to TGL3, whereas TGL5 is 26% homologus to TGL3. This family includes TGL4 (STC1) and TGL5 (STC2) from Saccharomyces cerevisiae.
Statistics
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PSSM-Id: 132868
View PSSM: cd07230
Aligned: 28 rows
Threshold Bit Score: 631.564
Threshold Setting Gi: 74626368
Created: 19-Feb-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
active sitenucleophile
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the crystal structure of semet patatin (1OXW)
  • Comment:catalytic dyad is formed by Ser and Asp
  • Comment:oxyanion hole is formed by two Gly and one Arg

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                         
Q12043        110 LLYLIRTKWVRNLGNMNNvnLYRHSHTGTKQIIHDYLEEsqavltali--hqsnmndhYLLGILQQTRRn-IGRTALVLS 186  baker's yeast
NP_984327     148 LLYIIRTTWVRDLGNMCNvnLYRHSHVGTKYIIDEYVEEskrslhelv--yqsnldmnYMLGMLVQTRKn-IGRTALVLS 224  Ashbya gossyp...
XP_451336     141 LLYLIRTTWVRNRGGMGNvnLYRHSYVGTKHIIEDYVAEssrslqelv--ynsdlddtYLLGMLTQTRKn-IGRTALVLS 217  Kluyveromyces...
XP_505230     152 LLYLVRTTWSRNLGNLGDvkLYHNSFTGTKRLIEDYILEcelalnallaagndkipdqELLTELLNTRKa-FGRTALLLS 230  Yarrowia lipo...
Q9Y827         68 LLGLLRNSVARDFANMDNsrLYNYAHSGTKKLIDEFIQEvlmcltyl---eetpdlslDEKITEFSRLKltTGNTALILS 144  fission yeast
XP_001527475  151 LLYYVRTSWIRNLGNIGDadLYRHSYVGTKKLIEEYLQEcqlalnylvedggsqlddrYLLGMLIQTRKn-IGRTALVLS 229  Lodderomyces ...
XP_001382886   86 LLYYIRTKWIRNLGNMGNinLYRHSLVGTKKLIEEYIDEcqlsldylihdpdvnlddrYLLGMLIQTRKn-IGRTALVLS 164  Pichia stipit...
XP_001645729  172 LVYLIRTNWVRNLGNMGNvnLYRYSHVGTKYLIDDYMNEsrlcieall--hqselddtYLLGMLQQTRRn-IGRTALVLS 248  Vanderwaltozy...
EDK38516      134 LLYLIRTKWTRNIGNMGDigLYRHAYVGTKKLIEEYTQEceealeylingsqvdlddrYLLGMLIQTRKn-IGRTALVLS 212  Pichia guilli...
CAG87780      136 LLYLIRTKWTRNLGNMGDinLYRHSHVGTKTLIEDYIKEcemsldylinnkevslddrYLLGMLIQTRKn-IGRTALVLS 214  Debaryomyces ...
Feature 1          ## #                        #                                                  
Q12043        187 GGSTFGLFHIGVLAALFEsdLMPKVISGSSAGAIVASIFCVHTTQEIPSLLtnvlnmeFNIFNDDNSKSp---------- 256  baker's yeast
NP_984327     225 GGSTFGLFHIGVLSTLFEqeLLPRVISGSSAGAIVASILCVHHKHELVQLIed---vlQKEFNIFEDESqkv-------- 293  Ashbya gossyp...
XP_451336     218 GGGTFGLFHIGILVTLFEqeLLPRVISGSSAGAIVASILASRQEHELMGLMde---ilVKDFNIFKDDLems-------- 286  Kluyveromyces...
XP_505230     231 GGGCLGLLHTGVLQALSDtsLLPHVISGSSAGSIMAAGLCIHKDEEHEAFIte---lmERDFDIFEESGn---------- 297  Yarrowia lipo...
Q9Y827        145 GGGTFGMTHIGVLQSLHEqgLVPKIICGSSAGAIVACAAAVRNKEEQEILLrq---fhTGDLSVFTDPNaa--------- 212  fission yeast
XP_001527475  230 GGSTFGVFHIGVLATLFEanLLPRIISGSSAGSIMASILCSHTNDETVELLqt---iaRRRFNIFDITDydptk---vqg 303  Lodderomyces ...
XP_001382886  165 GGSTFGIFHIGVLATLFEanLLPRIISGSSAGSIVASILCCHSNEDTFELLrt---itERDFNIFSDDEspnyk---srs 238  Pichia stipit...
