Conserved Protein Domain Family
Pat17_PNPLA8_PNPLA9_like3

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cd07216: Pat17_PNPLA8_PNPLA9_like3 
Patatin-like phospholipase
Patatin is a storage protein of the potato tuber that shows Phospholipase A2 activity (PLA2; EC 3.1.1.4). Patatin catalyzes the nonspecific hydrolysis of phospholipids, glycolipids, sulfolipids, and mono- and diacylglycerols, thereby showing lipid acyl hydrolase activity. The active site includes an oxyanion hole with a conserved GGxR motif; it is found in almost all the members of this family. The catalytic dyad is formed by a serine and an aspartate. Patatin belongs to the alpha-beta hydrolase family which is identified by a characteristic nucleophile elbow with a consensus sequence of Sm-X-Nu-Sm (Sm = small residue, X = any residue and Nu = nucleophile). Members of this family have been found also in vertebrates. This family includes subfamily of PNPLA8 (iPLA2-gamma) and PNPLA9 (iPLA2-beta) like phospholipases from human as well as the Pat17 isozyme from Solanum cardiophyllum.
Statistics
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PSSM-Id: 132855
View PSSM: cd07216
Aligned: 24 rows
Threshold Bit Score: 280.728
Threshold Setting Gi: 85114361
Created: 10-Feb-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
active sitenucleophile
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the crystal structure of semet patatin (1OXW)
  • Comment:catalytic dyad is formed by Ser and Asp
  • Comment:oxyanion hole is formed by two Gly and one Arg

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                 ## #                                                      #             
CAD70812       16 LRILSLDGGGVRGYSMFLIIQELMHRTfveieg------------raprrsEIPKPCDHFDLIVGTGTGGLIAIMLGRLR 83   Neurospora cr...
XP_001931307  216 KILLSIDGDGIRGLSALLLVESLVNAIcvk-------------------vgQRLDSHQIFDLTGGSSLGGVIAIMLCRLR 276  Pyrenophora t...
XP_001799943   44 DLILTLDGGGIRGYSSLLILKQLMHEVaecerrlqrdegpvagstrqkfdeNELLPCHYFDYMYGTSTGGLISVMLARLR 123  Phaeosphaeria...
XP_391350       9 LKFLSLDGGGVRGLSSLFILKNVMERVgsk------------------mkrRDLQPYQYFDLIGGTSTGGIIALMLGRMR 70   Gibberella ze...
XP_391605      13 KRLLALDGGGVRGIMGLVILKELMHRVqkrk-----------------nlpEIPRPSDYFELAGGTSTGGIMGIMLFRLR 75   Gibberella ze...
XP_001908803    8 INLLSFDGGGVRGVTSLLIIHEIMIKIkerh-----------------glaEIPKPCDVFHMIAGTSTGGLIAIMLGRLR 70   Podospora ans...
XP_001226359   16 LRILCIDGGGIKGYTALLILRRIFRTLsadm-----------------ggnLSPRPCDIFDLIAGTSTGGIIAVMLGRLH 78   Chaetomium gl...
XP_001817389   29 LRILSLDGGGVRGLSSLCILREVMQRLsp--------------------egKPRKPCEVFDMIAGTSTGGLCAIMLGRLE 88   Aspergillus o...
XP_001875690  395 LRLLSLDGGGVRGISSLYVLKAVMNKItg---------------------dPNAKPCDYFDMIAGTSTGGLIALMLGRLR 453  Laccaria bico...
XP_001228508  107 LLKSLGNGGGIRGLSELLILNEIMSRLksrf-----------------dskEDLLPADVFHMIGGTSTGGLVALLLGRLR 169  Chaetomium gl...
Feature 1                                                                                         
CAD70812       84 lDLETCKELYVRLTRKVFEtdktiagipyrsti-----------------fkatkleeaiqdcvREHTINDREgndgavs 146  Neurospora cr...
XP_001931307  277 mQAYRAREAYKQIAKQVFCnkrdyfkyldphahsrtv----------ddtaleneiknviqqelGNPDEILLDvr----- 341  Pyrenophora t...
XP_001799943  124 mTVPQCLDIYRRVGHDLFGhrrnilplatkyhhk---------------plenavreivaefckNHEDCDGDDwhpwspe 188  Phaeosphaeria...
