1OXW


Conserved Protein Domain Family
Pat17_isozyme_like

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cd07214: Pat17_isozyme_like 
Click on image for an interactive view with Cn3D
Patatin-like phospholipase of plants
Pat17 is an isozyme of patatin cloned from Solanum cardiophyllum. Patatin is a storage protein of the potato tuber that shows Phospholipase A2 activity (PLA2; EC 3.1.1.4). Patatin catalyzes the nonspecific hydrolysis of phospholipids, glycolipids, sulfolipids, and mono- and diacylglycerols, thereby showing lipid acyl hydrolase activity. The active site includes an oxyanion hole with a conserved GGxR motif; it is found in almost all the members of this family. The catalytic dyad is formed by a serine and an aspartate. Patatin belongs to the alpha-beta hydrolase family which is identified by a characteristic nucleophile elbow with a consensus sequence of Sm-X-Nu-Sm (Sm = small residue, X = any residue, and Nu = nucleophile). Patatin-like phospholipase are included in this group. Members of this family have also been found in vertebrates.
Statistics
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PSSM-Id: 132853
View PSSM: cd07214
Aligned: 7 rows
Threshold Bit Score: 479.625
Threshold Setting Gi: 31615943
Created: 10-Feb-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
active sitenucleophile
Conserved site includes 5 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:catalytic dyad is formed by Ser and Asp
  • Comment:oxyanion hole is formed by two Gly and one Arg