XP_001645729  249 GGGTFGLFHIGVLSTLSEldILPRVISGSSAGAIVASILSVYHKDELPELFer---vlGTDFNIFKDDSlks-------- 317  Vanderwaltozy...
EDK38516      213 GGSTFGIFHIGVLITLIEenLLPRIISGSSSGSIMASIMCSHTNEETVELLkn---isEREFRIFEINStldreeptkkg 289  Pichia guilli...
CAG87780      215 GGSTFGIFHIGVVITLLEssLLPRIISGSSAGSIIASIICSHSNEETVSILnd---imVRRFDIFESGDiekek---dge 288  Debaryomyces ...
Feature 1                                                                                         
Q12043        257 -nENLLIKISRFCQnGTWFNNQPLINTMLsFLGNLTFREAYNKTGKILNITVSpASIYEQPKLLNNLTAPNVLIWSAVCA 335  baker's yeast
NP_984327     294 khESLLMKISRFLKnGTWFDNKHLINTMIgFLGDLTFREAYNRTGKILSITVSpASVYEQPRLLNQLTAPNVLIWSAVCA 373  Ashbya gossyp...
XP_451336     287 esENLLIKISRFFKnGTWFDNKNLVSTMInFLGNLTFREAYNRSGKILNITVSpASVHEQPRLLNNLTSPNVLIWSAVCA 366  Kluyveromyces...
XP_505230     298 -eDTVLERVSRMLKhGSLLDNRYMQDTMReLFGDMTFLEAYNRTRRILNVTVSsAGIYEMPRLLNYLTAPNVLIWSAVCA 376  Yarrowia lipo...
Q9Y827        213 -pPSVIQSVKQYFTrGCVLDISHLERVMKlLIGDFTFQEAYDRSGYILNVTVScGSLFEMPSLLNYITAPNVLVWSAVVA 291  fission yeast
XP_001527475  304 kfRKVLVFLGHLIKyGTIFDIDGLQETMMgFLGDLTFREAYNRTGKILNITVSpASIHEQTRLLNHLTAPNCLIWSAVCA 383  Lodderomyces ...
XP_001382886  239 nfGKLLDSFAHFIKyGTLYDMTGLKQTMIgFLGDLTFREAYNRTGKILNITVSpASIHESTRLLNYLTAPNCLVWSAVCA 318  Pichia stipit...
XP_001645729  318 esENFLIKFSRFFKnGTWFDNKHLGRTMIeFLSDLTFREAYNRTGKILNITVSpASLFEQPRLLNNLTAPNVLIWSAVCA 397  Vanderwaltozy...
EDK38516      290 nlKNTLECLSHFIKyGTFFDICGLKKTMIdFVGDLTFREAYNRTGKILNITVSpTSIHEQTRLLNYTTAPNCLIWSAVCA 369  Pichia guilli...
CAG87780      289 syKKVLKNLSHLLKyGTLFDIDSLQNTMIgLVGDLTFREAYNRTGRILNITVSpTSIHEQTRLLNYLTAPNCLIWSAVCA 368  Debaryomyces ...
Feature 1                                                #                                        
Q12043        336 SCSLPGVFpSTPLFEKDPHTGKIKEWGatnlhlsnmKFMDGSVDNDMPISRLSEMFNVDHIIACQVNIHVFPLLkfsntc 415  baker's yeast
NP_984327     374 SCSLPGVFpSTPIYEKDPKTGETTEWNss-----svKFVDGSVDNDLPIAKLSEMFNVDHIIACQVNIHAFPFLklslsc 448  Ashbya gossyp...
XP_451336     367 SCSLPGIFpSSPLYEKDPKTGETKQWNss-----svKFVDGSVDNDLPVSKLSEMFNVDHIIACQVNIHVFPFLklsvsc 441  Kluyveromyces...
XP_505230     377 SCSVPLIFnAYTLLEKEPKTGAIQTWNas-----slRFIDGSVYADVPIARLSEMFNVNHFIVSQVNPHVAPFLkltedk 451  Yarrowia lipo...
Q9Y827        292 TCSVPFLFkRATLWERDPLTREVSAFCvtd----apLWMDGSVDNDIPHAKLTELFHVNHFIVSQVNFHIVPFImdptsh 367  fission yeast
XP_001527475  384 SCSVPGVFpSSSVYEKNVKTNEIHEWNnde----lmKYMDGSVENDLPITRLLEMFNVDHIIAVQVNPHVVPIMkvsvsn 459  Lodderomyces ...
XP_001382886  319 SCSLPGVFpSTTIYEKNPKNNEIHEWNnda----amKYVDGSVDNDLPITRLSEMFNVDHIIAVQVNPHVVPVLkvsvsn 394  Pichia stipit...
