Conserved Protein Domain Family
Pat17_PNPLA8_PNPLA9_like1

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cd07213: Pat17_PNPLA8_PNPLA9_like1 
Patatin-like phospholipase
Patatin is a storage protein of the potato tuber that shows Phospholipase A2 activity (PLA2; EC 3.1.1.4). Patatin catalyzes the nonspecific hydrolysis of phospholipids, glycolipids, sulfolipids, and mono- and diacylglycerols, thereby showing lipid acyl hydrolase activity. The active site includes an oxyanion hole with a conserved GGxR motif; it is found in almost all the members of this family. The catalytic dyad is formed by a serine and an aspartate. Patatin belongs to the alpha-beta hydrolase family which is identified by a characteristic nucleophile elbow with a consensus sequence of Sm-X-Nu-Sm (Sm = small residue, X = any residue and Nu = nucleophile). Members of this family have been found also in vertebrates. This family includes subfamily of PNPLA8 (iPLA2-gamma) and PNPLA9 (iPLA2-beta) like phospholipases from human as well as the Pat17 isozyme from Solanum cardiophyllum.
Statistics
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PSSM-Id: 132852
View PSSM: cd07213
Aligned: 16 rows
Threshold Bit Score: 319.233
Threshold Setting Gi: 154244962
Created: 10-Feb-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
active sitenucleophile
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the crystal structure of semet patatin (1OXW)
  • Comment:catalytic dyad is formed by Ser and Asp
  • Comment:oxyanion hole is formed by two Gly and one Arg

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                 ## #                                     #                             
NP_900111      6 PYRILSLDGGGLRG---IIALVILDRLDRa------aPGWRDGIHMHAGTSTGALIALGLARGm----TPRQILDQYLER 72  Chromobacterium...
CAF05653      18 KYRILSLDGGGLRT---LLSIGLLKRIDQl------rPGFLDQVDLVAGTSAGAISALIIAAArepavGLEQARQIWFTP 88  Angiococcus dis...
YP_001415920   2 TYRILSLDGGNGGKa--GVFLSTLERRAEvl-----gFSVLRQIDLFAGTSNGAVNALLLAWFddpvlAFEAVLRFDDEV 74  Xanthobacter au...
XP_653645    320 KFRILSLDGCGVRT---LVQLIILKRIVEn------fPHFLDNVDMICSVGASTLPILMTLVGy----DIDTVIKIYEKT 386 Entamoeba histo...
NP_391333      3 KYRIMTFDGGGTLG---ALSLQLLNRLARq------nPKLISRTHVFSGNSIGSFTALALASGr----SPRETLQYFEDE 69  Bacillus subtil...
XP_001749155  81 GCRILALDGLGANV---IFHISLLCRLLAq------ePEFDEQPDVYVGSSLGAVTALMLAYGy----SSRQVAQLYEHE 147 Monosiga brevic...
XP_656377    383 KFRVLSLDGGGVKC---IYQCVLLKRIIKe------iPDFFDKIDLITGLSASSLVSVSILLGl----DMEFVIKVMEAV 449 Entamoeba histo...
XP_652675    331 KYRIISLDGGGIKV---LMEVILLQRLSAe------fPDMFVNCNLFCGCSASSVVAVTLSMGy----NLDGLMKLIEHV 397 Entamoeba histo...
XP_650745    356 KFRILSLDGGGIKC---IYQCAILQRILDk------fPDFFEKIDVITGVSASSVPCVAIALGy----DLLSVEKMMEDM 422 Entamoeba histo...
YP_632470      2 PHRVLAMDGTSISGgegYVTAGLLKSLKEtltsrgggQSLLNDVELFSGTSAGSFNAAFFATYenpddAFPKILDFWGEV 81  Myxococcus xant...
Feature 1                                                                                        
NP_900111     73 GPKLFErgaarrlktlngligarydgaerericrdvlggadtlasllrdgGSRGHVLVPAFNLDGDprlp-----qgrRR 147 Chromobacterium...