XP_391350      71 mSIDDCITEYQRLGSIVFGkrrhgeymfdekv-------------------lvretkavvlkylGNEDAPLLDple---- 127  Gibberella ze...
XP_391605      76 mTVDDTITEYDRIAKSIFSpkfwgydiswlpgakginnskalvqdsrfdalsmskaidevvekyGLDENDKKLkgnap-- 153  Gibberella ze...
XP_001908803   71 mSTEEALRQYDVCAENIFSskkwanitekfrs--------------------tpminiiqklvaEKDMGEMMRdpa---- 126  Podospora ans...
XP_001226359   79 mTIDECIEVYERLGKDVFGrpvggqvgrvlrgmtssp---------fydiadlqqsirsvlrarGIESDEPFIere---- 145  Chaetomium gl...
XP_001817389   89 mTVDECIEAYNQFMKKIFNvsslrkntrlvwkgcrf-----------gadnieavikelinerlGDSEAPLLNeh----- 152  Aspergillus o...
XP_001875690  454 mSIDECIEAYNTLASKIFSagllnkikdgvdtgary-----------saevleqavkdvikkysGSEDTPMRDpv----- 517  Laccaria bico...
XP_001228508  170 lTVPEAIAHYTSLAETIFTarkpfwkdgrfkasal--------------edsikrvveqtlgnaNEKMDAGNGlr----- 230  Chaetomium gl...
Feature 1                                                                                         
CAD70812      147 dtfsplsavsrssaassyprrhasnasvvsfsarspsaqsakpafssrwgnpharlyderenRTKTAVTAMYKgsprNAK 226  Neurospora cr...
XP_001931307  342 ------------------------------------------------------------pdSGDVFAITTQMdi-gTNR 360  Pyrenophora t...
XP_001799943  189 ddepvaapaktpes---------------------------slanssmwsatvtkpkkpterMCQSICLTATHsg-qIGE 240  Phaeosphaeria...
XP_391350     128 ------------------------------------------------------------ddACKTVVYTLPFknavQQS 147  Gibberella ze...
XP_391605     154 ---------------------------------------------------------lmhegGARMFCCTTAQn---RAE 173  Gibberella ze...
XP_001908803  127 -----------------------------------------------------------kpaKGKAVVCTMPRkranPKN 147  Podospora ans...
XP_001226359  146 ------------------------------------------------------------gpGCKVILCATRVe---TGK 162  Chaetomium gl...
XP_001817389  153 -------------------------------------------------------------gQCKAFVLAVRQdaanSKG 171  Aspergillus o...
XP_001875690  518 -------------------------------------------------------------dGCKVFVVACRAddlsNRI 536  Laccaria bico...
XP_001228508  231 -------------------------------------------------------------tRCKTFVCAVDTkc-vNKV 248  Chaetomium gl...
Feature 1                                                                             #           
CAD70812      227 PAVLRSYDSRkepa-----------pefDCTIWQAGRATCAIGLAFKPiqi-----------gqsIFHDDGAGVFNPSMY 284  Neurospora cr...
XP_001931307  361 AALIRSYQTRritgp---------dldqNMPIWKAMKATSAASRYVQSepgfpq-----rfviekGLVDHGTSKNNPVRD 426  Pyrenophora t...
XP_001799943  241 AYLLRSYNHTydqntfppwaltynqgadKLKIWQVTRATSAAPFYFDMlvadiknertgykyekvGFKDGGIRENNPSFA 320  Phaeosphaeria...
XP_391350     148 ATALRTYINEdkdp-----------npkKWTIWEAVRATSAATTIFEPfihgp-------pgkqiRYIDAGFGYNNPSDL 209  Gibberella ze...
XP_391605     174 SMLMRSYKDStiyskskvn-dallkhgdKLTISIAARATSAAPTFFPEvkfpe-------dkpdlVFWDGGLLNNNPIDQ 245  Gibberella ze...
XP_001908803  148 VRRIRSFSPEkdn------------wdkEVKIWEAARATTAATFYFKPqpltvstp--qggskieDYIDAAFGVNNPTNE 213  Podospora ans...
XP_001226359  163 ADVLRNYKSGhpta-----------enySCRIWEAASATAAAPMYFKSvkfa---------sggeRWCDGAIRRNNPIDE 222  Chaetomium gl...
XP_001817389  172 PVHLRSYFNTqqks-----------llpNVKAWEASRATSAAPTYFPPmevstd------kgvkhKLIDAALGANNPVGW 234  Aspergillus o...