XP_001645729  398 SCSLPGIFpSSPLYEKDPKTGETREWIgss----svKFVDGSVDNDLPISRLSEMFNVDHIIACQVNIHVFPILklslsc 473  Vanderwaltozy...
EDK38516      370 SCSLPGVFpSTTIYEKIPKTNQIQEWNndt----smKFVDGSMENDLPIARLSEMFNVDHIIACQVNPHVVPMLkisvts 445  Pichia guilli...
CAG87780      369 SCSLPGVFpSTLIYEKVPNTNGVQEWNnda----smKFVDGSVDNDLPITRLSEMFNADHIIACQVNPHVVPILkvsiss 444  Debaryomyces ...
Feature 1                                                                                         
Q12043        416 vggeiekeitarfrnqvtkifkFFSDETIHFLDILKELEFhpYLMTKLKHLFLQQYSGNVTILPDLsmvgQFHEVLKNPS 495  baker's yeast
NP_984327     449 vggevedefsarlkqnlstvynFVVNEVIHLLELGTELGIarNLFTKMRSVLSQQYSGDITILPDLkmllRIKELLANPT 528  Ashbya gossyp...
XP_451336     442 vggeiedefsarfkmalgkvykYMASEVIHALEIATEIGIgtNVLTKLRSVLSQQYSGDITILPEMntllRFNELLSNPT 521  Kluyveromyces...
XP_505230     452 anpdsvd--eiytlklwhnfktLVTDEVMHQLQVLYEFGIfkNLCSKMGGVLSQRYKGDITILPQVhl-sELPGILTNPT 528  Yarrowia lipo...
Q9Y827        368 nwv----------ercckkaidLAAQEVSLTFRLFAELGIfsVLFTKLQSVITQKYSGDITIIPRLny-rEVNKVIKNPT 436  fission yeast
XP_001527475  460 iggdvendlsyklkhllnnaydFVSCEAIHYLQLLTELDIqkNLSNKLISILSQNYSGDITILPDFkv-sDFAKIFENPK 538  Lodderomyces ...
XP_001382886  395 vggeienelsyrfkhllnnvydFVSCEIIHYLQILNVVDVykNLSNKLISVLSQNYSGDITILPDFki-qDFAKIFENPT 473  Pichia stipit...
XP_001645729  474 vggdiedelsakikqgltnvynYMANEAMHFLELGCEVGLakNALTKMRSVLSQQYSGDITILPDLnmlcRINKLLSNPS 553  Vanderwaltozy...
EDK38516      446 iggdydnelsykvknlcnnvynFVTSEVVHYLRVLNELNIykNLSSKLISILIQNYSGDITILPDYkv-iDFLKIFENPT 524  Pichia guilli...
CAG87780      445 iggeveneinykfknflnncydFMTSEIIHYLQILNEMDVykTLSNKLITLISQHYSGDITILPDFks-kDFFKVFKNPT 523  Debaryomyces ...
Feature 1                                                   
Q12043        496 QLFLLHqTTLGARATWPKISMIQNNCGQEFALDKAITFLKEK 537  baker's yeast
NP_984327     529 QEFLLReTVNGARATWPKIAIIKNHCGQEFELDRVINYLKGK 570  Ashbya gossypii ATCC 10895
XP_451336     522 QEFLLReATNGVRASWPKISIIKNHCGQEFELDKMINYLKGK 563  Kluyveromyces lactis NRRL Y-1140
XP_505230     529 AAYMKDtNRRGAQATYRKISLIRNHCAIELALDRAIHELKAR 570  Yarrowia lipolytica CLIB122
Q9Y827        437 PSFLLDaATRGKRGTWTKVPVTRNHCAIEILIAAAYTRLIKR 478  fission yeast
XP_001527475  539 PDFLLDfICRGAKAAWPKISIIHNHCSIEFGLDKAISILRGR 580  Lodderomyces elongisporus NRRL YB-4239
XP_001382886  474 PEFLLDfITRGARASWPKVTLIHNHCGVEFALDKAISLLRGR 515  Pichia stipitis CBS 6054
XP_001645729  554 TEFILReTTNGARATWPKISIIQNHCGQEFALERAISFLKGR 595  Vanderwaltozyma polyspora DSM 70294
EDK38516      525 TDFLLDfVIRGARSSWPKVTVIKNHCGVEFALDKAISLLRGR 566  Pichia guilliermondii ATCC 6260
CAG87780      524 PAFLLDfIIRGARASWPKVTVIYNHCGVEFALDKAISLLRGR 565  Debaryomyces hansenii

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