CAF05653      89 GLFDSPlsnqlgalvgqsalmps----------enmaralthilgdktlrDLKRKVVIPAFQLDDGdpd------edrRG 152 Angiococcus dis...
YP_001415920  75 RESQTPwsiagtvlgnnaamttg----------riktfcdevfgagailaDVKRPVLVNTVQLDNAlsd-------gqRS 137 Xanthobacter au...
XP_653645    387 MVKVFSstshkggvlsykyssk------------fikilatkifqdkklcDLKREVVVPVIKIDSGind-------pnRR 447 Entamoeba histo...
NP_391333     70 ILPAFSisrpggpvfnqqlpysg-----------fikavrnffpadlqliDLRKRIVVPSFKLYSQk----------lDR 128 Bacillus subtil...
XP_001749155 148 MEQHLSwlptgfnwrivapfwnshs-------dhakeaflrrcfgqatlrDCQHWVAVAAFDLAGSpdeqelpspqptRH 220 Monosiga brevic...
XP_656377    450 SLETFKneshlagiaghqftnk------------flkvmgerllgnltinDLPRNCCIQSFLIDTGkdd-------tkRT 510 Entamoeba histo...
XP_652675    398 IRYSFKkdsiqsvtnskyindy-------------lrafgevafgnlklhDLPRHVLIPSFLIDSGids-------etRH 457 Entamoeba histo...
XP_650745    423 LCETFSriinkggivghqysnk------------flfvmgdkvfgdltmdKLTRKVCIPSYLTDTGkdd-------pnRT 483 Entamoeba histo...
YP_632470     82 VQMNRKavslgrtamaltgnaalld------ssymrdffcsyfgatlrlgDLKRKVALPSFQLDGHrk--------slRT 147 Myxococcus xant...
Feature 1                                                                 #                      
NP_900111    148 WKPKVYHNLptrdg-------sddgAEQAWRVAMRSSAAPTYFPSF-------DGFADGGVFANNPAMCALAqtrda--- 210 Chromobacterium...
CAF05653     153 WRPRIFHNFpgdt--------fvnlDDYLVDLALRSSSLPIVSPVH-------QGYVDGGLFANNPTMSAVAqaiy---- 213 Angiococcus dis...
YP_001415920 138 WLPRTFSNFpgd----------lgrVELIADVVLRTMAMPLQYPIYqsgaqtgPAYVDGAVASNNPALTAITsal----- 202 Xanthobacter au...
XP_653645    448 MIPVLLNNIvlkktntdlddnminnEDLLSDVVLRSFATPGLFTCH-------QSFVDGSFLINQPFAVAKAf------- 513 Entamoeba histo...
NP_391333    129 WTPVLFHNFpgs----------pylNEKVSDVILRSSGAPATQRAY-------QNYVDGYVVATNPSTASIAfavg---- 187 Bacillus subtil...
XP_001749155 221 WQPTILTNLprgrga-----hepdlDLPLVEVAMRATATPTYYAIH-------NNHIDGTVFAANPSLSGMArvla---- 284 Monosiga brevic...
XP_656377    511 SKAMLYNNFmke-----------tyDKDLKNICLQSASAPGYFDPL-------NNHVDGSLLVNNPFYLTYPliige--- 569 Entamoeba histo...
XP_652675    458 CKSEAFNNIipg-----------ndTEKVADVCLRSAAAPSYYKPY-------QNYVDGGIVDNVPCGVAWPyliga--- 516 Entamoeba histo...
XP_650745    484 SLAKFYNNFlge-----------evPYSLTEVCIQSAAAPGYFGSV-------NAHLDGGVIINEPCGGVLSyvige--- 542 Entamoeba histo...
YP_632470    148 WKAKIFHNTgpdn--------dsdhNELVVDVLMRSGSPPLSYPVYqgireqgSGYVDGGIFANNPSLVALAqiinnitr 219 Myxococcus xant...
