Conserved Protein Domain Family
Pat17_PNPLA8_PNPLA9_like4

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cd07217: Pat17_PNPLA8_PNPLA9_like4 
Patatin-like phospholipase
Patatin is a storage protein of the potato tuber that shows Phospholipase A2 activity (PLA2; EC 3.1.1.4). Patatin catalyzes the nonspecific hydrolysis of phospholipids, glycolipids, sulfolipids, and mono- and diacylglycerols, thereby showing lipid acyl hydrolase activity. The active site includes an oxyanion hole with a conserved GGxR motif; it is found in almost all the members of this family. The catalytic dyad is formed by a serine and an aspartate. Patatin belongs to the alpha-beta hydrolase family which is identified by a characteristic nucleophile elbow with a consensus sequence of Sm-X-Nu-Sm (Sm = small residue, X = any residue and Nu = nucleophile). Members of this family have been found also in vertebrates. This family includes subfamily of PNPLA8 (iPLA2-gamma) and PNPLA9 (iPLA2-beta) like phospholipases from human as well as the Pat17 isozyme from Solanum cardiophyllum.
Statistics
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PSSM-Id: 132856
View PSSM: cd07217
Aligned: 5 rows
Threshold Bit Score: 540.544
Threshold Setting Gi: 145224873
Created: 11-Feb-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
active sitenucleophile
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the crystal structure of semet patatin (1OXW)
  • Comment:catalytic dyad is formed by Ser and Asp
  • Comment:oxyanion hole is formed by two Gly and one Arg

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                ## #                                     #                              
YP_935264     15 KKILACDGGGILGLVSVEILTRLETdlrr-eldqPELVLGDWFDFTCGTSTGAIIAACIAaGMSMARIGRFYTESGGQMF 93  Azoarcus sp. BH72
ZP_01085197    7 KKILACDGGGIRGLISVEILARLEHdlqq-slgqPDLLLGDYFDFICGTSTGGIIAACLSsGMSMSQVRDFYVNNGASMF 85  Synechococcus s...
YP_001959791  13 KKILTLDGGGIRGMITIEVLGKIEQllrkqlnkgPDFVLADYFDFVAGTSTGAIIATCISlGMPVEDIKSFYTKNGRAMF 92  Chlorobium phae...
ZP_03156666   11 KRILSLDGGGIRGTLSAKILVEIEKalqt-hyqdPLYRLGDFFDLVGGTSTGSILAAGIAkGLSTTDLLSLYELDGVNIF 89  Cyanothece sp. ...
YP_001135551  16 KRILALDGGGIRGALTLGYLGRMEDilrdrhgddPEFRLCDYFDLIGGTSTGSIIATGLAlGFAVDELVDLYRSLGEKVF 95  Mycobacterium g...
Feature 1                                                                                        
YP_935264     94 DRaalfkrlrysyddepla---aklrsefdaalghqpsTAEPHATLGSPALRTLLMMVMRNhsTDSPWPVCNNPDARYnd 170 Azoarcus sp. BH72
ZP_01085197   86 ERakwymklhqnyeaeplalllqqaltcqlngpgsifnESAPPVELGDPRLRGLLMLVLRNhsTDSPWPVCNNPLAKYnq 165 Synechococcus s...
YP_001959791  93 DKafllnrlffnky-------------kheklarklrnTFGAKTTLGSDTLRTVLMMVMRNatTNSPWPISNNPFAKYne 159 Chlorobium phae...
ZP_03156666   90 QKhwlaqipglrkly----------nqydptnlekkllEVFQETTLGDSTLKCYLSITTKNatTGQTRFFDNNQESYLys 159 Cyanothece sp. ...
YP_001135551  96 DKsrlrlglfgak--------------fpkepllraldTHFGDMTLGGDELRTGLMIMTKRldTGSPWLLHNNPRGKYfd 161 Mycobacterium g...
Feature 1                                                           #                            
YP_935264    171 sg-----rrdcNLRLPLWQLVRASTAAPTFFPPEVVSFAegtpqayrfVFVDGGVTTYNNPAFLAFQMATarpyaINWPT 245 Azoarcus sp. BH72
ZP_01085197  166 ld-----rkdcNLHLPLWQLVRASTAAPTFFPPEMVSFApgtdreyqfVFVDGGITTYNNPAYLAFQMATakpyhINWKT 240 Synechococcus s...
YP_001959791 160 svrrntfphdcNLDMPLWKLVRASTAAPTYFPPEVIKVGsk-----efIFVDGGVTMYNNPAFQAFLMATvepynMNWQV 234 Chlorobium phae...
ZP_03156666  160 ----------eNSQVKLRDIVRASSAAPTFFPPHRFTIGqr-----ayEFIDGGVSLYNNPSFQLFLQAYek-dkLGWEV 223 Cyanothece sp. ...
YP_001135551 162 gdg----gsypNRDLLLRNIVRASTAAPHYFEPETLRIApgv----tgAFVDGGVSPYNNPALQMLMLATcsgygLNWRF 233 Mycobacterium g...
Feature 1                                                                                        
YP_935264    246 GTGQLLVVSVGTGTAPRlrpdlqaddlwlldhaknIPGALMNAASAGWDMACRm-----LGECRHGGLLDREIGTMvsap 320 Azoarcus sp. BH72
ZP_01085197  241 GVDQLLIVSVGTGNAPAarpdlrpeelwlldhaktIPSALMNAAIAGWDMVCRv-----MGECRFGGEVDREFGAMiqep 315 Synechococcus s...
YP_001959791 235 GENNLLVVSVGTGYCANankdlkpsemnilynassIPSALMFAASNEQDFLCRv-----FGDCKAGDLIDREVRTMmgkq 309 Chlorobium phae...
ZP_03156666  224 GADKLLLVSIGTGFAYEnipvgkaanytaldwapyLVTRLMDDANVGQNQILQlisyqpNRFARKTNKVVKEQTFPhvda 303 Cyanothece sp. ...
YP_001135551 234 GAENLMLVSAGTGHRPLrmtaaqvtdmpavvlaaqSMLSIMADANWLGQAVLQw-----LASSPTSWQIDSEIGDLrddr 308 Mycobacterium g...
Feature 1                                                                      
YP_935264    321 ge---ppnwsGPKLFSYVRYDPDV----SRAGLDALGLSdv--aeADVQRLDAIDRLPAIQR 373 Azoarcus sp. BH72
ZP_01085197  316 ltpadqsnwtGAKQFAYVRYDPLT----NAEGLIEIGLA------DVSLKEFETMSMEEKQR 367 Synechococcus sp. WH 5701
YP_001959791 310 -------gpvSPKLFTYLRYDVEL----SRQELNVLCLKdi--lpENVQKMDSVDHMEELQR 358 Chlorobium phaeobacteroides BS1
ZP_03156666  304 -------eklKDKFLTYYRFNASF----EESRLKALGLSpspqqlKALKKMDCVDQINFLQQ 354 Cyanothece sp. PCC 7822
YP_001135551 309 -------lgpGPDLITYQRYEVSLepnwLRDTLDVHIDD------EQCDALYAMDNPKNLTR 357 Mycobacterium gilvum PYR-GCK

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