XP_001875690  537 ATHLRTYINGnvek-----------swaDYKIWEASRATSAAPTYFPRmkl-----------gdyEYIDGGMGFNNPVLL 594  Laccaria bico...
XP_001228508  249 PTLFRSWAPKkda-------------shNCAIWQAARATTAAPPLFKQivige-------ptgtkAYVDGALGCNNPIRC 308  Chaetomium gl...
Feature 1                                                                                         
CAD70812      285 ALDEAvvne------wpgrEVGVFVSIGTGKRPKgsdmnstlwye---gfmgefadarrKLIAKIEGCEKIHEMMkkehl 355  Neurospora cr...
XP_001931307  427 ILFECrklf------ryanDMMIVVSVGTGVGINrnn-------------------eipEMANSVEDRKSEARAWsekfe 481  Pyrenophora t...
XP_001799943  321 AYSEHgsmh------gddsEPGLLLSIGTGRPNTendgfaai---------wpgplgkvKLLQKWSEKFAVFKNVlytt- 384  Phaeosphaeria...
XP_391350     210 ILQEArslwegdhylslhtDVGVFLSLGTGMGEIvrmdndtvmegl-sakirapvkaieVMKNIVTGTATIHRNVaeqf- 287  Gibberella ze...
XP_391605     246 LWYTRfelvdp---sepapSISCVISLGTGYVSPgkakpswi----------kvvgvasKVMDFATNTNAKGKDFsrhmk 312  Gibberella ze...
XP_001908803  214 LIKEAvaef------gpsrRLGCLISIGTGTKQErvisraasglrnvcdmwgsifdvgmAMKDMVTNAELVHFDLemrl- 286  Podospora ans...
XP_001226359  223 ALAELarepe-----wrnrEIGCILSLGTGLARSrsvssn-------------lasflkGALKMLTDAEDTAKVFsasal 284  Chaetomium gl...
XP_001817389  235 LWNEVlsvf------gagrPIDCILSIGTGIPKNqvfges-------------vkgaitGLGSAITNTELANILFrtlid 295  Aspergillus o...
XP_001875690  595 LMGEAhlyy------gfarPFGCLVTIGTGMAPNvslapqgvela---tnvvgaavlleGMATLTTNAEHANKLAepl-- 663  Laccaria bico...
XP_001228508  309 VISEAvtef------gvdrLVGCILSIGTGLHRAsalprpigye------rvvpvalmkVVGRLVTDGEEEAGRMeaqfa 376  Chaetomium gl...
Feature 1                                                                              
CAD70812      356 v---------------krgvnIENYYRFNVEVGvgef------------gmnEWHRLSDISTNTRRYLQ 397  Neurospora crassa
XP_001931307  482 teh-----------salmernWMKYFRFDVTGLedv-------------pleEWCHEDLIMKKTSEYLA 526  Pyrenophora tritici-repe...
XP_001799943  385 ------------------rgeHRWYKRLNVDKGlqdl------------kldNWEKGSWTNPQTGKTEV 423  Phaeosphaeria nodorum SN15
XP_391350     288 ------------------ginSVRYYRFNVDHGleav------------klfDYKKTEDMEVDTLAYLD 326  Gibberella zeae PH-1
XP_391605     313 hl------------nareehkDTKYIRFNPYLKeei-------------gldDYTRMEELKDLARKTMA 356  Gibberella zeae PH-1
XP_001908803  287 ------------------rrkPDAYFRFNVPLAadev------------slaQYGKIGKLKSMTAKYLC 325  Podospora anserina DSM 980
XP_001226359  285 g---------------rqlaqTCRYYRFNVPHGmedl------------qldEWKATERMKALTTEYLS 326  Chaetomium globosum CBS ...
XP_001817389  296 a--------------yapesrRLKYIRLNVGREiedwpe--------damekENEELAEMDSLTQIDGF 342  Aspergillus oryzae RIB40
XP_001875690  664 -------------------naVGTYYRFNVGKKiqekrwvekvepdlfskwfKGEKPEEIEHFTPENWA 713  Laccaria bicolor S238N-H82
XP_001228508  377 hsqlspafggvmwidgsstatIKQSFLQAVRHMlpei------------gknDNLEPDLAVCSVLEEIA 433  Chaetomium globosum CBS ...

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