Feature 1                                                                                        
NP_900111    211 ---------rlaraIPPESVSMLSLGTGFNASHLdg---------------------dnDWGALQWGRnl------tgLL 254 Chromobacterium...
CAF05653     214 -----------akaADVRDILVFSLGTGDSVDYLdgy--------------------neNWGWRKWLLdpkqpmafvaAT 262 Angiococcus dis...
YP_001415920 203 -----------aqqKALEDIALLSIGTPRNLAGQsrylspg------------lsegsaDWGYRQWLLdpfnplvlvdLF 259 Xanthobacter au...
XP_653645    514 --------------FDPKESVVLSLSGGTYKSYFdae-------------------kytGAGYVQWATcv------tdLF 554 Entamoeba histo...
NP_391333    188 -----------kanVPLDQIAVLSIGTGEAPTRLrrdtrgwgmvsadnirpenlknlppNWGVLLDRSpnep--llpfLQ 254 Bacillus subtil...
XP_001749155 285 ----------afpaLSVRDVSVLSLGGAVSRQYAfsa--------------------nlDWGVWQWLPwi------lkLL 328 Monosiga brevic...
XP_656377    570 ----------nglnIAKENIVALSIGSGYPTVPYydie------------------qmgNAGLIQWLIkl------sdFN 615 Entamoeba histo...
XP_652675    517 ----------kgigIDPKDIVCLSLSAGRPTPAHldaq------------------kigNGGLVQWAPvl------adLF 562 Entamoeba histo...
XP_650745    543 ----------yglnVDIKDISVLSIGAGYPAIPYipee------------------elrEAGPVKWTLhm------pdLQ 588 Entamoeba histo...
YP_632470    220 ksrtekvetletepPDLTNILMMSVGNGCMSSYLtphfc----------------kgvaNWGFSKWLLdfhdpmvlvkMM 283 Myxococcus xant...
Feature 1                                                                          
NP_900111    255 MDGVNDVADFQVRq-mLGEGRYLRVSALL-----------AEPIALDDAGRMAQMATIGGQVDLDE 308 Chromobacterium violaceum ATC...
CAF05653     263 IEAGVEAIDFQAKm-lLPRGNYWREDPVV-----------PFNLGNSVEAQIATLDRVVKRHDLSK 316 Angiococcus disciformis
YP_001415920 260 LQAGAAATSAQCAq--LLGSRFRRVDPPVft--------gPAWDAPETNAVLDTTVDWLNAHGWND 315 Xanthobacter autotrophicus Py2
XP_653645    555 KTAGIALADEFAKv--LFGPRAHRLDPKL-----------PCQIDDDSFKDIELLKTIAAKVDLSA 607 Entamoeba histolytica HM-1:IMSS
NP_391333    255 MIAGGNGYYESMVsanLLGDRFFRLDPRIpnfsktdpavvPAVIEIANKTNLQPANQFIEKNWGSK 320 Bacillus subtilis subsp. subt...
XP_001749155 329 NNATADACNNNMQq--VLQDRYLRLQPPAi---------pNLRYDHADRYVCPSFVEKQQTRPLHP 383 Monosiga brevicollis MX1
XP_656377    616 IFARKEMGVMESRm--IFKNNYFRLDPHL-----------PIEVDIADAKNIEAIKIMGKEQNLDK 668 Entamoeba histolytica HM-1:IMSS
XP_652675    563 MLARRDETVKEGKl--LFGERFLRVDPIL-----------PGQILLDNVEQIEEVKRIAQSYDLEE 615 Entamoeba histolytica HM-1:IMSS
XP_650745    589 IESRKSFIEFEGKy--LLGDRFFRLNPKL-----------PYQIDLDYCKDVELIKSMGDNLDLTE 641 Entamoeba histolytica HM-1:IMSS
YP_632470    284 LEAGSDAADYQCRm--ILRKKYFRLNPVV-----------DQTLSAANLKQVESTVQSLLSTQSTV 336 Myxococcus xanthus DK 1622

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