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                   ## #                                     #                           
1OXW_A        13 GEXVTVLSIDGGGIRGIIPATILEFLEGQLQEXDn-NADARLADYFDVIGGTSTGGLLTAXISTPNEN-------NRPFA 84  heartleaf night...
CAB80371      32 GQLVTILSIDGGGIRGIIPGTILAYLESQLQELD--GEEARLVDYFDVISGTSTGGLIVAMLTAQDQSgghsrnsNRPLF 109 thale cress
XP_001752066  11 GKRLTILSLDGGGVRGIIPATILEELEGYLQGLD--GSEVRIVDYFDLIAGTSTGGLITAMLAAPSRDn-----pKRPMF 83  Physcomitrella ...
ABK94005      14 QGFATILSIDGGGVRGIVPSVVLTALEAKLQKLDvdNKDARIADYFDFVAGTSTGGLMTAMLTTPNAE-------KRPTF 86  black cottonwood
ABK24853      13 GQFITVLSIDGGGVRGIIPAAILEFLEETLQKLD--GPDVSIADYFDVIAGTSTGGLVTAMLAAPNEN-------NRPLF 83  Sitka spruce
1916805       11 GKKITVLSIDGGGIRGIIPGIILASLESKLQDLD--GPDARIADYFDIIAGTSTGGLITTMLTAPNED-------KKPMY 81  Para rubber tree
AAD22149      22 GRLITVLSIDGGGIRGLIPATIITCLETKLQELD--GPDARIADYFDVIAGTSTGALLTSMLAAPDEN-------RRPLF 92  sorghum
Feature 1                                                                                        
1OXW_A        85 AAKEIVPFYFEHGPQIFNPsgq---------------------------------------------------------- 106 heartleaf night...
CAB80371     110 EAKEIVPFYLKHSPKIFPQprgifcgwg---------------------------------------------------- 137 thale cress
XP_001752066  84 DASQITQFYRENANKIFQKsrgpfgtf----------------------------------------------------- 110 Physcomitrella ...
ABK94005      87 AAKDIVQFYLDKSQLIFPQtteqyeddelfddeaainsvldearnqiqqyknemrnhiivdplisvlrfllnwgllpnfi 166 black cottonwood
ABK24853      84 AAKDITKFYLDNCPHIFPPttgflqs------------------------------------------------------ 109 Sitka spruce
1916805       82 QAKDIKDFYLENCPKIFPKesrdny------------------------------------------------------- 106 Para rubber tree
AAD22149      93 AAKDLTTFYLENGPNIFPQrkyvliklaavhhrssdvdsiva------------------------adapairvgwltpv 148 sorghum
Feature 1                                                                                        
1OXW_A       107 ------ilGPKYDGKYLXQVLqe-kLGETRVHQALTEVVISSFDIKTNKPVIFTKSNLANSpelDAKXYDISYSTAAAPT 179 heartleaf night...
CAB80371     138 etivrlvgGPKFNGKYLHDLVeg-fLGDTKLTQSLTNVVIPCFDIKKLQPVIFSSYQAVNNqamNAKLSDICISTSAAPT 216 thale cress
XP_001752066 111 rknlkslaGPKYKAEGLEDLLlqylDDDIFLSDMLTPVIIPAFDIKLQQPVFFSSSKAKTDvleNAPLLQVCRSTTAAPT 190 Physcomitrella ...
ABK94005     167 rkklrslvFPRYDGVKLHEIIne-eVGQKLLSDALTNVIIPTFDIKLFQPIIFSSLKAQRDkstDARIADVCIGTSAAPY 245 black cottonwood
ABK24853     110 --vfkylnGPKYSGDYLHKILkk-yLGDKRLHQTLTNVVITTYDIQIQQPAIFSTFTAKKDkltDAFLADVCIGSSAAPT 186 Sitka spruce
1916805      107 --dpihsiGPIYDGEYLRELCnn-lLKDLTVKDTSTDVIIPTFDIKLLLPVIFPSDDAKCNalkNARLADVCISTSAAPV 183 Para rubber tree
AAD22149     149 anligtmrGPKYDGVFLHDKIks-lTHDVRIADTVTNIVVPAFDVKYLQPIIFSTYEAKTDalkNAHLSDICISTSAAPT 227 sorghum
Feature 1                                  #                                                     
1OXW_A       180 YFPPHYFVTNTsng----deyEFNLVDGAVATvADPALLSISVATRLaqkdpaf---asirslNYKKXLLLSLGTGTTSE 252 heartleaf night...
CAB80371     217 FFPAHRFTNEDseg----ikhEFNLIDGGIAA-NNPTLCAIAEVTKQiikknpv--mgdisplDFTRFLVISIGTGSIRN 289 thale cress
XP_001752066 191 YFPPARFTLIDktqep-nrirEFNMIDGGIAV-NNPTYVAITQAIKElrsgglc--sdrvdyyGYDDLLILSLGTGSQVH 266 Physcomitrella ...
ABK94005     246 YFPPYYFKTKV----------DFNLADGGLAA-NNPSLLAVCEVMKEqk-------------mDGRKLLILSLGTGAADQ 301 black cottonwood
ABK24853     187 YLPAYYFQTKDssg----ntrDFNLVDGGIAA-NNPTLLAINQVTKQtlvdcpgfavptskasDTTKFLVLSLGTGQKME 261 Sitka spruce
1916805      184 LLPAHSFTTEDdkn-----ihTFELIDGGVAA-TNPTLLALTHIRNEiirqnpr--figanltESKSRLVLSLGTGKSEY 255 Para rubber tree
AAD22149     228 YFPAHYFTTEPagpgdarpprEYHLVDGGVAA-NNPTMIAMSMLTKEvlrgnpd--fnpgkptEYRNYLVISGPPSLAEK 304 sorghum
Feature 1                                                                                        
1OXW_A       253 fdktyt------akeaatWTAVHWXLVIQKXTDAASSYXTDYYLSTAFQALdSKNNYLRVQENALTGttTEXDDASEANX 326 heartleaf night...
CAB80371     290 qekynakm--askwglmcWVFESGSTPILDCYSEAIHDMVDYQSSVVFQALrSEKNYLRIDDDSLKGdlGSVDISTEKNM 367 thale cress
XP_001752066 267 sydaeev----akwgvvdWMVHDGETPLVDMVFNASSDMVDYNLSIIFESQdSSKNYLRITTDSLERsaVKLDDASKSNM 342 Physcomitrella ...
ABK94005     302 sgryvvgdpskwgllrwlWYSENNGSPLIDILTTAPDEMISTYISTIFKYCgWEDNYYRLQAKMELTg-ARMDDASQENL 380 black cottonwood
ABK24853     262 tynatda----akwgligWLSNGGKTPIIDMFSQSSADMVDIHASVVFQAFkSEKNYLRIQVELSGDt-SSTDLSTKKNL 336 Sitka spruce
1916805      256 kekynadm--tskwrlynWALYNGNSPAVDIFSNASSDMVDSHLSALFKSLdCEDYYLRIQDDTLTGeeSSGHIATEENL 333 Para rubber tree
AAD22149     305 ytapqca-----kwgliqWLYEGGFTPIIDIFSHASADMVDIHAAVLFEALhCEKNYLRIQDDSLTGhaSSVDIATKENM 379 sorghum
Feature 1                               
1OXW_A       327 ELLVQVGENLLKKPVSEDNPETY 349 heartleaf nightshade
CAB80371     368 EGLVEVGEALLKKRVSRVNLESG 390 thale cress
XP_001752066 343 DKLVKTAQKLLENPVAQRDFNTG 365 Physcomitrella patens subsp. patens
ABK94005     381 RKLEKIGKDLAAKHDAELEALAQ 403 black cottonwood
ABK24853     337 EDLVELGRKLLDDSVSRVNIETG 359 Sitka spruce
1916805      334 QRLVEIGTELLEKQESRINLDTG 356 Para rubber tree
AAD22149     380 EALIGIGKKLLKKPVARVNIDTG 402 sorghum

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