Conserved Protein Domain Family
Pat17_PNPLA8_PNPLA9_like2

?
cd07215: Pat17_PNPLA8_PNPLA9_like2 
Patatin-like phospholipase of bacteria
Patatin is a storage protein of the potato tuber that shows Phospholipase A2 activity (PLA2; EC 3.1.1.4). Patatin catalyzes the nonspecific hydrolysis of phospholipids, glycolipids, sulfolipids, and mono- and diacylglycerols, thereby showing lipid acyl hydrolase activity. The active site includes an oxyanion hole with a conserved GGxR motif; it is found in almost all the members of this family. The catalytic dyad is formed by a serine and an aspartate. Patatin belongs to the alpha-beta hydrolase family which is identified by a characteristic nucleophile elbow with a consensus sequence of Sm-X-Nu-Sm (Sm = small residue, X = any residue and Nu = nucleophile). Members of this family have been found also in vertebrates. This family includes subfamily of PNPLA8 (iPLA2-gamma) and PNPLA9 (iPLA2-beta) like phospholipases from human as well as the Pat17 isozyme from Solanum cardiophyllum.
Statistics
?
PSSM-Id: 132854
View PSSM: cd07215
Aligned: 7 rows
Threshold Bit Score: 521.966
Threshold Setting Gi: 160878290
Created: 10-Feb-2009
Updated: 2-Oct-2020
Structure
?
Aligned Rows:
 
active sitenucleophile
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the crystal structure of semet patatin (1OXW)
  • Comment:catalytic dyad is formed by Ser and Asp
  • Comment:oxyanion hole is formed by two Gly and one Arg

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1               ## #                                    #                                
ZP_01690449    8 RVLSIDGGGIRGVLPGQIMVAIEQQLQkkTNNPEARLADYFDLMAGTSTGGILCAIYLTPdes------grpKYTAEQAV 81  Microscilla mar...
YP_678618      6 RILSIDGGGIRGIIPATVVVYMENRLQqlTKNPDVRISDYFDFVAGTSTGAILACTYLCPaaag----eavhKFTAADAL 81  Cytophaga hutch...
ZP_01118599    5 RILSIDGGGIRGILPGVVLAQIEDKLQkkTGDPEIKLADMFDFMAGTSTGGILTLAYLTPnkk------nrpKLTAQEAV 78  Polaribacter ir...
ZP_01693129    6 RILSLDGGGTRGVIPATILVYVEEQLKkkSGNPNTKLADYFDFVAGTSTGGMLACFYLAPgfsddqdktstaKFEATQAL 85  Microscilla mar...
YP_857433      4 RILSIDGGGIRGILPGQILVSLEEKLKskSHNTSARIADYFDLVAGTSTGAILSAAYVCPnee------grpKYSAKEAV 77  Aeromonas hydro...
YP_001557258   5 KVLAIDGGGMKGIVSAVLLRSLEDRLQyhSNNYKARIADYFDLIAGTSTGSILTALYLFPner------gesKFSAKEVL 78  Clostridium phy...
ZP_03275874    6 RVLSIDGGGLRGLIPAEILVSVEHKIQekTGNPQARLVEYFDLFAGTSAGGIMTCLYLSPdlqd----ptkpRCSAEEAR 81  Arthrospira max...
Feature 1                                                                                        
ZP_01690449   82 NLYLENGGDIFKkkmfsf-----ggitneKYPSAPMEEALekyLGNAKLSEMIKECLITSYDIeRSNPHFFKRHkaidnk 156 Microscilla mar...
YP_678618     82 RFYLEKGNYIFSadiwnkvt-smggflkaRYPHQPLEKVLnvaFKQSKVSELLKPCLITSYDVeRKTPVFFQSHvaqqne 160 Cytophaga hutch...
ZP_01118599   79 NIYLDRGDEIFDaniwqkik-sangvtdeKYDASELEEALqdtFGDLKLSNLLKPCIITSYDIrNGKPHFFKQHkaan-e 156 Polaribacter ir...
ZP_01693129   86 DFYVQRGNDIFNksrknnwlglrslidatKFSPIMLDNVLlevFGNTRMTELIRPCLVTTYNMkTTSPFFFNSHedtd-k 164 Microscilla mar...
YP_857433     78 NFYLEDGDEIFDvkfwrsig-tlggtsdeKYSAKELERVLmeaFGETKLSELLKPTCFVSYDVdRREPRIFKQHtaik-n 155 Aeromonas hydro...
YP_001557258  79 ESYYEYGEYIFKrqkfy-------pfwgpKYTNKYLEEMLlkyFGDATLGSLRKPCLMTSYDTtTRSAVFFNSVtgrkde 151 Clostridium phy...
ZP_03275874   82 NFFYQNSRNIFYqpcshaik-nfwgllneKYSHEKFELMMqnfFGDLKLSELLKPSLICSYEIsRRKTHFFTQHdavvsp 160 Arthrospira max...
Feature 1                                              #                                         
ZP_01690449  157 gyDFYMRDVARSTSAAPTYFEPnhatsf--aevkyALIDGGVYVNNPTLCAYAAtrkldfgedkikpTASEMMMVSIGTG 234 Microscilla mar...
YP_678618    161 alDFYVKDVCRAAASAPLYFEPsridsl--tgisyTLIDGSVYANNPTLCALTEasvlfa-ekgiplTADQFEVVSLGTG 237 Cytophaga hutch...
ZP_01118599  157 iyNFKIKDVARATSAAPTYFETariknd--igtpyPLIDGGVFVNNPALVAYSEvrtmqfenieekiSAKNMLLISIGTG 234 Polaribacter ir...
ZP_01693129  165 qrDFYVRDVARSTSAAPTYFPPaqinnl-itgeemFNIDGGLFAHDPTMMAYVEcrktkf-pqkehpTAKDMLILSLGTG 242 Microscilla mar...
YP_857433    156 qkDFLVRELLRGSTAAPTYFEAariystsplkqkfVLVDGGVVANDPALCAYSEavsm------gvsGIKDMIIVSIGTG 229 Aeromonas hydro...
YP_001557258 152 nrNYLLRDAILASTAAPTYFPPscfhak--dncynCLIDGGVFANNPTLCALIEamkl-----pgcdGIGDTICLSVGNV 224 Clostridium phy...
ZP_03275874  161 skDYYVRDVIRATSAAPTFFKVaairsl--gdemyTCVDGGVFANNPTLCAYAEarskl----pdnpTAKDMVILSLGTG 234 Arthrospira max...
Feature 1                                                                                        
ZP_01690449  235 STKYSYEYe-KAKDWGaiGWIKPLIDIMMKGVSQTVDYQLKqifd--avgkPDQYYRIEPKLvha---vsgMDNAGKENL 308 Microscilla mar...
YP_678618    238 RNQKVYTYe-QVKDWGglGWLNPLLDVMINGASDAIERELSvqfq--alqaTSQYHRVQPELlda---nqeMDDASPENI 311 Cytophaga hutch...
ZP_01118599  235 SVRKGYEYk-KAKNWGavGWIKPIIEIMMSGNSQTVHYHLKqlfgtlkeedQKDYHRLEPIInta---dteMDNASLENL 310 Polaribacter ir...
ZP_01693129  243 ASDIQLNQldKSNKWWlgKWATYLPQIVIDGAFDTITTQMRwlfdt-legdQKNYLRVDVPEenpgsfspdLADASPENI 321 Microscilla mar...
YP_857433    230 KKLKNYSYs-DVKDWGplGWAKPVIDITLEGGPQMTEYYLKqias---tvpNSKYFRIQPELyga---dpaLDNATRENL 302 Aeromonas hydro...
YP_001557258 225 KNTKSYTYq-KVRRFGllQWAVPIFDILMDASEQTVDYQLKkiyk--svnhQQYYYRMVLNTeee---ipkMDDCSKEAV 298 Clostridium phy...
ZP_03275874  235 DVKKGYPYe-QARNWGqfQWLLPLFDIIMTGVAETVDYQMRqiyd--atrsPQQYLRINTTLtdkn--mlpIDKSSDENL 309 Arthrospira max...
Feature 1                                 
ZP_01690449  309 INLRADGSESALdnedkINKIVDML 333 Microscilla marina ATCC 23134
YP_678618    312 ERLMHVAAACIEkhkdqLEVIVNRL 336 Cytophaga hutchinsonii ATCC 33406
ZP_01118599  311 QKLNEDGLAYISme--kVDAELDRI 333 Polaribacter irgensii 23-P
ZP_01693129  322 NKLQEVAQATLEhhrnsLDEFINHL 346 Microscilla marina ATCC 23134
YP_857433    303 EKLRDAGIRNSEvfdkqLDEIADLL 327 Aeromonas hydrophila subsp. hydrophila ATCC 7966
YP_001557258 299 HKLTLYGEKLANrekyrIDELAKRL 323 Clostridium phytofermentans ISDg
ZP_03275874  310 QAIRRVGQQLAEdyseeLDAIVDLL 334 Arthrospira maxima CS-328

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap